Labshake search
Citations for New England Biolabs :
3201 - 3250 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The FBXO22-C326A mutant plasmids are generated via Q5 Site-Directed Mutagenesis Kit (NEB E0552) based on the wildtype FBXO22-HaloTag plasmid.
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated using the Monarch® Total RNA Miniprep Kit (#T2010S; New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and cDNA was generated using Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs). SARS-CoV-2 was detected by PCR using the 2019-nCoV RUO Kit (IDTDNA ...
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bioRxiv - Plant Biology 2023Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Mutations on mEGFP-ATP7B were prepared following Q5® Site-Directed Mutagenesis Kit (NEB #E0554) protocol ...
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bioRxiv - Microbiology 2023Quote: ... The 3 fragments were ligated using the Gibson Assembly® Cloning Kit (New England Biolabs). The created pEXG2::ΔfahA and pEXG2::ΔpanC were transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S/L) was used for library preparation and libraries were sequenced on Illumina MiSeq or NextSeq 2000 platform and more than 1.25M read pairs of 150bp length were generated per sample i.e ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... Single point mutant plasmids were constructed using Q5® site-directed mutagenesis kit (NEB, E0554S) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using a NEBNext Ultra II DNA library prep kit (NEB, Cat# E7645S) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using the Monarch Genomic DNA Extraction Kit (New England Biolabs, Massachusetts, USA) before sequencing with MinION (Oxford Nanopore Technology ...
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bioRxiv - Cell Biology 2023Quote: ... and ∼250 bp fragments were gel extracted for cloning using a PCR Cloning Kit (NEB). The resulting plasmid was purified by Midiprep (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... RNAs were transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). Reactions were set up as per manufacturer’s instructions with the addition of 4 units of Murine RNase Inhibitor (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... coli C2566 genomic DNA was purified using Monarch Genomic DNA Purification Kit (NEB Ipswich, MA), GM12878 genomic DNA was obtained from Coriell Cell Repositories (Camden ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared using the NEBNext Ultra DNA Library Prep kit (E7645, New England Biolabs).
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bioRxiv - Biophysics 2024Quote: ... The purified dsDNA templates were transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... with the exception of KLF2 which was performed using the Q5 High-Fidelity kit (NEB). For the 11q deletion variant (P3) ...
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bioRxiv - Biophysics 2024Quote: The NEB Q5® Site-Directed Mutagenesis Kit (part number: E0554S, New England Biolabs Inc.) was used to perform the mutation responsible for mEGFP following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... mRNA was transcribed with HiScribe T7 mRNA Kit with CleanCap Reagent AG (New England Biolabs) according to manufacturer’s protocol with 1 µg of amplified template ...
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bioRxiv - Developmental Biology 2024Quote: ... in combination with NEBNext Ultra II directional RNA library prep kit for Illumina (NEB #E7765) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNA was synthesized from linearized plasmid using HiScribe T7 high yield RNA synthesis kit (NEB), then capped with m7G (5’ ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries for sequencing were constructed with the NEB Next Ultra RNA Library Prep Kit (NEB) for Illumina ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using the Monarch Total RNA Miniprep Kit (New England BioLabs #T2010S) and submitted to Azenta Life Sciences for library preparation and Illumina sequencing.
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bioRxiv - Microbiology 2024Quote: ... quantified the two pools using an NEBNext Library Quant Kit for Illumina (New England Biolabs), and determined average fragment size and adaptor dimer removal on a Tapestation 4200 (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product was purified using the Monarch PCR and DNA cleanup kit (T1030S, NEB) and dsRNA was synthesised using the T7 RiboMAX express system (P1700 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and purified the rest of the digestion product with Monarch Nucleic Acid Purification Kit (NEB). We quantified the amount of purified DNA using the Qubit dsDNA HS Assay (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: ... The DNAse-digested RNA was purified using the Monarch RNA Cleanup kit (New England Biolabs) as recommended by the manufacturer and used as template for cDNA synthesis and SYBR Green-based qPCR (see quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Microbiology 2024Quote: ... Additional mutations/deletions were introduced in gp140-T/F07 using site directed mutagenesis kit (NEB). The sequences of the recombinant clones were verified through sequencing (Retrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplification products were gel extracted using the Monarch® DNA Gel Extraction Kit (NEB T1020).
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were purified using the Monarch DNA PCR Cleanup Kit (New England Biolabs; T1030). For Gibson assembly of constructs ...
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bioRxiv - Cell Biology 2024Quote: ... a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645S) was used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB; E7645) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... following the manufacturer’s guidelines provided with the library kit (E7560S, New England BioLabs Inc., USA). Monarch PCR purification kit (Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were extracted from agarose using a Monarch DNA Gel Extraction Kit (NEB, # T1020), quantitated and next generation sequencing was performed using an Illumina NextSeq 75 cycle High Output kit on an Illumina NextSeq platform.
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bioRxiv - Cancer Biology 2024Quote: ... Fragmented DNA samples was purified using the Monarch DNA Clean up Kit (New England Biolabs). The libraries were prepared from 5-10ng purified CUT&RUN-enriched DNA ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs #T2010S). Reverse transcription was performed using iScript Reverse Transcription Supermix (BioRad #1708841 ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was extracted using Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, T3050), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... First strand cDNA was synthesized using a Lunascript® RT SuperMix Kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was further processed using the Monarch RNA cleanup Kit (New England Biolabs, Ipswich, MA). Protocol steps were executed according to manufacturer’s instructions with slight modifications and the RNA was finally eluted in a total volume of 40ul of RNase/DNase free water ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were then PCR purified using a Monarch® PCR & DNA cleanup kit (NEB-T1030S) once a band of the correct size was visualized on a 1% Agarose gel ...
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bioRxiv - Biochemistry 2024Quote: All protein mutagenesis was performed with the Q5 site-directed mutagenesis kit from NEB (E0554). Primers were designed with the NEBaseChanger tool (https://nebasechanger.neb.com/ ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted with the NEBNext rRNA depletion kit for bacteria (New England Biolabs) and a set of 20 pooled sequence-specific probes for Bc ...
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bioRxiv - Biochemistry 2024Quote: ... Libraries were then prepared on beads using NEBNext Ultra II DNA Library Prep kit (NEB) following manufacturers recommendations with 0.6 μM of KAPA Unique Dual-Indexed adapters (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... The crude RNA was preliminarily purified using Monarch RNA Clean-up kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: In Vitro Translation (IVT) was performed using the PURExpress in vitro protein synthesis kit (NEB). Each IVT reaction contained 4 μL of PURE Solution A ...
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bioRxiv - Plant Biology 2024Quote: ... The concentration was estimated using the NEBNext Library Quant Kit for Illumina (New England Biolabs). Each 10 nM library pool (mim-tRNAseq or QuantM-tRNAseq ...
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bioRxiv - Synthetic Biology 2024Quote: RT-qPCR was conducted using Luna®Universal One-Step RT-qPCR Kit (NEB E3005L) following the manufacturer’s documentation ...
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bioRxiv - Genetics 2024Quote: ... PCR fragments were purified using the Monarch DNA Gel Extraction Kit (New England Biolabs, T1020) and assemblies were performed using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using the Monarch Genomic DNA Purification Kit (New England Biolabs, Whitby, Canada) according to the manufacturer’s recommendations for DNA purification from gram-positive bacteria and Archaea ...
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bioRxiv - Synthetic Biology 2024Quote: mRNA was isolated from total RNA extracts using the magnetic mRNA Isolation Kit (NEB S1550S) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Golden Gate Assembly Kit (BsaI-HF v2, E1601L) and the Gibson Assembly Protocol (NEB E5510) were employed ...