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Citations for New England Biolabs :
2901 - 2950 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... mRNA was prepared from total RNA by magnetic mRNA purification (Magnetic mRNA Isolation Kit,NEB) according to the manufacturer’s’ instructions with the following deviation ...
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bioRxiv - Genetics 2021Quote: ... All RNA samples were reverse transcribed using the LunaScript™ RT supermix kit (#E3010L, NEB), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-hRab22(S19N) (#1068) was generated using Q5 Site-Directed Mutagenisis kit (NEB, Cat# E0554S) according to manufacturer’s instructions with forward primer #1556 (TGTAGGTAAAaacAGTATTGTGTGGCGG ...
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bioRxiv - Molecular Biology 2020Quote: Sequencing libraries were prepared using the NEBNext Ultra™ II DNA Library Prep Kit (NEB). Four PCR cycles were used for the input samples and 17 cycles for the IP samples ...
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bioRxiv - Developmental Biology 2020Quote: ... quantified using NEBNext® Library Quant Kit for Illumina® (E7630, New England 641 Biolabs), and denatured and diluted per Illumina’s MiSeq instructions ...
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bioRxiv - Pathology 2021Quote: ... DNA ends were repaired and phosphorylated with the NEBNext End Repair kit (New England Biolabs). Adaptors were created by annealing oligonucleotides (P5/P6 and P7/P8 ...
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bioRxiv - Immunology 2021Quote: ... and 60 ng were used for the One Taq RT PCR Kit (New England Biolabs) according to the instructions provided by the manufacturer for mRNA quantification and reverse transcription (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... 6 were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of an oligo that includes a T7 promoter ...
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bioRxiv - Synthetic Biology 2021Quote: ... applying poly-A enrichment and using the NEBNext Ultra II RNA Library Prep kit (NEB), indexed using NEBNext Multiplex Oligos (NEB) ...
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bioRxiv - Synthetic Biology 2020Quote: ... cDNA was synthesized from RNA using the Protoscript II First Strand cDNA synthesis Kit (NEB). To ensure library coverage of 500x ...
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bioRxiv - Molecular Biology 2021Quote: ... Common amplicon libraries were quantified with qPCR using NEBNext Library Quant kit (New England Biolabs) and pooled to equal concentrations ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were prepared using the NEBNext Ultra Directional Library Prep Kit for Illumina (NEB, E7420L) according to manufacturer’s instructions and quantified with the KAPA Library Quantification Kit for Illumina (Kapa Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... Ribosomes were added to 100 nM to the PURExpress ribosome-free kit (New England Biolabs) (29) ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed using Luna Universal One-Step RT-qPCR kit (NEB, Cat. # E3005E) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The DNA was in vitro transcribed by using the HiScribe T7 ARCA mRNA kit (NEB) and capped RNA was isolated by using an RNA Clean & Concentrator (Zymo Research ...
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bioRxiv - Biochemistry 2021Quote: ... RNA-seq libraries were constructed using NEBNext Ultra II directional RNA library prep kits (NEB).
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bioRxiv - Bioengineering 2021Quote: ... The digested vector backbone was purified using Monarch DNA Gel Extraction Kit (New England Biolabs) or NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) ...
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bioRxiv - Genetics 2020Quote: ... purified with a Monarch® DNA Gel Extraction kit (New England Biolabs #T1020S, Ipswich, MA), and incubated with RNAsecure™ RNase Inactivation Reagent (ThermoFisher Scientific #AM7005 ...
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bioRxiv - Genomics 2019Quote: ... Inserts were cloned into the pGL4.23c plasmid using NEBuilder HiFi DNA Assembly Cloning Kit (NEB), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... and libraries were prepared using the NEB Ultra FS II DNA Library Kit (NEB E6177) and amplified with 14 cycles of PCR ...
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bioRxiv - Immunology 2020Quote: ... cDNA was synthesized using ProtoScript First Strand cDNA Synthesis Kit (New England BioLabs, Inc – E6300L) with random primers for the MT-RNR transcripts ...
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bioRxiv - Immunology 2019Quote: ... Cysteine-to-alanine mutations were then introduced using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Promoter truncations and mutations were generated with the Q5 Site-Directed Mutagenesis Kit from NEB according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Two micrograms of RNA were used with NEBNext Poly(A) Magnetic mRNA isolation kit (NEB). Oligo(dT ...
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bioRxiv - Biochemistry 2020Quote: ... Variants of UbiVPa were obtained using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) (UbiVPa C193AC197A and UbiVPa C39AC193AC197A ...
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bioRxiv - Molecular Biology 2019Quote: ... in vitro translation was performed using the PURExpress In vitro Protein Synthesis kit (NEB 6800) and the non-stop ribosome complex was purified as previously described in ref.38 ...
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bioRxiv - Genomics 2019Quote: ... and ligated to Illumina multiplex adaptors according to NEBNext DNA library prep kit (NEB E6040S). Ligated DNA was purified using Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA libraries were constructed using the NEBNext Ultra RNA Library Prep Kit (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... libraries are amplified and purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs), libraries are eluted in 25 μl nuclease free water.
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bioRxiv - Genetics 2021Quote: ... Sequencing libraries were generated using the NEBNextUltraTM RNA Library Prep kit for Illumina (NEB, USA) following the manufacturer’s recommendation and index codes were added to attribute sequence to each sample ...
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bioRxiv - Cell Biology 2020Quote: ... using the HiScribe T7 ARCA mRNA kit with poly-A tailing (NEB, Ipswich, MA, USA). A combination of 3 separate reactions was used to achieve high concentrations (≥ 45 μg ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was also isolated using the Monarch Total RNA isolation miniprep kit (New England Biolabs). RNA was also extracted using the TRI Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs), indexed ...
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bioRxiv - Microbiology 2020Quote: ... using Luna Universal Probe One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing libraries were generated using Next UltraTM RNA Library Prep Kit for Illumina (E7760, NEB) following manufacturer’s recommendations ...
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Using optogenetics to link myosin patterns to contractile cell behaviors during convergent extensionbioRxiv - Developmental Biology 2021Quote: ... DNA fragments were linked using the NEB Builder HiFi DNA Assembly Kit (New England BioLabs). RhoGAP71E was PCR amplified from the exons corresponding to RhoGAP71E protein from pACMAN CH321-72J07 (BACPAC resources) ...
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bioRxiv - Biochemistry 2021Quote: ... we first used the HiScribeTM T7 Quick High Yield RNA Synthesis Kit (NEB, No. E2050S) to in vitro transcribe the pBESTlucT7 plasmid and generate pBESTlucT7 mRNA using the protocol provided in the manufacturer’s instruction manual [27] ...
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bioRxiv - Cancer Biology 2020Quote: ... the cDNA library is constructed using the NEBNext Ultra II RNA Library Prep Kit (NEB) as follows ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were prepared using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB, E7645S) and paired-end sequenced with read lengths of 150 bp on an Illumina HiSeq X Ten instrument.
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bioRxiv - Developmental Biology 2021Quote: ChIP-seq libraries were prepared using the NEBNext Ultra II DNA library prep kit (NEB). Samples were indexed for multiplex sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... The Am TIG1P155A mutant version was generated using site-directed mutagenesis kit (New England Biolabs). For baculovirus production ...
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bioRxiv - Biophysics 2020Quote: ... and inserted into pCDNA3.1-GPR68 using the NEBuilder HiFi DNA Assembly kit (New England Biolabs). The pCNDA3.1-jRGECO1a plasmid was cloned by assembling PCR-amplified fragments from pGP-CMV-NES-jRGECO1a (Addgene # 61563 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We used the NEBNext Ultra II directional RNA library prep kit (NEB, Ipswich, MA, USA), which allows mRNA strand orientation (sequence reads occur in the same orientation as anti-sense RNA) ...
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bioRxiv - Cancer Biology 2020Quote: ... The libraries were quantified using the NEBNext Library Quant Kit for Illumina (New England Biolabs) on a StepOne Plus Real-Time PCR (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2020Quote: ... or Taq 2x Master Mix Kit (New England Biolabs® Inc., Ipswich, MA, USA; #M0270L) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Mutagenesis of DWORF was preformed using Q5 Site-Directed Mutagenesis Kit (New England BioLabs, MA).
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bioRxiv - Biochemistry 2021Quote: ... plasmid DNA was treated using the EpiMark® Bisulfite Conversion Kit (E3318S, New England Biolabs). The protocol was modified to allow non-denaturing deamination ...
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bioRxiv - Evolutionary Biology 2020Quote: ... ChIP-Seq libraries were constructed using NEBNext Ultra DNA Library Prep kit (New England Biolabs) as described in (17) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using NEB Next® Ultra™ RNA Library Prep Kit for Illumina (New England BioLabs). Library concentration was determined using Qubit 2.0 fluorometer (Life Technologies) ...
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bioRxiv - Genomics 2021Quote: ... sRNAs were cloned for Illumina sequencing using the Next® Small RNA Prep kit (NEB) and sequenced on an Illumina HiSeq1500 platform ...