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Citations for New England Biolabs :
2651 - 2700 of 10000+ citations for Mouse Tyrosinase Related Protein 1 TYRP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2020Quote: ... Briefly: agarose plugs were pre-incubated for 1 hour with 1× Cutsmart buffer (New England Biolabs), followed by overnight incubation with 20U restriction enzymes HinfI and 20U RsaI (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... The plug was then equilibrated twice in 1 ml of 1× NEBuffer 3.1 (New England Biolabs) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... Reagents added were 1 μl of 1 mg/ml BSA (diluted from 20 mg/ml - NEB), 1 μl T4 DNA ligase buffer (NEB or Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then 0.3 μL of 1 mg mL−1 trypsin (trypsin-ultra, MS-grade, New England Biolabs) was added and samples were incubated at 37 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... and used in a 1:1 molar ratio Golden Gate assembly reaction with BsaI-HF2 (NEB) and T4 ligase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Filtered (0.45 µm) supernatants were treated with 1 U/ml DNAse I (NEB; 1 h, RT) and purified through a 20% sucrose cushion (2 h ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μL T4 DNA ligase (400 U/μl) and 1 μl BsaI-HFv2 (M0202S, R3733S, NEB) and water to 15 μl ...
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bioRxiv - Neuroscience 2023Quote: ... The oligonucleotides were mixed at a 1:1 ratio and phosphorylated with T4 PNK (NEB, M0201) and annealed in T4 ligase buffer (NEB ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Plugs were washed in 500 µl of 1× T4 polymerase buffer (1× T4 ligase buffer (NEB) supplemented with 100 μg/ml BSA and 100 μM dNTPs (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... and digested by proteinase K (New England Biolabs, 1:100 dilution to 8 units ml−1) overnight at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Reagents added were 1 μl of 1 mg/ml BSA (diluted from 20 mg/ml - NEB), 1 μl T4 DNA ligase buffer (ThermoFisher/NEB) ...
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bioRxiv - Genetics 2020Quote: For Southern blot analysis 150 ng of mouse DNA was digested with 10 units of BamHI-HF (NEB, Figure 2 and 5), NdeI (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of genomic DNA were digested and loaded into each lane. Mouse (M. musculus and M. spretus) genomic DNA was digested with methylation-sensitive (HpyCH4IV, NEB cat. R0619L) or methylation-insensitive (ApoI ...
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bioRxiv - Genomics 2024Quote: ... 120 μg of total liver RNA was isolated from 60-100 mg liver tissue from each HDI-STARR-seq-treated mouse by TRIzol guanidinium thiocyanate-phenol-chloroform extraction then purified by polyA selection using oligo(dT) beads (New England Biolabs, cat. #E7490L). The purified RNA was digested with Turbo DNase-I (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... A pre-cleaning step was applied by incubating each sample with 10 μl of Protein A magnetic beads (New England Biolabs GmbH, Frankfurt, Germany) for 1 h at 4 °C in tube roller (15 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... The DNA encoding the EGFP-fusion proteins and the P2A peptide was inserted by Gibson assembly using NEBuilder HiFi DNA assembly master mix (New England BioLabs, Ipswich, MA, USA). The HeLa Flp-In T-REx K44A Dynamin2-EGFP-P2A-Caveolin1-mCherry construct was obtained by in vitro mutagenesis of the pcDNA/FRT/TO/Dyn2-EGFP-P2A-Caveolin1-mCherry construct exchanging lysine at position 44 to an alanin using these primers ...
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bioRxiv - Developmental Biology 2020Quote: ... A total of 863 Bicyclus embryos were injected with 300 ng/µl of guide and 300 ng/µl of Cas9 protein (NEB; Cat. no.: M0641) mixed together in equal parts (total volume = 10 µl ...
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bioRxiv - Developmental Biology 2019Quote: ... The bound protein (~42kDa, 360 amino acids) was released from the MBP tag by cleavage with Factor Xa (New England Biolabs, Massachusetts, United States) for 24 hours at 4°C in 20mMTris pH 7.2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The synthetic genes encoding WT human TRIM28 with an N-terminal maltose binding protein (MBP) affinity tag (UniProt: Q13263) were subcloned to the pMAL-p2X vector (New England Biolabs, Beverly, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... corresponding to a portion of PfRNF1 (RP2; Fig. 1A) was expressed as a maltose-binding fusion protein using the pMAL™c5X-vector (New England Biolabs, Ipswich, USA). To this end ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1 µL T4 PNK (NEB) followed by incubation for 60 min at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μl (10U) T7 endonuclease (NEB) were added to the hybridized PCR products and incubated for 15min at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µl rSAP (NEB, Cat M0371) was then added to dephosphorylate 3’ end of DNA at 37℃ for 1hr ...
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bioRxiv - Microbiology 2022Quote: ... and 1 µl 5’deadenylase (NEB) at 30°C for 30 min and removed by RNA Clean & Concentrator (Zymo Research) ...
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bioRxiv - Microbiology 2022Quote: ... 1 µl of rSAP enzyme (NEB) and 1 µl of PNK enzyme (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 x protease inhibitor cocktail (NEB), 2 mM N-ethylmaleimide (NEM) ...
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bioRxiv - Molecular Biology 2021Quote: ... in 1X NEBuffer 1 (NEB #B7001) for 15 min at 37°C while shaking at 1000 rpm for an interval of 15 sec every 3 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl β-agarase (NEB M0392S) was added and mixed by flicking without allowing sample to cool ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl β-agarase (NEB M0392S) was added and mixed by flicking without allowing sample to cool ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μL murine RNase inhibitor (NEB), and 1 μL of 200 U/μL SuperScript III reverse transcriptase (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: ... 1 µl 10x Taq buffer (NEB), 0.2 µl 10 mM dNTPs (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µl 10x Vent buffer (NEB), 0.2 µl 10 mM dNTPs (NEB) ...
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bioRxiv - Genomics 2022Quote: ... 1 μl rSAP enzyme (NEB, M0371L) and 1 μl PNK enzyme (NEB ...
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bioRxiv - Genomics 2022Quote: ... added 1 μl RecJf (NEB, M0331) and 1 μl 5’deadenylase (NEB ...
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bioRxiv - Genomics 2020Quote: ... we added 1 μlExonuclease I (NEB), 1 μl Exonuclease III (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... HiFi Polymerase (1 unit, Phusion, NEB) in GC buffer with MgCl2 (NEB ...
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bioRxiv - Genomics 2020Quote: ... 1 μl T4 DNA Polymerase (NEB) and 5 μl Large (Klenow ...
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bioRxiv - Genomics 2020Quote: ... in 1X NEBuffer 1 (NEB #B7001) at 37°C for 15 minutes ...
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Genetic mapping of fitness determinants across the malaria parasite Plasmodium falciparum life cyclebioRxiv - Evolutionary Biology 2019Quote: ... 1 mM dNTPs (New England Biolabs), 3.5 μM of each amplification primer (Oyola et al ...
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bioRxiv - Molecular Biology 2019Quote: ... or T4 RNA-ligase 1 (NEB), respectively ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 U of Taq polymerase (NEB), 0.25 μM forward primer ...
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bioRxiv - Genomics 2019Quote: ... 1 μl T7e1 enzyme (NEB, USA) was added and incubated at 37°C in a water bath for 15 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 µL T4 Ligase Buffer (NEB) and 0.5 µL BsaI (10000 U/mL ...
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bioRxiv - Genomics 2020Quote: ... 1 µL 10x Ligase Buffer (NEB) and 1 µL T4 DNA Ligase (NEB ...
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bioRxiv - Genomics 2021Quote: ... 1 mM dNTPs (New England Biolabs), 2.5 μM of each amplification primer (Additional file 1 ...
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bioRxiv - Genetics 2020Quote: ... and sodium fluoride (NEB, 1 mM). 150uL of ice cold buffer was added to heads followed by immediate homogenization with a motorized pestle for 10 seconds on ice ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM dNTPs (New England Biolabs), 6.67 mM DTT (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... 1 mM ATP (New England Biolabs), filled to 10 μL with dH2O prepared on ice ...
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bioRxiv - Bioengineering 2020Quote: ... 1 mM ATP (New England Biolabs), filled to 100 μL with dH2O prepared on ice ...
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bioRxiv - Bioengineering 2020Quote: ... 1 mM of each rNTP (NEB), and 9 mM magnesium chloride ...