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Citations for New England Biolabs :
2801 - 2850 of 6227 citations for 1 1' 3' 1'' Terphenyl 4 4'' dimethanamine 5' 4 aminomethyl phenyl 9CI since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 0.1 μM DNA annealed oligos and 1 unit/μL T7 RNA polymerase (New England Biolabs). Reactions were incubated at 37°C for 5 hours ...
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bioRxiv - Genomics 2022Quote: ... and NEBNext Multiplex Oligos for Illumina Index Primers Sets 1 and 2 (NEB E7335S & E7500S). In order to increase input DNA above single library limits (1 μg ...
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bioRxiv - Genomics 2022Quote: The cDNA reaction products were treated with 20 units of Exonuclease 1 (New England Biolabs) and subsequently purified and concentrated using AMPure XP beads ...
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bioRxiv - Molecular Biology 2023Quote: ... chilled on ice and then combined with 30 U T4 RNA Ligase 1 (NEB M0437M), 40 U Murine RNase Inhibitor (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was then probed with the primary anti-MBP antibody (NEB E8032S, 1:10000) diluted in 5% NFDM (overnight ...
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bioRxiv - Biochemistry 2023Quote: Extracted samples (1 μL) were digested with NotI-HF (0.89 unit/μL, New England Biolabs) and ClaI (0.44 unit/μL ...
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bioRxiv - Cancer Biology 2023Quote: ... digested overnight at 37°C with 1 μl 20 U/μl NotI-HF (NEB R3189S) and 1 μl 10 U/μl PmeI (NEB R0560S ...
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bioRxiv - Developmental Biology 2023Quote: ... 7 μL of 2x NEBNext Library Quant Master Mix with 1:100 low ROX (NEB), and 1.5 μL ddH2O ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 1 µg RNA was reverse transcribed to cDNA (LunaScript RT SuperMix, New England BioLabs, USA).
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bioRxiv - Physiology 2023Quote: ... 1 U of Phusion® Hot Start II High-Fidelity DNA polymerase (New England BioLabs), 500 nM of the 27F-DegS primer ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg/mL of both DNase I and RNase I (New England Biolabs, Ipswich, USA) was added and incubated with the phage at room temperature for 1 hour to digest any non-phage nucleic acid present in the mix ...
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bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were ethanol-fixed and incubated with 1:100 anti-H3K27me3 antibody (NEB, 9733S) as described previously (34) ...
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bioRxiv - Genomics 2024Quote: ... amplifications were submitted to cleavage assays using the T7 Endonuclease 1 (T7E1) enzyme protocol (NEB). PCR products of confirmed G2 heterozygous (n = 48 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... adding 3.5 µL of Cutsmart buffer and 1 µL of ApaLI restriction enzyme (NEB #R0507L) to each sample’s Step 2 PCR product ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 1-2 µg phi47 DNA was treated with Nucleoside Digestion Mix (New England Biolabs) following the manufacturer’s protocol at 37°C for >2h ...
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bioRxiv - Biochemistry 2024Quote: ... EU-labeled cells fixed and permeabilized as above were incubated in 1×CutSmart buffer (NEB) with 4ng/µL RNase A or 5U/µL benzonase ...
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bioRxiv - Neuroscience 2024Quote: ... 2 ml of virus was incubated with 1 ml DNase I (NEB, 2 units/ml), 4 µL DNase I buffer ...
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bioRxiv - Microbiology 2024Quote: ... with NEBNext® Multiplex Oligos for Illumina Dual Index Primers Sets 1 and 2 (NEB). Manufacturer’s instructions were followed except the post-ligation bead clean-up ...
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bioRxiv - Immunology 2024Quote: ... PCR II mix was prepared by mixing 1 µL of 5X Q5 Reaction Buffer (NEB), 0.1 µL of dNTP Mix (10 mM each ...
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bioRxiv - Genomics 2024Quote: ... 1 μg total RNA was reverse transcribed using dT priming with Protoscript II (NEB, M0368L). qPCR was conducted using DyNAmo qPCR mastermix (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... the target site was amplified by Taq DNA polymerase:DeepVent polymerase 200:1 U (both NEB) with CRISPR efficiency primers and sequenced ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was generated from 1 µg RNA using LunaScript RT SuperMix Kit (New England Biolabs). qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... during 1 h at 37°C and ligated using T4 DNA ligase (New England Biolabs) at 25°C overnight before being used for transformation toOne Shot MAX Efficiency DH10B chemically competent cells (Invitrogen) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg of the vector backbone (pSP0769) was linearized using PmeI (NEB, Cat. No. R0560S) for 1h and gel purified.
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bioRxiv - Molecular Biology 2024Quote: ... then DNA breaks were end-repaired (1 hr room temperature; Quick Blunting Kit (NEB, E1201L) plus 100 µg/mL BSA ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining supernatant was incubated with 1 ml of amylose resin (NEB, Ipswich, Massachusetts, USA) rotating for 2 hours at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... was digested at 37°C for 1 hour with BglII and PspXI (New England BioLabs), following the manufacturer’s standard protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 25 fmol of each plasmid was added per reaction with 1 µLBsmBI-v2 (NEB #R0739), 1 µL HiT4 ligase (NEB #R2622) ...
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bioRxiv - Bioengineering 2024Quote: ... These DNA were mixed with 1 µL of T4 DNA ligase buffer (New England Biolabs), 1 µL of NEBridge Golden Gate Assembly Kit (BsmBI-v2 ...
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bioRxiv - Biochemistry 2024Quote: ... gel-purified in triplicate from a 1% gel (NEB Monarch DNA Gel Extraction Kit #T1020L), eluted in 20 μL ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 µl of the DNA polymerase I large (Klenow) fragment (NEB, cat. no. M0210L)) ...
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bioRxiv - Plant Biology 2021Quote: ... a non-adenylated 3’ adapter was ordered for chemical synthesis from IDT™ and adenylated with the 5’ DNA Adenylation Kit (New England Biolabs® Inc.) (see Supplemental Table 4 for primers and adapters used) ...
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bioRxiv - Cell Biology 2020Quote: The PAQR-2p∷PAQR-1∷GFP construct was generated with a Gibson assembly cloning kit (NEB) with the following three fragments ...
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bioRxiv - Genomics 2022Quote: ... The electroporated bacteria was immediately split into ten 1 mL aliquots of outgrowth medium (NEB, B9035S) and incubated at 37°C for 1 hr then independently scaled up in 20 mL of LB supplemented with 100 μg/mL of carbenicillin (Teknova ...
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bioRxiv - Genomics 2020Quote: ... and barcoded with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England Biolabs). We sequenced prepared libraries on an Illumina MiSeq using MiSeq Reagent Kit v3 (2×300 cycles ...
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bioRxiv - Genomics 2020Quote: ... All ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation.
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bioRxiv - Genomics 2020Quote: ... All ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation.
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μg of linearised DNA was then used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
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bioRxiv - Molecular Biology 2020Quote: ... After incubation for min at 55°C 1 μl T4 ligase (400,000 units/ml, NEB, M0202) was added ...
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bioRxiv - Genomics 2021Quote: ... 500 ng of DNA was dissolved in 9 μL of 1 × CutSmart buffer (New England Biolabs) and sheared by pipetting 30 times with a pipette set at 8 μL with a P2/P10 tip ...
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bioRxiv - Microbiology 2021Quote: ... The pGHJ-TgApiAT6-1 and pGHJ-TgApiAT1 [27] plasmids were linearized by digestion with NotI (NEB) for 2 hr ...
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bioRxiv - Bioengineering 2020Quote: A 10x Cas12-gRNA complex mixture was prepared by pre-incubating 1 μM of LbCas12a (NEB) with 1.25 μM gRNA in 1x NEBuffer 2.1 at 37°C for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (in 10.5 μl of nuclease-free water) were combined with 1 mM ATP (NEB), 40 U RNaseOUT ...
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bioRxiv - Biochemistry 2020Quote: ... the amplified DNA and the P4H-TM isoform 1 plasmid were both digested with BlpI (NEB) and XbaI (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... and 7.5 μl of 1:1330 diluted micrococcal nuclease (11.3 gel units, New England Biolabs: NEB) for 5 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... and 7.5 μl of 1:1330 diluted micrococcal nuclease (11.3 gel units, New England Biolabs: NEB) for 5 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse transcriptase (RT) ...
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bioRxiv - Cancer Biology 2020Quote: ... and ligated into pLKO.1 vectors using T4 DNA ligase and manufacturer protocols (New England Biolabs). DNA was transformed into Stbl3 bacteria for plasmid preparation (Thermo Fisher).
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bioRxiv - Cell Biology 2021Quote: ... Receptors were surface labelled by addition of Snap-Cell 647 SiR (1:1000, New England Biolabs) to the media for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of crude mitochondria were mixed with 1/10 volume gel loading dye (7025, NEB) and subjected to Semi-Denaturing Detergent Agarose Gel Electrophoresis (SDD-AGE) ...