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Citations for New England Biolabs :
2301 - 2350 of 3344 citations for PD 1 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... were combined with RT primer mix [1 μl 250 ng/μl randomhexRT primer and 0.5 μl 10 mM dNTPs (NEB)] ...
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bioRxiv - Genomics 2019Quote: ... 0.5 ng of genomic DNA was digested with 20 µL of MspI master mix (1 µL of MspI (NEB), 2 µL 10x NEB CutSmart Buffer in a total volume of 20 µL ...
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bioRxiv - Genomics 2020Quote: ... before being resuspended in 1X ligase (1X T4 ligase buffer, 1:100 dilution of T4 DNA ligase NEB #M0202S) and incubated at room temperature for one hour ...
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bioRxiv - Molecular Biology 2021Quote: ... The annealed oligos were diluted 1:50 and then ligated into 50 ng NotI+BamHI-digested pBRα or pACλCI using T4 ligase (NEB), generating pS197 and pS198 ...
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bioRxiv - Synthetic Biology 2019Quote: ... 0.25 µl of T4 DNA ligase at 400 U µL−1 and 0.25 µl of SapI (NEB cat. R0569) at 10 U µL−1 ...
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bioRxiv - Microbiology 2020Quote: ... were carried out using specific primers (Supplemental Table 1) and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). After sequence verification ...
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bioRxiv - Molecular Biology 2019Quote: ... Bound RNA was eluted with 100 μl of Elution Buffer (5mM Tris-HCl, 1mM EDTA and 0.05% SDS) and 1 μl of Proteinase K (New England BioLabs) and incubated for 1.5 hour at 50°C ...
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bioRxiv - Developmental Biology 2020Quote: ... oligonucleotides for the HAS-1 or HAS-2 sgRNA guide sequences were phosphorylated using T4 PNK (NEB, Ipswich, MA) for 30 min at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... Stability from exonuclease I degradation was tested by incubating cssDNA phPB84 with exonuclease I in 1× exonuclease buffer (NEB) at 37°C for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR was performed on 0.5 – 1 ng of genomic DNA with the LongAmp Taq DNA Polymerase (New England Biolabs) using standard PCR reaction mix and long extension times (9 minutes at 65°C).
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bioRxiv - Microbiology 2019Quote: ... Cells were lysed in 100 µl lysis buffer (5 ml contain: 10 mM Tris-HCl pH 7.5, 4% SDS, 1 PhosSTOP tablet [Roche], a scoop of DNase I [NEB]) for 5 min at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... was incubated with 0.025 U of Nt.BbvC1 in a final volume of 100 µl of 1 X CutSmart buffer (New England Biolabs) to introduce sequence specific nicks in λDNA ...
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bioRxiv - Genomics 2020Quote: Cas9 protein and transcribed sgRNA were incubated for 10 min at room temperature in reaction buffer containing 1× NEB buffer 3.1 (NEB Biolabs) supplemented with 1 mM DTT ...
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bioRxiv - Molecular Biology 2019Quote: ... Pooled PCR products (1 μg) were digested with the NEBNext dsDNA fragmentase kit (New England BioLabs, Inc., Ipswich, MA). Fragmented DNA was end-repaired with the NEBNext End Repair Module (New England BioLabs ...
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bioRxiv - Synthetic Biology 2020Quote: ... and subsequently mixed at a 6:1 ratio with the digested backbone in reactions containing T4 DNA ligase (NEB).
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bioRxiv - Genetics 2019Quote: ... Data were reported as the nearest kilobase using the 1 kb DNA ladder (New England Biolabs, Beverly, MA, USA) as a reference.
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bioRxiv - Molecular Biology 2019Quote: ... Beads were washed twice with ELBS and suspended in 20 μL PMP buffer supplemented with 1 mM MnCl2 (NEB). 200 units λ protein phosphatase (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μg ml−1 of template DNA was mixed with 1000 U ml−1 of T7 polymerase and 1.5 mM of each rNTP (New England Biolabs) in 40 mM Tris-HCl ...
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bioRxiv - Genetics 2020Quote: Total RNA was extracted using the standard hot acid phenol method and treated with DNase 1 (New England Biolabs). qRT-PCR was performed using amfiRivert cDNA synthesis Platinum Master Mix from Gendepot ...
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bioRxiv - Genomics 2021Quote: ... 1% of the cDNA was used for each qPCR reaction performed with the Luna Universal qPCR Mastermix (NEB M3003) and the PCR primers listed (Supplementary Table1) ...
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bioRxiv - Genomics 2020Quote: ... Primer mixes were diluted 1:25 and 1μl of each mix was used for overlap-extension PCR using Phusion (NEB). Four 50μl reactions for each mix were performed using cycling conditions 98°C 1min ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... UK) were then synthesized through in vitro transcription of 1 mg template with T7 polymerases (M0251, New England Biolabs). The sections were fixated in 4% PFA ...
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bioRxiv - Microbiology 2021Quote: Protein samples were generated by collecting the pellet for 1 ml of cells at OD600 ∼ 1.0 and lysing with 100 µl 1X SDS sample buffer (New England Biolabs) and DTT by boiling for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... RHA PCR products were ligated into pAAV p21 vector by Gibson assembly at a ratio vector:inserts of 1:2:2 using T4 DNA ligase (NEB). All constructs were checked by sequencing before transfection into cells ...
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bioRxiv - Bioengineering 2021Quote: ... Resulting amplicons were denatured and reannealed in a thermocycler before nuclease reaction using T7 Endonuclease 1 (New England Biolabs) at 37C for 20min ...
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bioRxiv - Bioengineering 2020Quote: ... Constriction was performed by diluting a library to approximately 8 fM (~5,000 molecules/μl) in salmon sperm DNA (1 ng/μl, New England Biolabs) in low-binding tubes (LoBind ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μg of “heavy” or “light” THP-1 protein extract were dephosphorylated with 50 U of Antarctic phosphatase (NEB) in Antarctic phosphatase buffer (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The fragment was ligated into the pLSV101 vector (3:1 molar ratio) with T4 DNA ligase (New England Biolabs) (16 °C overnight) ...
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bioRxiv - Biochemistry 2020Quote: ... Remodeling reactions were started by adding 10 μl SGD chromatin corresponding to about 1 μg DNA assembled into nucleosomes and terminated by adding 0.8 Units apyrase (NEB) followed by incubation at 30 °C for 30 min.
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 1 mM MnCl2 was used to remove the phosphorylation on the peptides and Staurosporin (NEB, Hitchin, UK) to occupy the active site pocket ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5 μl of reaction was added to a tube containing 5 μl replication buffer and 1 μl MseI (NEB) for 3 min ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µl was added to a 9 µl Luna® Universal One-Step RT-qPCR reaction (NEB, Cat# E3005L). RT-qPCR was carried out to manufacturer specifications in a CFX96 Touch Real Time PCR Machine (Bio-Rad) ...
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bioRxiv - Plant Biology 2021Quote: ... High fidelity PCR was conducted on the abcg43-1 line using the Q5 High-Fidelity 2X Master Mix (NEB). PCR products were purified and extracted from a 1% agarose gel using the QIAquick Gel Extraction Kit (QIAGEN) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 96-well plates were centrifuged again at 3000 x g for 10 min and 1 µL of the supernatant was used in 12.5 µL PCR reactions with Taq polymerase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... before incubation during 6 min in 50μl of PNK reaction mix (1x PNKT, 1 mM ATP and 0.05 U/ml T4 PNK 3′phosphatase minus (NEB) in a thermomixer at 37°C and 1400rpm ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from 0.5-1 µg of RNA per sample using Protoscript II RT and Random Primer Mix (New England Biolabs). qPCR reactions were performed on cDNA using iQ SYBR Green supermix (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... 107 epimastigotes were electroporated with 2.5 µg of pTRIX2-GFP plasmid (Fig 1 A) linearized with AscI and SacI (NEB). The plasmid had been derived from pTRIX-REh9 [28] ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 1:8 dilution Cas9 protein (New England Biolabs, cat. M0386M; diluted in buffer B, New England Biolabs, cat. B802S) and 1:40 dilution of phenol red (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized from 1 μg total RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB) with oligo-dT priming according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... The coupling reaction was performed at 25 °C for 1 h and followed by addition of 0.2 unit/mL apyrase (New England Biolabs). After additional 1 h at 25 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were dialyzed with water on silica membranes (0.025 μm pores) for 1 hour before transformed into DH10β cells (New England Biolabs). Library sizes of at least 100,000 colony-forming units (CFU ...
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bioRxiv - Biochemistry 2022Quote: Native 3’ end extension assays experiments where set-up as describe in the D-loop assays with the exception that dNTP’s (1 mM) and Klenow (exo-)(NEB) were added after 10 minutes of D-loop formation ...
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bioRxiv - Genetics 2022Quote: ... The human codon-optimized Cas9 gene (Supplementary file 1) was synthesized by HuaGene (Shanghai, China) and cloned into the Nme2Cas9_AAV backbone by the NEBuilder assembly tool (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 25mM Tris-HCl) and then lysed using TD/1% NP-40/RVC (Ribonucleoside-Vanadyl Complex, NEB, Cat. No. S1402) in the presence of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher ...
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bioRxiv - Developmental Biology 2020Quote: ... standard PCR reactions were performed on 1 ul of cDNA using Q5 High-Fidelity DNA Polymerase (New England Biolabs) and each of the variant PCR primer pairs independently ...
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bioRxiv - Plant Biology 2021Quote: ... We sent 300ng of DNA in 20μl to LGC Genomics GmbH (Germany) where genomic DNA were digested with 1 Unit MslI (NEB) in 1 times NEB4 buffer in 30 μl volume for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were treated for 15 minutes at 37°C with 1 μM BG-SS-649 (Marzook et al., 2021) (a gift from Dr Ivan Corrêa Jr, New England Biolabs) in complete media to reversibly label surface SNAP-tagged receptor populations ...
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bioRxiv - Molecular Biology 2021Quote: ... Two separate 8-µl aliquots of beads derived from each strain were treated with 1 µl lambda phosphatase (NEB) in 10 µl reactions containing ...
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bioRxiv - Microbiology 2021Quote: ... the THN gene was ligated to pICH41021 in a 3:1 molar ratio using T4 Ligase (New England Biolabs) at 4°C overnight and transformed by heat shock into chemically competent E ...
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bioRxiv - Molecular Biology 2019Quote: ... Ncas_Int679_For 5′ GGCAAATTTGTATGAGGGATAAA and Ncas_Int679_Rev 5′ TAATTCGATTACGTTAGCTGTT) and cloned into pRS403-pGAL1-hpSC_URA3 (1) using PsiI and NaeI restriction enzymes (New England Biolabs, NEB). In addition ...