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Citations for New England Biolabs :
2901 - 2950 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... DNA libraries were generated using the NEBNext Ultra II DNA Library Preparation kit (NEB). Samples were sequenced by 40 bp paired-end sequencing using a NextSeq 500 (Illumina).
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bioRxiv - Molecular Biology 2020Quote: ... and NGS libraries were quantified by qPCR using the NEBNext Library Quant Kit (NEB). Illumina sequencing was performed with a NextSeq mid-output kit with 150-cycle single-end reads and automated adaptor trimming and demultiplexing (Illumina).
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bioRxiv - Synthetic Biology 2021Quote: ... The PCR products were later purified with the Monarch Purification Kit (NewEngland Biolabs, #T1030S) prior to deep sequencing analysis ...
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bioRxiv - Biochemistry 2020Quote: ... IVT was performed with HiScribe T7 High Yield RNA synthesis kit (New England BioLabs) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified (Monarch® PCR & DNA Cleanup Kit 5µg; New England BioLabs) and sequenced from both sides with Eurofins Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... After depleting ribosomal RNA (rRNA) using NEBNext rRNA Depletion Kit (New England Biolabs #E6350L), RNA was reversely transcribed using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs #E7765S ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were assembled using Gibson assembly (NEBuilder HiFi DNA Assembly Cloning Kit, NEB E5520S). Fragments were either PCR amplified with a least 15 bp overlap at each end (Phusion Hot Start Flex NEB M0535L ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR was performed on plasmid DNA with Phusion High Fidelity PCR Kit (NEB). PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB ...
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bioRxiv - Molecular Biology 2019Quote: All MKRN1 mutant plasmids were generated with the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2019Quote: ... All cloning was done using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB #E5520). PCRs were done using Q5® High-Fidelity DNA Polymerase (NEB #M0491).
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bioRxiv - Microbiology 2019Quote: ... Libraries were constructed using the NEBNext Ultra DNA Sample Prep Master Mix Kit (NEB), with minor modifications and a custom automated protocol on a Biomek FX (Beckman) ...
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bioRxiv - Genetics 2019Quote: The homologous repair constructs were created using the NEBuilder HiFi DNA Kit (NEB, E5520S) and contained 1,000 bases upstream of the first guide RNA target Cas9 site ...
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bioRxiv - Biophysics 2019Quote: ... Mutant constructs were generated using a site-directed mutagenesis kit (Q5, New England Biolabs) using primers described in the supplement ...
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bioRxiv - Biochemistry 2019Quote: We introduced mutations in phAPOBEC3B-HA using the Q5 Site-Directed Mutagenesis Kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We performed cDNA synthesis following standard protocols with the ProtoScript cDNA synthesis kit (NEB) using 1 µg of RNA for each sample ...
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bioRxiv - Genetics 2020Quote: ... cDNA was generated using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs). RT-qPCRs were performed on the Light Cycler 480II (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... The NEBNext Poly(A) mRNA magnetic isolation kit (New England Biolabs, Ipswich MA, E7490) was used to purify mRNA from the total RNA ...
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bioRxiv - Microbiology 2019Quote: ... For eight samples the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) and NextSeq (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... and mutations were made using the Q5 Site-directed mutagenesis kit (New England Biolabs). The BacMam vector was a kind gift from Professor Eric Gouaux (Vollum Institute).42 Briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... and used for cDNA library generation with the NEBNext Ultra kit (New England BioLabs), without enrichment ...
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bioRxiv - Cell Biology 2020Quote: ... using the Q5 quick mutagenesis kit (purchased from New England BioLabs, catalog number E0554s) and following the protocol provided by the manufacturer ...
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bioRxiv - Synthetic Biology 2021Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB cat# E7760L). 500 ng RNA was used at input ...
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bioRxiv - Genomics 2021Quote: ➢ Distinct from currently used length 8 barcodes from common library kits (Illumina TruSeq, NEB)
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was generated using Protoscript II First Strand cDNA Synthesis kit (New England Biolabs). cDNAs were diluted 1:20 and then qPCR was carried out in triplicate using the Luna Universal qPCR Master Mix (New England Biolabs) ...
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bioRxiv - Immunology 2021Quote: ... Libraries were then constructed with the NEB Next Ultra II FS kit (E6177S, NEB) using the prime-seq specifications ...
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bioRxiv - Microbiology 2022Quote: ... Then Trizol was added before RNA was extracted using Monarch RNA Cleanup Kit (NEB), skipping the gDNA cleaning column steps ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA libraries were prepared using the NEBNext Ultra II DNA Library Prep kit (NEB) with the NEBNext Poly(A ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted using 5x107 cells and Monarch Total RNA Miniprep Kit (NEB) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... purified from 1.5% agarose and quantified with NEBNext Library Quant Kit for Illumina (NEB). Sequencing was performed on Illumina HiSeqV4 SR50.
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bioRxiv - Synthetic Biology 2021Quote: Total RNA was extracted from cell-free expression reactions with a kit (NEB #T2010), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product was purified using the Monarch PCR & DNA clean-up kit (NEB). All library preparation and sequencing was carried out at the Vanderbilt Technologies for Advanced Genomics (VANTAGE ...
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bioRxiv - Microbiology 2021Quote: ... Mutagenesis was performed in two steps using Q5 site-directed mutagenesis kit (NEB, USA) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: The pIGLR-2∷mCherry construct was generated with a Gibson assembly cloning kit (NEB) with the following four fragments ...
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bioRxiv - Genomics 2021Quote: ... Single-nucleotide point mutations were generated using a Q5 site-directed mutagenesis kit (NEB) and confirmed by Sanger sequencing ...
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bioRxiv - Biophysics 2020Quote: ... Mutagenesis of STEAP construct was performed using the Q5 Site-Directed Mutagenesis Kit (NEB). The primers used in this study are listed in Table S1 ...
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bioRxiv - Genomics 2020Quote: ... and end-repaired through NEBnext ultra II end repair kit (New England Biolabs, USA). Next ...
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bioRxiv - Microbiology 2021Quote: ... Fragmented DNA was end-repaired using the NEBNext Ultra I kit (New England Biolabs). The fragments were then ligated with an adapter ...
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bioRxiv - Biophysics 2021Quote: ... Mutants were generated by mutagenesis PCR using the Q5 Site-Directed Mutagenesis Kit (NEB), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: Complementation vectors were generated using NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Primers were designed using the online NEBuilder Assembly Tool with the following setting ...
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bioRxiv - Molecular Biology 2021Quote: ... The capped mRNAs were in vitro synthesized with HiScribe T7 ARCA mRNA Kit (NEB) and purified with an RNA Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: ... IVT was performed using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). Nuclease prime editor mRNA and pegRNA were purified using RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... Gel fragments of homozygous clones were extracted using Monarch Nucleic Acid Purification Kits (NEB) and submitted for Sanger Sequencing ...
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bioRxiv - Immunology 2020Quote: ... and gel fragments of clones were extracted using Monarch Nucleic Acid Purification Kits (NEB) and submitted for Sanger Sequencing ...
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bioRxiv - Microbiology 2020Quote: ... Derivatives of this plasmid were made using a Q5 site-directed mutagenesis kit (NEB). Single copy deaD-lacZ fusions were made by recombining the plasmid-borne fusions with modified λ phage ...
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bioRxiv - Cell Biology 2021Quote: ... The fragments were assembled using NEBuilder HiFi DNA Assembly Cloning kit (New England Biolabs) to yield the pPB-TRE Flag-wtATM plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... or if Gibson assembly was used with Gibson assembly kit #E2611 (New England Biolabs). Details on the construction of the plasmids used to create the different transgenes are shown in suppl ...
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bioRxiv - Genetics 2019Quote: ... total RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were prepared using the NEBNext Ultra DNA Library Prep kit (NEB, E7370) according to manufacturer’s instructions and submitted for paired-end Illumina sequencing on the HiSeq 2500.
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bioRxiv - Genomics 2021Quote: ... then libraries were prepared using the NEBNext DNA library prep kit (New England Biolabs). Final libraries were sequenced on a Novaseq platform (Illumina) ...
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bioRxiv - Genomics 2020Quote: ... Libraries were constructed using the NEBNext Ultra DNA Sample Prep Master Mix Kit (NEB) with minor modifications and a custom automated protocol on a Biomek FX (Beckman)25 ...