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Citations for New England Biolabs :
2251 - 2300 of 4954 citations for 6 Phenyl 1H imidazo 1 2 b pyrazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR was performed with 2 μl of the reverse transcription positive reactions and Phusion High Fidelity DNA polymerase (NEB, Ipswich, MA) following manufacturer’s protocols on an Applied Biosystems 2720 Thermal Cycler ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were washed 2 times with EB2 without NaF or PhosStop and one time with λpp buffer (New England Biolabs, Ipswich, MA) before resuspension in 40 μl of λpp buffer ...
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bioRxiv - Plant Biology 2024Quote: ... mCherry-2-L and the PIF5 coding sequence into pJHA212G80 containing the rbcS terminator using Gibson Assembly (New England Biolabs, Ipswich, MA). The resulting construct was transformed into pif5-3 using Agrobacterium-mediated transformation ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 2 ng of RNA per sample using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420). Libraries were pooled and sequenced using a P3-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... containing a 5′-Biotin-PC group and a 3’-OH (Sup. Table. 2) were ligated to the 5′ end of RNAs using T4 RNA ligase (NEB, Cat #M0204S) for 3 hours at 37°C ...
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bioRxiv - Genomics 2024Quote: ... after which 68 µL RNase-free H2O was added with 10 µL DNase I Buffer and 2 µL DNase I (New England Biolabs, Ipswitch, MA). The resulting mix was incubated for 15m at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... 100 ng of template DNA was used in a 2 hour in vitro transcription reaction using the HiScribe T7 High Yield RNA (New England Biolabs, Cat. E2040S) kit according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... spanning the whole SARS-CoV-2 genome were amplified using the high-fidelity proofreading enzyme Q5® High-Fidelity DNA Polymerase (NEB, M0491L) in a 25 µL reaction volume using respective primers (fig ...
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bioRxiv - Synthetic Biology 2023Quote: BigBlocks were PCR-amplified with dedicated primer pairs in a 25.0 μL PCR reaction mix composed of 0.25 μL Q5® Hot Start High-Fidelity DNA Polymerase 2 U/μL (NEB® M0493), 5.0 μL Q5® 5X buffer (NEB® B9027) ...
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bioRxiv - Microbiology 2023Quote: ... was amplified from JE2 using the primer pair 3810/9647 and the snap-tag coding sequence amplified from pSNAP-tag (T7)-2 (New England Biolabs, NEB; Supplementary Information) using the primer pair 9631/9632 ...
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bioRxiv - Immunology 2023Quote: ... PCR was performed with 2 ng of library input per reaction and 7 cycles of amplification using Q5 DNA polymerase (New England Biolabs, catalog # M0491S). Libraries were quantified using a Qubit 4 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were selected for cDNA target fragments of 200 bp on 2% low-range Ultra agarose followed by PCR amplification using Phusion DNA polymerase (NEB, United States) for 15 PCR cycles ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
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bioRxiv - Microbiology 2023Quote: ... 9 DNA fragments encoding the partial genome of SARS-CoV-2 were prepared by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Cat# M0491S). A linker fragment encoding hepatitis delta virus ribozyme ...
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bioRxiv - Molecular Biology 2024Quote: ... The NicE-view labeling mix was based on previously published protocols24–26 and consisted of 1x NEBuffer 2 (NEB, Ipswich USA, #B7002S), 30 µM dGTP (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Adapters were subsequently ligated to deacylated and demethylated RNA templates using a truncated KQ T4 RNA ligase 2 (New England Biolabs, cat# M0373L) for 1 hour at 25°C with 20% PEG8000 (New England Biolabs) ...
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bioRxiv - Genetics 2024Quote: ... sgRNA complementary to the central part of the hACE2 (Table 2) was synthesized with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, USA). Library was loaded onto the FLO-MIN106 flow cell and sequenced with GridION (Oxford Nanopore ...
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bioRxiv - Genomics 2024Quote: ... 0.5 µM GAT-7N primers (5′-GTG AGT GAT GGT TGA GGT AGT GTG GAG NNN NNN N) and 2 units/µl DeepVent® (exo-) DNA polymerase (New England Biolabs, M0259L) and incubated for 11 cycles in the BioRad thermocycler ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cat. No. B6002S]; 2.5 µl 100 µM gRNA [ordered as a synthesized oligo]; 2 µl 100 µM LbaCas12a [NEB, Cat. No. M0653T]) was incubated for 5 minutes at room temperature.
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bioRxiv - Genomics 2024Quote: ... 0.5 µM GAT-7N random hexamer primers with an adapter sequence and 2 units/µl DeepVent® (exo-) DNA polymerase (New England Biolabs, M0259L). DNA was further amplified as in the pre-amplification step above ...
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bioRxiv - Molecular Biology 2024Quote: ... were ligated to the 3’ ends of total RNA from EVs and cellular samples isolated from an RN cell line using a truncated KQ T4 RNA ligase 2 (New England Biolabs, cat# M0373L) for 1 h at 25°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA was diluted to 0.2 ng.μl-1 and 1 ng was analysed by qPCR using Luna Universal qPCR MasterMix (NEB) with 500nM of primers ...
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bioRxiv - Genomics 2020Quote: ... before and in between the following procedures: (1) 3′ adapter ligation with T4 RNA Ligase 1 (NEB, M0204L), (2 ...
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bioRxiv - Cell Biology 2020Quote: ... Single-stranded DNA primers and primer dimers were digested using 1 unit μL−1 ExoI (New England Biolabs) and 1 U μL−1 HinFI (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... but instead of ATP 1 mM MnCl2 was added and 1 μl (400 U) of λ phosphatase (NEB) was used instead of PKR.
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bioRxiv - Cell Biology 2021Quote: ... with additionally 1 μl oligo-dT primers and 1 μl of dNTP mix (NEB Biolabs, Ipswich, MA, USA). Further ...
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bioRxiv - Cell Biology 2021Quote: ... with additionally 1 μl oligo-dT primers and 1 μl of dNTP mix (NEB Biolabs, Ipswich, MA, USA). Further ...
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bioRxiv - Microbiology 2022Quote: ... RNA integrity was confirmed by mixing samples 1:1 in 2X RNA loading dye (B0363S; New England Biolabs) and separating by 0.8 % (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (1:10.000, used with 1:10.000 secondary horseradish peroxidase-conjugated anti-mouse antibody; NEB).
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bioRxiv - Bioengineering 2021Quote: ... 1:15 and 1:30 (PEI-AuNCs:siRNA)) were incubated with RNase H (10μg/ml, New England BioLabs, M0297L) at 37°C for 30 minutes ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Each reaction was visualised on a 1 % agarose gel using a 1 kb DNA ladder (New England Biolabs) and a Lambda ladder (Roche ...
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bioRxiv - Microbiology 2022Quote: ... 1-20 μg of the Env protein was mixed with 1 μl of Glycoprotein Denaturing Buffer (NEB, 10×) and H2O (if necessary ...
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bioRxiv - Molecular Biology 2022Quote: ... The products were resolved in 1% agarose gels along with 1 kb Plus DNA Ladder (New England Biolabs) and visualized using SYBR™ Safe DNA Gel Stain (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µl 10x BSA (diluted 1:10 with Milli-Q water from a 100X stock, New England Biolabs), and 1 µl of each enzyme used as described in Fig ...
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bioRxiv - Neuroscience 2023Quote: ... column purified and ligated at a 1:1 vector to insert ratio using the QuickLig Kit (NEB #M2200S). The ASCL1 stop codon was subsequently mutated using the QuickChange II-XL site-directed mutagenesis kit (Agilent #200523 ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2) and either 25 nM NudE.1 WT or NudE.1 E64,65Q or NudC WT (NEB). For NAD spike-in kinetics ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 µl 100× Protease inhibitor cocktail and 1 µl of CviKI-1 (5 U/100,000 nuclei, NEB R0710S) were added ...
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bioRxiv - Plant Biology 2024Quote: ... we used a 1:1 mix of Low Range ssRNA Ladder (New England Biolabs™, catalog no. N0364S) and 14-30 nt ssRNA Ladder Marker (Takara™ ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μl ligation enhancer (New England BioLabs), 0.9 μl nuclease-free water and 0.1 μl duplexed adapter (100 μM ...
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bioRxiv - Cell Biology 2020Quote: ... Index Primers Set 1 (New England Biolabs). P5/P7 PCR fragments were purified with Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Cell Biology 2020Quote: ... T7 Endonuclease-1 was purchased from NEB, US (Cat ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 uL of PNK enzyme (NEB M0236S), and 2 uL of 1mM ATP to total 10 uL and incubated at 37 C for 30 minutes followed by inactivation by heating to 65 C for 20 minutes.
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bioRxiv - Developmental Biology 2021Quote: ... and 1 U of Klenow fragment (NEB) at 20°C for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... 1 μl of NEBNext Ligation Enhancer (NEB), and 2 μl of 1.5 μM NEBNext adaptors for Illumina sequencing (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... and 1 µl of PNK enzyme (NEB), carried out at 37°C for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... 1 µl of 10x PNK buffer (NEB), 1 µl of rSAP enzyme (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 unit/µl Murine RNase Inhibitor (NEB), protease inhibitor (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 unit/µl Murine RNase Inhibitor (NEB), protease inhibitor (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 unit/µl Murine RNase Inhibitor (NEB), protease inhibitor (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 unit/µl Murine RNase Inhibitor (NEB), protease inhibitor (Pierce ...