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Citations for New England Biolabs :
251 - 300 of 10000+ citations for Mouse Oligodendrocyte transcription factor 1 OLIG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... and the corresponding RNAs were synthesized via in vitro transcription using the HiScribeTM T7 Quick High Yield RNA Synthesis Kit (New England Biolabs Inc.) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... 200-500 ng of the purified PCR product was used as DNA template in 25 μl of coupled in vitro transcription and translation reaction using PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800L). The reaction was incubated at 37°C for 30 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... We then performed in vitro transcription from these amplicons using the HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs), and purified the resulting RNA using a GeneJET RNA Purification Kit (Thermo Scientific) ...
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bioRxiv - Genomics 2021Quote: ... were used as template of the in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit; #E2050S, New England Biolabs), performed at 37°C for 16h ...
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were used as templates for in vitro transcription using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, New England Biolabs). Cas9 mRNA and gRNAs were then purified using RNAClean XP(Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... 200 ng/μL was used for subsequent reverse transcription assay with Luna® Universal One Step RT-qPCR kit (E3005, New England Biolabs) according manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... and 1 μg of purified PCR product was used as template for in vitro transcription using the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, E2040S) as described by the manufacturer.
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Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia functionbioRxiv - Molecular Biology 2020Quote: ... The guide region was then amplified by PCR and paired guide RNAs synthesised by in vitro transcription (T7 Quick High Yield RNA Synthesis kit, NEB, #E2050S). Single stranded DNA oligonucleotides (WT oligo ...
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bioRxiv - Developmental Biology 2021Quote: ... Resultant DNA was gel extracted then 400 ng template used for in vitro transcription with the HiScribe RNA synthesis T7 kit (NEB, E2040) for 2 hours at 37 °C ...
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bioRxiv - Microbiology 2023Quote: MNV plasmids were linearised with NotI and phenol/chloroform extracted before being used for in vitro transcription using the HiScribe™ T7 ARCA mRNA Kit (NEB), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 8 µl PCR amplified product was used for the in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, E2040S), incubating at 37 °C for 18 hours in the thermocycler ...
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bioRxiv - Bioengineering 2023Quote: mRNA was synthesized through in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (NEB, China, Cat: E2080S). The templates for in vitro transcription were linearized plasmids ...
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bioRxiv - Microbiology 2023Quote: ... For in vitro transcription plasmids were first linearised by restriction digest and purified using Monarch PCR and DNA Clean-up Kit (NEB, USA). 3’UTRs were in vitro transcribed from 500ng of plasmid DNA using MEGAscript T7 Transcription Kit (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... In vitro transcription of pegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit (New England Biolabs, no. E2070S), purified using the Monarch RNA Cleanup Kit (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... A 2 µL aliquot of the annealed oligonucleotides was used as template for in vitro RNA transcription by HiScribe T7 quick high yield RNA Synthesis kit (New England Biolabs, MA). The transcribed RNA was purified using RNA clean and concentrator kit (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 µl of the PCR-amplified product was employed for in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, E2040S), with incubation at 37 °C for 18 hours in a thermocycler ...
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bioRxiv - Immunology 2024Quote: ... The sequences containing T7 promoter and sgRNA were PCR-amplified and used as templates to produce sgRNAs through in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). All sgRNAs were purified by lithium chloride precipitation and stored at −80°C.
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of PCR product was used in an in vitro transcription reaction using the HiScribe T7 High Yield RNA Synthesis kit (NEB, E2040S) in the presence of 20units/mL SUPERaseIn ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting amplicon was used as starting material for in vitro transcription using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). Finally ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... UK) were then synthesized through in vitro transcription of 1 mg template with T7 polymerases (M0251, New England Biolabs). The sections were fixated in 4% PFA ...
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bioRxiv - Neuroscience 2022Quote: ... or the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB) (replicates 4-5 and no salt treatment ...
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bioRxiv - Molecular Biology 2022Quote: ... we used NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E7405), NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7765 ...
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bioRxiv - Genetics 2023Quote: ... using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB E7405) or the NEBNext Poly(A ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with mouse anti-MBP (NEB, 1:500), followed by washing and subsequent incubation with goat anti-mouse IgG ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were stained with mouse anti-MBP (NEB, 1:500), followed by washing and subsequent incubation with goat anti-mouse IgG ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse α-myc (New England Biolabs, clone 9B11, 1:2,000), rabbit α-pol-I largest subunit 15 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... Appropriate fractions were pooled and digested with factor Xa protease (New England Biolabs) in the presence of 2 mM CaCl2 at 4 °C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... The sgRNAs were synthesised by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). Cas9 mRNA was prepared by in vitro transcription with the mMESSAGE mMACHINE T3 Transcription Kit (Life Technologies ...
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bioRxiv - Physiology 2019Quote: PCR templates generated as described above (see Table S3) were used for in vitro transcription reactions using the HiScribe T7 RNA Synthesis Kit (New England Biolabs, Whitby, ON) following the recommended conditions when using modified nucleotides ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acid (5 μL) was used as template for reverse transcription using LunaScript® RT SuperMix Kit (New England Biolabs, Hitchin, UK) in 20 μL reaction volume ...
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bioRxiv - Developmental Biology 2021Quote: ... sgRNA synthesis was performed by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). Cas9 mRNA was prepared by in vitro transcription with the mMESSAGE mMACHINE T3 Transcription Kit (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... sgRNAs were generated by in vitro transcription using the Hiscribe T7 high yield RNA synthesis kit (New England Biolabs, Ipswich, MA, USA). Purified sgRNA (0.5 μg ...
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bioRxiv - Microbiology 2023Quote: ... AvcI RNA was synthesized by in vitro transcription using the T7-AvcI reverse complement DNA template and the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB™). Bio-11-UTP was included during the transcription reaction for Northern blot detection purposes ...
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bioRxiv - Biochemistry 2023Quote: ... Wild-type or mutant plasmid was linearized using restriction digestion and then subsequently used for in vitro transcription (HiScribe® T7 High Yield Synthesis Kit, NEB E2040) to synthesize wild-type and mutant N gene RNA ...
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bioRxiv - Bioengineering 2022Quote: ... These linearized templates were used to direct in vitro transcription (IVT) of mRNAs using the HiScribe™T7 mRNA Kit with CleanCap® Reagent AG (NEB). Cy3-UTP and Cy5-UTP were used replacing 25% of standard UTP in some experiments to fluorescently label the mRNA transcripts ...
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bioRxiv - Systems Biology 2023Quote: ... Base editor mRNAs were generated by in vitro transcription using the HiScribe T7 High-Yield RNA synthesis kit (NEB Cat No. E2040S) via the method described previously86 ...
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bioRxiv - Microbiology 2023Quote: ... cRNA synthesis was accomplished through in vitro transcription using the HiScribe T7 ARCA mRNA Kit with tailing (New England BioLabs, Cat. # E2060S).
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bioRxiv - Immunology 2023Quote: ... The T7 sgRNA PCR product (500 ng) was used as a template for in vitro transcription with the T7 High Yield RNA synthesis kit (NEB, cat # E2040S). The reaction was incubated at 37°C for 4 hours ...
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bioRxiv - Plant Biology 2023Quote: ... from Col-0 cDNA and prepared for in vitro transcription with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). After purification with Monarch RNA Cleanup Kit (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: Base editor mRNAs were generated by in vitro transcription using the HiScribe T7 High-Yield RNA synthesis kit (NEB Cat No. E2040S) via the method described in48 ...
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bioRxiv - Cell Biology 2020Quote: ... Transcription reactions were treated with RNase-free DNaseI (NEB) to digest the DNA template at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription reactions were carried out with SP6 polymerase (NEB) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro transcription reactions were treated with DNAse (NEB) for 1 hour at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... and +1 sites of the promoters controlling transcription of both RNAs were used to amplify the standard pUC19 plasmid (NEB). After PCR amplification samples were digested with DpnI (NEB ...
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bioRxiv - Physiology 2023Quote: ... was obtained by reverse transcription (RT) using 1 μg of RNA and Moloney murine leukaemia virus (M-MuLV) reverse transcriptase (M0253, NEB), using the first strand cDNA synthesis standard protocol with random primers (S1330 ...
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bioRxiv - Genetics 2020Quote: ... following rRNA depletion using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB).
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bioRxiv - Physiology 2019Quote: ... The synthesis of cDNA for reverse transcription was carried out with a Protoscript II first strand cDNA synthesis kit (New England Biolabs, Ipswich, MA, USA) using 120 ng total RNA and 60 μM random hexamers ...
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bioRxiv - Immunology 2023Quote: ... 1μg of NotI-linearized plasmid was subjected to in vitro transcription using the HiScribe T7 ARCA mRNA Kit (with tailing) (New England Biolabs, Ipswich, MA, USA). For use in ELISpot ...
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bioRxiv - Biochemistry 2023Quote: ... 544 adenine bases) was prepared by in vitro transcription using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs Inc., Massachusetts, USA) with 1 unit Rnase Inhibitor (New England Biolabs Inc. ...
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bioRxiv - Bioengineering 2023Quote: ... RNA synthesis was conducted via in vitro transcription using a HiScribe™ T7 high yield RNA synthesis kit (New England BioLabs, NEB, Ipswich, MA). After DNase I (RNase-free ...