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Citations for New England Biolabs :
301 - 350 of 5309 citations for 6H dibenz C E 1 2 oxaphosphorin 6 oxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 20 of 2 U µL-1 Phusion High-Fidelity DNA Polymerase (New England Biolabs), 160 µL of 40 U µL-1 Taq DNA ligase (New England Biolabs ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... GST-GGA3 was grown overnight in 50 mL of Laria-Bertani broth in NiCo21 E Coli (NEB). The following day the overnight culture was transferred to 1L of terrific buffer ...
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bioRxiv - Microbiology 2022Quote: ... and dam−/dcm− (dam−/dcm− competent E. coli, #C2925) strains were purchased from NEB (Ipswich, MA, USA) and cultured in Luria-Bertani (LB ...
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bioRxiv - Developmental Biology 2020Quote: ... gRNAs were cloned into the digested U6.3>gRNA.f+e vector using T4 DNA Ligase (New England Biolabs) at room temperature for 20 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... XL-10 Gold competent Escherichia coli (E. coli) cells and SOC Outgrowth Medium were purchased from NEB. ATP ...
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bioRxiv - Neuroscience 2023Quote: ... A synthetic DNA block (IDT) coding for the scrambled E domain was subcloned using Gibson-cloning (NEB). GST-α-syn 96-140 Scr and GST-α-syn 96-110 Scr plasmids were synthesized by GenScript (Piscataway ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were ligated to barcode primers 1-20 of NEBNext Index Primers for Illumina sets 1 and 2 (New England Biolabs) and libraries analysed using DNA High Sensitivity chip on an Agilent 2100 Bioanalyzer before being pooled ...
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bioRxiv - Biophysics 2023Quote: ... and a reverse elongation primer (Supplementary Tables 1 and 2) and incubating for 1 cycle of annealing/extension with Q5 polymerase (New England Biolabs). dsDNA product was then incubated with ExoSAP-IT (Applied Biosystem ...
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bioRxiv - Bioengineering 2024Quote: ... containing 0.06 % pluronic acid (F-127, final concentration) and 1 µL of each disulfide bond enhancer 1 and 2 (NEB #E6820). We used droplet oil consisting of 3M™ Novec™ HFE7500 Engineered Fluid (3M ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA synthesis was performed for 1 h at 42°C using M-MuLV reverse transcriptase (200 U, NEB) followed by a heat-inactivation at 80°C for 5 min ...
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bioRxiv - Microbiology 2019Quote: ... and the mixture incubated at 37°C for 1 h prior to the transformation into XL10Gold cells (NEB) (Table S8) ...
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bioRxiv - Cell Biology 2020Quote: ... The extract was incubated for 1 h at 4 °C with amylose resin (New England Biolabs, Ipswich, MA), loaded onto a column ...
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bioRxiv - Biophysics 2020Quote: ... followed by treatment for 1 h at 37 °C with 2.5 units of RNase-free DNase I (NEB). The sample was once again cleaned with RNeasy MinElute Cleanup Kit before applying on a 1% agarose gel for gel extraction and purification with QIAGEN gel extraction kit and elution with RNase free H2O ...
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bioRxiv - Microbiology 2021Quote: ... 100ng of vector was digested for 1 hour at 37°C with 10 U of BamHI-HF (NEB) to create a single double-stranded cut or 10 U Nb.BtsI (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... The unbound fraction was then incubated for 1 hour at 4 °C with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2020Quote: ... Around 500ng of RNA were ligated overnight at 16°C using 20units of T4 RNA ligase 1 (NEB) in the presence of 10% PEG 8000 to favour intramolecular ligations ...
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bioRxiv - Molecular Biology 2020Quote: ... Around 500ng of RNA were ligated overnight at 16°C using 20units of T4 RNA ligase 1 (NEB) in the presence of 10% PEG 8000 to favour intramolecular ligations ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The reaction was incubated for 1 hour at 37 °C and stopped by adding 6X loading dye (NEB) with EDTA and run 2% agarose gel ...
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bioRxiv - Molecular Biology 2019Quote: ... Annealed primers were extended at 60°C using 0.08 U μl−1 Bst 2.0 WarmStart DNA polymerase (New England Biolabs) and 0.8 mM dNTPs in isothermal amplification buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting Gβ1γ2 was then incubated for 1 hour at 4 °C with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... The digestion product was then treated with Antarctic phosphatase for 1 hour at 37°C (New England Biolabs). PCR products were purified using the Zymogen Clean and Concentrator PCR-cleanup kit (Zymo Research ...
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bioRxiv - Neuroscience 2022Quote: ... PCR product was then restriction enzyme digested at 37° C for 1 hour with MseI (New England Biolabs) and then run on a 1.5% Agarose gel ...
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bioRxiv - Genetics 2023Quote: ... Gibson assembly was performed for 1 hour at 50°C using Gibson Assembly Master Mix (New England Biolabs), 500 ng backbone ...
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bioRxiv - Immunology 2023Quote: ... for 1 hour at 37°C and ligation was performed using the T4 RNA ligase I (NEB M0204) for 2 hours at 25°C ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibody rabbit anti-c-Fos (1:3000, New England Biolabs cat. No. 2250S) for 24 hours at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was digested for 1 h at 37°C with PNGase F (New England Biolabs, P0704) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of DNA was incubated 20min at 37°C with 15 units of RNAseH1 (New England Biolabs) and first digested for 2h at 37°C with 35 units of AsiSI restriction enzyme (New England Biolabs) ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μL of 2% SDS and 2 μL of proteinase K (New England Biolabs) were added and the solution was incubated at 37°C for 1 hour before purification with the Qiagen DNA clean up kit ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 μl 10X NEBuffer 2 (New England Biolabs) and nuclease-free water (total of 20 μl ...
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bioRxiv - Biochemistry 2020Quote: ... pET-SUMO and pMALX-E were digested with Nde1/BamHI or Nhe1/ HindIII (New England Biolabs, Frankfurt, Germany), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... E-Syt3ΔC2C was produced by introducing a 2021_2257 deletion using the Q5 site-directed mutagenesis kit (NEN Biolabs). The deletion created a reading frameshift and a premature stop that removed the C2C domain and created a 707 aa long protein with a 35 aa C-neoextension ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic sequence of isolated clones at the target locus was further confirmed by PCR cloning (E#1202S, NEB) and Sanger sequencing (Australian Genome Research Facility ...
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bioRxiv - Microbiology 2023Quote: ... E protein glycosylation status was determined by treating RVP lysates with PNGase F (Cat#P0704S, New England Biolabs) for 3 hours at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Beads were resuspended in 1 volume of buffer A / 300 mM NaCl / 2 mM MnCl2 / 1 mM DTT and incubated with λ protein phosphatase (NEB) at 50 U / mL for 1 hour at 23°C with agitation ...
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bioRxiv - Systems Biology 2019Quote: ... the Nickel-NTA beads were incubated in 80 μl 3’-linker ligation mix with (1 X PNK buffer, 1 µM 3’-adapter, 10% PEG8000, 30U Truncated T4 RNA ligase 2 K227Q (NEB, M0351L), 60U RNasin) ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RT-PCR or qPCR was performed with 1 μl of 1:2 diluted cDNA using the Q5®Hot Start High-Fidelity 2X Master Mix (NEB) or the SYBR™ Green PCR Master Mix (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 µL of 20 mg/mL BSA (New England Biolabs) and 3.5 µL of 5 Weiss U/µL T4 DNA Ligase (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was applied to 6 ml amylose resin (NEB) at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... and 6 U SalI (New England Biolabs, Cat. No. R0138S)) were incubated with the following condition ...
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bioRxiv - Genomics 2019Quote: ... NEBNext Multiplex Oligos for Illumina (NEB, Set 1; cat. # E7335, NEB, Set 2; cat. # E7500) were used for multiplexing ...
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bioRxiv - Biochemistry 2020Quote: ... pre-tRNA stock was diluted 1 in 2 into RNA loading buffer (New England Biolabs) and separated on a 10% acrylamide urea-TBE denaturing gel ...
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bioRxiv - Synthetic Biology 2019Quote: ... 5 μl of the resulting amplicon were diluted 1:4 in 1x buffer 2 (NEB), followed by denaturation and re-annealing in a nexus GSX1 Mastercycler (Eppendorf ...
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bioRxiv - Cancer Biology 2021Quote: ... for 1 h followed by incubating with 2 μL Proteinase K (New England Biolabs, USA) at 65 °C ...
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bioRxiv - Genomics 2022Quote: ... and NEBNext Multiplex Oligos for Illumina Index Primers Sets 1 and 2 (NEB E7335S & E7500S). In order to increase input DNA above single library limits (1 μg ...
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bioRxiv - Neuroscience 2023Quote: ... PCR amplified Domain 1 and PCR Domain 2 and HiFi DNA assmbley was performed (NEB). Transformation was performed in DH5α cells (REF) ...
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bioRxiv - Immunology 2021Quote: ... at 25 °C (Creative-Biolabs Inc.). RNA-1 conjugated with biotin (synthesized by IDT lnc. ...
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bioRxiv - Molecular Biology 2022Quote: ... LAMIN A/C – Nucleus (NEB #4777), SDHA – Mitochondria/Mitoplast (NEB #11998) ...
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bioRxiv - Plant Biology 2023Quote: ... and endoproteinase Lys-C (NEB-P8109S) at a ratio of (1:25 ...
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bioRxiv - Bioengineering 2023Quote: ... lysine-C (New England BioLabs, P8109S), protease and phosphatase inhibitor cocktail (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 70 mM Tris-Hydrochloride (Tris-HCl, pH 7.6 at 25°C) and 1 U/µl T4 PNK (New England BioLabs). Reaction mixtures were incubated at 37°C for 30 min ...