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Citations for New England Biolabs :
2451 - 2500 of 4087 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared using the NEBNext ARTIC SARS-CoV-2 Library Prep Kit (NEB) and ARTIC V5.3.2 primer sets ...
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bioRxiv - Genomics 2024Quote: ... 100-500 ng of genomic DNA was fragmented using 2 units of Nt.CviPII (NEB # R0626S) in 500 µl of 1X NEBuffer 2 at 37°C for 4 hrs to overnight ...
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bioRxiv - Immunology 2024Quote: ... Sequence libraries were amplified using NEBNext High Fidelity 2× PCR Master Mix (New England Biolabs) and barcoded primers ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µg of DNA was treated with uracil DNA glycosylase (UDG, New England Biolabs, Inc.) for 60 minutes at 37°C with gentle shaking ...
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bioRxiv - Molecular Biology 2024Quote: ... 8.8 µL of the probed RNA were combined with 2 µL 10 mM dNTPs (NEB) and 1 µL 200 ng/µL random nonamer (Random Primer 9 ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM MgCl2) prior to addition of 20 units (2 µl) NruI (New England Biolabs). The restriction digests were carried out at 37 ◦C for 2 hours ...
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bioRxiv - Genomics 2024Quote: ... were prepared using the Q5 Hot Start High-Fidelity 2 × Master Mix (Cat# M0494L, NEB) as follows ...
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bioRxiv - Synthetic Biology 2024Quote: ... was coated with 25 µL/well of 2 µg/µL streptavidin (New England Biolabs; N7021S) diluted in 1x HBS and incubated overnight at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... A molecular weight ladder was prepared using 2 µL of ssRNA ladder (New England Biolabs), 8 µL of RNase-free water (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Following KLD (kinase, ligase, and DpnI) treatment and transformation into NEB 5-alpha F’Iq cells (New England Biolabs), cells were grown as near-lawns on LB-chloramphenicol plates and a miniprep was performed from a resuspension of ~5000 colonies for each library.
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bioRxiv - Genomics 2021Quote: ... the purified products are treated with Klenow fragment (3’ → 5’ exo-) (Cat. No. M0212L; NEB; use 1 uL) and Taq DNA polymerase (Cat ...
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bioRxiv - Biochemistry 2020Quote: ... the top strand (MB733) was 5′ labelled with ϒ[32P]- ATP and T4 polynucleotide kinase (T4 PNK; NEB) for 1 h at 37°C and cleaned up with a G-50 micro column as above ...
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bioRxiv - Developmental Biology 2021Quote: ... the synthesized sgB was injected at 300ng/µL after 5-minute incubation at RT with Cas9 protein (NEB) at 2µM final in 20mM Hepes-NaOH pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 μL (approximately 250 ng) of the extracted DNA was first digested using the restriction enzyme DpnII (NEB), diluted to 100 μL and ligated with T4 ligase (NEB) ...
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bioRxiv - Developmental Biology 2020Quote: ... 37.5 μl of 0.4 mM biotin-14-dATP and 10 μl of 5 U/ μl Klenow (NEB, M0210L) were added and mixed by pipetting ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA was then 5’ end labeled in 24μL PNK wash buffer with 16μL of 1x PNK buffer (NEB) containing 150μCi of gamma P32-ATP ...
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bioRxiv - Neuroscience 2021Quote: NFIB 3’ UTR and 5’ UTR HP forming regions were in vitro transcribed using T7 transcriptase (NEB, E2040) and purified with Trizol extraction (described in RT-qPCR paragraph) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was denatured again and pyrophosphates were removed from the 5’-end by 10 U RppH (M0356S, NEB) in 1× NEB buffer 2 (10 mM Tris-HCl pH 7.9 ...
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bioRxiv - Synthetic Biology 2020Quote: ... was achieved by PCR of the relevant design using primers F_RiboJ_Rem and R_RiboJ_Rem (Supplementary Table 5) and subsequent circularization by standard Golden Gate assembly (New England Biolabs, E1601S) to create the plasmids pVB003 and pVB004 ...
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bioRxiv - Biophysics 2020Quote: ... Fluorescence labelling was done by incubating with 5 nmol SNAP-Surface Alexa Fluor 488 (#S9129S, New England Biolabs) for 1 hour at 4 °C ...
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bioRxiv - Genomics 2020Quote: ... RNA was resuspended in 5 μl of water and the 3′ ends dephosphorylated with PNK (New England BioLabs) in MES buffer (100 mM MES-NaOH ...
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bioRxiv - Plant Biology 2020Quote: ... were amplified from genomic DNA using primers in Supplemental Table 5 and Taq Phusion polymerase (New England BioLabs), cloned in pGEM-t Easy (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... 15 μg of DNA-depleted RNA was incubated with 12.5 units of RNA 5’ pyrophosphohydrolase (RppH) (#M0356, NEB) in a 50 μl reaction for 1 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... the cap-1 structure was added to the 5’ end using the Vaccinia capping enzyme (New England Biolabs) and Vaccinia 2’-O-methyltransferase (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... Chromatin within intact nuclei was then digested for 5 minutes with micrococcal nuclease (NEB #M0247, 500U/1M cells) prior to nuclei lysis by Covaris sonication ...
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bioRxiv - Biochemistry 2021Quote: ... gDNA was quantified by Nanodrop and a fraction of DNA was treated with 5 U RNase H (NEB) for 1h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... Residual template switch adaptor was removed by incubation with 5 U of uracil DNA glycosylase (New England BioLabs) for 40 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: 3’-PUA-DNA was generated by incubation of 1 μM 5’-FAM-labeled AP-DNA (FAM_U_35) with 20 U EndoIII/Nth (NEB) in Buffer B pH 7.0 at 37 °C for 60 m ...
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bioRxiv - Microbiology 2022Quote: ... 2.5µL of 10µM MBTUni-12 primer + 2.5µL of 10µM MBTUni-13 primer (5’-ACGCGTGATCAGTAGAAACAAGG-3’) + 10µL 5x HF Phusion Buffer + 1µL 10mM dNTPs mix (NEB #N0447S) + 0.5µL Phusion Polymerase + 28.5µL of Nuclease-free water (Ambion ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µL of PCR product from the inner primer amplification was cleaned used 0.5 µL Exonuclease I (NEB) and 1 µL rSAP (NEB) ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were incubated on ice for 5 minutes then immediately treated with appropriate concentration of RNAse If(NEB) for 3 mins at 37C at 1200RPM ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
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bioRxiv - Molecular Biology 2020Quote: ... Sample was pelleted at 2500xg for 5 minutes and the pellet was washed with 1x Cutsmart buffer (NEB) and pelleted again ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... and cloned between 300 bp of PCR amplified DNA fragments of the LdBPK_250018100.1 5’ and 3’ UTR using NEBuilder (NEB) inside pUC19 for construct amplification in E ...
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bioRxiv - Immunology 2020Quote: ... and the assembled plasmid pCO1 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 μl 10X ligation buffer, 0.8 μl 100% DMSO, 2.5 μl T4 RNA ligase I – high concentration [NEB] ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA reaction mix was therefore compensated by adding 0.5 μl 5’Ligation Reaction Buffer 10x (NEB-kit) and 1.75 μl nuclease-free H2O.
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Pre-adenylated DNA linker(DNA oligo purchase from IDT was adenylated using 5′ DNA Adenylation Kit (NEB,E2610S)) was ligated to the 3’-end of RNA in a 20 µl reaction (25% PEG8000 ...
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bioRxiv - Molecular Biology 2021Quote: ... by AgeI and EcoRI digestion and ligation of 5’-phosphorylated DNA duplexes using T4 DNA ligase (NEB, #M0202). Target plasmids expressing 0 and 480 CTG repeats in the context of DMPK exons 11-15 (DMPKS and DT480 ...
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bioRxiv - Molecular Biology 2021Quote: ... R: 5’-ccgggaaaaactgaaaaaccattggcacgacaggtttcccgac-3’ from the pKAM555 vector) was inserted at the ClaI site using HiFi assembly (NEB). For the intTEL0 strain creation the pUC19LG plasmid (lacking the PstI(blunted)-BclI fragment of the HARS36 sequence ...
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bioRxiv - Biophysics 2021Quote: ... The phosphorylated 5’-ends were subjected to RNA ligation by 20 units of T4 RNA ligase 1 (NEB) in the presence of 10 mM DNA/RNA rP5_RND oligo (Supplemental Table 3 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 μl 10 mM dNTPs, 0.5 μl 10 μM NEB Universal PCR primer, 0.5 μl 10 μM NEB index primer ...
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bioRxiv - Immunology 2020Quote: ... TAP reaction was performed in a total volume of 25 μL using 5 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: 9N_VRA3 adapter oligonucleotide (Supplementary Table 1) was pre-adenylated with the 5’ DNA Adenylation Kit (New England Biolabs) according to the manufacturer’s protocol at a 5X scale ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.2% Tween-20) and RNA 5’ ends where labelled with [γ-32P]-ATP and T4 PNK (NEB, M0201L) at 37°C for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA adaptor provided by the kit was phosphorylated on its 5’ end using T4 Polynucleotide Kinase (NEB). RNA (1 μg ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA probes (Extended Table 1) at 500 nM were 5’-[32P]-labeled using T4 Polynucleotide Kinase (NEB) and hybridized to the membrane overnight at 37°C in PerfectHyb™ Plus Hybridization Buffer (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA templates were then degraded by incubating reactions with 5 units of RNase H (M0297, New England Biolabs) and 1 μl RNase cocktail enzyme mix (AM2296 ...
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bioRxiv - Biochemistry 2023Quote: Pre-crRNA was 5’-radiolabeled with [γ−32P] ATP (Perkin-Elmer) using T4 polynucleotide kinase (New England Biolabs). For some measurements ...
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bioRxiv - Molecular Biology 2023Quote: ... For ligation, samples were washed in CutSmart buffer, preincubated (5 min, RT) in 1x T4 ligase buffer (NEB), and incubated (18 h ...