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Citations for New England Biolabs :
2351 - 2400 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... sequencing using NEBNext UltraII DNA library Prep Kit for Illumina (New England BioLabs, Inc) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: Libraries were prepared using either a NEBNext DNA Ultra II kit (New England BioLabs) or an Illumina TruSeq DNA Sample Prep Kit (Table S5) ...
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bioRxiv - Microbiology 2019Quote: ... Constructs were then prepared using the Q5 site-directed mutagenesis kit (NEB cat #E0554) or by Gibson assembly (using the NEBuilder HiFi assembly mastermix ...
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bioRxiv - Molecular Biology 2019Quote: Sequencing library preparation was performed with NEBNext Ultra DNA library prep kit (NEB #E7370S) and NEBNext Multiplex Oligos (NEB #E7335S ...
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bioRxiv - Synthetic Biology 2019Quote: ... cloning was performed with the NEBuilder Hi Fi DNA Assembly kit (New England Biolabs). PCR reactions were performed with the Phusion High-Fidelity PCR Master Mix with HF buffer (New England Biolabs) ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA was amplified by RT-PCR (Phusion High Fidelity Polymerase kit, New England Biolabs) using primers listed in Methods S3.
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bioRxiv - Developmental Biology 2019Quote: ... Libraries were then quantified by qPCR using NEBNExt Library Quant Kit for Illumina (NEB) on QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was purified by using a Monarch® PCR&DNA cleanup kit (NEB #T1030) before analyzing the DNA by capillary electrophoresis (30) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The cleaned DNA was then used in the KLD Enzyme Kit (New England Biolabs) with 1 μL KLD Enzyme Mix ...
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bioRxiv - Immunology 2020Quote: ... Depletion of ribosomal RNA was performed using Next rRNA Depletion kit (New England BioLabs). RNA quality was confirmed by bioanalyser (Agilent 2100 Bioanalyzer G2938B) ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was reverse transcribed into cDNA using a LunaScript RT SuperMix Kit (NEB, E3010L) by following the manufacturer’s protocol ...
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bioRxiv - Zoology 2021Quote: ... the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs® Inc.) protocol was followed ...
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bioRxiv - Molecular Biology 2020Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... we cloned pcDNA3.1-SARS2-SpikeD614G using the Q5 site-directed mutagenesis kit (NEB E0554S) and the following primers ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA libraries were generated using the NEBNext Ultra II DNA Library Preparation kit (NEB). Samples were sequenced by 40 bp paired-end sequencing using a NextSeq 500 (Illumina).
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bioRxiv - Molecular Biology 2020Quote: ... and NGS libraries were quantified by qPCR using the NEBNext Library Quant Kit (NEB). Illumina sequencing was performed with a NextSeq mid-output kit with 150-cycle single-end reads and automated adaptor trimming and demultiplexing (Illumina).
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bioRxiv - Synthetic Biology 2021Quote: ... The PCR products were later purified with the Monarch Purification Kit (NewEngland Biolabs, #T1030S) prior to deep sequencing analysis ...
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bioRxiv - Biochemistry 2020Quote: ... IVT was performed with HiScribe T7 High Yield RNA synthesis kit (New England BioLabs) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified (Monarch® PCR & DNA Cleanup Kit 5µg; New England BioLabs) and sequenced from both sides with Eurofins Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... After depleting ribosomal RNA (rRNA) using NEBNext rRNA Depletion Kit (New England Biolabs #E6350L), RNA was reversely transcribed using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs #E7765S ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were assembled using Gibson assembly (NEBuilder HiFi DNA Assembly Cloning Kit, NEB E5520S). Fragments were either PCR amplified with a least 15 bp overlap at each end (Phusion Hot Start Flex NEB M0535L ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR was performed on plasmid DNA with Phusion High Fidelity PCR Kit (NEB). PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB ...
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bioRxiv - Molecular Biology 2019Quote: All MKRN1 mutant plasmids were generated with the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2019Quote: ... All cloning was done using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB #E5520). PCRs were done using Q5® High-Fidelity DNA Polymerase (NEB #M0491).
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bioRxiv - Microbiology 2019Quote: ... Libraries were constructed using the NEBNext Ultra DNA Sample Prep Master Mix Kit (NEB), with minor modifications and a custom automated protocol on a Biomek FX (Beckman) ...
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bioRxiv - Genetics 2019Quote: The homologous repair constructs were created using the NEBuilder HiFi DNA Kit (NEB, E5520S) and contained 1,000 bases upstream of the first guide RNA target Cas9 site ...
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bioRxiv - Biophysics 2019Quote: ... Mutant constructs were generated using a site-directed mutagenesis kit (Q5, New England Biolabs) using primers described in the supplement ...
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bioRxiv - Biochemistry 2019Quote: We introduced mutations in phAPOBEC3B-HA using the Q5 Site-Directed Mutagenesis Kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We performed cDNA synthesis following standard protocols with the ProtoScript cDNA synthesis kit (NEB) using 1 µg of RNA for each sample ...
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bioRxiv - Genetics 2020Quote: ... cDNA was generated using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs). RT-qPCRs were performed on the Light Cycler 480II (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... The NEBNext Poly(A) mRNA magnetic isolation kit (New England Biolabs, Ipswich MA, E7490) was used to purify mRNA from the total RNA ...
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bioRxiv - Microbiology 2019Quote: ... For eight samples the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) and NextSeq (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... and mutations were made using the Q5 Site-directed mutagenesis kit (New England Biolabs). The BacMam vector was a kind gift from Professor Eric Gouaux (Vollum Institute).42 Briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... and used for cDNA library generation with the NEBNext Ultra kit (New England BioLabs), without enrichment ...
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bioRxiv - Cell Biology 2020Quote: ... using the Q5 quick mutagenesis kit (purchased from New England BioLabs, catalog number E0554s) and following the protocol provided by the manufacturer ...
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bioRxiv - Synthetic Biology 2021Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB cat# E7760L). 500 ng RNA was used at input ...
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bioRxiv - Genomics 2021Quote: ➢ Distinct from currently used length 8 barcodes from common library kits (Illumina TruSeq, NEB)
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was generated using Protoscript II First Strand cDNA Synthesis kit (New England Biolabs). cDNAs were diluted 1:20 and then qPCR was carried out in triplicate using the Luna Universal qPCR Master Mix (New England Biolabs) ...
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bioRxiv - Biochemistry 2021Quote: ... Resulting gRNA transcription was performed with HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocol and gRNA was purified and verified for concentration and RNA integrity ...
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bioRxiv - Immunology 2021Quote: ... Libraries were then constructed with the NEB Next Ultra II FS kit (E6177S, NEB) using the prime-seq specifications ...
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bioRxiv - Microbiology 2022Quote: ... Then Trizol was added before RNA was extracted using Monarch RNA Cleanup Kit (NEB), skipping the gDNA cleaning column steps ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA libraries were prepared using the NEBNext Ultra II DNA Library Prep kit (NEB) with the NEBNext Poly(A ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted using 5x107 cells and Monarch Total RNA Miniprep Kit (NEB) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... purified from 1.5% agarose and quantified with NEBNext Library Quant Kit for Illumina (NEB). Sequencing was performed on Illumina HiSeqV4 SR50.
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bioRxiv - Synthetic Biology 2021Quote: Total RNA was extracted from cell-free expression reactions with a kit (NEB #T2010), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product was purified using the Monarch PCR & DNA clean-up kit (NEB). All library preparation and sequencing was carried out at the Vanderbilt Technologies for Advanced Genomics (VANTAGE ...
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bioRxiv - Microbiology 2021Quote: ... Mutagenesis was performed in two steps using Q5 site-directed mutagenesis kit (NEB, USA) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: The pIGLR-2∷mCherry construct was generated with a Gibson assembly cloning kit (NEB) with the following four fragments ...
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bioRxiv - Genomics 2021Quote: ... Single-nucleotide point mutations were generated using a Q5 site-directed mutagenesis kit (NEB) and confirmed by Sanger sequencing ...
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bioRxiv - Biophysics 2020Quote: ... Mutagenesis of STEAP construct was performed using the Q5 Site-Directed Mutagenesis Kit (NEB). The primers used in this study are listed in Table S1 ...