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Citations for New England Biolabs :
2351 - 2400 of 6227 citations for 1 1' 3' 1'' Terphenyl 4 4'' dimethanamine 5' 4 aminomethyl phenyl 9CI since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... 1 mM DTT) with or without 200 µM ATP (New England Biolabs) for 30 minutes at room temperature with end-to-end rotation ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg RNA was reverse transcribed using LunaScript RT SuperMix Kit (NEB). qPCR was performed utilising appropriate primers ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA) and passed through an amylose resin column (NEB#E8021L). Columns were washed prior to recombinant protein elution with 10 mM maltose in column buffer.
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bioRxiv - Genomics 2024Quote: ... gDNA was first incubated with 1 µL ENDOIV (NEB, 10 U/µL) in 1x ThermoPol Buffer (NEB ...
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bioRxiv - Genomics 2024Quote: ... and 30 U of T4 RNA Ligase 1 (30 U/mL, NEB). The sample was incubated at 37°C for 1 h.
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bioRxiv - Biochemistry 2024Quote: The reactions were stopped by adding 1 µL of Proteinase K (NEB) to the sample and incubation at 37°C for 15 min ...
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bioRxiv - Biochemistry 2024Quote: The reactions were stopped by adding 1 µL of Proteinase K (NEB) to 7 µL of the sample and incubation at room temperature for 5 min ...
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bioRxiv - Genomics 2024Quote: ... with 1 μL T4 DNA Ligase Reaction Buffer (NEB, catalog no. B0202S), 0.125 μL T4 DNA Ligase (NEB ...
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bioRxiv - Cell Biology 2024Quote: PCR mixes containing 1 U of Q5 polymerase (M0491S, New England Biolabs), 1x reaction buffer ...
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bioRxiv - Biochemistry 2024Quote: A 1 % agarose gel containing 1X Sybr Safe staining (New England Biolabs) was cast ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µg of the PX330 Cas9 plasmid was digested with BbsI (NEB) following manufacturer’s protocol and purified by agarose gel ...
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bioRxiv - Genetics 2024Quote: ... followed by treatment with T7 endonuclease 1 (M0302S, NEB, Ipswich, MA, USA). For hMPZL2WT mice ...
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bioRxiv - Microbiology 2020Quote: ... and a “bottom” strand with sequence 5’-AAAAC-(30bp spacer reverse complement)-3’ were phosphorylated with PNK (NEB, M0201S) in a 50 μL reaction volume (1.5 μL 100 uM top oligo ...
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bioRxiv - Molecular Biology 2022Quote: ... The 5’ end of the first block and the 3’ end of the last block contained SapI (NEB, R0569) recognition sites instead ...
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bioRxiv - Molecular Biology 2020Quote: ... Selectively captured polyadenylated RNAs (1μg) were ligated directly to an DNA/RNA hybrid adapter (5’-CTACAC GACGCTrCrUrUrCrCrGrArUrCrUrNrNrN-3’) using T4 RNA ligase (NEB) at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Construct included 5’ and 3’ complementary overhangs for ligation into pPICZαA using NEBuilder HiFi DNA Assembly (New England Biolabs) to form pPICZαA_GH43_34 ...
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bioRxiv - Plant Biology 2021Quote: ... samples were incubated for 5 min on ice and 3 μL of 10X G5 buffer (New England BioLabs, UK) were added to the samples together with 1.5 μL of 25X concentrated protease inhibitor cocktail (Roche ...
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bioRxiv - Genomics 2022Quote: ... then A-tailed by incubating for 1 h at 37°C with 200 μM dATP and 0.2 U/μL Klenow fragment (3’-5’ exo-) in NEBuffer 2 (NEB), then Illumina PE adapter was added by incubating overnight at 20°C with 15 μM PE adapter and 2000 U T4 DNA ligase in ligase buffer ...
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bioRxiv - Genomics 2023Quote: The standard HiDEF-seq v2 protocol was followed with the exception of replacing Klenow fragment 3’→5’ exo-polymerase with one of the following: 9.6 U Bst large fragment (NEB), 9.6 U Bst 2.0 (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the second linker SI183 5’-/Phos/NNAGATCGGAAGAGCGTCGTGTAGGGAAAGAG/ddC/-3’ was preadenylated by Mth RNA Ligase (New England Biolabs) as described previously8 ...
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bioRxiv - Microbiology 2023Quote: ... the 5’-PPP RNA in the total RNA was specifically capped with 3’-Desthiobiotin-GTP (New England BioLabs, N0761) by the Vaccinia Capping System (New England BioLabs ...
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bioRxiv - Genomics 2023Quote: ... 5 µg of genomic DNA was dephosphorylated by 3 µL of Quick Calf Intestinal Phosphatase (CIP, New England Biolabs) in a total volume of 30 µL for 10 min at 37°C ...
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bioRxiv - Genomics 2024Quote: ... Two cycles of whole genome amplification were performed using 50 U of Klenow Fragment (3’→5’ exo-) (NEB, M0212M), dNTP solution mix (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... 2020) and 5’Ncol1::mScarlet::3’Ncol119 plasmids using Gibson assembly according to the manufacturer recommendations (NEB, CAT#E2611). Sequences of Gibson primers used were:
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bioRxiv - Bioengineering 2024Quote: ... Genomic DNA was dephosphorylated before Cas9 cleavage and dA-tailing (5 μg gDNA, 3 μL Quick CIP (NEB, M0525S), 3 μL 10X rCutSmart buffer (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) (NEB: E7335L). Samples with total RNA concentration of >10 ng/µl (0.5 µg total amount ...
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bioRxiv - Genetics 2021Quote: ... then a final 1 min extension (72 °C)—using Phusion Flash polymerase (NEB) from cDNA clones using primers containing an EagI restriction site (forward primers ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1 μL of 10 mM dNTPs (New England Biolabs, Ipswich, MA, USA) to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) by NEB following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 µl of T4 or T7 DNA Ligase (New England Biolabs, Ipswich, USA), 1 µl of BsaI-HFV2 (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL Bst 2.0 WarmStart® DNA polymerase (0.32 u; Biolabs, Ipswich, MA), 1 μL Hydroxy Naphthol Blue (HNB ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μL of 1× PNK mix (2 μL of 10× PNK buffer (NEB), 2 μL ATP (SCP801 ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was phosphorylated for 1 h at 37oC with T4 Polynucleotide kinase (NEB or made in-house by the Molecular Biology Service ...
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bioRxiv - Cell Biology 2022Quote: ... 1/10 T7 RNA polymerase mix (HighScribe T7 High Yield RNA synthesis NEB)) at 37°C ...
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bioRxiv - Genomics 2022Quote: ... 1 or 2 μl of 1U/μl USER® II enzyme (M5505S, NEB) and nuclease-free water to made up to 10 μl ...
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bioRxiv - Microbiology 2021Quote: ... 10 μM dNTPs (0.5 μl per reaction) in 1× Klenow reaction buffer (NEB). Next ...
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bioRxiv - Immunology 2021Quote: ... The parental caspase-1 plasmid (methylated) was digested using DpnI (NEB; Cat,# R0176S). Plasmids were transformed in E.coli DH5α competent cells (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The parental plasmid molecules were inactivated by DpnI treatment (1 h, NEB, USA) and the crude reaction mixture was transformed to electrocompetent E ...
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bioRxiv - Genomics 2021Quote: ... 1 µL of 10 U/µL polynucleotide T4 kinase and PNK buffer (NEB) in 20 µL ...
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bioRxiv - Developmental Biology 2021Quote: Plasmid pJM1 (Pceh-23_L::mKate2::rgl-1) was generated using Gibson Assembly (NEB) of PCR products ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by the addition of 1 µl RNase H (5U final, NEB M0297L) and incubation for 20 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... in 1 x Cutsmart buffer (New England Biolabs GmbH, Frankfurt am Main, Germany) for 60 min and then the enzymes heat inactivated at 80°C for 20 min according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ~1 μg of tsetse saliva was treated with PNGase F (New England Biolabs). Samples were run on a 12.5% polyacrylamide gel ...
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bioRxiv - Bioengineering 2020Quote: ... a 10x Cas12-gRNA complex was prepared using 1 μM of LbCas12a (NEB) and 0.5 μM of Cy5-labeled gRNA ...
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bioRxiv - Biochemistry 2020Quote: ... The template plasmid molecules were inactivated by DpnI treatment (1 h, NEB, USA) and directly transformed to electrocompetent E ...
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bioRxiv - Systems Biology 2020Quote: ... MnCl2 to a concentration of 1 mM and NEBuffer for Protein MetalloPhosphatases (NEB) diluted to a 1X concentration were added to both tubes ...
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bioRxiv - Cell Biology 2021Quote: ... containing 1% Triton X-100 and 30 Units of T4 DNA ligase (NEB), followed by ligation reaction at 16°C for 4.5 h and then at room temperature for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF) and proteins were purified by amylose beads (New England Biolabs) and eluted in wash buffer (20mM Tris pH:7.6 ...
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bioRxiv - Systems Biology 2021Quote: ... 25 μL of amplicons were digested with 1 μL of DpnI (NEB R0176S). 100-200 ng of purified digestion was ligated by T4 DNA ligase (Promega M1801) ...
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bioRxiv - Genomics 2021Quote: ... for 1 hour at 37°C in the presence of CutSmart buffer (NEB). To achieve combined DNA purification and selection of high molecular weight fragments ...