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Citations for New England Biolabs :
2501 - 2550 of 10000+ citations for Human Carboxyl Terminal PDZ Ligand Of Neuronal Nitric Oxide Synthase Protein NOS1AP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... MNase activity was quenched by addition of EGTA and samples were subsequently pre-cleared by addition of 40 µl Protein A Magnetic Beads (New England Biolabs, Ipswich, MA, United States) and rotating at 4°C for 60 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of both N-linked and O-linked glycans was performed by incubation with five different glycosidases: PNGaseF (New England Biolabs, 125 U/μg protein), O-glycosidase (New England Biolabs ...
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bioRxiv - Bioengineering 2022Quote: ... Processing the samples for proteomics was then continued using a 10 kDa filter aided sample preparation protocol previously published with minor modifications.[30] The proteins were digested using Lys-C enzyme (P8109S, New England Biolabs, MA, USA; 1:50) and then with trypsin (V5111 ...
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bioRxiv - Biophysics 2019Quote: ... The resulting product (typically 50 – 100 μL of 5 – 20 μM of protein) was mixed with an equimolar amount of SNAP-Surface 549 (New England Biolabs; 1 mM in DMSO) and incubated at room temperature for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... aliquots of the same protein extract were separated and treated with 200 U of Lambda Phage Phosphatase (#P0753S, New England Biolabs, Ipswich, Massachusetts, United States), as specified by the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... 5 μg of MBP-Rif2 or MBP-Rif2-min (see recombinant protein preparation) was incubated for 1 h at 4 °C with amylose resin (New England Biolabs, 50 μl per reaction). The resin with the immobilized MBP-Rif2 variants was washed 5 times with 1 ml wash buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: Injections into fertilized eggs for knockout experiments were done using 50ng/µl of each sgRNA independently and 80nM of NEB EnGen Spy Cas9-NLS protein (New England Biolabs France Genopole, Evry, France), pre-incubated at room-temperature for 15 minutes (Hill et al. ...
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bioRxiv - Plant Biology 2024Quote: ... when OD600 reached 0.5 for 4 h at 37 °C and then they were purified using amylose resin column (New England Biolabs, for MBP related proteins purification) or Ni-NTA agarose column (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... ChIP DNA was purified with the Monarch PCR & DNA Cleanup Kit (NEB). Libraries were prepared using ThruPLEX DNA-seq Kit (Rubicon Genomics ...
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bioRxiv - Plant Biology 2020Quote: ... were mixed for DNA assembly using the Golden Gate assembly kit (NEB).
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated using NEBNext Ultra II DNA Library Prep Kit (NEB), modified for CUT&RUN as previously described(54) ...
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bioRxiv - Cell Biology 2019Quote: ... then purified with Monarch PCR & DNA Purification Kit (New England BioLabs, T1030S) followed by T4 DNA Ligase reaction (New England BioLabs ...
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bioRxiv - Cell Biology 2020Quote: ... and the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760). The libraries were sequenced using Next-Seq with single-end ...
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bioRxiv - Cell Biology 2020Quote: ... we used the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S) and pDONR221-GEM1 to make pDONR221-GEM1(S19N) ...
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bioRxiv - Cell Biology 2020Quote: ... Ligations were completed using the Quick Ligation Kit (New England Biolabs, M2200) according to manufacture protocol ...
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bioRxiv - Immunology 2021Quote: ... Small RNA library preparation was completed using NEBNext Small RNA kit (NEB) following manufacturer’s instructions and applying the low input protocol modifications ...
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bioRxiv - Genetics 2021Quote: ... Cloning was done using Gibson Assembly® Cloning Kit (New England Biolabs). The final product was amplified as a linear construct and confirmed by Sanger sequencing ...
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bioRxiv - Genetics 2021Quote: ... and quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs). The quantified pool was hybridized at a final concentration of 2.2 pM and sequenced paired-end on the Illumina NextSeq 500 platform using a High-Output v2.5 flowcell and 2×151c read length.
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bioRxiv - Genomics 2020Quote: ... The Monarch Total RNA Miniprep kit (New England Biolabs Inc, Ipswich, MA) was used for extraction ...
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bioRxiv - Microbiology 2020Quote: ... using the HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA, USA). pBOMBL contains a modified ribosome binding site to reduce leaky expression ...
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bioRxiv - Microbiology 2019Quote: ... We prepared sequencing libraries with NEBNext Ultra DNA Library Prep kits (NEB) and sequenced them on an Illumina NextSeq with 2×150 paired end reads (mid-output run ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Digested DNA was purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... twelve-cycle PCR reactions (Phusion High Fidelity PCR kit, New England Biolabs), using primers that added Flow Cell binding sequences ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were produced using the EnGen® sgRNA Synthesis Kit (NEB, E3322S). Primers are given in Table S2.
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bioRxiv - Neuroscience 2022Quote: In vitro transcription was performed using HiScribe Quick T7 kit (NEB E2050S) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... competent cells and were purified using NEB Monarch Miniprep Kits (NEB, Canada) as per manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... After electrophoresis and gel extraction by Monarch DNA Gel Extraction Kit (NEB), the PCR product was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... In vitro transcription was performed using HiScribe Quick T7 kit (NEB E2050S) following the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2021Quote: ... using HiScribe™ T7 High yield RNA Synthesis kit (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... using the Luna Universal One-Step RT-qPCR kit (New England BioLabs) and primer sets validated in our lab (Supplemental Table 5) ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared using the NEBNext Ultra II kit (New England Biolabs, Ipswitch ...
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bioRxiv - Molecular Biology 2020Quote: This oligonucleotide was adenylated using the 5’ DNA adenylation kit (NEB, E2610S) as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... The linkers were pre-adenylated with a 5’ DNA Adenylation kit (NEB), and then used for the ligation reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq libraries were prepared with NEBNext UltraDirectional kit (New England Biolabs). Libraries were pooled and sequenced on Illumina HiSeq 2500 instrument ...
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bioRxiv - Molecular Biology 2021Quote: ... NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... digestion and then in vitro transcribed using HiScribe T7 Transcription Kit (NEB). The RNA transcripts were extracted using Trizol (Thermo ...
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bioRxiv - Microbiology 2021Quote: ... or NEBNext UltraII DNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was performed using a HiSeq2500 sequencer (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently purified with Monarch RNA Cleanup Kit (NEB, cat. No. T2040).
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bioRxiv - Neuroscience 2021Quote: ... Isolated transcripts were ligated into pEGFP-C2 using Quick Ligation Kit (NEB), cloned into DH5α ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... and minipreps were performed using the Monarch Plasmid Miniprep Kit (NEB T1010L). NEB 5-alpha Competent E ...
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bioRxiv - Microbiology 2019Quote: ... and quantified with the NEBNext Library Quant Kit Protocol (New England BioLabs). The NextSeq 500/550 High Output Kit (300 cycles ...
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bioRxiv - Genetics 2020Quote: ... the DSB ends were blunted with NEB’s Quick Blunting Kit (NEB #E1201) according to the manufacturer’s instructions in a final volume of 100 μl for 60 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... and quantified using the NEBNext Library Quant Kit for Illumina (NEB, #E7630). After pooling ...
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bioRxiv - Microbiology 2019Quote: ... or the Monarch PCR and DNA Cleanup Kit (New England Biolabs, US). After Sanger-sequencing (Eurofins ...
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bioRxiv - Molecular Biology 2019Quote: ... The linkers were pre-adenylated with a 5’ DNA Adenylation kit (NEB), and then used for the ligation reaction ...
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bioRxiv - Bioengineering 2019Quote: ... Gibson assembly used Gibson assembly cloning kit (New England Biolabs, Cat. # E5510S) as per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... ATP5I were cloned into pEGFPN1 vector using Gibson assembly cloning kit (NEB) to acquire their respective GFP-tagged versions.
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bioRxiv - Molecular Biology 2020Quote: ... LexA point mutants were made using Q5 site-directed mutagenesis kit (NEB) and confirmed by sequencing ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries from gDNA were prepared using NEBNext Ultra II FS kit (NEB) following manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2019Quote: ... Ligation of the linearized vector and annealed oligos (Quick ligation kit, NEB) and 4 ...