Labshake search
Citations for New England Biolabs :
2301 - 2350 of 9715 citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... followed by site-directed mutagenesis using the Q-5 kit (New England Biolabs) in order to generate point mutations into MIC1 (MIC1-T126A/T220A ...
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bioRxiv - Biochemistry 2019Quote: FXN variants were constructed using the Q5® Site-Directed Mutagenesis Kit (NEB) and confirmed by sequencing of plasmid DNA and intact mass spectrometry of purified proteins ...
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bioRxiv - Genetics 2021Quote: ... PCR amplicons were purified using the Monarch DNA Gel Extraction Kit (NEB, T1020S) and subjected to sequencing.
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bioRxiv - Genetics 2020Quote: ... Site directed mutagenesis using the Q5 Site-Directed Mutagenesis kit (New England Biolabs) and AP2S1 specific primers (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760). A total of 20 libraries were constructed [including ...
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bioRxiv - Genetics 2020Quote: ... samples were purified by the Monarch PCR & DNA Cleanup Kit (New England Biolabs). For the library preparation ...
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bioRxiv - Cell Biology 2021Quote: ... and blunt and phosphorylated ends were generated using the Quick Blunting kit (NEB), before removal of DNA fragments under 100 bp using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Immunology 2021Quote: ... Luna Universal Probe One-step RT-qPCR kit (New England Biolabs, Ipswich, MA) was used to amplify the envelope (E ...
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bioRxiv - Genomics 2020Quote: ... We used the NEBNext Ultra DNA Library Prep Kit (New England Biolabs, USA) to generate Illumina sequencing libraries from sheared DNA samples ...
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bioRxiv - Immunology 2021Quote: ... and then transcribed with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) [23] ...
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bioRxiv - Plant Biology 2020Quote: ... The NEBNext Ultra™ II FS DNA Library Prep Kit (New England Biolabs) was used for fragmentation/adaptor ligation and indexing of samples ...
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bioRxiv - Bioengineering 2020Quote: ... and polyadenylated using HiScribe ARCA T7 mRNA Kit (with tailing) (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... C4 domain sequence was removed with the Q5 site-directed mutagenesis kit (NEB). Mouse prolyl 4-hydroxylase alpha- and beta-chain (P4HA1 and P4HB ...
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bioRxiv - Cancer Biology 2020Quote: ... Site-directed mutagenesis was performed using Q5 Site-Directed Mutagenesis Kit (NEB, E0552S). Retrovirus was generated by transfection of Phoenix cells with 3:1 ratio of target plasmid ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was prepared using LunaScript™ RT SuperMix Kit (New England Biolabs, E3010S). Real-time PCR was performed using Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... Point mutations were made using the Q5® Site-Directed Mutagenesis Kit (NEB). Expi293FTM cells were maintained in Expi293 Expression medium and cultured under 7% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Expression plasmids were assembled using the Gibson assembly kit (New England Biolabs, U.S.A) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cGTP_for and cGTP_rev using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufactureŕs protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and NEBNext Ultra II RNA Library Prep Kit for Illumina® (NEB, #E7775) was then used to prepare libraries for paired-end (2×150 bp ...
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bioRxiv - Microbiology 2021Quote: ... RNA libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit (NEB). Quality of the total RNA ...
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bioRxiv - Biochemistry 2021Quote: ... and point mutations were performed using the Q5 site-directed mutagenesis kit (NEB). Proteins were expressed in E ...
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bioRxiv - Biochemistry 2020Quote: ... cDNA was obtained using LunaScript™ RT SuperMix Kit (New England Biolabs, USA) and qPCR reactions were performed using Luna® Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Plant Biology 2021Quote: ... These DNA fragments were assembled using NEBuilder HiFi Assembly Cloning Kit (NEB, E2621) into ApaI-digested pJN105ΔpBAD ...
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bioRxiv - Systems Biology 2021Quote: ... Fragments were gel purified using the NEB Monarch gel extraction kit (NEB T1020S). Following gel extraction ...
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bioRxiv - Plant Biology 2020Quote: ... or NEB Next® Ultra™ RNA Library Prep Kit (NEB, Ipswich, USA). Illumina sequencing of mRNA was performed with 150 bp paired-end reads at the Cologne Center for Genomics (CCG ...
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bioRxiv - Plant Biology 2021Quote: ... using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified and in vitro transcribed using T7 HiScribe kit (NEB). Transcribed RNA products were run on a 5% acrylamide TBE-urea gel and visualized with 0.02% methylene blue in 1x TBE ...
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bioRxiv - Microbiology 2021Quote: ... Sheared DNA was repaired using NEBNext blunt-end repair kit (New England Biolabs) and purified using Monarch PCR clean-up kit (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... HiScribe™ T7 High Yield RNA Synthesis Kit (New England BioLabs, catalog# E2040S) was used to make the crRNA/gRNA with the above prepared DNA template according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was then extracted using the Monarch gDNA Purification Kit (NEB T3010S) following the standard protocol but with elution using 85 μL 25 mM K3BO3 at pH 7.0.
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bioRxiv - Genomics 2020Quote: ... Total RNA was extracted following the Monarch Total RNA Miniprep Kit (NEB #T2010S) protocol with minor modifications ...
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bioRxiv - Microbiology 2021Quote: ... Ribosomal RNA (rRNA) was removed by NEBNext rRNA Depletion Kit (New England BioLabs) according to manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... Addgene plasmid # 46888] using NEBuilder HiFi DNA assembly cloning kit (New England Biolabs) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... Restriction enzymes and Quick Ligation kits were obtained from New England Biolabs (NEB). Sequences were verified by Sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2020Quote: ... the sequencing libraries were prepared using Illumina DNA prep kit (New England BioLabs). The library fragment sizes were measured using agarose gel electrophoresis and the concentrations using Qubit dsDNA HS Assay Kit (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Adenylation of R1R was done using 5’ DNA Adenylation kit (New England Biolabs) according to manufacturer’s instructions and cleaned with Oligo Clean & Concentrator (Zymo Research ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was purified using the Monarch PCR and DNA cleanup kit (NEB) to remove unincorporated dATP ...
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bioRxiv - Microbiology 2021Quote: ... was generated by using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs), the plasmid pCDNA3.1-SARS2-Spike and the following mutagenic oligonucleotide primers ...
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bioRxiv - Molecular Biology 2020Quote: ... and reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (NEB, E6560), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Final expression constructs were assembled with the HiFi Assembly kit (New England Biolabs), using amplicons generated with Q5 polymerase from the promoter and gene as two fragments ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was synthesised using the LunaScript RT SuperMix Kit (New England Biolabs E3010) and gene expression was analyzed by RT-qPCR on QuantStudio 6 (Applied Biosciences ...
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bioRxiv - Biophysics 2020Quote: ... the linearized T270 was ligated using the Quick LigationTM Kit (New England BioLabs) at room temperature for 1 hr followed by additional incubation at 4°C for overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... and complementary DNA was synthesized by using the ProtoScript kit (New England BioLabs). Quantitative real-time PCR was conducted by using sequence-specific primers (CCRL2 forward 5’-CTTCGAGAAAAACGTCTC-3’ and reverse 5’-CATCATATTCATCCTCTGGTG-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... were built using either Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S) or In-Fusion HD Cloning Plus (Clonetech ...
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bioRxiv - Cell Biology 2021Quote: ... using BamHI/EcoRI sites with an NEBuilder HiFi DNA Assembly Cloning Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was then extracted using the Monarch gDNA Purification Kit (NEB T3010S) following the standard protocol but with elution using 175 µL 25 mM K3BO3 at pH 7.0.
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bioRxiv - Genomics 2020Quote: ... Plasmid DNA was purified from liquid cultures using DNA miniprep kit (NEB #T1010).
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bioRxiv - Biophysics 2022Quote: ... The final product was purified by Monarch RNA Cleanup kit (New England Biolabs), eluted in water ...
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bioRxiv - Molecular Biology 2022Quote: ... NGS libraries were prepared using NEBNext Ultra II DNA Library Prep kit (NEB). DNA sequencing was performed on Illumina MiSeq with the 250+250 bp paired-end protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... A NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs) was used according to the manufacturer’s instructions for sample library preparation ...