Labshake search
Citations for New England Biolabs :
2501 - 2550 of 3328 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... Supernatant was recovered after centrifugation (12000 xg, 4°C, 1h) and incubated with 0.002% equilibrated amylose resin (NEB) for 2h on a rotating wheel at 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Pellets were resuspended in NEB buffer prior to centrifugation at 11,500 rpm for 20 minutes at 4°C on a sucrose gradient (1.6M sucrose in NEB and 0.8M sucrose in NEB). Nuclei-containing pellets were fixed with 0.3% formaldehyde in NEB and rotated at room temperature for 10 minutes ...
-
bioRxiv - Biochemistry 2024Quote: ... The unbound fraction was then incubated for 1 h at 4 °C with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
-
bioRxiv - Genomics 2024Quote: ... NEBNext Multiplex Oligos for Illumina (Index Primer Sets 1-4) (Cat# E7335L, E7500L, E7710L, E7730L, New England Biolabs) were used for indexing during library preparation ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... PCRs were run in 50μl reactions (4 side-by-side replicates) using Q5 enzyme and buffers (NEB; M0491). Amplification was confirmed through agarose gel electrophoresis ...
-
bioRxiv - Biophysics 2021Quote: ... 1 μL of this PCR reaction was then combined with 5 μL Q5 buffer (NEB, cat#M0491S), 0.5 μL 10mM DNTP (Thermo Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... The annealed product was labeled by fill-in synthesis using 5 U Klenow exo-(New England Biolabs) and a dNTP mix containing 40 µM each of dATP ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were incubated with 2 nM HaloTag-JF549 and 5 nM SNAP-Cell® 647-SiR (NEB) before imaging.
-
bioRxiv - Molecular Biology 2020Quote: ... pGEMHE plasmids were linearized and 5’-capped mRNA was synthesised with T7 polymerase (NEB HiScribeT7 ARCA kit) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were then incubated at 45 °C for 2 hr with 5 μL of Proteinase K (NEB) in the presence of 40 mM EDTA (pH 8.0 ...
-
bioRxiv - Biophysics 2022Quote: ... The donor-labeled DNA was phosphorylated at the 5’ end using T4 Polynucleotide Kinase (New England BioLabs) for 1 hour at 37°C ...
-
bioRxiv - Genomics 2021Quote: ... 50 ng of DNA was digested using the 5-bp cutter ApeKI (New England Biolabs, Ipswich, MA) after which barcoded adapters were added by ligation with T4 ligase (New England Biolabs ...
-
bioRxiv - Genetics 2020Quote: ... We mixed 20 µg of insert or 15 µg plasmid library with 5 µL AscI (NEB R0558L), 5 µL SbfI-HF (NEB R3642L) ...
-
bioRxiv - Bioengineering 2021Quote: ... 5′ phosphorylation was performed either during synthesis (for tRNAfMet) or following synthesis using T4 Polynucleotide kinase (NEB)(for tRNALys and tRNAPhe ...
-
Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: GBL phosphate (8) was incubated with 5 units of calf intestinal alkaline phosphatase (CIP, New England Biolabs) in 50 mM HEPES buffer (pH 8.0 ...
-
bioRxiv - Cell Biology 2021Quote: ... FHIP1B or FHIP2A-coated IgG beads were labeled with 5 µM HaloTag-Alexa-660 (New England Biolabs) in the column for 10 minutes at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
-
bioRxiv - Plant Biology 2020Quote: ... Reactions conducted at UR-MAI consisted of 50 μl reactions: 5 μl 10X Standard Taq Buffer (NEB), 2.5 μL of each primer ...
-
bioRxiv - Microbiology 2020Quote: ... and then added up to 5 fmol DNA (2000:1000:1 RNA:protein:DNA ratio) and NEBuffer 3.1 (NEB) to 1X ...
-
bioRxiv - Genetics 2021Quote: ... The A-tailed PCR products were cleaned with Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB) and ligated into pGEM®-T Easy vector (Promega ...
-
bioRxiv - Biophysics 2020Quote: ... was added to the flow cell and incubated for 5 minutes followed by Nb.BsmI (0.2 U/μl) in 1x NEB 3.1 buffer (NEB) which was also incubated for 5 minutes ...
-
bioRxiv - Genomics 2021Quote: ... The cell suspension was then mixed with 5 ml ligation buffer (550 μl 10x ligation buffer (NEB), 27.5 μl 20 mg/ml BSA (NEB ...
-
bioRxiv - Biochemistry 2022Quote: ... Finale expression constructs were subcloned in NEB 5- alpha competent E.coli cells (New England Biolabs, Ipswich, US) followed by Sanger sequencing (Microsynth ...
-
bioRxiv - Biochemistry 2022Quote: ... mixing with 5 μL of stop buffer (50 mM EDTA and 2 mg/ml Proteinase K (NEB)) ...
-
bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
-
bioRxiv - Molecular Biology 2020Quote: ... 5’-phosphorylated and dA-tailed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Samples were ligated to adaptor oligos for multiplex sequencing (NEB ...
-
bioRxiv - Biochemistry 2020Quote: ... Fragmentation of 1 µg mRNA aliquots for 5 min at 94°C in 1x Fragmentation Buffer (NEB) was optimal to obtain a pool of 100-200 nt fragments ...
-
bioRxiv - Microbiology 2022Quote: ... with the 5’ monophosphates available on the treated viral RNAs using T4 RNA ligase (NEB, Ipswich, MA) for 1 h at 37°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... to digest the bottom strand of dsDNA and truncated the 5’ primer region with USER enzyme (NEB). We added 5 M NaCl 20% v/v to the oligo mix and pre-annealed by heating it at 95°C for 3 minutes ...
-
bioRxiv - Biophysics 2022Quote: ... a third primer ACTB K118TAG phosphorylated at its 5’ end and Taq DNA ligase (New England Biolabs) were added to the reaction mixture ...
-
bioRxiv - Biochemistry 2020Quote: ... 5 μL of plasma was lyophilized and resuspended in 20 μL 1X Rapid PNGaseF buffer (NEB #P0710S) and incubated for 15 minutes at 70°C to denature proteins ...
-
bioRxiv - Cell Biology 2020Quote: ... An 8.9 kb fragment preceding and including exon 5 was amplified by PCR (Phusion DNA Polymerase, NEB) and cloned into a plasmid encoding both a floxed neomycin resistance cassette and a diphtheria toxin negative selection marker ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 10X T4 DNA ligase buffer (5 uL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hours and then 65 °C for 10 minutes.
-
bioRxiv - Molecular Biology 2021Quote: ... DNA guides used for cleavage (Supplementary Table S3) were 5’-phosphorylated using T4 PNK (New England Biolabs) except when cleavage with 5’-OH guides ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA pellets were resuspended in 5 µL dephosphorylation reaction mix (7 mM Tris pH 8, 1x NEB T4 PNK buffer ...
-
bioRxiv - Microbiology 2020Quote: ... 1 μL of 5 μM annealed adaptors and 10 μL of Blunt/TA ligase master mix (NEB) was added to each reaction ...
-
bioRxiv - Microbiology 2021Quote: ... and pvhsp70 5’ UTRs were digested using BlpI and SpeI restriction enzymes (New England Biolabs, Ipswich, MA), and agarose gel-purified fragments were subcloned into the plasmid pD-pfcam-Luc also digested with these enzymes ...
-
bioRxiv - Genetics 2020Quote: ... RNA was then degraded by the addition of 5 U RNase H (New England Biolabs, cat. M0297S) and cDNA from every 2 reactions was purified on a single RNA Clean & Concentrator-5 column and eluted in 36 μl NF H2O.
-
bioRxiv - Cell Biology 2022Quote: ... Hybridized oligonucleotides were phosphorylated with 5 U T4 polynucleotide kinase in T4 Ligation buffer (New England Biolabs) for 30 min at 37 °C ...
-
bioRxiv - Developmental Biology 2020Quote: ... Free adaptor was then removed by addition of 1 μL 10 U/μL 5’-Deadenylase (NEB, M0331S). 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre ...
-
bioRxiv - Cell Biology 2021Quote: ... pStr-KDEL_APV-Dendra2/mCherry/EGFP-ER-5 was constructed by the Gibson assembly (New England BioLabs E2611) of pStr-KDEL_SBP-EGFP-Ecadherin linearized by AscI and XbaI ...
-
bioRxiv - Microbiology 2020Quote: ... This plasmid was then digested with BamHI and NotI and the resulting insert (5’esaAflank_SpecPromoter_kanR_3’esaAflank) was gel extracted (Monarch DNA Gel Extraction Kit, NEB). 100 ng of purified insert was transformed into S ...
-
bioRxiv - Immunology 2021Quote: ... The assembled plasmid pCO2 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... oPool oligos were phosphorylated at their 5’ ends using T4 Polynucleotide Kinase (New England Biolabs, Ipswich, MA). DNA adapters were then annealed to the phosphorylated oPool oligos by incubating extension ...
-
bioRxiv - Cell Biology 2022Quote: ... 5 μg of DNA was digested with HinfI and RsaI restriction enzymes (New England BioLabs, 50U each) at 37 °C overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5’-phosphorylated and dA-tailed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Samples were ligated to adaptor oligos for multiplex sequencing (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H), and isolated with PureYield Plasmid Miniprep System (Promega ...
-
bioRxiv - Genomics 2023Quote: ... Reverse transcription reactions (RT +) were then performed with 5 µg of RNA and random hexamers (NEB, #S1330S), using the Superscript III Reverse Transcriptase (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... pETduet-1::5′-flank_SpecPromoter_kanR_3′-flank and were subsequently digested from the plasmid using BamHI and NotI (NEB). The resultant insert (5′-flank_SpecPromoter_kanR_3′-flank ...
-
bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
-
bioRxiv - Molecular Biology 2022Quote: Approximately 5 μg genomic DNA were digested with 1.5 μl restriction enzymes + 2.5 μl CutSmart buffer (NEB) for 5 hrs at 37°C ...