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Citations for New England Biolabs :
2501 - 2550 of 9843 citations for Swine IL 4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Removal of this codon by site-directed mutagenesis (Q5 kit, New England Biolabs) yielded the WIL-V-Chis ...
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bioRxiv - Plant Biology 2023Quote: ... followed by purification with the Monarch RNA Clean Up Kit (NEB, Frankfurt, Germany). gDNA removal was assessed by PCR on chloroplast DNA followed by agarose gel electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... ribosome footprints were purified using the Monarch RNA cleanup kit (NEB cat# T2030) following the modified protocol for small RNA.
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bioRxiv - Biochemistry 2023Quote: ... The deletion was done by Gibson assembly using the Gibson Assembly Kit (NEB). The restriction enzyme ClaI (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... and plasmid DNA extracted using the Monarch Plasmid Miniprep Kit (New England Biolabs), and Sanger sequencing used to confirm correct insertion of coding sequences at the Ramaciotti Centre for Genomics (UNSW Sydney) ...
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bioRxiv - Biochemistry 2023Quote: Cas12a mutants were cloned using Q5 polymerase and KLD kit (New England Biolabs) and purified the same way as WT AsCas12a.
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bioRxiv - Synthetic Biology 2023Quote: ... and plasmids were extracted using the Monarch Plasmid Miniprep Kit (New England Biolabs). Both pEndo and pTarget were transformed into E ...
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bioRxiv - Molecular Biology 2023Quote: ... The libraries were then purified using the Monarch PCR & DNA Cleanup Kit (NEB), using a 5:1 ratio of Binding Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the plasmid above was modified using the Q5 Site-Directed Mutagenesis Kit (NEB) along with the following primers ...
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bioRxiv - Genomics 2023Quote: ... The NEB UltraII kit (New England Biolabs, Ipswich, MA, USA, cat. no. E7646A) was used for end-repair and A-tailing ...
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bioRxiv - Synthetic Biology 2023Quote: ... for large rearrangements and Q5® Site-Directed Mutagenesis Kit (New England BioLabs) for small modifications i ...
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bioRxiv - Genetics 2023Quote: ... and libraries prepared using NEB Ultra II DNA Library Prep Kit (NEB, #E7645S) and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 4 ...
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bioRxiv - Plant Biology 2023Quote: ... Reactions were purified using a Monarch PCR & DNA Cleanup Kit (New England Biolabs) and sent to Source BioScience ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... RNA was extracted using TRIzol and column purification (NEB Monarch RNA cleanup kit), and samples were then individually diluted in NEBuffer 2 (NEB ...
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bioRxiv - Genomics 2023Quote: ... rRNA depletion was performed using the NEBNext® rRNA Depletion Kit (Bacteria) (NEB) and the sequencing libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The resulting DNA fragments were cloned using the Gibson Assembly Cloning Kit (NEB) into plasmid backbone pUN4 to obtain pUN-OXA48WT ...
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bioRxiv - Immunology 2023Quote: ... and NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB; Ipswich, USA). RNA-Seq was then performed at a depth of 50M reads/sample on an Illumina NovaSeq 6000 S4 instrument of 100 base-pair long paired-end sequence reads (excluding adapter/index sequences) ...
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bioRxiv - Cell Biology 2023Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB 7760) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Plasmid DNA was purified using the Monarch Plasmid DNA Miniprep Kit (NEB, #T1010), and subjected to diagnostic restriction digest and subsequent gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... Double-stranded RNA was purified using Monarch RNA Cleanup Kit (50 µg; NEB) and eluted in 30 μl of nuclease-free water ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The repair template plasmid (pKK4) was assembled using the NEBuild HiFi Kit (NEB) from a combination of a restriction digest fragment and a synthetic gBlock Gene Fragment (IDT) ...
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bioRxiv - Neuroscience 2023Quote: ... NEBNext Ultra II RNA Library Preparation Kit for Illumina (#E7770; New England Biolabs) was used ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... site-directed mutagenesis was conducted using the Q5 Site-Directed Mutagenesis Kit (NEB). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... FN2 deletion was accomplished using Q5 Site-Directed Mutagenesis kit (New England Biolabs) according to the manufacturer’s directions and in-frame deletion was verified with Sanger sequencing ...
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bioRxiv - Genetics 2023Quote: ... using the NEB Ultra II RNA seq kit (New England Biolabs, Ipswich, Massachusetts), with the PolyA selection module ...
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bioRxiv - Genetics 2023Quote: ... and mRNA was transcribed using the HiScribe T7 high yield RNA kit (NEB) using N1-methylpseudouridine (Trilink ...
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bioRxiv - Molecular Biology 2023Quote: ... In vitro transcription was performed using a T7 Quick HiScribe kit (NEB, E2050S) according to manufacturer’s instructions with overnight incubation at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... coli overnight cultures using a Monarch plasmid miniprep kit (New England Biolabs, USA) and concentration was quantified by NanoDrop (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... adaptors were ligated to the purified amplicons using the Quick Ligation Kit (NEB), and the ligation products were purified using the Agencourt AMPure XP system (Beckman Coulter) ...
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bioRxiv - Cell Biology 2023Quote: ... The NEBNext Ultra II RNA Library Prep Kit for Illumina® (NEB, #E7775) was then used to prepare libraries for paired-end (2×150 bp ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli were performed with the Monarch® Plasmid Miniprep Kit (New England Biolabs).
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bioRxiv - Evolutionary Biology 2023Quote: ... followed by column purification using Monarch Total RNA Miniprep Kit (New England Biolabs). The ProtoScript First Strand cDNA Synthesis Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... using the NEBNext UltraTM RNA Library Prep Kit for Illumina® (NEB, USA), with enrichment for mRNA using oligo(dT)-magnetic beads ...
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bioRxiv - Biochemistry 2023Quote: ... GST-tag HP1⍺ΔC construct was generated by site-direct mutagenesis kit (NEB) by deleting the amino acids (number 169 –181 ...
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bioRxiv - Biochemistry 2023Quote: ... and the NEBNext Ultra RNA library prep kit (New England Biolabs, MA, U.S.A) manufacture’s protocol followed to prepare the sample for Illumina® RNA-sequencing (RNA-seq) ...
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bioRxiv - Biochemistry 2023Quote: ... Site-directed mutagenesis was performed using the Q5 site-directed mutagenesis kit (NEB).
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Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)bioRxiv - Biochemistry 2023Quote: ... PCR fragments were processed using the NEBuilder Hifi DNA assembly kit (NEB #E2621S) using the steps described above.
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... FBXO22 mutants were generated using Q5 site-directed mutagenesis kit (New England Biolabs) following the manufacturer’s instruction.
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized using ProtoScipt First Strand cDNA Synthesis Kit (New England Biolabs) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... PCR products were gel purified with the Monarch Gel Extraction Kit (NEB, T1020).
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bioRxiv - Molecular Biology 2023Quote: ... Editing activity of α-synCas was evaluated using EnGen Mutation Detection Kit (NEB). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... IVT product was then reverse transcribed with a ProtoScript II kit (NEB, E6560S) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from cells using Monarch Total RNA Miniprep Kit (NEB # T2010S). Any remaining DNA was degraded using DNase I (Thermo Scientific # EN0521) ...
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bioRxiv - Genomics 2024Quote: ... The DNA was then purified using the Monarch PCR & DNA Cleanup Kit (NEB). The first-dimension electrophoresis was run in 0.7% agarose in 1xTBE buffer at 0.7 V/cm for 18h ...
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bioRxiv - Genomics 2024Quote: ... gDNA extraction was performed using a Monarch Genomic DNA Purification Kit (NEB, T3010S) following the Manufacturer’s protocol for cultured cells with an input of 5e6 HeLa cells ...
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bioRxiv - Developmental Biology 2024Quote: ... They generated mRNA libraries with NEB RNA Ultra kit (New England Biolabs E7770L) and oligo(dT ...
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bioRxiv - Molecular Biology 2024Quote: ... For library preparation NEBnext Ultra II DNA Library Prep Kit (New England Biolabs) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using Ultra II FS library preparation kit (New England Biolabs) and amplified with KAPA HiFi HotStart Real-time PCR using KAPA P5 and P7 primers (KAPA Biosystems).
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bioRxiv - Biochemistry 2024Quote: ... Phosphorylated samples were ligated using a T4 DNA ligase kit (New England Biolabs) and incubated overnight at 16°C ...
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bioRxiv - Biochemistry 2024Quote: ... All fragments were gel-purified using a Monarch DNA Gel Extraction Kit (NEB). To generate the full-length library insert ...