Labshake search
Citations for New England Biolabs :
301 - 350 of 2232 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... Purified plasmids were digested stepwise with SacI (NEB, R3156S) to prevent exoIII digestion on the arrest peptide side and then with AvrII (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... The plasmids constructs were linearized with PsiI-v2 (NEB), transfected into the TgTBC9AID parasites along with a universal pU6 tagging to the UPRT coding region ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were linearized with KpnI-HF (New England Biolabs). Agarose gels were used to purify linear fragments ...
-
bioRxiv - Developmental Biology 2023Quote: Plasmids were constructed by using gibson assembly (NEB, E2621) to transfer lck-eGFP (80 ...
-
bioRxiv - Genomics 2023Quote: ... All plasmids were linearized by BsaAI (New England Biolabs) and co-injected into the MAC of vegetative P ...
-
bioRxiv - Genomics 2023Quote: All plasmids used were cloned using Gibson assembly (NEB). The all-in-one HER2 CAR constructs used for in vivo tumor control studies were cloned by digesting an empty lentiviral vector for constitutive gene expression (Addgene 79121 ...
-
bioRxiv - Microbiology 2023Quote: ... plasmid DNA was incubated with XhoI restriction endonuclease (NEB) at 37 °C or Bt.NstNBI nicking endonuclease (NEB ...
-
bioRxiv - Cell Biology 2022Quote: ... plasmid clones were digested by EcoRI-HF (NEB #R3101S) to confirm the insertion ...
-
bioRxiv - Biochemistry 2023Quote: ... The parental template plasmid was digested with DpnI (NEB) and the PCR amplified products were purified using the DNA Clean and Concentrate kit (Zymo) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was linearized with NotI-HF (R3189, NEB) and subjected to IVT reactions using 50 ng/μl T7 RNA polymerase HC (EP0113 ...
-
bioRxiv - Genomics 2023Quote: ... the plasmids were digested with BamHI-HF (NEB, R3136L) and EcoRV-HF (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... plasmid pMQ361 was digested with BsrGI and BamHI (NEB) to obtain the tdtomato fragment ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were transformed into NEB 5-alpha F’Iq (NEB). Standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
-
bioRxiv - Synthetic Biology 2024Quote: gRNA plasmids were generated by T4 PNK phosphorylating (NEB) and annealing two oligos ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Enzymes used for plasmid construction were purchased from NEB. Primers were obtained from IDT and Eurofins genomics ...
-
bioRxiv - Genetics 2024Quote: ... Plasmid was then digested with EcoRI and EcoRV (NEB) overnight to generate linear chromatin templates (i.e. ...
-
bioRxiv - Cancer Biology 2024Quote: ... Briefly the plasmid was digested with Esp3I (NEB#R0734) at 37°c overnight ...
-
bioRxiv - Genetics 2023Quote: ... Plasmids were double digested with HindIII-HF (NEB, #R3104S) and KpnI-HF (NEB ...
-
bioRxiv - Biochemistry 2024Quote: ... The plasmids were transformed into either BL21(DE3) (NEB) or OrigamiB(DE3 ...
-
bioRxiv - Microbiology 2022Quote: ... Clean RNA was first rRNA depleted using the NEBNext rRNA depletion kit (Human/Mouse/Rat) (NEB) before being prepared for sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) according to the manufacturer’s instructions with 6 µl total RNA used as input per sample ...
-
bioRxiv - Neuroscience 2020Quote: ... Mutations were generated in the rat P2X2 receptor using the Q5 Site-Directed Mutagenesis Kit (NEB). Each mutation was verified by DNA sequencing and subcloned in pWPT-EF1α-P2X2-GCaMP6s-IRES-DsRed2 or pWPT-EF1α-P2X2-GCaMP6s-P2A-mScarlet lentiviral vectors.
-
bioRxiv - Microbiology 2022Quote: ... sequences of RcACE2 or hACE2 were PCR-amplified from pCAGGS-RcACE2- or pCAGGS-hACE2-expressing plasmids (10) and cloned into EcoRI- and XhoI-digested pCAGGS-blast plasmids using NEBuilder (New England Biolabs, Ipswich, MA, USA). Vero/TMPRSS2 cells were transfected with pCAGGS-blast-RcACE2 or pCAGGS-blast-hACE2 plasmids using the PEI MAX transfection reagent (Polysciences ...
-
bioRxiv - Genomics 2023Quote: ... and single colonies were picked 1 day later to grow up and extract plasmid DNA using a Monarch Plasmid Miniprep Kit (New England Biolabs, Cat. No. T1010L). Extracted plasmid DNA was sequence confirmed via long-read Nanopore sequencing (Primordium Labs ...
-
bioRxiv - Biophysics 2021Quote: A TetR-mCherry-antiGFPnanobody plasmid was constructed based on a pIRES-CMV-LS-dCas9-mCherry-antiGFPnanobody plasmid (Dahan lab) and a TetR-GFP plasmid (Dahan lab) through amplifications by Q5® High-Fidelity DNA polymerase (NEB, Cat. No M0492S) (primers ...
-
RTEL-1 and DNA polymerase theta promote subtelomeric DNA synthesis and telomere fusion in C. elegansbioRxiv - Genetics 2022Quote: ... and pELS53 [Peft-3::Cas9 + rtel-1 sgRNA] plasmids were generated for this study by modifying the pDD162 plasmid (Dickinson, 2013) using Q5 Site Directed Mutagenesis Kit (NEB, Cat# E0554S, Ipswich, MA).
-
bioRxiv - Zoology 2023Quote: ... The recombinant clones containing these constructs were then grown overnight in LB liquid media containing 25 μg/ml of Zeocin™ antibiotic and plasmid DNA isolation was isolated using the Monarch® Plasmid Miniprep Kit (New England Biolabs, Whitby, ON). Base-pair accuracy and orientation of the receptor constructs were confirmed by Sanger sequencing (Center for Applied ...
-
bioRxiv - Molecular Biology 2022Quote: ... We then carried out rRNA depletion with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat NEB # E6310S) using the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
-
bioRxiv - Synthetic Biology 2021Quote: ... All plasmid-template PCR products were treated with DpnI (NEB) following standard protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Plasmid pIW-IRES was digested with BamHI-HF (NEB, R3136S) and ApaI (NEB ...
-
bioRxiv - Plant Biology 2022Quote: ... the plasmids were digested with restriction endonuclease PI-PspI (NEB), and the fused EGFP expression cassettes from the digested plasmids were integrated into the pRCS2-ocs-nptII vector for transformation (Chung et al ...
-
bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was purified with mini-prep kits from NEB. Screening PCRs were performed using DreamTaq polymerase (Thermo Fisher) ...
-
bioRxiv - Genomics 2020Quote: Each plasmid miniprep sample was amplified by Phusion (NEB M0530L) to generate libraries of the mutagenized HSF1 region flanked by cluster generating Illumina adaptors ...
-
bioRxiv - Molecular Biology 2021Quote: ... The P16 plasmid was amplified using Phusion polymerase (NEB M0530S) for the backbone (forward primer ...
-
bioRxiv - Immunology 2019Quote: ... pSV40-CLuc control plasmid was obtained from NEB (Ipswich, MA). These constructs were co-transfected to 293 cells by FugeneHD (Promega) ...
-
bioRxiv - Genomics 2019Quote: ... The plasmid was linearized using EcoRV (New England Biolabs, R3195) and purified from agarose gel (Zymo Research ...
-
bioRxiv - Genomics 2020Quote: ... the plasmid was treated with calf intestinal alkaline phosphatase (NEB) for 30 min at 37 °C and gel purified with a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel) ...
-
bioRxiv - Immunology 2020Quote: All new plasmids were assembled using NEBuilder kit (NEB # E5520S) following the manufacturer’s recommendations unless otherwise noted ...
-
bioRxiv - Genomics 2021Quote: ... The sequenced plasmid was transformed into C3013 competent cells (NEB) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... then isolated using a Monarch® Plasmid Miniprep Kit (NEB). The isolated products were then sequenced to ensure proper addition of the CAD gene sequence to the MigR1 backbone.
-
bioRxiv - Bioengineering 2021Quote: ... Digested plasmid backbones were treated with rSAP (New England Biolabs) for the final 5 minutes of the digestion ...
-
bioRxiv - Biochemistry 2020Quote: ... Sequence-verified plasmids were transformed into BL21 (DE3) cells (NEB). Starter cultures (5 mL ...
-
bioRxiv - Neuroscience 2021Quote: ... 5µg of lentiviral CRISPR plasmid was digested with BsmBI (NEB) for 90 min at 37°C ...
-
bioRxiv - Biochemistry 2021Quote: ... pRZ-R3C plasmid was treated with EcoRI-HF (NEB, USA). RNA was expressed using T7 RNA polymerase (homemade ...
-
bioRxiv - Biochemistry 2020Quote: ... the original plasmid was digested with DpnI (New England Biolabs) for 2 hours at 37 °C ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Background plasmid was digested using Dpn1 (New England Biolabs, R0176L), and the remaining PCR product was purified again using spin columns ...
-
bioRxiv - Plant Biology 2021Quote: ... and expression plasmid carrying AHK5HK-RD gene into ER2566 (NEB) strain ...
-
bioRxiv - Neuroscience 2022Quote: ... the template plasmid was linearized with Spe1 (New England Biolabs) and transcribed with T7 polymerase (Roche) ...
-
bioRxiv - Genetics 2022Quote: ... We digested each TFT plasmid with SacI and BglII (NEB) to obtain a linear DNA transformation fragment (Supplementary Figure 10) ...