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Citations for New England Biolabs :
251 - 300 of 1167 citations for Recombinant Mouse Angiotensinogen serpin peptidase inhibitor clade A member 8 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM β-ME) supplied with protease inhibitors and an RNase inhibitor (NEB, M0314L) for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and PCR amplified (8-12 cycles) with Phusion polymerase (NEB) using custom primers (22) ...
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bioRxiv - Biochemistry 2019Quote: ... samples were mixed into an 8%-by-volume Dpn1 (NEB) digestion reaction (37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... 8 μL of 5X Quick Ligation Reaction buffer (NEB: B6058S), 6 μL of the RMX adapter and 3 μL of T4 DNA ligase (2,000,000 units/mL).
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bioRxiv - Neuroscience 2020Quote: ... and incubated with 8 μl of T4 Polynucleotide Kinase (NEB) and 2 μl of 10 mM ATP for 30 min at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... 3 mM DTT 8 μl Large Klenow Fragment (NEB, #M0210L) and 2 μl T4 PNK (NEB ...
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bioRxiv - Molecular Biology 2021Quote: 8 different polymerases (KAPA HiFi HotStart, Qiagen Taq, Q5 (NEB), Phusion Hot Start Flex DNA Polymerase (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 8 units of Bst 2.0 warmstart DNA polymerase (NEB) followed by incubation at 60°C for 2 minutes to extend the strand with EdUTP-containing DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... was mixed with 8 mM m7G cap analog (ARCA; NEB), TMG cap analog (Jena Bioscience ...
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bioRxiv - Biophysics 2023Quote: ... 8 mM ATP and 10 units of T4 ligase (NEB) were incubated overnight at 16°C ...
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bioRxiv - Genomics 2023Quote: ... (2) +28 μl Dam at 8 U/μl (NEB M0222L); (3 ...
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bioRxiv - Molecular Biology 2022Quote: ... and phosphatase inhibitors (Sigma, NEB). Immunoblots were performed using standard procedures ...
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bioRxiv - Genomics 2020Quote: ... RNase Inhibitor (1:40, NEB Murine RNAse Inhibitor or Thermofisher Ribolock ...
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bioRxiv - Genomics 2019Quote: ... RNase inhibitor (NEB, 40KU/ml) and Cell Diluent Buffer.
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bioRxiv - Immunology 2021Quote: ... 9 U RNase Inhibitor (NEB) and 90 U Superscript II (Invitrogen) ...
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bioRxiv - Bioengineering 2020Quote: ... and murine RNase inhibitor (NEB) were added at final concentrations of 4 U/µl each ...
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bioRxiv - Systems Biology 2020Quote: ... 1 U RNase Inhibitor (NEB)) ...
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bioRxiv - Cell Biology 2021Quote: ... RNAse inhibitor (NEB cat# MO314L) was added to the homogenization buffer (0.32 M sucrose ...
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bioRxiv - Molecular Biology 2020Quote: ... Murine RNase inhibitor (NEB M3014L) was added at a concentration of 2 units/μl ...
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bioRxiv - Immunology 2020Quote: ... 1 U RNase inhibitor (NEB), 10 mM fresh DTT ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1x RNAse inhibitor Murine (NEB).
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bioRxiv - Immunology 2021Quote: ... 1 U RNase Inhibitor (NEB)) ...
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bioRxiv - Neuroscience 2022Quote: ... 1x RNase Inhibitor Murine (NEB): nuclease-free water (Exiqon ...
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bioRxiv - Molecular Biology 2022Quote: ... Murine RNase inhibitor (M0314L, NEB), Nitrocellulose membrane (10600008 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1U/μl RNase inhibitor (NEB), 0.002 U/μl yeast pyrophosphatase (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... and murine RNase inhibitor (NEB). The cell lysate was cleared for membranes by centrifugation at 12.000g for 15 min ...
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bioRxiv - Physiology 2023Quote: ... and RNase inhibitors (M0314, NEB).
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bioRxiv - Biochemistry 2023Quote: ... and 20U RNase inhibitor (NEB) for 1hr at 30°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 50U Murine RNAse inhibitor (NEB) and incubating for 4 hours at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... RNAse inhibitor (NEB cat# MO314L) was added to the homogenization buffer (0.32 M sucrose ...
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bioRxiv - Genomics 2024Quote: ... 1μL RNase inhibitor (NEB, M0314L), 0.8μL Maxima H RT Enzyme (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... and 20U RNase inhibitor (NEB). The translation reactions were incubated in 384 well plates at 30°C for 1hr ...
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bioRxiv - Genomics 2022Quote: ... and the presence or absence of one of two RNAse inhibitors (1 – Millipore Sigma, Protector RNAse Inhibitor, Cat. No. 3335399001; 2 – NEB, RNAse Inhibitor, M0314S). The RNA was then extracted from the tissue using the miRNeasy kit (Qiagen Cat ...
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bioRxiv - Plant Biology 2019Quote: ... Recombinant proteins were purified using gravity flow columns with amylose resin (New England Biolabs). MBP cleavage was performed by incubation in cleavage buffer (50 mM Trizma-HCl ...
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bioRxiv - Genomics 2021Quote: ... the same amplicons were treated in vitro with the recombinant CpG methyltransferase M.SssI (NEB). Briefly ...
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bioRxiv - Immunology 2020Quote: ... All in vitro activity assays with recombinant GST-IdeZ or IdeZ (NEB Catalog #P0770S) (1ug per reaction ...
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bioRxiv - Neuroscience 2022Quote: Recombinant proteins were labeled with SNAP-Surface 549 dye (New England BioLabs, Cat# S9112S) for visualization ...
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bioRxiv - Plant Biology 2021Quote: Recombinant proteins from Escherichia coli lysates were immobilized on amylose resins (New England Biolabs), incubated for 1 h at 4 °C with purified GST-SH3P2 ...
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bioRxiv - Developmental Biology 2020Quote: ... the Lox-Stop-Lox site was removed by exposure to recombinant Cre recombinase (NEB), using the NEB standard protocol ...
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bioRxiv - Plant Biology 2022Quote: ... For recombinant protein expression we used GW versions of pMAL-C2 (New England Biolabs) and pDEST-17 (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant proteins were affinity purified through GST tag binding to amylose resin (BioLabs) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Recombinant plasmids were recovered by transformation of Escherichia coli NEB5α F’IQ (New England Biolabs) and selected on LB agar supplemented with 50 μg/ml Kanamycin ...
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bioRxiv - Microbiology 2023Quote: ... All S recombinants were constructed via gene fragment Assembly (New England Biolabs, catalog #: E2621S).
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bioRxiv - Neuroscience 2021Quote: The rat HA-tagged PRG-1 ORF construct was generated by Gibson Assembly Cloning from NEB (Ipswich, MA, #E5510S) of the Plppr4 ORF clone (NM_001001508.2 ...
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bioRxiv - Microbiology 2020Quote: ... The Flag-tagged spike gene was ligated to the linearized vector using the T4 DNA Ligase enzyme from NEB. The resulting plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... new SNAP-tagged histones were pulse-labeled for 30 min with 5 μM final SNAP-biotin (New England Biolabs) diluted 1:200 in 10% Duolink blocking buffer (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged ORF3c mutant Q5Y was prepared by site-directed mutagenesis using a commercial kit (New England Biolabs, E0554S).
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bioRxiv - Cancer Biology 2023Quote: ... Gαi2R179C and GαoAR243H mutants were constructed using pcDNA3.1 wild-type human ee-tagged-Gαi2- and ee-tagged-GαoA plasmids as template (cDNA Resource Center) and Q5 Site-directed mutagenesis kit (New England BioLabs). The lentiviral vector for tetracycline-inducible expression of the Gαi2R179C and GαoAR243H mutants was constructed by first cloning Gαi2R179C and GαoAR243H from pcDNA3.1 to pENTR vector (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of immunopurified GST-tagged WRN fragment was phosphorylated in vitro by Casein Kinase II (New England Biolabs) in the presence of 1X NEBuffer (50mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... All truncated or tagged versions of clones were generated using Q5 high-fidelity DNA polymerase (New England Biolabs #M0515) and NEB-Gibson Assembly (New England Biolabs #E2611S) ...