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Citations for New England Biolabs :
3351 - 3400 of 9461 citations for Human Malonyl CoA Decarboxylase Mitochondrial MLYCD ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... First strand cDNA was synthesized using a Lunascript® RT SuperMix Kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was further processed using the Monarch RNA cleanup Kit (New England Biolabs, Ipswich, MA). Protocol steps were executed according to manufacturer’s instructions with slight modifications and the RNA was finally eluted in a total volume of 40ul of RNase/DNase free water ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were then PCR purified using a Monarch® PCR & DNA cleanup kit (NEB-T1030S) once a band of the correct size was visualized on a 1% Agarose gel ...
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bioRxiv - Biochemistry 2024Quote: All protein mutagenesis was performed with the Q5 site-directed mutagenesis kit from NEB (E0554). Primers were designed with the NEBaseChanger tool (https://nebasechanger.neb.com/ ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted with the NEBNext rRNA depletion kit for bacteria (New England Biolabs) and a set of 20 pooled sequence-specific probes for Bc ...
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bioRxiv - Biochemistry 2024Quote: ... Libraries were then prepared on beads using NEBNext Ultra II DNA Library Prep kit (NEB) following manufacturers recommendations with 0.6 μM of KAPA Unique Dual-Indexed adapters (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... The crude RNA was preliminarily purified using Monarch RNA Clean-up kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: In Vitro Translation (IVT) was performed using the PURExpress in vitro protein synthesis kit (NEB). Each IVT reaction contained 4 μL of PURE Solution A ...
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bioRxiv - Plant Biology 2024Quote: ... The concentration was estimated using the NEBNext Library Quant Kit for Illumina (New England Biolabs). Each 10 nM library pool (mim-tRNAseq or QuantM-tRNAseq ...
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bioRxiv - Synthetic Biology 2024Quote: RT-qPCR was conducted using Luna®Universal One-Step RT-qPCR Kit (NEB E3005L) following the manufacturer’s documentation ...
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bioRxiv - Genetics 2024Quote: ... PCR fragments were purified using the Monarch DNA Gel Extraction Kit (New England Biolabs, T1020) and assemblies were performed using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using the Monarch Genomic DNA Purification Kit (New England Biolabs, Whitby, Canada) according to the manufacturer’s recommendations for DNA purification from gram-positive bacteria and Archaea ...
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bioRxiv - Synthetic Biology 2024Quote: mRNA was isolated from total RNA extracts using the magnetic mRNA Isolation Kit (NEB S1550S) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Golden Gate Assembly Kit (BsaI-HF v2, E1601L) and the Gibson Assembly Protocol (NEB E5510) were employed ...
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bioRxiv - Synthetic Biology 2024Quote: RNA was extracted 48h after transfection using the Monarch total RNA miniprep kit (NEB T2010S) and stored at −80C until further processing ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was generated from 1 µg RNA using LunaScript RT SuperMix Kit (New England Biolabs). qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted using the NEBNext rRNA Depletion Kit for bacteria (New England Biolabs), cDNA libraries were constructed using the NEB Ultra Directional RNA library prep kit for Illumina and sequenced on Illumina’s NextSeq 1000 platform at 2 × 100 bp read length ...
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bioRxiv - Microbiology 2024Quote: ... The in vitro translation assay was performed using PURExpress In Vitro Protein Synthesis Kit (NEB). 24 μM of antibiotics were added individually to the translation mixture and incubated at 37 °C for 4 hours ...
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bioRxiv - Biochemistry 2024Quote: ... The W75K substitution was introduced using Q5 site-directed mutagenesis kit (New England Biolabs, NEB). Substitutions L80E ...
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bioRxiv - Biochemistry 2024Quote: ... The W75K substitution was introduced using Q5 site-directed mutagenesis kit (New England Biolabs, NEB). Substitutions L80E ...
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bioRxiv - Neuroscience 2024Quote: ... The NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, MA, USA) was performed on the RNA ...
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bioRxiv - Genomics 2024Quote: ... The pooled DNA was PCR-amplified using Phusion® High-Fidelity PCR Kit (NEB®), followed by purification with a Monarch® PCR & DNA Cleanup Kit (NEB®) ...
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bioRxiv - Molecular Biology 2024Quote: ... Y511A mutation in pBAD-His-BrxX was introduced using Q5 site-directed mutagenesis kit (NEB), while introduction of Y511A to the pBREX AL was carried by joining 2 plasmid fragments ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified NbPRS4 PCR product was gel purified (Monarch DNA Gel Extraction Kit, New England Biolabs), digested with XbaI and XhoI (New England Biolabs) ...
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bioRxiv - Biophysics 2020Quote: ... The insert was further cloned into pcDNA3.4 using the HiFi DNA Assembly Kit (New England Biolabs) and PCR products (Phusion polymerase ...
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bioRxiv - Cancer Biology 2021Quote: ... GSE1 coding sequence was modified using the Q5 Site-directed Mutagenesis Kit (E0554S, New England Biolabs) to substitute the cytosine (C ...
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bioRxiv - Cancer Biology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNALibrary Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was synthesized from 700ng of amplified oligos using T7 RNA polymerase (HiScribe T7 kit, NEB) following manufactural instruction ...
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bioRxiv - Cell Biology 2019Quote: ... A point mutation (D697A) was introduced with the Q5® Site-Directed Mutagenesis Kit (E0554S NEB). Yeasts were cultured to an OD600 of 4 ...
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bioRxiv - Cell Biology 2020Quote: The PAQR-2p∷PAQR-1∷GFP construct was generated with a Gibson assembly cloning kit (NEB) with the following three fragments ...
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bioRxiv - Cell Biology 2019Quote: ... 1μg total RNA was used to synthesise cDNA with the ProtoScript II kit (New England Biolabs) using random primers in a 20μl reaction ...
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bioRxiv - Cell Biology 2020Quote: ... The sgRNAs were in vitro synthesized with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). Transcription reactions were treated with RNase-free DNaseI (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... and circularized by an intramolecular ligation reaction using the Quick Ligation™ Kit (New England Biolabs)
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bioRxiv - Immunology 2021Quote: ... the mRNA was synthesized following the HiScribeTM T7 ARCA mRNA Kit (with tailing) (New England Biolabs). Once the mRNA was synthesized ...
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bioRxiv - Genetics 2019Quote: ... Libraries for sequencing were prepped using an NEBNext Ultra II Library prep kit for Illumina (NEB) and loaded for paired-end sequencing using the Illumina HiSeq 1500.
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bioRxiv - Immunology 2021Quote: ... Site-directed mutagenesis was performed using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs). Constructs were verified by Sanger DNA Sequencing at the London Regional Genomics Center ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs). Alternative splicing analysis of RNA sequencing data was completed using the Vertebrate Alternative Splicing and Transcript Tools (VAST-TOOLS ...
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bioRxiv - Molecular Biology 2021Quote: ... The final pool was quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, E7630L) and paired-end sequenced on an Illumina MiSeq.
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bioRxiv - Developmental Biology 2021Quote: The resulting PCR fragments were gel purified using the Monarch Gel Purification Kit (New England Biolabs) and TA cloned into pGEM-T Easy (Promega ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Illumina library preparation was performed with this pool using the NEBNext Ultra kit (NEB, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA sequencing libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB), and sequenced on an Illumina Novaseq PE150 platform ...
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bioRxiv - Microbiology 2021Quote: ... SMU_313 with mutated SmsR4-binding site was constructed using the Q5 Mutagenesis Kit (New England Biolabs) and mutations were confirmed through Sanger sequencing.
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were in vitro transcribed using the HiScribe T7 Quick High Yield RNA synthesis kit (NEB) using 75 ng of template ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by mutation of the MAO binding site using the Q5 site directed mutagenesis kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The cleaved DNAs were subjected to A-tailing using NEBNext Ultra II End Prep kit (NEB) and followed by ligation of an annealed hairpin adaptor (5’-phos-CGGTGGACCGATGATC/ideoxyU/ATCGGTCCACCG*T-3’ ...
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bioRxiv - Genetics 2022Quote: ... Sequencing libraries were prepared using the NEBNext Ultra™ II PCR-free kit (New England Biolabs). In short ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized using a ProtoScript First Strand cDNA synthesis kit (New England Biolabs, MA, E6300S) using PolyDT primers ...
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bioRxiv - Physiology 2022Quote: ... All mutagenesis was done using the Q5® Site-Directed Mutagenesis Kit from NEB (Cat # E0554S). The sequence of oligonucleotides used for this mutagenesis reaction is available on request ...
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bioRxiv - Molecular Biology 2022Quote: ... gel purified and in vitro transcribed using the HiScribe T7 high yield RNA synthesis kit (NEB) in a 20 μl reaction for 10 h at 37°C ...