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Citations for New England Biolabs :
3001 - 3050 of 4892 citations for 6 CHLORO 2 THIOPHEN 2 YL IMIDAZO 1 2 A PYRIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 3.5 µL ddH2O and 1 µL 10 µM RTP primer (NEB) was added ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were incubated with 1 µl Exonuclease I (NEB, #M0293) and 2 µl of 10X reaction buffer at 37°C for 30 min ...
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bioRxiv - Genomics 2023Quote: ... 2.5 μL of Digestion-1 mix (MseI (12.5 U, NEB, R0525M) and/ or MboI (12.5 U ...
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bioRxiv - Cell Biology 2023Quote: ... pAG306GPD-ORF chr 1 was digested with NotI-HF (NEB, #10133990).
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bioRxiv - Systems Biology 2023Quote: ... and 1 μL T4 DNA Ligase (400,000 U/mL, NEB, #M0202S). Ligation occurred at 25°C for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... template DNA was digested by incubation with DpnI (1 μl; NEB) at 37 °C for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... 1 μL of DpnI restriction endonuclease (New England Biolabs; cat # R0176L) was added to each reaction tube to digest DNA templates ...
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bioRxiv - Immunology 2022Quote: ... 1 µl Phusion Hot Start II DNA Polymerase (2U/ µl) (NEB); 1 µl 10 mM dNTPs ...
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bioRxiv - Genetics 2023Quote: ... Then 0.5 µL of 1 MgCl2 and 2µL RNase inhibitor (NEB) were added to each sample and mixed by vortexing ...
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bioRxiv - Immunology 2023Quote: ... 1 mM CaCl2 and treated with N-glycanase (New England Biolabs) for 1 hour at 37°C.
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bioRxiv - Cell Biology 2023Quote: ... cells were stained with 1 µM SNAP-cell SiR-647 (NEB) for 30 min at 37 °C and 5 % CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μl of dNTPs (10 mM of each – New England Biolabs), 1 μl of iTP_Linker_r oligonucleotide (2 μM) ...
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bioRxiv - Biochemistry 2023Quote: ... and probed with either anti-histone H3 (1:1000, NEB, 9715S) or anti-histone H3 (phosphor S10 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 µL of each Exonuclease I (NEB, cat. no. M0293S) and Exonuclease III (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... 72°C for 1 min) using NEBNext 2x Master Mix (NEB) and Nextera adapters and put on ice ...
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bioRxiv - Cell Biology 2023Quote: ... and 200 µg/ml RNAse A (New England Biolabs #T3018-1)) for four hours on ice ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 μl of Lambda protein phosphatase (P0753L, New England BioLabs) were incubated with the proteins at 30°C for 30 minutes.
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bioRxiv - Molecular Biology 2023Quote: ... and 1 U of Taq Standard Polymerase (New England Biolabs #M0273) in a final volume of 30 μL ...
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bioRxiv - Microbiology 2023Quote: ... the elute was recovered by adding 1 μl liner acrylamide (NEB) and precipitating in ethanol at -80°C for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Each 20 µL PCR reaction contained 1× OneTaq Master Mix (NEB), 0.2 µM of each primer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 1 U of Taq DNA polymerase (OneTaq, New England Biolabs). The PCR conditions included an initial denaturation step at 94°C for 3 min ...
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bioRxiv - Cell Biology 2023Quote: ... 1mM DTT) before adding 15 µl 1 mg/ml Streptavidin (NEB) before immediate wash with DLB-C-T buffer (DLB supplemented with 1 mg/ml α-casein ...
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bioRxiv - Molecular Biology 2023Quote: ... or 8 µl RNase III (1:1000 diluted, New England Biolabs) or RNase If (1:100 diluted ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µl of Golden Gate Enzyme Mix (BsaI-HFv2, NEB M2616AA), and adding dH2O to reach a total volume of 20 µl ...
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bioRxiv - Microbiology 2024Quote: ... Ligation of 5’ linker using T4 RNA ligase 1 (NEB; #M0204S) was conducted after phosphorylating the 5’ end of the pooled fragments with T4 PNK (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) (NEB, 7335S) were used for the final PCR amplification ...
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bioRxiv - Microbiology 2024Quote: ... respectively) using T4 RNA Ligase 1 (New England Biolabs, USA; #M0204) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... made up of 1 uL DNA Ladder (New England Biolabs, N3231L), 1 µL Purple Dye (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 72 °C for 1 min) × 5] using NEBNext 2xMasterMix (NEB, M0541S) with Ad1_noMX and v2_Ad2.* indexing primers followed by qPCR amplification to determine additional cycle numbers ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of terminal transferase (20 units, NEB Catalog #M0315S) was added to the reaction with incubation at 37 °C for 1 h to block any pre-existing strand-break sites ...
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bioRxiv - Molecular Biology 2024Quote: ... steps 1–3: GELase was replaced by β-agarase I (NEB). The reaction (1 U per plug ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of 1 μl M13KO7 helper phage (NEB).
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bioRxiv - Microbiology 2024Quote: ... (b) Heat labile Shrimp Alkaline Phosphatase (rSAP) (1 µl) (NEB #M0371S) was used to remove terminal phosphates that could interfere with terminal extension by incubating in rCutSmart buffer (3.5 µl ...
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bioRxiv - Systems Biology 2024Quote: ... IVT products were treated with 1 U of DNase I (NEB) at 37°C for 15 min and brought to a final volume of 100 µL with nuclease-free water ...
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bioRxiv - Genomics 2024Quote: ... 16ul nuclease-free water and 1 μl T4 DNA ligase (NEB). The entire volume of the reaction was pipetted on a parafilm sheet placed on a glass plate and the coverslips were inverted over it ...
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bioRxiv - Genomics 2024Quote: ... Unmethylated λ-bacteriophage DNA (1:200 mass ratio; New England Biolabs) was included in all samples to calculate unmethylated cytosine conversion efficiency ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl (500 Units) of PNGase F (New England BioLabs, Inc.) was used to deglycosylate 10 µg of the protein in the presence of 5 mM DTT and mild detergents by incubating at room temperature for 1 hour according to manufacturer’s recommendations.
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of terminal transferase (20 units, NEB Catalog # M0315S) was added to the reaction with incubation at 37 °C for 1 h to block any pre-existing strand-break sites ...
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bioRxiv - Genetics 2023Quote: ... We added phasing nucleotides as well as overhangs for indexing primers using primer mixtures SL5.F[1-4] and SL5.R[1-4] (NEB Q5 for 20 cycles, Tm 62 °C). We finally added dual indexing primers using the i5 and i7 system from Illumina (NEB Q5 for 20 cycles ...
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bioRxiv - Molecular Biology 2019Quote: ... we supplemented the reaction with 1 U micrococcal nuclease (New England Biolabs) and allowed it to incubate for 10 min ...
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bioRxiv - Genetics 2021Quote: ... 1 ul of 20 μM Cas protein (SpyCas9 and SauCas9 from NEB; Nme2Cas9 was purified as previously described (Edraki et al ...
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bioRxiv - Genetics 2021Quote: ... and the sample was digested with DNase I (NEB [1:20 dilution]) for 30 seconds at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... and 30 μg was diluted 1:3 in gel loading buffer (NEB), sonicated ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by adding 1 μL of proteinase K (800 units/ml) (NEB) and incubating at room temperature for 10 minutes before being run on a 1.7% agarose gel with a 100 bp ladder for 35 minutes.
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bioRxiv - Molecular Biology 2021Quote: ... and together with the addition of apyrase (25 mU mL−1, NEB). No CID2886111 was supplied in the purification ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μl ProtoScript II RT (200 U/μl, New England Biolabs) to a total volume of 20 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 U Q5 hot start high fidelity DNA polymerase (New England Biolabs), 500 nM each primer (Table 2 primers #1-4 for 1116NS19.9 or primers #5-8 for 5b1 ...
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bioRxiv - Molecular Biology 2020Quote: ... 70 μl of end-repair mix was added (1× Ligation buffer (NEB), 357 μM dNTPs ...
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bioRxiv - Genomics 2020Quote: ... Chromatin was digested in situ using restriction enzyme CviKI-1 (NEB, R0710L) and barcoded using RELACS custom barcodes (4bp UMI + 8bp RELACS barcode ...
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bioRxiv - Pathology 2019Quote: ... 1 µl of either SP6 or T7 polymerase (New England Biolabs, USA). SP6 reactions were incubated at 40°C for 2 hours ...