Labshake search
Citations for New England Biolabs :
251 - 300 of 600 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate, NEB) or anti-mouse IgG (H+L ...
-
bioRxiv - Biochemistry 2022Quote: ... The hybrid was reacted with 1 μl RNase H (New England BioLabs) at 37 °C for 30 min and then heated to 90 °C for 10 min to terminate the reaction ...
-
bioRxiv - Cell Biology 2021Quote: ... clarified lysates were treated for 1 h with λPP (New England BioLabs) as described above ...
-
bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were treated for 1 h with λPP (New England BioLabs) as described above ...
-
bioRxiv - Molecular Biology 2020Quote: ... and once with 2 bead volumes of 1X RNase H Buffer (NEB; 50mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2021Quote: ... known standards (H. wingei and/or Yeast Ladder from New England BioLabs) were included in each CHEF gel run.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: rTTP1 and LTM-TPP1 were treated with Endoglycosidase H (New England Biolabs) to remove N-glycan modifications ...
-
bioRxiv - Microbiology 2020Quote: ... The remaining glycopeptides were first digested with Endo H (New England Biolabs) to deplete oligomannose- and hybrid-type glycans and leave a single GlcNAc residue at the corresponding site ...
-
bioRxiv - Microbiology 2022Quote: ... for 7-12 h at 37°C in CutSmart buffer (NEB, B7204S). Digested products were then visualised on a 4% NuSieve (Lonza ...
-
bioRxiv - Microbiology 2023Quote: ... Endoglycosidase H digestion was performed according to manufacturer’s instructions (New England BioLabs). Complexes were dissociated at 95°C in sample buffer containing 150 mM DTT prior to loading on a gradient SDS-PAGE ...
-
bioRxiv - Biophysics 2024Quote: ... The RNA:DNA hybrid was then cleaved by RNase H (NEB catalogue # M0297L) to produce the final 22 nucleotide product ...
-
bioRxiv - Microbiology 2023Quote: ... and RNase H in NEBNext® Second Strand Synthesis Reaction Buffer (NEB) at 16 ºC for three hours ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1 ug of DNA was incubated with 6U RNAse H (NEB, M0297) at 37 °C for 2 h ...
-
bioRxiv - Cell Biology 2023Quote: ... After 24 h cell lysates were treated with PNGase-F (NEB, P0704L) or water by following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNAs were cleaved using RNAse H (New England Biolabs, Ipswich, MA, USA) with LA121 as a specific primer for HIS3-tCYC1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cDNA was cleared of RNA by treating with RNase H (NEB). Half the cDNA was subjected to 2nd strand synthesis using Phusion high-fidelity DNA polymerase (NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... endoglycosidase H (500 units, 1 μl, New England Biolabs, catalog no. P0702) was added to each of the SLNYLLYVSN peptide samples ...
-
bioRxiv - Genetics 2023Quote: ... the hybrid was reacted with 1 μl RNase H (New England BioLabs) at 37 °C for 30 min and then heated to 90 °C for 10 min to terminate the reaction ...
-
bioRxiv - Cell Biology 2022Quote: ... Endo H assay was performed following manufacturer’s instructions (New England Biolabs, P0702S). Following addition of Glycoprotein Denaturing Buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... before 9 μl of 5 U/μl thermostable RNase H (M0523, NEB) were added and samples were incubated 1 h at 50 °C with 800 rpm shaking (15 s on/15 s off) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 9 μl of 5 U/μl of thermostable RNase H (M0523, NEB) were added and samples were incubated 1 h at 50 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... 25% of the sample was used for Endo H (New England Biolabs) treatment following manufacturer protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... A preheated mixture of 10 µl of RNase H (New England Biolabs) and 2 µl of RNase H Buffer (New England Biolabs ...
-
bioRxiv - Biophysics 2024Quote: Rehydrated embryos were blocked for 2 h in 2% BSA (B9000, NEB) in PBS with 0.3% Triton X-100 (T9284 ...
-
bioRxiv - Cell Biology 2024Quote: ... the cDNA samples were treated with RNase H (New England Biolabs, M0297L) and incubated at 37°C for 30 minutes and 65°C for 20 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... and 2U of Uracil-D-glycosylase (New England Biolabs, USA). Conditions of PCR were as follows ...
-
bioRxiv - Molecular Biology 2022Quote: ... oligo-d(T)25-Magnetic Beads (S14195, New England Biolabs) were used ...
-
bioRxiv - Developmental Biology 2021Quote: ... 10 μg of nucleic acid was digested with 5 μL RNase H (NEB) at 37°C for 16 hours and 10 μg was mock digested without RNase H ...
-
bioRxiv - Neuroscience 2021Quote: ... Remaining RNA genomes were selectively digested using RNase H (New England Biolabs, M0297S) and reactions were cleaned with Agencourt AMPure XP beads (1:1 volume ...
-
bioRxiv - Molecular Biology 2021Quote: ... The eluted DNA was incubated with or without RNase H (New England Biolabs) at 37 °C overnight ...
-
bioRxiv - Molecular Biology 2021Quote: ... DNA was phosphorylated for 1 h at 37oC with T4 Polynucleotide kinase (NEB or made in-house by the Molecular Biology Service ...
-
bioRxiv - Biochemistry 2021Quote: ... The parental plasmid molecules were inactivated by DpnI treatment (1 h, NEB, USA) and the crude reaction mixture was transformed to electrocompetent E ...
-
bioRxiv - Molecular Biology 2021Quote: ... followed by the addition of 1 µl RNase H (5U final, NEB M0297L) and incubation for 20 min at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... The rRNAs hybridized to the oligonucleotides were digested with RNAse H (NEB, M0297S) as previously described in (Minia et al. ...
-
bioRxiv - Biochemistry 2020Quote: ... The template plasmid molecules were inactivated by DpnI treatment (1 h, NEB, USA) and directly transformed to electrocompetent E ...
-
bioRxiv - Microbiology 2021Quote: ... the cDNA was treated with 1 ul of RNase H (New England Biolabs) and incubated at 37 C of 20 minutes.
-
bioRxiv - Molecular Biology 2022Quote: ... each RT reaction was treated with 5000units of RNAse H (New England Biolabs) for 30 minutes at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... solubilized proteins were treated with Endo H and PNGase F (New England Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... The PCR reaction was DpnI digested (New England Biolabs, 37°C, 1 h) and column purified (Macherey-Nagel ...
-
bioRxiv - Microbiology 2021Quote: ... samples were transferred to ice and RNase H (New England Biolabs, Ipswich, MA) was added ...
-
bioRxiv - Microbiology 2021Quote: ... The RT reaction was treated with 1 μl RNase H (New England Biolabs) and incubated at 37°C for 20 min to remove RNA ...
-
bioRxiv - Microbiology 2021Quote: ... Bound RNA was degraded with 5 units of RNase H (New England Biolabs) and the cDNA purified by ethanol precipitation overnight at −20 °C (3x volumes of ethanol ...
-
bioRxiv - Immunology 2020Quote: ... Beads were washed and complexes were treated with Endoglycosidase H (New England Biolabs) according to manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... Bound chromatin and input DNA were treated with RNase H (New England Biolabs) and Proteinase K (Sigma-Aldrich ...
-
bioRxiv - Genomics 2021Quote: ... tissues were treated with 1.5uL RNase H (5,000 units/ml; New England Biolabs) in 50uL final volume in 1X RNAse H buffer at 37°C for 2 hours and washed three times with Stellaris wash buffer at room temperature for 10 minutes before mounting and imaging.
-
bioRxiv - Microbiology 2020Quote: ... or anti-mouse IgG (H+L) (DyLight™ 800 4X PEG Conjugate, NEB) were used as the secondary antibodies.
-
bioRxiv - Genetics 2022Quote: ... was digested for 1 h with 50 units of DpnI (New England Biolabs) to eliminate unreplicated plasmids ...
-
bioRxiv - Synthetic Biology 2022Quote: ... into backbones previously digested with corresponding restriction enzymes (NEB, 1 h 37° C) to yield intermediate plasmids containing individual transcriptional units ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 mM MgCl2 and 2 μL (10 U) RNase H (NEB, Cat# M0297S) in order to digest poly(A ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 2S rRNA hybridizing with oligo DNA was digested with RNase H (NEB). RNA mixture depleted of 2S rRNA was loaded onto 8M urea-polyacrylamide gel (12% ...