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Citations for New England Biolabs :
3151 - 3200 of 3282 citations for Herpes Simplex Virus 1 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... RNA samples were then processed through the following steps: (i) 3′ adapter ligation with T4 RNA Ligase 1 (NEB, Ipswich, MA; M0204), (ii ...
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bioRxiv - Neuroscience 2022Quote: ... the 181-bp and 376-bp amplicon products (Figure 1-figure supplement 1A) were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) and sent for Sanger DNA sequencing at the OHSU Vollum Sequence Core (Portland ...
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bioRxiv - Molecular Biology 2023Quote: ... The digested T/F LTR U3R region was cloned into the linearized pGL3 Basic vector/plasmid and ligated using 1 U of T4 DNA ligase (New England Biolabs, MA, USA) as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: RE digestion reactions are prepared by adding 1/10th of the final reaction volume of 10x reaction buffer supplied by the RE manufacturer (typically NEB CutSmart buffer) and 0.1% Tween-20 to the DNA solution (diluted to the desired concentration in nanopure DI water) ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was purified from 1 μg of total RNA using an NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA) and following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... the upstream and downstream probes of DLP pairs was ligated by applying a ligation mix containing 1× SplintR buffer (Cat No. B0375S; NEB, Beijing, China), 2.5 U/μL SplintR Ligase (Cat No ...
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bioRxiv - Cell Biology 2022Quote: ... PRR14 1-212 PCR products were sub-cloned into the expression vector pCMV6-AN-mGFP using T7 ligase (New England Biolabs, cat# M0318S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The kinesin and GBP constructs were exchanged into 1x PBS with 1 mM DTT and labeled with DNA and SNAP-Surface Alexa Fluor 647 (NEB, Ipswich, MA) dye directly after elution ...
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bioRxiv - Biochemistry 2024Quote: ... coli (Suppl. Table 1) were cloned into a pBAD24 with ampicillin resistance using the USER cloning method (New England Biolabs, Table 2). All sequences were cloned including a 10x histidine tag located in the N-terminus for BtuG1-3 and the C-terminus for BtuG2 and all its mutants ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was prepared with 1 µg of total RNA using the Protoscript First Strand cDNA Synthesis kit (New England Biolabs; Catalog #E6560L) as instructed by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... at 37° C for 2 hrs from 1 µg purified dsDNAs using a T7 high yield RNA synthesis kit (NEB, Cat # E2040S) in a total volume of 20 µL ...
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bioRxiv - Systems Biology 2024Quote: ... Eluted glycopeptides were lyophilized and then deglycosylated by incubation with 50 µL of 1 unit per microliter of PGNase F (New England Biolabs, Ipswich, MA) in 50 mM Tris-HCl (pH=7.5 ...
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bioRxiv - Immunology 2024Quote: ... The region after the TRR to the middle of exon 1 was amplified from 50 ng of gDNA using Phusion® High-Fidelity polymerase (New England Biolabs, M0530) according to the manufacturer’s instructions with the following primers ...
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bioRxiv - Genomics 2023Quote: ... was extracted from hTERT RPE-1 dry cell pellets using the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs, NEB T3050L) and for tissue (NEB T3060L) ...
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bioRxiv - Genomics 2024Quote: ... RNA from each sample was enriched from 1 µg total RNA by the NEBNext® Poly(A) mRNA Magnetic Isolation Module (Catalog #: E7490, New England Biolabs Inc.). RNA-Seq libraries were prepared using NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (Catalog # ...
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bioRxiv - Microbiology 2024Quote: ... GST-IpaH4-3XFlag was cloned into pGEX6P-1 with a 3XFlag peptide followed by the coding sequence for ubiquitin using Gibson Cloning (NEB; Table S4). Sequence-verified constructs were transformed into E ...
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bioRxiv - Microbiology 2024Quote: ... was reverse transcribed from ∼1 μg high quality RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEW ENGLAND BioLabs, Germany). Newly synthesized cDNA was purified using a the NucleoSpin® Gel and PCR Clean-up kit (Germany ...
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bioRxiv - Neuroscience 2023Quote: ... samples were split into two separate reaction tubes containing 8 μL of PCR product + 1 μL 10X NEB® Buffer #2 (New England Biolabs®). These were incubated in a thermocycler with an initial denaturation of 95 °C for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and lysed with 2× concentrated lysis buffer (0.25% Triton X-100, 50 mM KCL, 100 mM Tris-HCl pH 7.4, 40% glycerol, 1/50 volume of RNase inhibitor; NEB, Cat. No. M0314S) at room temperature for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were propagated in Escherichia coli NEB Turbo ((F’ proA+B+ lacIq ΔlacZM15/fhuA2 Δ(lac-proAB) glnV galK16 galE15 R(zgb-210::Tn10) TetS endA1 thi-1 Δ(hsdS-mcrB)5)) (New England Biolabs) at 37°C in lysogeny broth (LB ...
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bioRxiv - Plant Biology 2023Quote: ... The up- and downstream flanks were obtained by PCR on gDNA of IPO323 with primer pairs 1&2 and 5&6 (Table S7) respectively using Q5 Hot Start High fidelity DNA polymerase (NEB, Evry, France). The hph was amplified from pCAMBIA0380 with the primers 3 and 4 (Table S7) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were resolved on an 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membrane for detection with the following antibodies: anti-Glucocorticoid Receptor D6H2L (1:1000 dilution, Cell Signaling, New England BioLabs #12041), anti-PAX7c (1:500 dilution ...
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bioRxiv - Genetics 2023Quote: ... One microliter of T-tailed DNA adaptors diluted to 1.5 µM in water was added to 5 µl of amplicons diluted to 1/10 in water and 5 µl of 2X Blunt/TA Ligase Master Mix (New England Biolabs, Herts, UK) and incubated 30 min at 25°C for ligation ...
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bioRxiv - Immunology 2023Quote: ... using primers that overlapped with pLenti-EF1-Blast at the xho1 site on the 5’ end and overlapped with the 5’-end of the RNase L coding sequence on the 3’-end (Supplementary table 1) and NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs: M0541S). RNase L coding sequence was amplified using a 3’-end primer that overlapped with pLenti-EF1-Blast at the xba1 site ...
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bioRxiv - Biochemistry 2023Quote: ... in vitro RNA methylation experiments were performed for 2 h at 37 °C in 20 μL reaction mixtures containing 1× CutSmart buffer with 5 μg RNA substrate (1.8 knt. Same substrate of MTase-Glo experiment) and 1 unit RNase Inhibitor (New England Biolabs Inc., Massachusetts, USA), 1 μM methyltransferase or no enzyme as a negative control ...
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bioRxiv - Synthetic Biology 2023Quote: ... The pUdO#a was obtained by assembling four fragments (Table 1 and Figure S1) using the Gibson Assembly® Master Mix (New England Biolabs, E2611) according to the supplier’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... 1 µg of the duplex was in vitro transcribed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB # E2050S) following the standard IVT protocol and purified using Monarch® RNA Cleanup columns (NEB # T2030).
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bioRxiv - Evolutionary Biology 2023Quote: ... we purified the products of the ace-1 PCR (see above) using the BS664-250 Preps EZ-10 Spin Column PCR Purification kit (New England BioLabs, Evry France). The purified PCR products were then cloned (TOPO TA Cloning Kit pCR 2.1-TOPO Vector and TOP10F’ invitrogen bacteria) ...
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bioRxiv - Genetics 2024Quote: ... HMW DNA was also isolated from six PBMC samples (± 1 x 106 cells) using the Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, #T3050L) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Linker ligation at 3’ ends was set up per sample after washes following alkaline phosphatase treatment by preparing a T4 RNA ligase 1 (NEB, cat. #M0204S) reaction in 40μL following the manufacturers’ instructions with 100pmol of radiolabeled L32 RNA linker ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were digested in a 20 μl reaction with NEB Cutsmart buffer for 30 minutes - 1 hour with SmaI (NEB, Cat# R0141) to assess rDNA promoter methylation or with HpaII (NEB ...
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bioRxiv - Cancer Biology 2023Quote: 1 µg total RNA was used for NEBNext® Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA). Poly-A selection and cDNA synthesis were performed according to NEB protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The purified shESR1 and SGEN DNA were digested with XhoI and EcoRI and subsequently ligated at a molar ratio of approximately 3:1 with 10X T4 DNA Ligase Buffer (New England BioLabs Inc. #B0202S) and T4 DNA Ligase (New England BioLabs Inc ...
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bioRxiv - Biophysics 2023Quote: ... For each sample a separate chamber with closed nanosensors was prepared and filled with 1×CutSmart™ buffer (New England BioLabs, USA) containing 50 mM potassium ...
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bioRxiv - Plant Biology 2023Quote: Approximately 1 ug of RNA was used as input to the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs, catalog #E7490S), followed by NEB Ultra II RNA library prep kit for Illumina (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... appropriate number of amplification cycles x (98C - 10sec, 63C – 30sec, 72C – 1 min) with NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) and barcoded Nextera primers (1.25 μM each ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from 1 μg total RNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, catalog #E6560S) with oligo-dT priming according to the manufacturer protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR reactions were run on a 1% agarose gel and positive bands were excised and purified using Monarch Gel extraction clean kit (NEB, Cat. #T1020L). A-tailing of the fragments was carried out by incubating the DNA with MyTaq polymerase (NEB ...
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bioRxiv - Genomics 2024Quote: The combined pool of purified DHS fragments (∼1 μg) was end repaired and ligated to NEBNext adaptor (included in NEB cat. # E7645S) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Gibson assembly was performed by combining a part amplicon and linearized pEmpty in a 2:1 mass ratio in 2× NEB Gibson Assembly Master Mix (NEB, Cat# E2611S) and incubating at 50℃ for 2 hr before transformation ...
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bioRxiv - Biophysics 2024Quote: ... Transcription templates were prepared by PCR using primers complementary to the T7 promoter and terminator sequences (see Supplementary Table 1) or by digestion of the plasmid DNA with SphI (NEB, Cat# R3182S). mRNA fragments were prepared by in vitro transcription with T7 RNA polymerase using a ratio of 10:30 AU:GC NTPs ...
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bioRxiv - Molecular Biology 2024Quote: ... SEGS-1 region N was generated by cleaving the Kpn1 site using the Q5-site directed mutagenesis protocol (New England Biolabs, Ipswich, MA) from S1-1.5a (pNSB1829 ...
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bioRxiv - Molecular Biology 2024Quote: ... and joining SEGS-1F and SEGS-1G using the indicated primers in Supplementary Figure 1 and the Q5 high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA). All the primers except for those for site-directed mutagenesis added NotI restriction sites at the 5’ and 3’ends of PCR products ...
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bioRxiv - Synthetic Biology 2024Quote: The reaction was brought up to 50 uL with the addition of CutSmart (final concentration 1X) and 1 uL DpnI (NEB, Catalog #R0176S) and incubated for 1 hour at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... We isolated poly(A)+ RNA from 1 ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Ipswich, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Briefly 60 ng ±10% of total RNA was subjected to small RNA library preparation by using the NEBNext Multiplex Small RNA Library Prep Set 1 and 2 for Illumina (NEB, Ipswich, MA, USA) kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... followed by the addition of 5 μl of UDG reaction solution (New England Biolabs, 0.02 U μl−1 UDG in 1X UDG buffer) and an additional 30 min incubation at 37 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Detection of editing in the target genes was performed via amplifying the target regions (primers in Supplemental Table 1) with high fidelity DNA polymerase Q5 (New England Biolabs, Ipswich, MA, USA), followed by cloning of PCR products and sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were blocked with 5% BSA in 1X PBS for 30 minutes at room temperature followed by subsequent incubation with primary antibodies against MBP (NEB E8032L, 1:200) and RAD51 (Proteintech 14961-1-AP ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µ l of cfDNA was incubated at 37°C for 1 hour with the following reaction mixture: NEBuffer™ 2 (NEB, B7202), 0.25 mM MnCl2 (SIGMA ...