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Citations for New England Biolabs :
301 - 350 of 789 citations for Mouse Anti Cytomegalovirus Glycoprotein H gH 0861 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... RNA-DNA hybrids were removed with 2 U of RNase H (NEB, M0297S). The synthesized DNA was diluted 1:4 and 1 μl was used for qRT-PCR ...
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bioRxiv - Immunology 2020Quote: CAR-encoding mRNA was transcribed from linearized plasmids encoding anti-human CD19 CAR61 and anti-mouse CD19 CAR71 using T7 RNA polymerase (HiScribe™ T7 High Yield RNA Synthesis Kit, New England Biolabs). The mRNAs were transcribed to contain the 5′ UTR derived from the human a-globin 5′ leader RNA ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed three times with a PBS buffer 1X and then incubated with mouse anti-MBP (NEB, Cat# E8032L) or rabbit anti-V5 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... 8 μg of DNA were treated with 30 U of RNase H (NEB, M0297) for 16 h at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Second strand synthesis was conducted using dUTP instead of dTTP and RNase H (NEB), E ...
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bioRxiv - Plant Biology 2020Quote: ... 19 μL binding mixture containing 4 μL nuclear extracts and 5U Rnase H (NEB) or bovine pancreatic RNase A (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... RNA was then removed by incubation with 1 µl of RNase H (NEB, USA) for 20 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Take half of the genomic DNA (gDNA) for RNase H (New England Biolabs, M0297) treatment at 5 μl per 30 μg gDNA in 200 μl final volume for 24 h ...
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bioRxiv - Genetics 2019Quote: ... for 9 h and treated with 2 μl of calf intestinal alkaline phosphatase (NEB) at 37 °C for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... Incubation for 1 h at 55°C with 8U of proteinase K (NEB P8107S) finally disrupted viral capsids ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... these were incubated in the presence and absence of endoglycosidase H (New England Biolabs) (16 = Asjad et al. ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2-bound proteins were eluted by adding 7.5 μl RNase H (NEB), 2 μl TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... N-linked glycans were removed using endoglycosidase H (Endo Hf, New England BioLabs, P0703). De-glycosylated VISTA protein was separated from Endo Hf via additional nickel affinity chromatography ...
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bioRxiv - Evolutionary Biology 2021Quote: ... mRNA was removed after first strand DNA synthesized with RNase H (New England BioLabs). Combinations of indexed Ion Torrent primers were used to amplify barcodes in the library for each time-point (Figure S5 D ...
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bioRxiv - Immunology 2021Quote: ... Digestion was performed for 1 h using Cut Smart Buffer (NEB, Cat no. B7204S). Next ...
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bioRxiv - Genomics 2020Quote: ... cDNA was mixed with of 0.5 μl RNase H (5U/μl) (New England BioLabs) and 2 μl DNA Polymerase I (10U/μl ...
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bioRxiv - Physiology 2022Quote: ... An RNA digestion step was added using the enzyme RNAse H (NEB, Ipswich, USA) according to the protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB, 1 h 50° C) into backbones previously digested with corresponding restriction enzymes (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then either left untreated or treated with Endo H (New England Biolabs) or PNGase F (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... Fragmented gDNA (4 μg) was digested with 20 U of RNase H (NEB #M0297S) for 6 hours to serve as a negative control ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... Half of the fragmented nucleic acids were digested with RNase H (New England Biolabs) overnight at 37°C to serve as a negative control ...
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bioRxiv - Microbiology 2019Quote: PCR mixtures were digested for 1 h at 37 °C with DpnI (New England Biolabs) to remove the template plasmids ...
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bioRxiv - Microbiology 2021Quote: ... RNA was then removed from the mixture by incubation with 5U RNase H (NEB, USA) and 10μg DNase-free RNase A (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: Treatment with endoglycosidase H was performed as described by the manufacturer (New England Biolabs; P0702S). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... or Endoglycosidase H (translation products of ∼50-150 kDa protein substrates; New England Biolabs, P0702S). All samples were solubilised for 12 h at 37°C and then sonicated prior to resolution by SDS-PAGE (10% or 16% PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... Initial protein-level deglycosylation was performed using 250 U of Endo H (New England Biolabs) for up to 5 μg trimer ...
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bioRxiv - Biochemistry 2022Quote: ... Mif2 was treated for 2 h at 30 °C with lambda-phosphatase (New England Biolabs) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2019Quote: RNase-treated transcription reactions involved incubation with 2.5 units of RNase H (New England Biolabs), 2.5 units of RNase I (Promega) ...
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bioRxiv - Genetics 2020Quote: ... One aliquot of the digested DNA was subjected to overnight RNase H (NEB cat#M0297) treatment at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5 μg of R-loop-containing plasmid was incubated with 0.5 U RNase H (NEB) in 75 mM KCl / 50 mM Tris-HCl pH 7.5 / 3 mM MgCl2 / 10 mM DTT in a 20 μL reaction volume and incubated at 37 °C for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... for 1 h followed by incubating with 2 μL Proteinase K (New England Biolabs, USA) at 65 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Treatment with endoglycosidase H was performed as described by the manufacturer (New England Biolabs; P0702S). Briefly ...
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bioRxiv - Genomics 2019Quote: ... using an oligo(dT)24 primer and then treated with RNase H (New England Biolabs). For each time point ...
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bioRxiv - Molecular Biology 2021Quote: ... reaction was subjected to a 2.5-h digestion with DpnI (New England Biolabs, Ipswich, MA) at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Four ug of fragmented gDNA was digested with 20 U of RNase H (NEB #M0297S) at 37°C for 6 hours to serve as a negative control ...
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bioRxiv - Biochemistry 2023Quote: ... Initial protein-level deglycosylation was performed using 250 U of Endo H (New England Biolabs) for 5 µg trimer ...
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bioRxiv - Microbiology 2020Quote: ... with rotation for 1 hour at room temperature followed by further incubation for 1 hour with the addition of 15 μl of goat anti-mouse IgG magnetic beads (New England Biolabs, Ipswich, MA, USA). The magnetic beads in the supernatant were collected on a magnetic stand and washed 2 times with PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse α-MBP (NEB, 1:10000); mouse α-Pol II CTD clone 8WG16 (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... Filtered (0.45 μm) supernatants were treated with 1 U/ml DNAse I (NEB; 1 h, RT) and purified through a 20% sucrose cushion (2 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 6μg of sonicated DNA was pretreated with 12 μl of RNase H (5000 U/mL; NEB) for incubation at 37°C for 6h prior to immunoprecipitation by S9.6 antibody ...
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bioRxiv - Cell Biology 2021Quote: ... For each sample 250 ng of DNA was digested with 5 Units of RNase H (NEB) as control ...
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bioRxiv - Physiology 2022Quote: ... and the DNA was digested for 1 h with I-SceI (New England BioLabs, Beverly, MA) at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... 10 ul of diluted DNA were used for McrBC digestion (NEB, 4 h at 37 °C) or mock digestion (the same volume of H2O instead of McrBC enzyme was added with all other components the same in the reaction ...
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bioRxiv - Microbiology 2023Quote: ... Filtered (0.45 µm) supernatants were treated with 1 U/ml DNAse I (NEB; 1 h, RT) and purified through a 20% sucrose cushion (2 h ...
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bioRxiv - Cell Biology 2023Quote: ... In some cases immunoprecipitated proteins were treated with PNGase-F or Endo-H (New England Biolabs) according to the manufacturers protocols ...
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bioRxiv - Cell Biology 2023Quote: ... or for 1 h in serum-free DMEM with a cocktail of heparinase I (NEB, P0735S), heparinase II (NEB ...
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bioRxiv - Genomics 2024Quote: ... cells were washed three times with PBS-T and incubated with a gap-fill Phusion mix (1x Ampligase Buffer, 0.4 U/mL RNase H, 100 nM padlock probe oPD_CROPseq_iT7, 0.0125 U/mL NEB Phusion polymerase ...
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bioRxiv - Genomics 2024Quote: ... followed by proteinase K treatment (New England Biolabs, #8102S 20µg/µL, 1.5 h at 55°C). DNA was eluted in Milli Q water ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was collected at 9 h post-IFN treatment using Monarch Total RNA Miniprep Kits (NEB). RNA was prepped for RNA sequencing submission using the NEBNext Poly(A ...