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Citations for New England Biolabs :
251 - 300 of 3153 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... ERK (9102, NEB, 1:10000); phospo-ERK (9101 ...
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bioRxiv - Neuroscience 2023Quote: ... AKT (9272, NEB, 1:5000); Phospo-AKT (9271 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM dNTPs (NEB - N0446S), 1.2 units SUPERaseIN Rnase Inhibitor (Thermo Fisher – AM2696) ...
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bioRxiv - Biochemistry 2023Quote: ... 1 U T4 ligase (NEB) and the fragment 1 & 2 for each mutant ...
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bioRxiv - Genomics 2023Quote: ... 1 µl EcoRI enzyme (NEB), and 38 µl nuclease-free water ...
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bioRxiv - Genomics 2023Quote: ... 1 µl PsiI enzyme (NEB), and 12 µl nuclease-free water were mixed and incubated at 37°C for 1 hour followed by 65°C for 20 minutes to heat inactivate the restriction enzymes ...
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bioRxiv - Genomics 2023Quote: ... 1 µl PsiI enzyme (NEB), 1 µl EcoRI enzyme (NEB) ...
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bioRxiv - Genomics 2023Quote: ... 1 µl EcoRI enzyme (NEB), 1 µl PsiI enzyme (NEB) ...
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bioRxiv - Immunology 2023Quote: ... 1 µL Phusion polymerase (NEB), and remainder water (to 50 µL) ...
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bioRxiv - Neuroscience 2023Quote: SOX10 (89356, NEB, 1:400), sox10 zebrafish (AB229331 ...
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bioRxiv - Neuroscience 2023Quote: ... p21 (2946, NEB, 1:3000), Isl1/2 (67.4E12 ...
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bioRxiv - Cell Biology 2023Quote: ... Cy3-(1:400, Jackson Biolabs), Alexa 546-(1:400 ...
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bioRxiv - Biophysics 2023Quote: ... A 1 kbp ladder (NEB) was used for the relative comparison of the bands.
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bioRxiv - Immunology 2023Quote: ... 1 mM ATP (NEB, # P0756S), 1 µl Esp3l (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 uL of DpnI (NEB) was added directly to the PCR reactions for 1 hr at 37°C ...
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bioRxiv - Immunology 2024Quote: ... 1% penicillin/streptomycin (Nordic Biolabs), referred to complete medium (CM) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
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bioRxiv - Bioengineering 2024Quote: ... 1 ul of RNaseH (NEB), 10 ul rCutSmart Buffer (NEB) ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μL of DpnI (NEB) was added to the PCR mix and incubated for 2 h at 37 ℃ ...
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bioRxiv - Molecular Biology 2023Quote: ... with XRN-1 (NEB #M0338S) alone ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM NTPs (NEB, N0416S) and DNA template ...
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bioRxiv - Immunology 2024Quote: ... dNTPs (NEB, #N0447L, 1 µl), water (1 µl) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 kb ladder (NEB) were mixed with 1× alkaline buffer ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 µL DpnI (NEB) directly to the PCR reaction mixture after PCR and continued incubation for 1h at 37°C ...
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bioRxiv - Genomics 2023Quote: ... 1× BSA (New England Biolabs), 1 mM dNTPs (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 x concentrated Phusion HF buffer and 1 U Phusion DNA Polymerase (New England Biolabs). The PCR reaction was performed as follows ...
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bioRxiv - Biochemistry 2021Quote: ... 1 ng/μl of template DNA and 1 U of Phusion polymerase (New England Biolabs). The mutant PCR fragments were then cloned into pSpurMS2 (a gift from Dr ...
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bioRxiv - Genetics 2023Quote: ... PCR products were visualized on 1% agarose gels with a 1 kb plus ladder (NEB).
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bioRxiv - Biochemistry 2023Quote: ... with 1 μg RNA and 1 unit RNase Inhibitor (New England Biolabs Inc., Massachusetts, USA), 5 μg methyltransferase or no enzyme ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM EDTA] plus 1 µL 20 µg/µL lambda phage DNA (NEB, cat # N3011S) and was separated on a 6% polyacrylamide / 0.5x TBE / 1.5 mm gel for 2 hr at 110 V in a Novex electrophoresis system ...
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bioRxiv - Biochemistry 2023Quote: ... Desalted peptides were dissolved in 1 ml 1 × CutSmart buffer (pH 8, New England BioLabs) and incubated with 50 units of alkaline phosphatase (calf intestinal ...
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bioRxiv - Microbiology 2024Quote: ... the ExoI reaction mixture containing 1 x ExoI buffer and 1 U/μL ExoI (NEB) enzyme was prepared ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× NEBuffer 3.1 (New England Biolabs) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× NEBuffer 3.1 (New England Biolabs) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg μl-1 bovine serum albumin (New England Biolabs® Inc., Ipswich, MA, USA), 0.2 μM of each primer and 2 μl of template DNA ...
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bioRxiv - Immunology 2021Quote: Point or deletion mutations were prepared from human or mouse CRT expression clones in pCMV3-C-Myc (SinoBiological) using the Q5® Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s protocol and primers designed using the NEBaseChanger™ web tool ...
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bioRxiv - Systems Biology 2020Quote: ... rRNA depletion and library preparation for sequencing was completed with the NEBNext protocol (‘Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Neuroscience 2021Quote: The moPrP-eGFP_39/40 fusion construct carrying eGFP within the flexible N-terminal tail between aa 39 and 40 of mouse PrP was created using NEB® Golden Gate Assembly (New England Biolabs). In brief ...
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bioRxiv - Cancer Biology 2020Quote: ... we performed eight 100 μl PCR reactions per sample (4 μg DNA per reaction, 32 μg per mouse) using Q5 High-Fidelity 2x Master Mix (New England Biolabs, M0494X). The PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2022Quote: ... DNA fragments ranging from 3 kb to 5 kb with 40-100 bp terminal homologies were amplified from mouse BAC RP23-51O13 with Q5 polymerase (NEB, M0491L). Approximately equal amount (100 ng ...
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bioRxiv - Immunology 2020Quote: CAR-encoding mRNA was transcribed from linearized plasmids encoding anti-human CD19 CAR61 and anti-mouse CD19 CAR71 using T7 RNA polymerase (HiScribe™ T7 High Yield RNA Synthesis Kit, New England Biolabs). The mRNAs were transcribed to contain the 5′ UTR derived from the human a-globin 5′ leader RNA ...
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bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared according to the protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) ...
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bioRxiv - Systems Biology 2023Quote: ... Signals following a 1h incubation at room temperature with the appropriate HRP-conjugated secondary antibodies (anti-mouse NEB 7076S or anti-rabbit NEB 7074S) were acquired using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Ribosomal RNA from 1000 ng total extracted RNA was depleted using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat; New England Biolabs Inc.). The remaining RNA was used to produce the sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc. ...
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bioRxiv - Genomics 2023Quote: Mouse tail (1cm) was used to extract the genomic DNA (gDNA) using NEB Monarch® Genomic DNA Purification Kit (NEB T3010L). DNA concentration was measured on Qubit dsDNA BR assay kit (cat ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed three times with a PBS buffer 1X and then incubated with mouse anti-MBP (NEB, Cat# E8032L) or rabbit anti-V5 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... 250 μL of 2 mg mL-1 Y289L GalT,46 50 μL of 500 kU mL-1 PNGase F (New England Biolabs, 5 U μg-1 of protein), and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs ...
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bioRxiv - Biophysics 2020Quote: ... 1 μL T4 polynucleotide kinase and 1 μL DpnI (New England Biolabs, Cats. #M0201S and R0176S) and incubated at 37°C for 1 hour ...
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bioRxiv - Genomics 2022Quote: ... 1 μg of PCR product containing barcoded oligos were inserted into 1 μg SfiI (NEB, R0123) digested empty vector A or P by gibson assembly NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1.4 units μL−1 T7 RNA polymerase (RNAP, NEB 2 units μL−1 RNase inhibitor (NEB), 50 nM Cas9 (NEB) ...