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Citations for New England Biolabs :
3251 - 3300 of 4045 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... The DNA were digested with 10 units of the appropriate restriction enzymes (New England Biolabs) for 1 h at 37°C and loaded on a 1% agarose gel.
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bioRxiv - Cell Biology 2019Quote: ... Approximately 10 μg of gDNA was digested with StuI-HF restriction enzyme (Cat# R0187M, NEB). Genomic fragments were then resolved on a 0.7% agarose gel for 4/5 hr at 140V in TBE buffer and transferred overnight from the gel via capillary action to a Zeta-probe membrane (Cat#162-0165 ...
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bioRxiv - Bioengineering 2019Quote: ... 5 μL of 10 × GlycoBuffer 2 (supplied by NEB, 500 mM Sodium Phosphate, pH 7.5), and 1 μL of PNGase ...
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bioRxiv - Microbiology 2019Quote: ... The reaction solution was prepared in the following order: 10 μL solution A (NEB #E6800), 7.5 μL solution B (NEB #E6800) ...
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bioRxiv - Genomics 2019Quote: ... and 10 µL of 5 U/µL DNA Polymerase I Large (Klenow) Fragment (NEB, M0210). The reactions were then rotated at 37°C for 45 min ...
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bioRxiv - Microbiology 2019Quote: ... 5 μg DNA samples were digested with 10 u HindIII (New England Biolabs, Ipswich, MA) for 2 h at 37°C ...
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bioRxiv - Genetics 2021Quote: ... were added in a total volume of 10 μl in 1X T4 PNK buffer (NEB) and the mixture was incubated for 1 hr at 44°C ...
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bioRxiv - Genetics 2021Quote: ... 1 μl of 10 mM dNTPs and 0.5 μl of OneTaq Polymerase (New England BioLabs). To generate (partially ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg standard glycoprotein was mixed in 1 μL of 10× glycoprotein denaturing buffer (NEB) (final conc ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 μL of 10 mg/mL Proteinase K (New England Biolabs, Ipswich, MA, Cat # P8107S) and 2.5 μL of 10 mg/mL RNase A (Thermo-Fisher ...
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bioRxiv - Microbiology 2019Quote: ... Intra-molecular reclosure was performed as described (10) using Q5 High-Fidelity DNA Polymerase (NEB). QuickChange (Agilent ...
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bioRxiv - Biophysics 2020Quote: ... and labeled with 10 μM SNAP-Cell® TMR-Star (New England Biolabs Inc. #S9105S) at room temperature for 10 minutes ...
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bioRxiv - Genomics 2019Quote: ... 10 uM RNPs were mixed with 62 uM HiFiCas9 (IDT) and 1x CutSMart buffer (NEB) and incubated at RT for 15 minutes produce Cas9 RNPs ...
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bioRxiv - Cell Biology 2022Quote: ... in the presence of a final concentration of 10% PEG-8000 (New England Biolabs, B1004), in a total volume of 12 μL ...
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bioRxiv - Molecular Biology 2022Quote: ... 1µg of total DNA was treated with 10 U of Topoisomerase I (New England Biolabs) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were recovered in 1 ml NEB® 10-beta/Stable Outgrowth Medium (NEB, B9035) at 37 °C for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... 10 nM SNAP sample was mixed with 3.6 U terminal transferase enzyme (NEB, Ipswich, MA) and 1.32 mM dTTP (NEB ...
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bioRxiv - Genomics 2019Quote: ... the isolated DNA was reverse-crosslinked (adding 10 mg/ml proteinase K (NEB, Ipswich, America) and 1% SDS (AMBION ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in 100 μl CIP buffer with 10 units of calf intestinal alkaline phosphatase (NEB) and incubated for 30 min at 37°C in a heat block ...
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bioRxiv - Microbiology 2020Quote: ... 1x Isothermal Amplification Buffer II (NEB, 20 mM Tris-HCl pH 8.8, 10 mM (NH4)2SO4 ...
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bioRxiv - Bioengineering 2019Quote: ... LAMP mix contained 10 µL 2×WarmStart LAMP Mastermix (New England Biolabs, Ipswich, MA, USA) and 6 µL water ...
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bioRxiv - Biochemistry 2021Quote: ... Then 10 μM of each annealed oligonucleotide and 1μl of 50bp ladder (New England BioLabs) were loaded on a 0.6% agarose gel supplemented with 1X gel red and separated at 100V for 50 min in 1XTBE ...
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bioRxiv - Immunology 2019Quote: ... second-strand synthesis was performed in solid phase in 10 μl using Q5 Polymerase (NEB) and a mix of 13 primers covering IGHV leader sequence ...
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bioRxiv - Microbiology 2021Quote: ... 10 μl NEBNext Quick T4 DNA Ligase NEBNext Quick Ligation Module (New England BioLabs, 6056) and 5 μl Adapter Mix (AMX) ...
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bioRxiv - Genomics 2021Quote: ... consisting of 150 μL 10X NEB T4 DNA ligase buffer with 10 mM ATP (NEB), 125 μL 10% Triton X-100 ...
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bioRxiv - Genetics 2020Quote: ... The 10 μl reactions contained 5 μl Q5 High-Fidelity Master Mix (New England Biolabs), 1 μl forward primer [10 μM] ...
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bioRxiv - Microbiology 2020Quote: ... resuspended in 100 μl CIP buffer with 10 units of calf intestinal alkaline phosphatase (NEB) and incubated for 30 min at 37°C in a heat block ...
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bioRxiv - Genomics 2021Quote: ... and samples were treated with 10 µl of DNase I (New England Biolabs, Ipswich, Massachusetts) for 15 min at 37 °C ...
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bioRxiv - Genetics 2020Quote: ... 10-20 μL of bound DNA was used for library amplification using PCR (NEB, E7370S). Routinely 8-10 PCR cycles were used to generate enough amount of library DNA for sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl 10 mM dNTPs and 1 μl Bst 2 WarmStart DNA polymerase (NEB M0538S) was added and sample incubated 30 min at 65°C before precipitation with 12.5 μl 10 M NH4OAc ...
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bioRxiv - Microbiology 2022Quote: ... Then the tRNA were digested with 10 U Nuclease P1 (New England Biolabs, MA, USA) at 37°C overnight followed by dephosphorylation using 10 U bacteria alkaline phosphatase (Thermofisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... and Phusion DNA polymerase after 20 cycles of amplification (1.2×10−5) (NEB, product literature). Because the error rate from the RNA polymerase was much higher than the RT and PCR steps ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction was initiated by addition of 10 U (pmol/min) of recombinant CK1 (NEB), CDK1-cyclin B1 (NEB) ...
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bioRxiv - Immunology 2022Quote: ... and incubated with a ligation reaction mixture (10 unit/μL T4 DNA ligase (NEB, M0202M), 1x T4 DNA ligase buffer ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was performed with 10 ng of total RNA using OneTaq (New England Biolabs) PCR reaction system with the primers listed in Table S3 ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μM Rad51 and 10 μM (nucleotide) φX174 circular single-stranded DNA (ccsDNA) (N3023L, NEB) were mixed in reaction buffer (25 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... transformations that involved the CRE library were performed using electroporation (NEB 10-beta electrocompetent E.coli), in all other cloning steps chemically competent E ...
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bioRxiv - Biochemistry 2024Quote: ... Purified products were again transformed into either NEB 10-beta (New England Biolabs, Ipswich, MA) or SIG10 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... resuspended in 500 μL 10 mM Tris-HCl buffer and digested with DpnII (NEB, R0543) overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The cosmid-I95 plasmid was amplified in a NEB 10-beta (New England Biolabs, C3019H), and the DNA was purified using a QIAfilter Plasmid Midi Kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluent was subsequently mixed with 10 U of Bst 2.0 WarmStart DNA polymerase (NEB) and 1× Q5 polymerase reaction buffer (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μg of plasmids were then digested overnight with the restriction enzyme CspCI (NEB, #R0645S) in a total volume of 50 μL ...
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bioRxiv - Biochemistry 2023Quote: ... while the other was digested at 25 °C with 10 units SwaI (New England Biolabs). Enzymes were inactivated at 65 °C for 20 min ...
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bioRxiv - Bioengineering 2023Quote: ... Rectangle DNs were folded with 10 nM of M13mp18 ssDNA scaffold (Bayou Biolabs, P-107) and annealed in 20x excess staples ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The 10-µl reactions contained: 5 µl Luna Universal qPCR Master Mix (New England Biolabs), 0.25 µl forward primer (10 µM) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified on a 5-10 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Molecular Biology 2023Quote: ... the ligation reaction was performed by introducing 10 units T4 DNA ligase enzyme (NEB, USA) and incubating at 20 °C for 1 hour to seal the 5’ and 3’ ends of the ssPCRP ...
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bioRxiv - Molecular Biology 2022Quote: ... single cells were lysed in 100 nL lysis mix (10 nL 1X Cutsmart buffer (NEB), 10 nL Proteinase K (Ambion) ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min and treated with PNGase F or Endo Hf enzymes (New England BioLabs) for 1.5 h at 37°C in accordance with the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... the first aliquot was further treated with 10 U of PNGaseF (New England BioLabs, P0705S) for 8 hr at 37 °C.