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Citations for New England Biolabs :
2201 - 2250 of 10000+ citations for Rat Activity Dependent Neuroprotector Homeobox Protein ADNP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The DNA encoding the EGFP-fusion proteins and the P2A peptide was inserted by Gibson assembly using NEBuilder HiFi DNA assembly master mix (New England BioLabs, Ipswich, MA, USA). The HeLa Flp-In T-REx K44A Dynamin2-EGFP-P2A-Caveolin1-mCherry construct was obtained by in vitro mutagenesis of the pcDNA/FRT/TO/Dyn2-EGFP-P2A-Caveolin1-mCherry construct exchanging lysine at position 44 to an alanin using these primers ...
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bioRxiv - Developmental Biology 2020Quote: ... A total of 863 Bicyclus embryos were injected with 300 ng/µl of guide and 300 ng/µl of Cas9 protein (NEB; Cat. no.: M0641) mixed together in equal parts (total volume = 10 µl ...
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bioRxiv - Developmental Biology 2019Quote: ... The bound protein (~42kDa, 360 amino acids) was released from the MBP tag by cleavage with Factor Xa (New England Biolabs, Massachusetts, United States) for 24 hours at 4°C in 20mMTris pH 7.2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The synthetic genes encoding WT human TRIM28 with an N-terminal maltose binding protein (MBP) affinity tag (UniProt: Q13263) were subcloned to the pMAL-p2X vector (New England Biolabs, Beverly, MA, USA).
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bioRxiv - Bioengineering 2023Quote: ErCas12a (1 μg) or LbCas12aU (1 μg) protein and 1 μg crRNA were assembled in 1X Nuclease Reaction Buffer (New England BioLabs, Ipswich, MA, USA) at room temperature for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... corresponding to a portion of PfRNF1 (RP2; Fig. 1A) was expressed as a maltose-binding fusion protein using the pMAL™c5X-vector (New England Biolabs, Ipswich, USA). To this end ...
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bioRxiv - Microbiology 2021Quote: The rem allele was amplified from a cloning construct (primer sequences in Table S4) and ligated into pTYB12 (NEB IMPACT system of protein purification), resulting in an N-terminal intein fusion to Rem ...
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bioRxiv - Microbiology 2021Quote: ... MNase activity was quenched by addition of EGTA and samples were subsequently pre-cleared by addition of 40 µl Protein A Magnetic Beads (New England Biolabs, Ipswich, MA, United States) and rotating at 4°C for 60 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of both N-linked and O-linked glycans was performed by incubation with five different glycosidases: PNGaseF (New England Biolabs, 125 U/μg protein), O-glycosidase (New England Biolabs ...
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bioRxiv - Bioengineering 2022Quote: ... Processing the samples for proteomics was then continued using a 10 kDa filter aided sample preparation protocol previously published with minor modifications.[30] The proteins were digested using Lys-C enzyme (P8109S, New England Biolabs, MA, USA; 1:50) and then with trypsin (V5111 ...
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bioRxiv - Biophysics 2019Quote: ... The resulting product (typically 50 – 100 μL of 5 – 20 μM of protein) was mixed with an equimolar amount of SNAP-Surface 549 (New England Biolabs; 1 mM in DMSO) and incubated at room temperature for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... aliquots of the same protein extract were separated and treated with 200 U of Lambda Phage Phosphatase (#P0753S, New England Biolabs, Ipswich, Massachusetts, United States), as specified by the manufacturer ...
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bioRxiv - Genetics 2021Quote: ... 5 μg of MBP-Rif2 or MBP-Rif2-min (see recombinant protein preparation) was incubated for 1 h at 4 °C with amylose resin (New England Biolabs, 50 μl per reaction). The resin with the immobilized MBP-Rif2 variants was washed 5 times with 1 ml wash buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... when OD600 reached 0.5 for 4 h at 37 °C and then they were purified using amylose resin column (New England Biolabs, for MBP related proteins purification) or Ni-NTA agarose column (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: Injections into fertilized eggs for knockout experiments were done using 50ng/µl of each sgRNA independently and 80nM of NEB EnGen Spy Cas9-NLS protein (New England Biolabs France Genopole, Evry, France), pre-incubated at room-temperature for 15 minutes (Hill et al. ...
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bioRxiv - Plant Biology 2020Quote: ... ChIP DNA was purified with the Monarch PCR & DNA Cleanup Kit (NEB). Libraries were prepared using ThruPLEX DNA-seq Kit (Rubicon Genomics ...
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bioRxiv - Plant Biology 2020Quote: ... were mixed for DNA assembly using the Golden Gate assembly kit (NEB).
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated using NEBNext Ultra II DNA Library Prep Kit (NEB), modified for CUT&RUN as previously described(54) ...
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bioRxiv - Cell Biology 2019Quote: ... then purified with Monarch PCR & DNA Purification Kit (New England BioLabs, T1030S) followed by T4 DNA Ligase reaction (New England BioLabs ...
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bioRxiv - Cell Biology 2020Quote: ... and the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB #7760). The libraries were sequenced using Next-Seq with single-end ...
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bioRxiv - Cell Biology 2020Quote: ... we used the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S) and pDONR221-GEM1 to make pDONR221-GEM1(S19N) ...
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bioRxiv - Cell Biology 2020Quote: ... Ligations were completed using the Quick Ligation Kit (New England Biolabs, M2200) according to manufacture protocol ...
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bioRxiv - Immunology 2021Quote: ... Small RNA library preparation was completed using NEBNext Small RNA kit (NEB) following manufacturer’s instructions and applying the low input protocol modifications ...
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bioRxiv - Genetics 2021Quote: ... Cloning was done using Gibson Assembly® Cloning Kit (New England Biolabs). The final product was amplified as a linear construct and confirmed by Sanger sequencing ...
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bioRxiv - Genetics 2021Quote: ... and quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs). The quantified pool was hybridized at a final concentration of 2.2 pM and sequenced paired-end on the Illumina NextSeq 500 platform using a High-Output v2.5 flowcell and 2×151c read length.
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bioRxiv - Genomics 2020Quote: ... The Monarch Total RNA Miniprep kit (New England Biolabs Inc, Ipswich, MA) was used for extraction ...
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bioRxiv - Microbiology 2020Quote: ... using the HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA, USA). pBOMBL contains a modified ribosome binding site to reduce leaky expression ...
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bioRxiv - Microbiology 2019Quote: ... We prepared sequencing libraries with NEBNext Ultra DNA Library Prep kits (NEB) and sequenced them on an Illumina NextSeq with 2×150 paired end reads (mid-output run ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Digested DNA was purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... twelve-cycle PCR reactions (Phusion High Fidelity PCR kit, New England Biolabs), using primers that added Flow Cell binding sequences ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were produced using the EnGen® sgRNA Synthesis Kit (NEB, E3322S). Primers are given in Table S2.
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bioRxiv - Neuroscience 2022Quote: In vitro transcription was performed using HiScribe Quick T7 kit (NEB E2050S) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... competent cells and were purified using NEB Monarch Miniprep Kits (NEB, Canada) as per manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... After electrophoresis and gel extraction by Monarch DNA Gel Extraction Kit (NEB), the PCR product was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... In vitro transcription was performed using HiScribe Quick T7 kit (NEB E2050S) following the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2021Quote: ... using HiScribe™ T7 High yield RNA Synthesis kit (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... using the Luna Universal One-Step RT-qPCR kit (New England BioLabs) and primer sets validated in our lab (Supplemental Table 5) ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared using the NEBNext Ultra II kit (New England Biolabs, Ipswitch ...
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bioRxiv - Molecular Biology 2020Quote: This oligonucleotide was adenylated using the 5’ DNA adenylation kit (NEB, E2610S) as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... The linkers were pre-adenylated with a 5’ DNA Adenylation kit (NEB), and then used for the ligation reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq libraries were prepared with NEBNext UltraDirectional kit (New England Biolabs). Libraries were pooled and sequenced on Illumina HiSeq 2500 instrument ...
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bioRxiv - Molecular Biology 2021Quote: ... NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... digestion and then in vitro transcribed using HiScribe T7 Transcription Kit (NEB). The RNA transcripts were extracted using Trizol (Thermo ...
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bioRxiv - Microbiology 2021Quote: ... or NEBNext UltraII DNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was performed using a HiSeq2500 sequencer (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently purified with Monarch RNA Cleanup Kit (NEB, cat. No. T2040).
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bioRxiv - Neuroscience 2021Quote: ... Isolated transcripts were ligated into pEGFP-C2 using Quick Ligation Kit (NEB), cloned into DH5α ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... and minipreps were performed using the Monarch Plasmid Miniprep Kit (NEB T1010L). NEB 5-alpha Competent E ...
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bioRxiv - Microbiology 2019Quote: ... and quantified with the NEBNext Library Quant Kit Protocol (New England BioLabs). The NextSeq 500/550 High Output Kit (300 cycles ...
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bioRxiv - Genetics 2020Quote: ... the DSB ends were blunted with NEB’s Quick Blunting Kit (NEB #E1201) according to the manufacturer’s instructions in a final volume of 100 μl for 60 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... and quantified using the NEBNext Library Quant Kit for Illumina (NEB, #E7630). After pooling ...