Labshake search
Citations for New England Biolabs :
2851 - 2900 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... PCR amplification was performed with Q5 High-Fidelity DNA Polymerase (NEB M0491S). Following DPN1 digestion ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were processed according to the Exo-SAP protocol (NEB) and Sanger-sequenced by Azenta Life Services GeneWiz with hisC-ins-f as sequencing primer ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: PCRs were performed using the Phusion DNA Polymerase (New England Biolabs, #M0530S) following manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... The PCR product was incubated with restriction enzyme Fnu4HI (NEB cat. R0178) for >4hrs at 37C and run on a 3% gel ...
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bioRxiv - Physiology 2024Quote: ... We digested the PCR products with AgeI and NotI (New England Biolabs) and cloned them into the first position of the bicistronic vector pCAG-[first position]-2A-GFP ...
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bioRxiv - Genetics 2024Quote: ... PCR reactions were performed with Q5 High-Fidelity DNA Polymerase (NEB, UK). Genomic integration of both constructs were confirmed through PCR and Sanger sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... and amplified using NEBNext® High-Fidelity 2X PCR Master Mix (NEB). The number of cycles was estimated by qPCR ...
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bioRxiv - Cell Biology 2024Quote: For PCR reaction: Taq 2x Master Mix (New England Biolabs Inc, M0270L).
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bioRxiv - Genetics 2024Quote: ... Quantitative PCR was performed using Luna Universal qPCR Master Mix (NEB, M3003L) and a Roche LightCycler 96 machine (Roche) ...
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bioRxiv - Genomics 2024Quote: ... PCR amplification was performed using LongAmp Taq 2X Master Mix (NEB, M0287S) and the Nanopore_T7_IVT_Forward and Reverse primers ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was treated with Exonuclease I (NEB, Ipswich, MA, USA) for 15 min at 37 °C ...
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bioRxiv - Genetics 2024Quote: ... the left and right homology arms were amplified by pfusion PCR (NEB) using y w genomic DNA as template and primer pairs Ti897/898 and Ti899/900 ...
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bioRxiv - Biochemistry 2024Quote: ... and digested PCR product by NdeI and XhoI restriction enzymes (NEB, USA) was ligated using T4 DNA ligase (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Alice Ting’s plasmid (30) via PCR using Q5 high-fidelity (M0492, NEB). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... individual PCR amplicons were produced using Q5 high fidelity polymerase (NEB # M0491S) and isolated from a 0.5X TBE gel by centrifugation of a gel core through an EconoSpin column (Epoch Life Science ...
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bioRxiv - Biochemistry 2024Quote: PCR inserts were amplified with Phusion High-Fidelity DNA Polymerase (M0530L, NEB). Amplification primers were designed with a 30 bp overlap with the linear ends of restriction-digested vectors ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR reactions were performed with the Phusion High Fidelity DNA polymerase (NEB) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed using Luna Universal qPCR Master Mix (M3003, NEB) running on the Light Cycler 96 System (Roche) ...
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bioRxiv - Biochemistry 2024Quote: ... 50 µL of PCR product was digested by 1μL of DpnI (NEB) for 2 hours ...
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bioRxiv - Bioengineering 2024Quote: ... These PCR products were digested with ExoCIP (New England Biolabs, Catalog # E1050L) and used as template for a second ...
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bioRxiv - Biochemistry 2024Quote: ... which was performed with the NEBNext Universal PCR Primer for Illumina (NEB) and indexed primers (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... PCR fragments were ligased by HiFi DNA Assembly Master Mix (NEB, E2621L) and into the pCAG-Puro vector by XhoI enzyme site ...
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bioRxiv - Biochemistry 2024Quote: All PCRs were performed with the Q5 High-Fidelity DNA Polymerase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein-coding sequences for cDNA over-expression or co-immunoprecipitation were cloned into pLenti_CMV_cDNA_Flag_SV40_mCherry vector or pLenti_CMV_cDNA_HA_SV40_EGFP vector by PCR and Gibson assembly (NEB, #E2611L). All plasmids were verified by Sanger sequencing.
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bioRxiv - Microbiology 2024Quote: ... the ahpC gene was amplified by PCR with Phusion DNA polymerase (NEB) using C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 25 µL 2X NEBNext high-fidelity PCR master mix (NEB, M0541). To reduce GC/size bias and over amplification of libraries ...
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bioRxiv - Microbiology 2024Quote: ... PCR was performed using Q5 high-fidelity DNA polymerase (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... All PCRs were performed with Q5 High-Fidelity DNA Polymerase (NEB, M0491S) and digested with DpnI (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... PCR was performed with Q5 Hot Start High-Fidelity Master Mix (NEB) and purified with Monarch PCR cleanup kit (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was passed three times over a column of 10 ml of amylose resin (New England Biolabs, E8031L). The column was washed with MBP wash buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... 6 μM kifunensine was added to the ExpiCHO culture media at the time of transfection and Endo Hf (NEB) treatment of LC-kappa purified protein (∼10,000 U/mg protein at 4°C for 3 hours ...
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bioRxiv - Microbiology 2021Quote: ... Frozen pellets from the time course sampling were normalized by OD600 for resuspension in 1x blue loading buffer (NEB) supplemented with 1x reducing agent (DTT) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were spun down and washed three times in ice cold PBS and the cells incubated with DpnI (NEB) to remove any residual plasmid contamination ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction mix was incubated at 37°C and 15 ul aliquots were removed at indicated time points, quenched into stop buffer (1.8% SDS, 10 mM EDTA) followed by Proteinase-K (2 units total) (NEB) treatment for 45 min at 50°C ...
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bioRxiv - Microbiology 2023Quote: ... one primer pair (cDNA template, and nuclease-free water. The thermal cycle of the RT-qPCR machine was set up based on NEB #M3003 instruction manual (NEB, UK). Transcription values (Ct ...
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bioRxiv - Microbiology 2023Quote: ... Monarch kit (NEB) extracted and 0.1 pmol of each DNA fragment was CPER amplified in a 50 µL reaction containing 200 µM of dNTPs ...
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bioRxiv - Plant Biology 2020Quote: ... PCR was preformed using Taq DNA Polymerase (New England Biolabs, Beverly, MA, USA) as follows ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCRs were performed with Q5 Hot Start High Fidelity Polymerase (New England Biolabs) or Invitrogen Platinum Superfi II DNA Polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Diluted genomic DNA was utilized for PCR analysis using Taq DNA polymerase (NEB) and genotyping primers indicated in Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... and PCR-amplified with Q5 Hotstart high-fidelity 2X mastermix (NEB, Cat #M0494) and barcoding primers (Table S7 ...
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bioRxiv - Cell Biology 2020Quote: ... The gRNA-locus is then PCR amplified with OneTaq DNA polymerase (NEB, M0480) using 1 µg of genomic DNA ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were set up using the Q5 DNA polymerase (New England Biolabs) according to manufacturer’s instructions and the following primer pairs:
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bioRxiv - Cell Biology 2020Quote: PCR was conducted using Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491) according to manufacturer protocol with 400-800ng template DNA ...
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bioRxiv - Genetics 2021Quote: ... The colony PCR amplified products were digested with AvrII (New England Biolabs R0174S) in CutSmart buffer at 37°C for 3 hours before being resolved with agarose gel electrophoresis.
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bioRxiv - Developmental Biology 2021Quote: ... 200 ng of PCR products in 1X NEB buffer 2 (New England Biolabs) were hybridized under the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... parahaemolyticus RIMD2210633 genome via Phusion High-Fidelity (HF) polymerase PCR (New England Biolabs). The amplified 670-bp opaR coding region and pBAD33 empty vector (pBADEV ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541) with the following conditions ...
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bioRxiv - Genomics 2022Quote: ... and PCR was performed using enrichment primers and Phusion HF Master Mix (NEB) for 12 cycles ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR was completed using the Luna polymerase master mix (NEB, cat# M3003) using gene-specific primers (Table S1) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR across the 70-bp repeats were carried out with LongAmp enzyme (NEB) according to standard protocols with primers PseudoF and PACR or RFPF and VSG221R.