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Citations for New England Biolabs :
251 - 300 of 3535 citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The hybridization reaction contained 40 pmol of primary probes and 50 pmol of secondary probes in 1X NEBuffer buffer (NEB; B7003S), and was carried out in a PCR machine with the cycles of 85°C for 3 min ...
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bioRxiv - Bioengineering 2024Quote: ... The WarmStart Master Mix is designated because the enzyme is inactive at lower temperatures but becomes active once the reaction solution reaches a temperature of 40°C (NEB, USA). Using the enzyme and indicator of this recent invention ...
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bioRxiv - Biophysics 2021Quote: ... Homology arms of ~800 bp were amplified from genomic DNA using PCR primers with 40 bp overhangs compatible with pUC19 backbone digested with Xba1 and Ecor1 (New England Biolabs, Ipswich, MA) (sequences in the table below) ...
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bioRxiv - Microbiology 2022Quote: ... The primers were designed with 25 bp of overlapping sequence for isothermal assembly (40) into pNPTS139 plasmid digested using EcoRV-HF (New England Biolabs, Ispswich, MA) using the NEBuilder HiFi Assembly Master mix (NEB) ...
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bioRxiv - Genomics 2023Quote: The 40 libraries were constructed with NEBNext® Ultra II DNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA) according to the instruction ...
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bioRxiv - Systems Biology 2023Quote: ... A 100 μl setup using 40 pmol of oDL00549 and 200 pmol each of oDL00122 and oDL00551 using the Q5 Master Mix (New England Biolabs, Ipswitch, MA) was run with an initial denaturation at 98 °C for 30 sec ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids were constructed using isothermal DNA assembly with 40-60 bp overhangs (NEBuilder HiFi DNA Assembly Master Mix, New England BioLabs, Ipswich, MA) and transformed into high-efficiency NEB 10-beta competent E ...
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was conducted in MES buffer at 100-120 V for 40 min with protein standards (NEB, P7119S, or Bio-Rad, #1610373). Proteins were transferred to nitrocellulose membrane and membranes blocked in 5% milk powder/PBS overnight at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... All plasmids were linearized in reactions containing 40 ng/ml DNA and 0.5 U/ml restriction enzyme (HindIII-HF, NEB, Cat. No. R3104L) in 1x CutSmart buffer (NEB ...
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bioRxiv - Cell Biology 2024Quote: In vitro kinase reaction was performed in 40 μl solution consisting of 1X NEBuffer™ for Protein Kinases (PK) (New England Biolabs, B6022SVIAL), 2 mM ATP-gamma-S Kinase substrate (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... was purchased from New England Biolabs (M0314S, NEB, CA, USA). Methylene blue ...
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bioRxiv - Biochemistry 2024Quote: ... R3104L) for SMG6-3xFLAG in reactions containing 40 ng/ μl DNA (4 μg in total) in 1x CutSmart buffer (NEB, Cat No. B7204S) for 2h or overnight at 37°C ...
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bioRxiv - Genomics 2020Quote: ... A quick Ligation Kit (New England Biolabs Inc., MA, CA#M200l) was used for ligating the insert and vector ...
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bioRxiv - Genomics 2023Quote: ... and 200 ng/µl Cas 9 protein (New England BioLabs, M0386T). Injected individuals were screened (see below genotyping procedure ...
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bioRxiv - Microbiology 2021Quote: ... MNase activity was quenched by addition of EGTA and samples were subsequently pre-cleared by addition of 40 µl Protein A Magnetic Beads (New England Biolabs, Ipswich, MA, United States) and rotating at 4°C for 60 min ...
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bioRxiv - Cancer Biology 2022Quote: ... DNase I treatment was performed to denaturalize the genomic DNA in the cells using 20U DNase I for 40s per coverslip (NEB; DNase I (RNase-free), M0303S) ...
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bioRxiv - Genomics 2020Quote: ... and SalI (5’-GTCGAC-3’, New England Biolabs Inc., MA, CA#R3138S) restriction enzymes ...
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bioRxiv - Genomics 2021Quote: ... The NEB Next® Ultra™ RNA Library Prep (NEB, CA, USA) kit based on the polyA method was used for RNA library preparation ...
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bioRxiv - Developmental Biology 2023Quote: ... Injection mixtures containing 500 ng/µl of Cas 9 protein (NEB; Cat. no.: M0641) and 300 ng/µl of guide RNA were prepared and injected into eggs within 4 hours of egg laying ...
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bioRxiv - Immunology 2023Quote: ... followed by the NEBNext Ultra II Directional RNA Library Prep Kit (CAS: E7760S, NEB). Completed libraries were then sequenced to an average depth of approximately 20M reads per sample on a partial lane of the NovaSeq6000 S4 XP flow cell using 2×150 paired-end reads with 10-base dual indexes (CAS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... CA using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, #E7775) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... (Sacramento, CA, USA) for library construction (NEBNext Ultra RNA Library Prep Kit, New England Biolabs) from 1 µg total RNA and Illumina sequencing ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of Exonuclease 1 (NEB, M0293S) was added to each PCR product and incubated at 37C for 15 min to digest any single-stranded product ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× NEB buffer 1 (NEB, B7001S) for 15 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1% cholorophorm was added together with 10 μg mL-1 DNase 1 (NEB) and 1 μg mL-1 RNase A ...
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bioRxiv - Molecular Biology 2024Quote: ... The four PCR products were then assembled in a 1:1:1:1 ratio using HiFi assembly (NEB, E5520S) according to the manufacturer’s instructions to create the pLenti-STARR vector (pAS5018) ...
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bioRxiv - Microbiology 2022Quote: The recombinant constructs (pETDuet-1-TaPHB-1 and pETDuet-1-TaPHB-1-RUVBL1 were separately transformed into E. coli Lemo21(DE3) (NEB) chemical competent cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Unincorporated ddRTP molecules were inactivated by adding 1 µL of Buffer 2 (80 mM MgCl2) and 1 µL of 1:1 rSAP (NEB):50% glycerol ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μL T4 RNA Ligase 1 (NEB, M0437M), 1 μL SuperAseIn (Invitrogen AM2694) ...
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bioRxiv - Cancer Biology 2021Quote: ... pLVX mCherry N1 (Clonetech Laboratories Inc., CA) was digested with XhoI and BamHI (New England BioLabs Inc., MA) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, US-CA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Immunology 2023Quote: ... poly-A enriched libraries were created using the NEBNext Poly(A) mRNA Magnetic Isolation Module (CAS: E7490S, NEB) followed by the NEBNext Ultra II Directional RNA Library Prep Kit (CAS ...
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bioRxiv - Genetics 2024Quote: ... (Sacramento, CA, USA) for library construction (NEBNext Ultra RNA Library Prep Kit, New England Biolabs, Ipswich, MA, USA) from 1 µg total RNA and Illumina sequencing using the NovaSeq 6000 Sequencing System ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM MgCl2) supplemented with 1 mM ATP and 1 U/μL RNase Inhibitor (NEB). Reaction was incubated for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were diluted 1:1 and 1:5 respectively in puc19 vector (New England Biolabs). HaloTag expression was confirmed using HaloTag ligand dyes HTL-AF488 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 unit µL-1 EcoRI-HF (New England Biolabs)) ...
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bioRxiv - Biophysics 2020Quote: ... 1 U μL−1 murine RNase inhibitor (NEB, M0314L), 600 ng mRNA and 50 μM PF846 in 1% DMSO ...
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bioRxiv - Genomics 2021Quote: ... 1 unit/μl RNA ligase 1 (New England Biolabs), 1× RNA ligase buffer ...
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bioRxiv - Bioengineering 2022Quote: ... 1 U.μL-1 of murine RNase Inhibitor (NEB M0314), 500 μM of rNTPs (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... 1 U.μL-1 of murine RNase Inhibitor (NEB M0314), 500 μM of rNTPs (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 μl T4 DNA Polymerase (1 U, NEB). Gap-filling reactions purified using the Zymo Research kit and eluted with 6 uL water ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 1 U μl−1 RNase inhibitor (NEB), or bulk sorted into buffer RLT Plus (Qiagen ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µl of ExoI and 1 µl rSAP (NEB) were added to the recovery products and incubated for 15 min at 37°C followed by heat inactivation of 20 min at 80°C ...
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bioRxiv - Genomics 2024Quote: ... 1 unit/μl RNA ligase 1 (New England Biolabs), 1× RNA ligase buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... and KpnI) into pSB/IR-CA-HybIntr-SV40intr-BleoR-T2A-mCherry plasmid restricted with restriction endonucleases BsaI (NEB, USA) and AsiG.
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bioRxiv - Cancer Biology 2021Quote: ... Whole-genome sequencing and library preparations for tumors and matched germline controls were performed according to the manufacturer’s instructions (Illumina, San Diego, CA, USA or NEBNext, NEB). The quality of the libraries was assessed using a Bioanalyzer (Agilent ...
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bioRxiv - Synthetic Biology 2022Quote: ... sgRNA expression plasmids were constructed via digesting the sgRNA backbone plasmids that contained a human U6 promoter and a Cas-nuclease’s corresponding sgRNA scaffold sequences using BsmB I or Bbs I endonuclease (NEB) and then ligated the oligonucleotide duplexes into this cut backbone ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using NEB Next® Ultra™ RNA Library Prep Kit (NEB, San Diego, CA, USA), employing AMPure XP Beads to purify the products of the reactions during the library prep ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral vectors for inducible expression of CA-MNK2 (T379D) and SLUG were generated by Gibson assembly (New England Biolabs) or standard cloning in the pTRIPZ plasmid from pTK-Slug (a gift from Robert Weinberg ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with DNA Spin and Concentrate kit (Zymo Research, Irvine, CA, D4013 or NEB Monarch, T1030) following manufacturer instructions or gel-purified from kit (Zymo Research) ...