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Citations for New England Biolabs :
251 - 300 of 1956 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... To digest remaining M1 tag forward oligo: 3 µl 20U/ µl Exonuclease I (NEB) was added and samples were incubated for 1 h at 37°C ExoI denaturation was performed for 5 min at 98°C ...
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bioRxiv - Cell Biology 2023Quote: ... To amplify the SNAP tag sequence out of the pSNAPf plasmid (New England Biolabs), the following primers were used ...
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bioRxiv - Cancer Biology 2024Quote: ... CUT&Tag libraries were prepared with NEBNext HiFi 2x PCR Master Mix (NEB M0541S) and indexed primers (Buenrostro et al. ...
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bioRxiv - Immunology 2023Quote: ... Read 1 and Read 2 sequencing primers (sequences listed in Table S2) were added PCR fragments by T/A ligation using NEBNext Ultra II Ligation Module (E7595, New England Biolabs, Ipswich, MA). Spacers and minimal promoter were added to sequencing primer-ligated enhancers using the primers 5BC-AG-f02 and 5BC-AG-r02 (Table S2) ...
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bioRxiv - Microbiology 2022Quote: ... The D614G spike plasmid was generated by introducing the mutation into the Wuhan reference strain via Q5 site-directed mutagenesis (NEB). The T95I ...
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bioRxiv - Microbiology 2022Quote: ... Mutations were introduced into the phCMV-Spike plasmids using the mutagenic primers and the Q5 site directed mutagenesis kit (NEB). In addition ...
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bioRxiv - Biochemistry 2020Quote: ... was replaced with sequence for a 3×FLAG tag by Gibson Assembly (New England Biolabs) to create payload plasmid pSAB41 ...
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bioRxiv - Immunology 2022Quote: ... periplasmic expression vectors with C-terminal HA-His6 tags using Gibson cloning (New England Biolabs). Nanobodies were produced in Escherichia coli WK6 transformed with the respective expression vectors ...
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bioRxiv - Cell Biology 2019Quote: ... Fusions to the human O6-Alkyl-DNA transferase (SNAP-tag, New England Biolabs, Beverly, MA) were expressed from plasmid pAGT-Xpress ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with SNAP-tag ligand SNAP-Cell 647-SiR (New England BioLabs; S9102S) at a final concentration of 100 nM for 15 minutes followed by 30-45 minute washout in i3Neuron Maintenance Media before imaging in i3Neuron Imaging Media ...
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bioRxiv - Plant Biology 2023Quote: AtPAXX fused to a hexahistidine tag (pHAT4-atpaxx) was expressed in BL21(DE3) cells (NEB). The proteins were purified using Ni-NTA (Qiagen) ...
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bioRxiv - Biophysics 2023Quote: ... and BG-oligonuculeotides were labeled with myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer ...
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bioRxiv - Systems Biology 2023Quote: ... Biotinylated oligo tags were amplified on-bead using 2X Q5 Hot-Start Mastermix (NEB #M0494) with primers that add the indexed full Illumina adaptor sequences.
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bioRxiv - Cell Biology 2023Quote: ... and an internal ribosomal entry site driven SNAP-tag (N9181S, New England BioLabs, Ipswich, MA), respectively ...
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bioRxiv - Microbiology 2023Quote: ... His/Strep-Tag was cleaved using enterokinase according to the protocol of the manufacturer (NEB) and GlnA3Mtwas immediately purified from the digestion mix by size-exclusion chromatography as directed by the resin manufacturer (GE-Healthcare).
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bioRxiv - Biochemistry 2024Quote: Constructs bearing a C-terminal cMyc tag were cloned by site-directed mutagenesis (NEB, M0554S) using oligonucleotides as primers bearing a 5’-overhang encoding the inserted sequence ...
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bioRxiv - Biochemistry 2022Quote: ... reactions to generate catalytic arginine point mutants for AIM18 and AIM46 were performed according to the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, Table S2). SDM reactions were transformed into E ...
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bioRxiv - Microbiology 2020Quote: ... THUNDERBIRD Probe qPCR Master Mix (Toyobo) for MERS-CoV and Luna Universal Probe One-Step RT-qPCR Kit (NEB) for SARS-CoV-2.
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bioRxiv - Immunology 2022Quote: ... The spike plasmids were linearized by restriction enzymes and transcribed to mRNA by in vitro T7 RNA polymerase (NEB, Cat # E2060S) as previously described7,8.
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bioRxiv - Immunology 2021Quote: ... The D614G Spike was generated by introducing the corresponding mutation in the Wuhan reference strain using Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... together with an N-terminal FLAG-emGFP-tag via NheI and NotI (both New England Biolabs) restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... the 6xHis-MBP tag was cleaved from 6xHis-MBP-MISP using a TEV protease (NEB; P8112) for 1 hour at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... The human μOR and V2R were amplified with a SNAP tag at their N-terminal (NEB) and subcloned in the pcDNA4/TO plasmid (Invitrogen) ...
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bioRxiv - Plant Biology 2022Quote: ... The HIS tag was removed from the mature sequence of AgLTP24 with enterokinase (NEB, Evry, France) for 16 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The HA protein tag and point mutations were introduced using the KLD Enzyme mix (NEB M0554S) and were verified by Sanger sequencing and whole-plasmid sequencing (Plasmidsaurus).
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bioRxiv - Cell Biology 2023Quote: ... with C-terminal fluorescent tags using seamless cloning (HiFi DNA Assembly Master Mix, New England Biolabs). The pLVX-KRT5-mNG-IRES-Puro construct includes an mNeonGreen (Allele Biotechnology)6 sequence in-frame with the KRT5 sequence separated by a short peptide linker (DPAFLY) ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by simultaneous overnight tag cleavage by TEV and dephosphorylation by λ-protein phosphatase (NEB, P0735). The cleaved ...
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bioRxiv - Biochemistry 2023Quote: ... the Myc/DDK tag was removed via the Q5 site-directed mutagenesis kit (New England Biolabs) using the oligonucleotides mS29 ΔMyc-DDK Forward ...
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bioRxiv - Microbiology 2024Quote: ... Tags (6x His or HA) were introduced using site-directed mutagenesis with KLD enzyme mix (NEB).
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 µl of the dissolved cells were added to a 50 µl PCR reaction (Q5 DNA polymerase, NEB; see Table S2 for primer sequences) and amplified with 36 rounds of thermal cycling ...
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bioRxiv - Genetics 2019Quote: ... The 3′-UTR sequences of both genes were then amplified from genomic DNA obtained from HEK293 cells with Phusion® High-Fidelity DNA Polymerases (NEB, Ipswich, MA) and cloned into the multiple cloning site of the pMIR-REPORT luciferase miRNA expression vector (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μL GlycoBuffer 2 10X (NEB), 2 μL NP-40 ...
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bioRxiv - Biophysics 2022Quote: ... CPB containing a C-terminal His6-tag was generated using Q5® site-directed mutagenesis kit (NEB), pET-19b_His6_CPB as template and primer pair #1 and elongation time of 4 min to delete the N-terminal His6 ...
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bioRxiv - Cell Biology 2019Quote: Cells transfected with corresponding SNAP-tags and stained with silicone-rhodamine dye (SNAP-cell 647-SiR (NEB) (3 µM) ...
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bioRxiv - Cell Biology 2019Quote: Cells transfected with the SNAP-tag and stained with low concentrations of SNAP-cell 647-SiR (NEB) (15 nM for MIC60-SNAP and 0.225 nM for MIC10-SNAP ...
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bioRxiv - Cell Biology 2020Quote: ... a P2A self-cleavage site and a CAAX membrane localization tag were added using Q5 mutagenesis (NEB) with the following primers ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... in frame with the N-terminal His6-tag using the Gibson assembly master mix (New England Biolabs) for TaSS4-1B ...
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bioRxiv - Molecular Biology 2021Quote: ... The His-GST tags were cleaved by incubating the pooled fractions with TEV protease (New England Biolabs) at 4°C for 16h ...
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bioRxiv - Biochemistry 2021Quote: The full-length PARP1 gene was purchased from GE Healthcare and subcloned into a pACEBac1 plasmid bearing an N-terminal 6xHis-tag via a Gibson Assembly (NEB). The PARP2 expression plasmid (C-terminal FLAG-6xHis-tag ...
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bioRxiv - Cell Biology 2021Quote: ... SNAP tag was firstly PCR amplified from pSNAPf Vector (Cat. #N9183S, New England Biolabs, Ipswich, MA, USA) using primers 5’-CGT ACG ATC GAA TTC CCC ATG GAC AAA GAC TGC GAA ATG AAG CGC ACC ACC-3’ (forward ...
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bioRxiv - Bioengineering 2022Quote: ... The t-NLuc-His tag was double-digested with NdeI and XhoI restriction enzymes (New England BioLabs), and the RNA sensor region were digested with SgrAI and SacII restriction enzymes ...
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bioRxiv - Molecular Biology 2022Quote: ... with a cleavable Glutathione-S-Transferase (GST) tag at the N-terminus) and pMALTMc5X (New England Biolabs, USA ...
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bioRxiv - Plant Biology 2022Quote: ... and 3xHA::3C::BLRP tag encoding DNAs were respectively ligated into pENTR/D constructs using AscI (NEB) digestion site in the pENTR/D vector ...
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bioRxiv - Cell Biology 2023Quote: cDNA encoding recombinant MUC17(7TR) with N-terminal 3xFlag tag was generated using Gibson Assembly (E2611S, NEB) following the manufactures protocol ...
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bioRxiv - Biochemistry 2023Quote: ... SUMO21-92 and ISG1579-156 without tag and subsequently cloned into the pTXB1 vector (New England Biolabs) by restriction cloning according to the manufacturers protocol.
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bioRxiv - Molecular Biology 2023Quote: ... Cascade complexes were purified via the N-terminal maltose binding protein (MBP) tag using amylose beads (NEB) and eluted with lysis buffer containing 10 mM maltose ...
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bioRxiv - Microbiology 2023Quote: ... fused to a maltose-binding tag using the pMAL™c5X-vector (New England Biolabs, Ipswich, US). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... A C-terminal HA tag was added using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S). For microinjection ...
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bioRxiv - Biophysics 2024Quote: ... solutions containing polySH3 and cleaved MBP- and His6-tags were passed over Amylose resin (New England Biolabs) to remove excess MBP ...
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bioRxiv - Molecular Biology 2023Quote: ... SNAP-tag coupling activity (methodology described below) was first tested with a small ligand BG-biotin (NEB) or DNA (a 550 bp benzylguanylated PCR product ...