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Citations for New England Biolabs :
301 - 350 of 609 citations for Recombinant Human CEACAM1 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The domain deletion mutants of pCS2+ FLAG-tagged SRSF1 construct were generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The primers sequences are given in Supplementary Table S1.
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bioRxiv - Genomics 2021Quote: ... and introduced between XhoI and BamHI sites in c-Flag pcDNA3 (addgene #20011) to generate Flag-tagged hPEX5 using NEBuilder® HiFi DNA assembly Master mix (New England Biolabs). Site-directed mutagenesis for amino acid substitution was performed using the Q5® Site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: All purified SNAP-tagged proteins were labeled with 10-fold molar excess of SNAP-Surface dyes (New England Biolabs, Ipswich, MA) in labeling buffer (1× PBS ...
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bioRxiv - Microbiology 2023Quote: ... 1990) tagged with Illumina adapters in PCR reactions using a Q5® Hot Start High-Fidelity DNA Polymerase (New England Biolabs). The 25 amplification cycles were as follows ...
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bioRxiv - Immunology 2022Quote: ... Short hairpin resistant chimeric mutants of Flag-tagged GLUT3 alleles were generated using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: Plasmids (pBluescript KS+, pBS hereon) for HiBit-tagged POI mRNA were generated by Gibson Assembly (Gibson Assembly cloning kit, NEB, E5510S) or restriction digest cloning ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The biotinylated and 6xHis-tagged screens were immunoprecipitated overnight with streptavidin or Ni-NTA magnetic beads (New England Biolabs, Ipswich, MA), respectively ...
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bioRxiv - Biophysics 2024Quote: ... An in-house vector with H1.8 tagged with N-terminal eGFP tag and 6x Histidine-tag was transformed in T7 Express cells (enhanced BL21 derivative, NEB C2566I) and grown to an OD of ≈ 0.7 at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: Toxins were expressed with (His)6-tags at their C-termini in competent C43 (DE3) Escherichia coli cells (NEB) for HlgA and in BL21 (DE3 ...
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bioRxiv - Biochemistry 2021Quote: LCB1 was synthesized and cloned into E.coli using a pET expression vector via Hi-Fi DNA assembly (NEB; E2621S) with a gBlock (ITD ...
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bioRxiv - Immunology 2021Quote: ... Restriction digest of EtMIC3 PCR product was performed using Bam HI and Eco RI restriction enzymes (New England Biolabs) to extract antigen coding sequence for cloning into pYD1 plasmid vector ...
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bioRxiv - Biophysics 2020Quote: ... The point mutants were subsequently cloned in the pNZopuAHis plasmid (C-terminal 6-HIS-tag) using EcoRV-AlnwnI (NEB) for the substrate-binding domain mutants and βamHI-AlwnI (NEB ...
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bioRxiv - Biophysics 2020Quote: ... with TEV-cleavable 6x-His tag at N-terminal of the gene cloned between NheI (New England Biolabs Inc.) and HindIII (New England Biolabs Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... Par-3 PDZ1-APM and PDZ1-APMΔPDZ2 were first his-purified (described above) prior to incubation with amylose resin (NEB). Amylose-bound Par-3 was then washed and resuspended in binding buffer as described for GST proteins.
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bioRxiv - Genomics 2021Quote: ... the Hi-C library was amplified for about 10 cycles of PCR with the Q5 master mix (NEB, M0492L), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Near full-length 16S rRNA genes were amplified by PCR using Phusion Hi-Fidelity DNA Polymerase (New England BioLabs) and a bacteria-specific primer set ...
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bioRxiv - Genomics 2023Quote: Hi-C single-index library preparation of MCF7 cells was performed as previously described using MboI (New England Biolabs) restriction enzyme (30).
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bioRxiv - Neuroscience 2023Quote: ... These two amplicons were incubated with SfoI digested pET23a-His-TEV plasmid in a HiFi Assembly reaction (NEB, E2621S) to generate a plasmid where HaloTag and SpyCatcher3 is linked with a GSGESGSG linker sequence.
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bioRxiv - Molecular Biology 2022Quote: ... 404–561 with a C terminal His tag added was cloned into the pMAL-c5x vector (New England Biolabs), and were transformed in E ...
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bioRxiv - Molecular Biology 2023Quote: The following M35 derivative constructs were derived from pcDNA3.1 TOPO M35-V5/His using the Q5 site-directed mutagenesis kit (#E0554, New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... the plasmid digestion was supplemented with 1.5 units of recombinant shrimp alkaline phosphatase (rSAP; NEB #M0371S). Digested insert and vector were purified and ligated together at room temperature for 30 min using T4 DNA ligase (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... after which 2 µL of Recombinant PNGaseF (Glycerol-free) (New England Biolabs, Ipswich, MA; catalog # P0709L) was added to each sample ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant SYNJ2BP (0.5 µg) was dephosphorylated using 1 µl of calf intestinal alkaline phosphatase (CIP) (NEB) in 1x CutSmart Buffer in a total volume of 10 µl ...
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bioRxiv - Molecular Biology 2023Quote: ... the enzymatic activity of the commerically availablale recombinant vaccinia virus methyltransferase mRNA 2’O-methyltransferase (NEB), abbreviated hereagter as VMTR1 ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed in Escherichia coli T7 Express lysY/Iq cells (New England Biolabs). Except for GST-PP1γ ...
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bioRxiv - Bioengineering 2024Quote: Isothermal Calorimetry: Recombinant hSTING was synthesized in Escherichia coli (New England Biolabs, T7 SHuffle Express line) and purified by affinity chromatography ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant protein of AP2 domains fused with MBP (MBP::PbSIP2) was purified using amylose resin (NEB) and recovered in 10 mM maltose solution.
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bioRxiv - Neuroscience 2024Quote: ... and used to form ribonucleoprotein (RNP) complexes when combined with recombinant Cas9 protein (New England Biolabs). Ribonucleoprotein complexes were formed using the method of Kouranova49 ...
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bioRxiv - Microbiology 2023Quote: Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... MARCH2 chimeras containing the TM domains from either human MARCH4 or human transferrin receptor (TR) were generated using the NEBuilder HiFi DNA assembly kit (New England Biolabs) and the primers listed in Supplemental Table S2 ...
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bioRxiv - Biochemistry 2023Quote: Human HA-DHFR was subcloned from human cDNA to pcDNA4/TO by PCR using Q5 High Fidelity 2X mastermix (NEB). Human FPGS-FLAG and GGH-FLAG were subcloned from cDNA clones MHS6278-202755815 (Horizon Discovery ...
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bioRxiv - Biophysics 2020Quote: ... from human origin were all purchased from NEB. The H2A/H2B and H3.1/H4 were mixed in 2(H2A/H2B)2:1(H3/H4)4 molar ratio to form octamers.
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bioRxiv - Cancer Biology 2024Quote: ... or rabbit anti human HMGA2 (New England Biolabs), ...
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bioRxiv - Molecular Biology 2022Quote: ... Flag/V5-tagged TCF7L2 and Flag-tagged FOXQ1 truncation constructs were generated by restriction cloning using the high fidelity Q5 polymerase (New England Biolabs, Ipswich, US). For cloning of FOXQ1 truncation constructs ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins (except from cells expressing eGFP and cytoplasmically tagged EGFR) were further treated with 250 U of PNGase F (NEB, Cat# P0704) for 1 hour at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and the impurities that were tagged by Chitin Binding Domain were extracted on a gravity-flow column using 5 mL Chitin resin (New England BioLabs, Evry, France). The target proteins were purified with HisTrap HP columns (GE Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: Amplicons of tagged-Cdep were isolated by gel extraction after PCR amplification using Phusion high fidelity polymerase (New England Biolabs, catalog #E0553L). The amplicons were then cloned into the pJFRC7 5x UAS vector (ref ...
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bioRxiv - Cell Biology 2024Quote: ... SNAP tagged SNX9 or dynamin 2 were chemically labelled with SNAP-Surface Alexa Fluor 488 or 647 (New England Biolabs S9129 / S9136) and included in the FLS mix at 30 nM or 25 nM final concentration respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were amplified for 12 PCR cycles with unique dual index primers using the NEBNext Hi-Fi 2X PCR Master Mix (New England Biolabs). Amplified libraries were purified using a 1.2X ratio of Axygen magnetic beads (Corning Inc ...
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bioRxiv - Genetics 2021Quote: ... FAN1 variants were introduced in the pFASTBAC1-GST-FAN1-FLAG-His plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Confirmed by DNA sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... Vectors containing cohesin trimers were generated by combining pACEbac1 SMC1-His SMC3 with pIDC SCC1-2xStrepII by a Cre recombinase reaction (New England Biolabs). The vector for the Scc2/4 expression was also created by combining the pACEbac1 SCC2-2xStrepII with pIDC His-SCC4 using Cre recombinase.
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bioRxiv - Immunology 2021Quote: ... Restriction digest of pMK-RQ plasmid DNA containing EtIMP1 was performed using Bam HI and Not I restriction enzymes (New England Biolabs). Restriction digest of pYD1-EtAMA1Cit plasmid was performed with the same restriction enzymes to remove the EtAMA1 insert but retain the citrine tag ...
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bioRxiv - Microbiology 2021Quote: ... which were incubated with 1 μg/ml full-length S protein with His tag that had been pretreated with or without FXa (P8010L, NEB) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Tagmented DNA was amplified with 12 cycles of PCR using the NEBNext Hi-Fi 2X PCR Master Mix (NEB M0541) and unique dual index primers ...
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bioRxiv - Cell Biology 2021Quote: ... coding sequence was amplified from Arabidopsis cDNA and assembled into a pGreen0179-35S-spFLA11-His-YFP vector using NEBuilder HiFi DNA Assembly kit (NEW ENGLAND Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... open reading frames were cloned into a linearized pET28b vector with BamH1 and Xho1 in frame with a N- terminal 6x-His tag using Gibson HiFi assembly mix (NEB), 10 μL total reaction volume with 2 μL of linearized vector ...
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bioRxiv - Biochemistry 2022Quote: ... Constructs HsKHC-MDC and HsKHC-MDCL2-CaKip3 contained a cleavable C-terminal TEV-SNAP-6X-His tag (SNAP-tag from NEB). DARPin-D2 was ordered as a codon-optimized gene product from IDT and cloned into pET16b using Gibson Assembly ...
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bioRxiv - Cell Biology 2022Quote: ... The pDyn1 plasmid [the pACEBac1 expression vector containing insect cell codon-optimized dynein heavy chain (DYNC1H1) fused to an amino-terminal His-ZZ-TEV tag and a SNAPf tag (New England Biolabs) on the carboxy-terminus] and the pDyn2 plasmid (the pIDC expression vector with codon optimized DYNC1I2 ...
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bioRxiv - Biophysics 2022Quote: ... was further amplified by PCR with an overlapping sequence with pGEX-6P and cloned immediately after the PreScission site of the pGEX-6P vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Biophysics 2022Quote: ... with the (G4S)4-GGS linker was amplified by PCR by adding a linker sequence and inserted into the pGEX-6P vector by Hi-Fi DNA assembly (NEB).