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Citations for New England Biolabs :
201 - 250 of 453 citations for Recombinant Human Metadherin GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The 3xFLAG- and HA-tagged ZNRF3 expression vectors were generated with the Q5 Hot Start High-Fidelity DNA Polymerase Kit (New England Biolabs) by replacing the FLAG-tag ...
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bioRxiv - Cell Biology 2023Quote: ... To remove RNA secondary structure and anneal the mRNA capture primer 1 μL of tagged random hexamer (100 μM) and 0.5 μL of 10 mM dNTPs (dNTP solution set NEB - N0446S) were added to the sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids and siRNAs Bacterial expression constructs for His6-tagged p97 fragments were generated by Q5 Site-Directed mutagenesis (New England Biolabs) of pET15b-His6-p97 plasmid ...
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bioRxiv - Molecular Biology 2023Quote: Expression vectors encoding His6/SUMO-tagged SAHS proteins were obtained from Twist Bioscience and transformed into LEMO21(DE3) competent cells (New England Biolabs) according to the provider’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... myc-tagged AP3B1 immunoprecipitated and immobilised on Protein G-sepharose beads was incubated with CK2 (250 units; New England Biolabs) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... brucei BFs CN PIP5Pase exclusively expressing V5-tagged PIP5Pase WT or mutant D360A/N362A for 24 h using the magnetic mRNA isolation kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the SNAP-tagged fragment was assembled into a ApaI digested MBO2-HA plasmid using NEBuilder (New England Biolabs, Ipswich, MA). The final N-SNAP-MBO2-HA construct encodes an MBO2 polypeptide with a SNAP tag at its N-terminus and a 2-HA tag located between amino acids 885 and 886 of the original MBO2 sequence (Supplemental Figure S1).
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bioRxiv - Molecular Biology 2024Quote: ... 5 µM purified SNAP-tagged proteins were reacted with 10 µM biotin-conjugated benzylguanine (BG-bio) (S9110S; New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were grown on glass coverslips and pre-existing SNAP-tagged histones were fluorescently labelled with 2 μM of the red-fluorescent reagent SNAP-cell TMR star (New England Biolabs) during a 30 min-pulse step followed by 30 min wash in fresh medium ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were grown on glass coverslips and SNAP-tagged histones were fluorescently labelled with 2 μM of the red-fluorescent reagent SNAP-cell TMR star (New England Biolabs) during a 30 min-pulse step ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Immunology 2021Quote: ... The recombinant plasmids (LentiCRISPRv2.gRNA10, LentiCRISPRv2.gRNA11) were then transformed into competent Escherichia coli (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins were expressed in Escherichia coli T7 Express lysY/Iq cells (New England Biolabs). For all proteins except PP1γ ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant plasmid was generated by a NEBuilder® HiFi DNA Assembly (New England Biolabs) reaction ...
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bioRxiv - Cell Biology 2020Quote: Recombinant MBP fusions to Nud1 or Nud17A were expressed from pMAL-c4X (New England Biolabs). Induction in Rosetta 2 cells (Novagen ...
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bioRxiv - Cancer Biology 2021Quote: Trastuzumab deglycosylation was achieved by adding 1.1 units of recombinant PNGaseF enzyme (New England BioLabs) per 1 μg of antibody ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant SARS-CoV-2 Omicron RBD was digested with EndoH over night (New England Biolabs). Recombinant hACE2 protein was digested using EndoH (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... Ribonucleoprotein (RNP) complexes were formed from sgRNA and recombinant Cas9 2NLS protein (New England Biolabs) mixed at a sgRNA to Cas9 ratio of 4.5 ...
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bioRxiv - Biochemistry 2022Quote: ... To complete the recombinant assembly reaction NEBuilder® HiFi DNA Assembly kit (New England Biolabs) was used with the combination of three different inserts produced by PCR ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction was initiated by addition of 10 U (pmol/min) of recombinant CK1 (NEB), CDK1-cyclin B1 (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reaction was started by adding 5000 units of recombinant Casein Kinase II (NEB, P6010S) and left to incubate for 20 min at 30°C with gentle agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 mM EDTA pH 8.0) and 1.6 U of recombinant proteinase K (New England Biolabs) was added ...
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bioRxiv - Cell Biology 2024Quote: ... Recombinant MBP-PP1α87BWT or MBP-PP1α87BT286D were purified with amylose magnetic beads (New England Biolabs) and eluted with 10 mM Maltose ...
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bioRxiv - Microbiology 2021Quote: ... 40 U RNase Inhibitor Human Placenta (NEB), 50 pmol Oligo d(T)23 (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... TIF3 alleles with the native promoter and terminator encoding C-terminal hexahistidine tagged-eIF4B or variants were Gibson-cloned (NEB, USA) into the BamHI site of single-copy vector pHLUM (21) ...
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bioRxiv - Biochemistry 2021Quote: PCR of the tagged DNA was performed in 25 μl reactions in 200 μl thin-walled PCR tubes containing 1X Q5® Buffer (NEB), 1X Q5® GC Enhancer (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... was performed using 10ng of His tagged PTP-PEST (WT) plasmid as template and followed by Dpn1 (New England Biolabs, UK) digestion for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated as follows: HA-tagged and untagged ORFs with pHCMV homologous overhanging sequence were either PCR amplified using Q5 polymerase (NEB, M0491S) or synthesized (IDT ...
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bioRxiv - Cell Biology 2021Quote: ... were subcloned into phCMV3 to express C-terminal FLAG-tagged CatSper subunits (phCMV3-CatSperd or z-Flag) using NEBuilder® HiFi DNA Assembly Kit (NEB). A stop codon was placed at the upstream of HA-encoding sequences of phCMV3 vector for FLAG-tagged CatSper subunit cloning.
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bioRxiv - Molecular Biology 2020Quote: ... The domain deletion mutants of pCS2+ FLAG-tagged SRSF1 construct were generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The primers sequences are given in Supplementary Table S1.
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bioRxiv - Genomics 2021Quote: ... and introduced between XhoI and BamHI sites in c-Flag pcDNA3 (addgene #20011) to generate Flag-tagged hPEX5 using NEBuilder® HiFi DNA assembly Master mix (New England Biolabs). Site-directed mutagenesis for amino acid substitution was performed using the Q5® Site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... Short hairpin resistant chimeric mutants of Flag-tagged GLUT3 alleles were generated using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: All purified SNAP-tagged proteins were labeled with 10-fold molar excess of SNAP-Surface dyes (New England Biolabs, Ipswich, MA) in labeling buffer (1× PBS ...
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bioRxiv - Microbiology 2023Quote: ... 1990) tagged with Illumina adapters in PCR reactions using a Q5® Hot Start High-Fidelity DNA Polymerase (New England Biolabs). The 25 amplification cycles were as follows ...
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bioRxiv - Molecular Biology 2024Quote: Plasmids (pBluescript KS+, pBS hereon) for HiBit-tagged POI mRNA were generated by Gibson Assembly (Gibson Assembly cloning kit, NEB, E5510S) or restriction digest cloning ...
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bioRxiv - Biophysics 2024Quote: ... An in-house vector with H1.8 tagged with N-terminal eGFP tag and 6x Histidine-tag was transformed in T7 Express cells (enhanced BL21 derivative, NEB C2566I) and grown to an OD of ≈ 0.7 at 37 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The biotinylated and 6xHis-tagged screens were immunoprecipitated overnight with streptavidin or Ni-NTA magnetic beads (New England Biolabs, Ipswich, MA), respectively ...
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bioRxiv - Microbiology 2021Quote: ... the plasmid digestion was supplemented with 1.5 units of recombinant shrimp alkaline phosphatase (rSAP; NEB #M0371S). Digested insert and vector were purified and ligated together at room temperature for 30 min using T4 DNA ligase (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... after which 2 µL of Recombinant PNGaseF (Glycerol-free) (New England Biolabs, Ipswich, MA; catalog # P0709L) was added to each sample ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed in Escherichia coli T7 Express lysY/Iq cells (New England Biolabs). Except for GST-PP1γ ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant SYNJ2BP (0.5 µg) was dephosphorylated using 1 µl of calf intestinal alkaline phosphatase (CIP) (NEB) in 1x CutSmart Buffer in a total volume of 10 µl ...
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bioRxiv - Molecular Biology 2023Quote: ... the enzymatic activity of the commerically availablale recombinant vaccinia virus methyltransferase mRNA 2’O-methyltransferase (NEB), abbreviated hereagter as VMTR1 ...
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bioRxiv - Neuroscience 2024Quote: ... and used to form ribonucleoprotein (RNP) complexes when combined with recombinant Cas9 protein (New England Biolabs). Ribonucleoprotein complexes were formed using the method of Kouranova49 ...
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bioRxiv - Bioengineering 2024Quote: Isothermal Calorimetry: Recombinant hSTING was synthesized in Escherichia coli (New England Biolabs, T7 SHuffle Express line) and purified by affinity chromatography ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant protein of AP2 domains fused with MBP (MBP::PbSIP2) was purified using amylose resin (NEB) and recovered in 10 mM maltose solution.
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bioRxiv - Microbiology 2023Quote: ... MARCH2 chimeras containing the TM domains from either human MARCH4 or human transferrin receptor (TR) were generated using the NEBuilder HiFi DNA assembly kit (New England Biolabs) and the primers listed in Supplemental Table S2 ...
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bioRxiv - Biochemistry 2023Quote: Human HA-DHFR was subcloned from human cDNA to pcDNA4/TO by PCR using Q5 High Fidelity 2X mastermix (NEB). Human FPGS-FLAG and GGH-FLAG were subcloned from cDNA clones MHS6278-202755815 (Horizon Discovery ...
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bioRxiv - Microbiology 2023Quote: Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
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bioRxiv - Biophysics 2020Quote: ... from human origin were all purchased from NEB. The H2A/H2B and H3.1/H4 were mixed in 2(H2A/H2B)2:1(H3/H4)4 molar ratio to form octamers.
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bioRxiv - Cancer Biology 2024Quote: ... or rabbit anti human HMGA2 (New England Biolabs), ...