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Citations for New England Biolabs :
2301 - 2350 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified (Monarch® PCR & DNA Cleanup Kit 5µg; New England BioLabs) and sequenced from both sides with Eurofins Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... After depleting ribosomal RNA (rRNA) using NEBNext rRNA Depletion Kit (New England Biolabs #E6350L), RNA was reversely transcribed using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs #E7765S ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were assembled using Gibson assembly (NEBuilder HiFi DNA Assembly Cloning Kit, NEB E5520S). Fragments were either PCR amplified with a least 15 bp overlap at each end (Phusion Hot Start Flex NEB M0535L ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR was performed on plasmid DNA with Phusion High Fidelity PCR Kit (NEB). PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB ...
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bioRxiv - Molecular Biology 2019Quote: All MKRN1 mutant plasmids were generated with the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2019Quote: ... All cloning was done using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB #E5520). PCRs were done using Q5® High-Fidelity DNA Polymerase (NEB #M0491).
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bioRxiv - Microbiology 2019Quote: ... Libraries were constructed using the NEBNext Ultra DNA Sample Prep Master Mix Kit (NEB), with minor modifications and a custom automated protocol on a Biomek FX (Beckman) ...
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bioRxiv - Genetics 2019Quote: The homologous repair constructs were created using the NEBuilder HiFi DNA Kit (NEB, E5520S) and contained 1,000 bases upstream of the first guide RNA target Cas9 site ...
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bioRxiv - Biophysics 2019Quote: ... Mutant constructs were generated using a site-directed mutagenesis kit (Q5, New England Biolabs) using primers described in the supplement ...
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bioRxiv - Biochemistry 2019Quote: We introduced mutations in phAPOBEC3B-HA using the Q5 Site-Directed Mutagenesis Kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We performed cDNA synthesis following standard protocols with the ProtoScript cDNA synthesis kit (NEB) using 1 µg of RNA for each sample ...
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bioRxiv - Genetics 2020Quote: ... cDNA was generated using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs). RT-qPCRs were performed on the Light Cycler 480II (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... The NEBNext Poly(A) mRNA magnetic isolation kit (New England Biolabs, Ipswich MA, E7490) was used to purify mRNA from the total RNA ...
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bioRxiv - Microbiology 2019Quote: ... For eight samples the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) and NextSeq (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... and mutations were made using the Q5 Site-directed mutagenesis kit (New England Biolabs). The BacMam vector was a kind gift from Professor Eric Gouaux (Vollum Institute).42 Briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... and used for cDNA library generation with the NEBNext Ultra kit (New England BioLabs), without enrichment ...
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bioRxiv - Cell Biology 2020Quote: ... using the Q5 quick mutagenesis kit (purchased from New England BioLabs, catalog number E0554s) and following the protocol provided by the manufacturer ...
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bioRxiv - Synthetic Biology 2021Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB cat# E7760L). 500 ng RNA was used at input ...
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bioRxiv - Genomics 2021Quote: ➢ Distinct from currently used length 8 barcodes from common library kits (Illumina TruSeq, NEB)
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was generated using Protoscript II First Strand cDNA Synthesis kit (New England Biolabs). cDNAs were diluted 1:20 and then qPCR was carried out in triplicate using the Luna Universal qPCR Master Mix (New England Biolabs) ...
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bioRxiv - Biochemistry 2021Quote: ... Resulting gRNA transcription was performed with HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocol and gRNA was purified and verified for concentration and RNA integrity ...
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bioRxiv - Immunology 2021Quote: ... Libraries were then constructed with the NEB Next Ultra II FS kit (E6177S, NEB) using the prime-seq specifications ...
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bioRxiv - Microbiology 2022Quote: ... Then Trizol was added before RNA was extracted using Monarch RNA Cleanup Kit (NEB), skipping the gDNA cleaning column steps ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA libraries were prepared using the NEBNext Ultra II DNA Library Prep kit (NEB) with the NEBNext Poly(A ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted using 5x107 cells and Monarch Total RNA Miniprep Kit (NEB) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... purified from 1.5% agarose and quantified with NEBNext Library Quant Kit for Illumina (NEB). Sequencing was performed on Illumina HiSeqV4 SR50.
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bioRxiv - Synthetic Biology 2021Quote: Total RNA was extracted from cell-free expression reactions with a kit (NEB #T2010), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product was purified using the Monarch PCR & DNA clean-up kit (NEB). All library preparation and sequencing was carried out at the Vanderbilt Technologies for Advanced Genomics (VANTAGE ...
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bioRxiv - Microbiology 2021Quote: ... Mutagenesis was performed in two steps using Q5 site-directed mutagenesis kit (NEB, USA) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: The pIGLR-2∷mCherry construct was generated with a Gibson assembly cloning kit (NEB) with the following four fragments ...
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bioRxiv - Genomics 2021Quote: ... Single-nucleotide point mutations were generated using a Q5 site-directed mutagenesis kit (NEB) and confirmed by Sanger sequencing ...
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bioRxiv - Biophysics 2020Quote: ... Mutagenesis of STEAP construct was performed using the Q5 Site-Directed Mutagenesis Kit (NEB). The primers used in this study are listed in Table S1 ...
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bioRxiv - Genomics 2020Quote: ... and end-repaired through NEBnext ultra II end repair kit (New England Biolabs, USA). Next ...
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bioRxiv - Microbiology 2021Quote: ... Fragmented DNA was end-repaired using the NEBNext Ultra I kit (New England Biolabs). The fragments were then ligated with an adapter ...
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bioRxiv - Biophysics 2021Quote: ... Mutants were generated by mutagenesis PCR using the Q5 Site-Directed Mutagenesis Kit (NEB), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: Complementation vectors were generated using NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Primers were designed using the online NEBuilder Assembly Tool with the following setting ...
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bioRxiv - Molecular Biology 2021Quote: ... The capped mRNAs were in vitro synthesized with HiScribe T7 ARCA mRNA Kit (NEB) and purified with an RNA Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: ... IVT was performed using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). Nuclease prime editor mRNA and pegRNA were purified using RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... Gel fragments of homozygous clones were extracted using Monarch Nucleic Acid Purification Kits (NEB) and submitted for Sanger Sequencing ...
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bioRxiv - Immunology 2020Quote: ... and gel fragments of clones were extracted using Monarch Nucleic Acid Purification Kits (NEB) and submitted for Sanger Sequencing ...
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bioRxiv - Microbiology 2020Quote: ... Derivatives of this plasmid were made using a Q5 site-directed mutagenesis kit (NEB). Single copy deaD-lacZ fusions were made by recombining the plasmid-borne fusions with modified λ phage ...
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bioRxiv - Cell Biology 2021Quote: ... The fragments were assembled using NEBuilder HiFi DNA Assembly Cloning kit (New England Biolabs) to yield the pPB-TRE Flag-wtATM plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... or if Gibson assembly was used with Gibson assembly kit #E2611 (New England Biolabs). Details on the construction of the plasmids used to create the different transgenes are shown in suppl ...
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bioRxiv - Genetics 2019Quote: ... total RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were prepared using the NEBNext Ultra DNA Library Prep kit (NEB, E7370) according to manufacturer’s instructions and submitted for paired-end Illumina sequencing on the HiSeq 2500.
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bioRxiv - Genomics 2021Quote: ... then libraries were prepared using the NEBNext DNA library prep kit (New England Biolabs). Final libraries were sequenced on a Novaseq platform (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... reverse transcription was performed using the ProtoScript II First Strand cDNA Synthesis Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Libraries were constructed using the NEBNext Ultra DNA Sample Prep Master Mix Kit (NEB) with minor modifications and a custom automated protocol on a Biomek FX (Beckman)25 ...
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bioRxiv - Genetics 2020Quote: Repair template plasmids were assembled from overlapping fragments using the NEBuilder HiFi Kit (NEB) per manufacturer instructions ...
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bioRxiv - Genetics 2020Quote: ... The variant was introduced using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). Cells were transfected with 6 μg of each plasmid using Lipofectamine 2000 (Invitrogen) ...