Labshake search
Citations for New England Biolabs :
201 - 250 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... using NEB HIFI assembly kit (NEB, #5520s). The heavy chains were cloned into human IgG1 or customer-designed human IgG4-His vector ...
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bioRxiv - Neuroscience 2023Quote: ... Q5® Site-Directed Mutagenesis Kit (NEB) was used with mutagenesis primers (Fwd ...
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bioRxiv - Microbiology 2023Quote: ... Monarch® PCR & DNA Cleanup Kit (NEB), or Gene Jet gel extraction kit (Thermo Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... The Q5 Site-directed mutagenesis kit (NEB) was used to generate CMV::opti2-ACD6-1 and CMV::opti2-ACD6L557F ...
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bioRxiv - Bioengineering 2023Quote: ... and Monarch RNA Cleanup Kit (NEB T2040L) for in vitro transcription and purification were purchased from New England Biolabs ...
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bioRxiv - Genetics 2023Quote: ... The Monarch Total RNA Miniprep kit (NEB) was then used for gDNA elimination and final purification of RNA ...
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bioRxiv - Genetics 2023Quote: ... or the Monarch HMW DNA kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... or the NEB HiFi Assembly kit (NEB). All primers are listed in table S3 ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... and Monarch® RNA purification kit (NEB) was used to purify the final mRNA according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... NEB stranded RNA library prep kit (NEB) and NEB Ultra II RNA library prep kit (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... and Monarch PCR Cleanup Kit (NEB, T1030). PCR primers used ...
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bioRxiv - Biochemistry 2023Quote: ... The Q5 Site-Directed Mutagenesis Kit (NEB) was used to generate pcDNA3.1+/C-(K)-DYK-IRF5-T265A ...
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bioRxiv - Synthetic Biology 2023Quote: ... using NEBridge Golden Gate Assembly kit (NEB), with custom designed primers ...
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bioRxiv - Developmental Biology 2024Quote: ... The NEBuilder HiFi DNA Assembly Kit (NEB) was used for the construction of chimeric Pk1 and Pk2 mutants ...
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bioRxiv - Cell Biology 2024Quote: ... and Monarch RNA Clean Up Kit (NEB). Triplicated samples were pooled prior to sequencing.
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bioRxiv - Developmental Biology 2024Quote: ... Monarch PCR purification kit (Biolabs, New England) used to purify the resulting amplified cDNA constructs ...
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bioRxiv - Cell Biology 2024Quote: ... the Q5 Site-Directed Mutagenesis Kit (NEB) was used to delete the miniTurbo sequence from the above pLVX-KRT5-miniTurbo-FLAG-IRES-Puro and pLVX-KRT6A-miniTurbo-FLAG-IRES-Puro constructs ...
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bioRxiv - Microbiology 2024Quote: ... or a Monarch gDNA extraction kit (NEB). Bacterial genomic sequencing was performed by either MinION Mk1C nanopore sequencing or PacBio sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... Q5 Site-Directed Mutagenesis Kit (E0554S, NEB), Recombinant protein G sepharose beads (101242 ...
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bioRxiv - Molecular Biology 2024Quote: ... using NEBuilder HiFi DNA assembly kit (NEB). pBAD-His6-Ocr was obtained by cloning 0.3 gene from T7 phage into pBAD-HisB43 vector using Gibson Assembly Master Mix (NEB) ...
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bioRxiv - Systems Biology 2024Quote: ... HiScribe® SP6 RNA Synthesis Kit (NEB) was used to in-vitro transcribe a library of mRNA reporters from linearized plasmids ...
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bioRxiv - Synthetic Biology 2024Quote: ... or Monarch® Plasmid Miniprep Kit (NEB). When purifying plasmid DNA from C ...
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bioRxiv - Genomics 2024Quote: Strand cDNA Synthesis Kit (New England Biolabs) according to manufacturers recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: DNA libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit kit by New England Biolabs (NEB) and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Genomics 2020Quote: NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext rRNA Depletion Kit (NEB, Ipswich, MA) was used to generate RNA-seq libraries from purified RNA following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Libraries were constructed using NEBNext Ultra RNA Library Prep for Illumina kits (New England Biolabs kits E7490 and E7530), as per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... a ChIP-seq library was made using the NEBNext Ultra II DNA Library Prep Kit for Illumina kit (NEB).
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bioRxiv - Cell Biology 2023Quote: The novel entry modules created for this study were cloned by insertion of PCR-amplified sequences in pJet1.3 or pMiniT 2.0 following manufacturer’s instructions (CloneJET PCR Cloning Kit, Thermo Scientific; NEB® PCR Cloning Kit, NEB). BsaI recognition sites followed by module-specific overhangs were added to each primer used to amplify entry sequences ...
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bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
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bioRxiv - Developmental Biology 2023Quote: ... libraries were quantified by qPCR using a library quantification kit (NEBNext® Library Quantification Kit, E7630, New England Biolabs). Illumina sequencing was performed on a HiSeq2500 instrument for 100 single end read cycles (Genome Sequencing Core ...
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bioRxiv - Genomics 2023Quote: ... Final immunoprecipitated DNA samples from the kit were then purified using a Monarch PCR and DNA cleanup kit (5μg) (NEB), with a final elution volume of 50μL in TE buffer ...
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bioRxiv - Cell Biology 2024Quote: ... All mGreenLantern-tagged constructs consist of ORFs inserted into the multiple cloning site of pcDNA3.1-mGreenLantern using a Gibson cloning kit (NEBuilder HiFi DNA Assembly Cloning Kit, New England Biolabs). Intersectin(SH35 ...
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bioRxiv - Developmental Biology 2021Quote: ... the RNA samples isolated by PureLink Mini kit were subsequently purified by Magnetic mRNA Isolation kit (New England Biolabs, USA). mRNA concentration was estimated by Qubit RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... amplicons were gel purified with the InnuPREP Gel Extraction Kit (Analytik Jena) or the Monarch DNA Gel Extraction Kit (NEB), and submitted for Sanger sequencing (Eurofins Genomics) ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted using the Qiagen RNeasy Micro kit and libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs). All libraries were sequenced on an Illumina HiSeq 2500 with 100 bp single-end reads.
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bioRxiv - Molecular Biology 2021Quote: ... Adaptor and index primers were added to the sheared DNA fragments using a next-generation sequencing library preparation kit (NEBNext Ultra DNA Library Prep Kit for Illumina; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted using illustra RNAspin Mini isolation kit (Cytiva) or Monarch Total RNA Miniprep Kit (New England Biolabs) subsequent to spheroblast construction using 10 U of Zymolyase (ZYMO Research ...
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bioRxiv - Developmental Biology 2019Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II library prep kit with the NEB Unique Dual Index primer set kit (New England Biolabs). Samples were sequenced at the Northwestern University NuSeq Core Facility on an Illumina HiSeq 4000 on single-end 50 bp mode ...
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bioRxiv - Microbiology 2019Quote: ... using Agilent High Sensitivity DNA kit in a Bioanalyzer 2100 instrument then quantitated using qPCR based NEBNext Library Quant Kit for Illumina (New England Biolabs) with a Piko-Real Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNA libraries were generated using NEBNext rRNA Depletion kit combined with NEBNext Ultra II Direction RNA Library Prep kit for Illumina (New England Biolabs). We used 100 ng of starting RNA material for input and pre-sorted synaptosomes and 1-5 ng for vGLUT1+ sorted synaptosomes which corresponds to ~100 Mio synaptosomes collected per P3 fractions ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples with RIN value higher than 8 were processed using the NEBNext poly(A) mRNA Magnetic Isolation kit and the NEBNext Ultra II RNA Library Prep kit for Illumina (New England Biolabs), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR/reaction cleanup and gel extraction were conducted with the Monarch PCR and DNA cleanup kit and Monarch DNA gel extraction kits (NEB). E ...
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bioRxiv - Microbiology 2021Quote: ... cholerae and phages was extracted using commercially available kits (Qiagen DNeasy Blood and Tissue or Monarch genomic DNA purification kit (New England BioLabs). For preparation of phage DNA ...
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bioRxiv - Microbiology 2022Quote: ... After enrichment of mRNA using the Universal Ribodepletion Kit cDNA preparation was performed with the Next Ultra Directional Library Preparation Kit for Illumina (NEB). The cDNA was sequenced on HiSeq 3000 (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... 60 ng of total RNA was used for library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext rRNA Depletion Kit (New England Biolabs). The libraries were paired-end sequenced (2 × 75 bp ...
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All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cellsbioRxiv - Neuroscience 2021Quote: ... and RNA was consecutively isolated using a column based RNA isolation kit according to the manufacturer’s instructions (Monarch® Total RNA Miniprep Kit; #T2010S New England Biolabs). Strand specific cDNA library preparation from polyA enriched RNA (150 bp mean read length ...
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bioRxiv - Immunology 2019Quote: ... The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description.
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bioRxiv - Systems Biology 2021Quote: Bulk RNA-seq libraries were prepared from 1000 ng of RNA using the NEBNext Ultra RNA Library Prep kit for Illumina or the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enrichment of mRNA was done using the Universal Ribodepletion Kit followed by Next Ultra Directional Library Preparation Kit for Illumina (NEB). The cDNA was sequenced on HiSeq™ 2500 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Libraries were constructed from 1 µg of total RNA using Illumina’s TruSeq stranded mRNA Library Preparation Kit or from 500 ng total RNA using NEBNext Ultra II RNA -polyA+ (mRNA) Library Preparation Kit (New England Biolabs) following the manufacturer’s description.