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Citations for New England Biolabs :
2551 - 2600 of 9563 citations for Mouse Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were prepared using the NEBNext Ultra DNA Library Prep kit (NEB, E7370) according to manufacturer’s instructions and submitted for paired-end Illumina sequencing on the HiSeq 2500.
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bioRxiv - Genomics 2021Quote: ... then libraries were prepared using the NEBNext DNA library prep kit (New England Biolabs). Final libraries were sequenced on a Novaseq platform (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... reverse transcription was performed using the ProtoScript II First Strand cDNA Synthesis Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Libraries were constructed using the NEBNext Ultra DNA Sample Prep Master Mix Kit (NEB) with minor modifications and a custom automated protocol on a Biomek FX (Beckman)25 ...
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bioRxiv - Genetics 2020Quote: Repair template plasmids were assembled from overlapping fragments using the NEBuilder HiFi Kit (NEB) per manufacturer instructions ...
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bioRxiv - Genetics 2020Quote: ... The variant was introduced using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). Cells were transfected with 6 μg of each plasmid using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA was isolated from total RNA using a Magnetic mRNA isolation kit (S1550S, NEB), and libraries were prepared using a NEXTflex Rapid directional RNA-seq kit (5138-08 ...
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bioRxiv - Microbiology 2022Quote: ... purified using the Monarch RNA Cleanup Kit (T2050S/L, New England Biolabs, Ipswich, Massachusetts), reannealed by heating to 94°C and slowly cooling to room temperature over 50 min ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCS2-Rab11S25N-mCherry plasmid was generated using Q5 Site-Directed Mutagenesis Kit (NEB) with PCS2-Rab11-mCherry plasmid and Rab11S25N-mCherry-Fwd (TGTGGGGAAGaatAACCTGCTGT ...
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bioRxiv - Genomics 2022Quote: ... RNA was purified using the 50 μg Monarch® RNA Cleanup Kit (T2040S, NEB). The concentration of RNA was measured using Qubit RNA BR Assay Kit (Q10210 ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated using the Monarch Total RNA Miniprep Kit (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA Library was generated using NEB Next Ultra II DNA library kit (NEB, E7645S) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were prepared using a NEBNext DNA Library prep kit (New England Biolabs) resulting in a target insert size of 250 bp as confirmed by TapeStation (Agilent) ...
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bioRxiv - Bioengineering 2022Quote: ... The IVT RNA was purified using a Monarch RNA cleanup spin column kit (NEB) and aliquoted for storage at −70°C ...
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bioRxiv - Biochemistry 2022Quote: ... The two PCR products were ligated together by the Gibson assembly cloning kit (NEB)
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bioRxiv - Bioengineering 2022Quote: RNA was isolated from gut mucosal biopsies using Monarch Total RNA Miniprep Kit (NEB) according to the manufacturers’ protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA synthesis was performed using the ProtoScript II First Strand cDNA Synthesis Kit (NEB). Expression levels of target genes were determined by RT-PCR using SensiMix SYBR No-Rox (Bioline ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted using Monarch Total RNA Miniprep Kit (New England Biolabs, T2010S) according to manufacturer’s instructions with DNase treatment (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: ... 5ng of DNA were used to build libraries using NebNext-UltraII kit (NEB, cod.E7645S). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... followed by the NEBNext Ultra Directional RNA library preparation kit for Illumina (NEB #E7420L) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly® Cloning Kit from New England Biolabs)57,58 or via QuickChange (Q5® Site-Directed Mutagenesis Kit from New England Biolabs).59 Recombinant expression of motors in E ...
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bioRxiv - Genomics 2022Quote: ... The NEBNext® Ultra DNA Library Prep kit for Illumina (cat# NEB #E7370S/L) was used to process the DNA samples ...
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bioRxiv - Genomics 2022Quote: ... Extracted RNA was fragmented using the NEBNext Magnesium RNA Fragmentation module kit (NEB, USA), heating for 2min ...
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bioRxiv - Immunology 2022Quote: ... All fragments were assembled with NEBuilder HiFi DNA Assembly Cloning Kit (E5520S, NEB, USA), transformed into DH5-α Competent E ...
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bioRxiv - Developmental Biology 2022Quote: ... or from COV434 cells and hiPSCs using the Monarch Total RNA Miniprep kit (NEB). For experiments involving TF overexpression ...
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bioRxiv - Plant Biology 2022Quote: ... was constructed using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, MA, USA) by multi-DNA fragment assembly between (i ...
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bioRxiv - Plant Biology 2022Quote: ... and was done using an NEBNext Ultra RNA Library Prep Kit for Illumina (NEB). After cluster generation on a cBot cluster generation system using Truseq PE Cluster kit (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA was depleted with NEBNext rRNA Depletion Kit for Bacteria (New England Biolabs). Barcoded libraries were made with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... The plasmids were isolated by using a Monarch Plasmid Miniprep kit (New England Biolabs) according to the manufacturer’s protocol and PCR amplified using primers MMEJ R1 and MMEJ R2R ...
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bioRxiv - Microbiology 2022Quote: Plasmid DNA was prepared using a Monarch® Plasmid Miniprep Kit (NEB, cat# T1010). Genomic DNA was prepared using the DNeasy Blood & Tissue Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was purified using the Monarch® PCR & DNA Cleanup Kit (NEB, cat# T1030). PCR was performed using either KAPA HIFI 2X ready mix (KAPA Biosystem ...
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bioRxiv - Microbiology 2022Quote: ... Bt1760 and Bt1761 variants were generated using the Q5 site directed mutagenesis kit (NEB). All constructs were verified by Sanger sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... Library preparation was carried out using the NEBNext Small RNA Library kit (NEB, UK), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... RNA was fragmented using the NEBnext mRNA first and second strand synthesis kits (NEB). One to three biological replicates were generated for each condition and on average ~10 million reads were generated per sample.
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bioRxiv - Molecular Biology 2022Quote: ... with the NEBNext® Library Quant Kit for Illumina® (New England Biolabs, UK) and diluted to 1 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... high yield in vitro transcription (HiScribe T7 Quick High Yield RNA Synthesis Kit, NEB), and cDNA synthesis reactions (Maxima H Minus Reverse Transcriptase ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ΔDEP Venus-DVL2 was cloned with the Q5 Site-Directed Mutagenesis kit (NEB #E0554S) using Venus-DVL2 as a template ...
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bioRxiv - Neuroscience 2022Quote: Library was prepared using the NEBNext Ultra Library prep Kit (E7645S, New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the template was used for in vitro transcription using EnGen sgRNA synthesis Kit (NEB), and the reaction was cleaned up using the MEGACLEAR Transcription Clean-Up KIT (Thermo) ...
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bioRxiv - Neuroscience 2022Quote: ... A NEBNext® Single Cell/Low Input RNA Library Prep Kit (New England Biolabs) was used for library generation ...
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bioRxiv - Neuroscience 2022Quote: ... and mPer2W419E (Fig S2) were generated using Q5 Site-Directed Mutagenesis Kit (NEB E0554S). pcDNA-mPer1and mPer2 plasmids were used as templates and have been described previously37 ...
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bioRxiv - Molecular Biology 2022Quote: ... using either the Gibson Assembly Cloning Kit (New England Biolabs, Ipswich, MA, United States) or in-Fusion Cloning Kit ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by cleaning up with Monarch PCR & DNA Cleanup Kit (T1030, New England Biolabs). The ligated DNA was repaired with PreCR Repair Mix (M0309 ...
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bioRxiv - Plant Biology 2022Quote: ... using NEBuilder® HiFi DNA Assembly Cloning kit (New England Biolabs, Ipswich, MA, USA). For LTPG1 and LTPG2 ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR product obtained were purified using the Monarch DNA Gel Extraction Kit (NEB) and cloned into the pDONR207 using a BP Clonase II Kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: m6A RNA-IP enrichment was performed using kit manufacturer protocol (New England BioLabs, #E1610S). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... treated with phenol-free RNA Binding Buffer (included in RNA Cleanup Kits from NEB) and Proteinase K ...
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bioRxiv - Molecular Biology 2022Quote: ... and reverse transcribed using the ProtoScript® First Strand cDNA Synthesis Kit (NEB, UK) according to the manufacturer’s instructions ...