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Citations for New England Biolabs :
2751 - 2800 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Samples were cleaned using a 50 μg Monarch RNA Cleanup Kit (New England Biolabs) and treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... we generated RNA-seq libraries using the NEBNext Rrna Depletion Kit (New England Biolabs) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina kit (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA was synthesized using the LunaScript™ RT SuperMix Kit (New England Biolabs) in 20 μL reaction containing 4 μL SuperMix (5X ...
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bioRxiv - Immunology 2024Quote: ... and NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Mutation of ORF2 RT domain was introduced using Q5 site-directed mutagenesis kit (NEB). Deletion of ORF2 EN domain was performed by PCR and ligation cloning.
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using the LUNA 1-step RT-qPCR kit (E3005L, NEB) on an AriaMx Real-time PCR system (G8830A ...
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bioRxiv - Molecular Biology 2024Quote: ... the NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs, E7645L) was used as described previously (56 ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified using the Monarch PCR and DNA Cleanup Kit (NEB, #T1030L) prior to Sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) and qPCR performed using iTaq Universal Probes Kit (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... coli and catP of pMTL83151 were ligated using Gibson Assembly kit (New England Biolabs) (Fig ...
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bioRxiv - Microbiology 2024Quote: ... ligation-based library preparation was performed using a NEBNext Ultra II DNA Kit (NEB). The library was then subjected to Illumina NovaSeq paired-end sequencing (150-bp).
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bioRxiv - Immunology 2024Quote: ... transcribed in vitro using HiScribe™ T7 High Yield RNA Synthesis Kit (#E2040S, NEB) and purified with RNA Clean & Concentrator™ (#R1017 ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were prepared using NEBNext UltraTMII DNA Library Prep Kit for Illumina (NEB, E7645) following manufacturers instructions ...
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bioRxiv - Microbiology 2020Quote: ... glabrata total cell lysates using the anti-Rad53 antibodies described in the previous section and Protein A magnetic beads (New England Biolabs). The immunoprecipitated samples were run on 8% acrylamide gels ...
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bioRxiv - Cell Biology 2022Quote: SDS-PAGE and Western blot analysis were performed as previously described (Alpy et al, 2005) using the following antibodies: rabbit anti-GFP (1:2000; TP401, Torrey Pine Biolabs), rabbit anti-MOSPD2 (1:250 ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies used were: Alexa-680 goat anti-mouse IgG and Alexa-800 goat anti-rabbit IgG (New England Biolabs). For DRGN cultures ...
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bioRxiv - Neuroscience 2021Quote: ... was separated into low-protein absorption tubes (PROKEEP; Watson Bio Lab, Tokyo, Japan) and reacted with 2 μg of rabbit polyclonal anti-lactyllysine antibody (PTM Biolabs) or normal rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Microbiology 2021Quote: ... and either stored at −20°C or 10 μg used for immunoblots with an Anti-IkBa antibody (New England Biolabs) (54).
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bioRxiv - Cell Biology 2021Quote: ... Samples were resolved by SDS-PAGE in urea loading buffer and analysed by Western blot against the SNAP-tag (rabbit polyclonal anti-SNAP-tag antibody P9310S, New England Biolabs) to detect levels of SNAP-GLP-1R in the different fractions ...
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bioRxiv - Biochemistry 2020Quote: ... pCSE2.6-hFc-XP or pCSE2.6-mFc-XP 68 where the respective single chain variable fragment of the antibodies or antigens were inserted by NcoI/NotI (NEB Biolabs) digestion ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were separated through SDS-PAGE and transferred to PVDF membrane followed by incubation with anti-MBP monoclonal antibody (E8032S, NEB) and M2 Flag antibody (A8592 ...
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bioRxiv - Immunology 2021Quote: The cDNA sequences of the paired variable heavy and light chain region of anti-RBD antibody clones were synthesized as gBlocks (IDT) and cloned by the Gibson assembly (NEB) into human IgG1 heavy chain and light chain expression plasmids ...
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bioRxiv - Neuroscience 2020Quote: ... + 5 % non-fat milk for 1 hour at room temperature before incubating with primary antibody: anti-GFP (1:1000, Biolabs) or anti-actin (1:5000 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed three times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (anti-rabbit IgG HRP, 1:5000 dilution, 7074S, NEB) in TBS-T with 1% (w/v ...
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bioRxiv - Microbiology 2023Quote: Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
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bioRxiv - Bioengineering 2024Quote: Full length heavy and light chains for each antibody were cloned by restriction enzyme digest or Gibson Assembly (New England Biolabs) into pCMVR either individually or with a linker ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred onto a PVDF membrane and corresponding SNAP/FLAG-hGLP-1R levels detected by Western blotting with an anti-SNAP antibody (P9310S, New England Biolabs). Data was represented as amount of bound Photo-Click cholesterol relative to SNAP/FLAG-hGLP-1R.
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated through SDS-PAGE and transferred to a PVDF membrane followed by incubation with anti-MBP monoclonal antibody (E8032S, NEB) and M2 Flag antibody (A8592 ...
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bioRxiv - Plant Biology 2020Quote: ... Sequencing libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA), and the quality was assessed using the Agilent 2100 Bioanalyzer ...
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bioRxiv - Plant Biology 2020Quote: ... Stranded libraries were prepared using the NEBNext Ultra Directional RNA Library Kit (New England Biolabs), barcoded ...
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bioRxiv - Microbiology 2020Quote: Fetuin was deglycosylated under denaturing conditions using Protein Deglycosylation Mix II kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... sgRNA was in vitro transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). Samples were incubated at 37°C for 16 hours before purifying the sgRNA with Invitrogen TRIzol Reagent (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The cDNA was purified using the Monarch DNA and PCR Cleanup Kit (New England Biolabs). A region around an A-to-I editing site in BPNT1 was amplified by Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs ...
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Chromatin accessibility changes at intergenic regions associates with ovarian cancer drug resistancebioRxiv - Cancer Biology 2021Quote: Libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized with the First Strand cDNA Synthesis Kit (New England BioLabs, Ipswich, MA) and analyzed for expression of collagen I (COL1A1 ...
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bioRxiv - Physiology 2021Quote: ... Libraries were quantified with quantitative PCR (NEBnext Library quantification kit, New England Biolabs, Ipswich, USA) and a Agilent Bioanalyzer High Sensitivity DNA chip ...
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bioRxiv - Cell Biology 2019Quote: ... A sequencing library was constructed with NEBNext Ultr II RNA Library Prep Kit (NEB, E7770S) and amplified with NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... DNA concentration were measured using the NEBNext® Library Quant Kit for Illumina (E7630L, NEB). Each 50 μl PCR reaction was composed of 0.5 μM primers ...
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bioRxiv - Genetics 2021Quote: ... sgRNAs or crRNAs were transcribed using the HiScribe T7 High Yield RNA Synthesis kit (NEB) or the MEGAscript T7 Transcription kit (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2021Quote: The DNA template oligo was amplified using the Phusion DNA polymerase kit (NEB, cat# M0530S). Refer Table S1 for primer sequences ...
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bioRxiv - Microbiology 2021Quote: ... libraries were prepared using a NEB ultra II DNA Library Prep Kit for Illumina (NEB), quantified using a Kapa qPCR Library Kit (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... The capped mRNAs were in vitro synthesized with the HiScribe T7 ARCA mRNA Kit (NEB) and purified with an RNA Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Developmental Biology 2021Quote: mRNA was synthesized using the HiScribe™ T7 ARCA mRNA Kit (with tailing) (NEB, E2060S) and a PCR product amplified from the Kaede-NLS plasmid (addgene ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and constructed sequencing libraries with the NEBNext Ultra II RNA Library Prep kit (NEB #E7770) using dual indices (NEB #E7600) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Final concentration was measured by qPCR using the NEBNext Library Quant Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The repair template was cloned using the Gibson assembly kit (New England Biolabs, MA, USA). Suitable target sites without off-targets were identified using the CRISPR Optimal Target Finder (134 ...
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bioRxiv - Developmental Biology 2020Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... 300ng of DNA were used for the library preparation using the Ultra II kit (NEB) for adaptation ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... Alternate allele plasmids were generated via site directed mutagenesis (Q5 SDM kit, New England Biolabs) using the following primers ...