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Citations for New England Biolabs :
3151 - 3200 of 4208 citations for Huntingtin Interacting Protein 1 Related Protein HIP1R Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... PCR product was then restriction enzyme digested at 37° C for 1 hour with MseI (New England Biolabs) and then run on a 1.5% Agarose gel ...
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bioRxiv - Molecular Biology 2023Quote: CDKN1A intron 1 APA 3’ splice site was deleted using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) as per the manufacturer’s instructions using forward primer (5’-TCCCCACCCCAAAATGACGCGCAGCC-3’ ...
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bioRxiv - Microbiology 2023Quote: ... 20 pmol of the obtained RNA were then radiolabeled by incubation with 1 U of Polynucleotide Kinase (NEB) and 20 μCi of 32P-γATP for 1 h at 37°C in a 20 μL reaction volume ...
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bioRxiv - Biophysics 2023Quote: ... and subsequently mixed with ∼0.2 ng of coilable ∼3.3 kbp dsRNA and 1 mg/ml bovine serum albumin (BSA, New England Biolabs). The ∼3.3 kbp length includes only the stem ...
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bioRxiv - Genomics 2023Quote: ... LIDAR-3_RT_antisense was digested by adding 1 μL of USER II enzyme (New England Biolabs, Cat. No M5508S) and incubating at 37°C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... The sequence corresponding to residues 1-239 was amplified by PCR using Q5 High-Fidelity DNA Polymerase (NEB), cut with restriction enzymes NdeI (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... the IP buffer was adjusted to 1% SDS and 0.8 mg Proteinase K (New England Biolabs, Ipswich, MA) was added ...
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bioRxiv - Cancer Biology 2023Quote: We then set up the digestion reaction (1.5 μg of pX459, 2 μL of 10X NEBuffer 2.1, 1 μL of BbsI (NEB), and added H2O to a final volume of 20 μL) ...
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bioRxiv - Cancer Biology 2023Quote: ... and samples were barcoded using the NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB), listed in the Supplementary Data 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Exonuclease I reaction mixture (1X Exo-I buffer, 1 U/µL Exo-I (New England Biolabs, cat# M0293L)) was pipetted into the device followed by an incubation at 37 °C for 45 min ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... resuspended purified nuclei were incubated with 1 mM CaCl2 and 100 ku/ml micrococcal nuclease (New England Biolabs) in HB for 37°C for 15 min ...
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bioRxiv - Biochemistry 2023Quote: ... and transformed 1 µL of the reaction mixture into 6 µL of BL21 competent cells (New England Biolabs). After heat shock and recovery in SOC media ...
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bioRxiv - Physiology 2023Quote: ... The PCR was performed in a total volume of 50 µl containing 1× HF buffer (New England BioLabs), 1 µl dNTP Mix (New England BioLabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg total RNA was reverse transcribed with 50 ng random hexamers using M-MuLV reverse transcriptase (NEB). 1/20th of the resulting cDNA was used then analyzed by qRT-PCR using GoTaq qPCR master mix (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ligation of the RNA 3’ Adapter (RA3) was achieved by using T4 RNA Ligase 1 (NEB, Ipswich, MA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Pellets were resuspended in 99.25 µL micrococcal nuclease reaction buffer (1:10 micrococcal nuclease 10 x buffer (NEB), 1:100 10 mg/mL BSA in distilled water ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM NaCl, 2 mM EDTA, 1% NP40, 0.1%SDS, 0.5 mM DTT, 40 U RNase inhibitor [New England Biolabs, cat#M0314L] ...
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bioRxiv - Synthetic Biology 2023Quote: ... The ligations were transformed into high efficiency (1-3 × 109 CFU/μg pUC19 DNA) competent cells (NEB C3040). The transformations were serially diluted and plated on LB with ampicillin (100 μg/ml) ...
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bioRxiv - Synthetic Biology 2023Quote: ... <1 ng of DNA of interest was pipetted to a mix of 1x rCutsmart buffer (New England Biolabs), 100 μM of primer ...
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bioRxiv - Neuroscience 2023Quote: ... 10µL ligation mix was prepared (20ng digested pX552 vector, 0.8µL 1:50 diluted oligo mix, 5µL 2X T7 Buffer (BioLabs #B0318S), 0.5µL T7 DNA Ligase (BioLabs #M0318S ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... The wash solution was then replaced with 1× T4 polymerase buffer containing 30 U T4 DNA polymerase (NEB) and incubated at 12 °C for 30 min ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... P5 adapters were ligated to the ends with 1 μl of 2000 U/μl T4 DNA Ligase (NEB) at 16 °C for 20 hr ...
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bioRxiv - Genetics 2023Quote: ... Gibson assembly was performed for 1 hour at 50°C using Gibson Assembly Master Mix (New England Biolabs), 500 ng backbone ...
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bioRxiv - Genetics 2023Quote: ... Libraries were indexed with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England BioLabs E7600S). Completed libraries underwent quality assessment on the Agilent Fragment Analyzer run by JH SCTC ...
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bioRxiv - Developmental Biology 2023Quote: ... comparing the intensities of the major dsRNA bands to known amounts of 1 kb DNA ladder marker (NEB) bands using Fiji (Schindelin et al ...
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bioRxiv - Immunology 2023Quote: ... for 1 hour at 37°C and ligation was performed using the T4 RNA ligase I (NEB M0204) for 2 hours at 25°C ...
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bioRxiv - Microbiology 2024Quote: In vitro transcribed CHIKV RNA (nt’s 1-337) was 5’ dephosphorylated using Quick CIP according to the manufacturer’s instructions (NEB) before purification using an RNA Clean & Concentrator column (Zymo Research) ...
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for the PCR were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for the PCR were as follows ...
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bioRxiv - Biochemistry 2024Quote: ... The unbound fraction was then incubated for 1 h at 4 °C with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... in a reaction solution containing 50 mM sodium phosphate (pH 7.5) and 1% NP-40 (B2704S, NE BioLabs) for 1 h at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... along with a well containing 10 µl Quick-Load® Purple 1 kb DNA Ladder (New England Biolabs catalog no ...
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bioRxiv - Cell Biology 2024Quote: ... The medium was then replaced with a fresh imaging medium containing 1 µM SNAP Cell Block (NEB, S9106S) to prevent the binding of residual SNAP dye to newly synthesized SNAP-tagged proteins ...
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bioRxiv - Synthetic Biology 2024Quote: ... Recovery PCR reactions were carried out with 1 µl of the selection product in Taq DNA polymerase (NEB) reactions with recovery primers (CSR_Rec_SHORT_F1/R1 ...
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bioRxiv - Biophysics 2021Quote: ... in 500 μl T4 ligase buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM ATP and 10 mM DTT, pH 7.5, NEB). Before adding the ligase ...
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bioRxiv - Cancer Biology 2021Quote: ... 70 mM Tris-Hydrochloride (Tris-HCl, pH 7.6 at 25°C) and 1 U/µl T4 PNK (New England BioLabs). Reaction mixtures were incubated at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μg protein lysate was mixed with 1 pmol ssDNA substrate (IDT, Germany) and 0.75 U uracil-DNA glycosylase (UDG; New England Biolabs) in a total volume of 12 μL and incubated at 37 °C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 μg/ml phenylmethylsulfonyl fluoride and protease inhibitor) and resuspended again in 1 ml MNase digestion buffer with 1,250 Units MNase (NEB Biolabs). Chromatin-MNase mix was incubated at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... coli CJ236 was picked into 1 ml of 2YT media (Roth, 6676.2) supplemented with M13KO7 helper phage (NEB, N0315) to a final concentration of 1 × 108 pfu/ml and ampicillin (final concentration 100 µg/ml ...
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bioRxiv - Genetics 2021Quote: ... 1μl XhoI digestion mix consisting of 5U XhoI restriction enzyme and 1 x NEB buffer 2.1 (New England Biolabs), and 10 ng genomic DNA for iciHHV-6B samples or 200 ng DNA for non-iciHHV-6B samples ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10 μM of the dsAdR adaptor along with 1 μl (400 U) T4 DNA Ligase (New England Biolabs) were added in each ...
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bioRxiv - Genetics 2021Quote: ... EagI/KpnI-digested ORFs were ligated into the EagI/KpnI-digested pUASTattB vector backbone using a 6:1 insert:vector molar ratio and T4 DNA ligase (M0202S, NEB) in a thermocycler overnight (∼16 hr) ...
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bioRxiv - Developmental Biology 2020Quote: ... 75°C) with 0.5 μg mouse Cot-1 DNA supplemented with 10 mM vanadyl ribonucleoside complex (VRC) (NEB, S1402S) and 0.2 U μL−1 RNAseOUT (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: The rat HA-tagged PRG-1 ORF construct was generated by Gibson Assembly Cloning from NEB (Ipswich, MA, #E5510S) of the Plppr4 ORF clone (NM_001001508.2 ...
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bioRxiv - Molecular Biology 2020Quote: ... The un-crosslinked suspension was treated with 1 μg of RNase A at 37°C for 30 min and with 0.8 units of Proteinase K (NEB) at 45°C for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The plug was equilibrated for 1 h at room temperature in 100 μl 1x Quick Ligation buffer (NEB B6058S) containing 2.7 μl END-seq adaptor 1 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µl 10 x dNTPs with dUTP instead of dTTP (2 mM each) and 1 µl T4 polymerase (NEB) were added and the mixture was incubated at 37°C in a thermal cycler for 20 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and resuspended in 50 µL of 1 mg/mL RNAse A solution (New England Biolabs, Ipswich, Massachusetts, United States) and statically incubated at 37 °C for 3 h ...
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bioRxiv - Genomics 2020Quote: ... were combined with RT primer mix [1 μl 250 ng/μl randomhexRT primer and 0.5 μl 10 mM dNTPs (NEB)] ...
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bioRxiv - Genomics 2019Quote: ... 0.5 ng of genomic DNA was digested with 20 µL of MspI master mix (1 µL of MspI (NEB), 2 µL 10x NEB CutSmart Buffer in a total volume of 20 µL ...