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Citations for New England Biolabs :
2201 - 2250 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) was used for the following RNA-seq library preparation according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using NEB Monarch RNA prep kit (New England BioLabs). The RNA concentration and RIN (RNA integrity number ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were purified using Monarch DNA Gel Extraction Kit (New England Biolabs). Both the vector and the complete insert were digested by BsmBI (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... before purifying again using an NEB RNA clean and concentrate kit (NEB #T2030).
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bioRxiv - Synthetic Biology 2024Quote: ... Selections were then purified using the Monarch® PCR & DNA Cleanup Kit (NEB), eluting in 10 µl of the Monarch® DNA Elution Buffer ...
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bioRxiv - Genomics 2024Quote: ... followed by purification with a Monarch® PCR & DNA Cleanup Kit (NEB®). Standard experimental conditions ...
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bioRxiv - Genomics 2024Quote: ... Further DNA was purified using Monarch® Genomic DNA Purification Kit (NEB T3010S) according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... and quantified using a NEBNext® Library Quant Kit for Illumina® (NEB) on a QuantStudioTM 5 Real-Time PCR System (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted using the bacteria rRNA depletion kit (New England Biolabs), with the addition of human rRNA depletion beads for the samples containing S ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were purified using a Monarch PCR Cleanup Kit (New England Biolabs) and prepared for nanopore long read sequencing using the Ligation Sequencing Kit (Oxford Nanopore Technologies ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using the Monarch PCR and DNA Cleanup Kit (New England Biolabs). 200 ng of purified product was used in a 20 µL IVT reaction using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... Dissolved peptides were incubated with antiKcr antibody-coated agarose beads (Catalog No. PTM-503, PTM Biolabs, Hangzhou, China) overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... overnight at 4°C followed by Alexa Fluor 488 goat anti-rabbit secondary antibody (New England Biolabs, UK) for 1h ...
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bioRxiv - Physiology 2020Quote: ... and analysed by Western blotting using antibodies for TCF4/TCF7L2 (C48H11) (#2569, 1:500, Cell signalling, NEB, UK), phospho-AKT (#9271 ...
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bioRxiv - Cell Biology 2021Quote: SNAP-GCGR was detected with an anti-SNAP-tag rabbit polyclonal antibody (P9310S, New England Biolabs, 1/500) followed by goat anti-rabbit IgG H&L HRP (ab6271 ...
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bioRxiv - Immunology 2022Quote: The paired antibody VH/VL chains were cloned into Igγ and Igk expression vectors using T4 ligase (NEB). Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 million permeabilized cells (without any antibodies bound) were incubated with 4 units of Dam enzyme (NEB, M0222L) during the activation step ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... was detected by addition of 1 nM Europium-labeled anti-p53 phosphoserine 15 antibody (New England Biolabs/ Cisbio) and 40 nM streptavidin-conjugated APC (Prozyme ...
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bioRxiv - Plant Biology 2024Quote: ... The beads were then boiled in SDS-loading buffer for subsequent western blotting using MBP antibodies (NEB, E8032S). Histone peptides utilized in this study were synthesized by EpiCypher and included H3 (1–32 aa) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibody rabbit anti-c-Fos (1:3000, New England Biolabs cat. No. 2250S) for 24 hours at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Antibody de-N-glycosylation was carried out by incubating the sample with PNGase F (New England Biolabs, Ipswich, MA) for 24 hrs at 37 OC (enzyme:substrate ratio ca ...
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bioRxiv - Plant Biology 2023Quote: ... The precipitates were eluted into 30μL 1×SDS loading buffer and detected using anti-Myc and anti-MBP antibody (1:5,000, New England Biolabs).
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bioRxiv - Plant Biology 2020Quote: ... The amplified products were gel purified (Monarch DNA Gel Extraction Kit, New England Biolabs) and incubated with 100 ng XbaI- and XhoI-digested pYL156 in a molar ratio of 1 vector ...
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bioRxiv - Plant Biology 2019Quote: Small RNA sequencing libraries were prepared using the NEBNext Small RNA kit (NEB, E7300L) according to manufacturer’s recommendations and sequenced by either the University of Missouri DNA Core or the University of Arizona Genetics Core with either an Illumina HiSeq2500 or NextSeq500 ...
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bioRxiv - Bioengineering 2021Quote: ... The PCR product was gel extracted with the Monarch DNA Gel Extraction Kit (NEB). 20,000 HEK-293FT cells were plated into 96 well plates ...
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bioRxiv - Microbiology 2020Quote: DNA amplification was performed using the Q5 DNA polymerase kit (New England Biolabs, USA) and the corresponding primers (Table S3 ...
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bioRxiv - Microbiology 2020Quote: ... was made by mutagenesis PCR using the site-directed mutagenesis kit (New England Biolabs). Additional mutants of this intermediate vector were generated through site-directed mutagenesis of the HHRz sequence ...
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bioRxiv - Molecular Biology 2020Quote: ... fragmented mRNA using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB), by omitting the RNA fragmentation step and following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... libraries were generated using the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645) and sequenced on an Ilumina NextSeq 550 with 75 bp single-end reads ...
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bioRxiv - Developmental Biology 2021Quote: ... The first strand cDNA was synthetized using ProtoScript II kit (New England Biolabs, USA) from 1 μg of total RNA or 1.5 ng of mRNA with Random Primer Mix (2 and 0.5 μl ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were column-purified (Monarch PCR & DNA Clean-up Kit, New England BioLabs) and submitted for Sanger sequencing (Genewiz ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was rRNA depleted using NEBNext rRNA Depletion Kit (New England BioLabs, E6310L). 1st strand cDNA was generated using Maxima H minus First Strand cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Libraries were prepared using the NEBNext Immune Sequencing Kit for Human (New England Biolabs) according to the manufacturer’s instructions without modifications ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted using the Monarch Genomic DNA purification kit (New England Biolabs). 400ng genomic DNA was used to construct whole-genome bisulfite libraries ...
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bioRxiv - Genetics 2021Quote: ... and sequencing libraries were generated using the NEBNext Ultra DNA Library prep kit (NEB) and NEB multiplex oligos (NEB ...
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bioRxiv - Genetics 2021Quote: ... 2 µL of Taq DNA polymerase (LongAmp Taq DNA Polymerase kit, New England Biolabs), 7.5 µL of dNTPs (dNTP set ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) (zebrafish ...
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bioRxiv - Microbiology 2020Quote: ... All reactions were performed using the LongAmp® Taq PCR Kit (New England Biolabs). Amplification conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... Different KRAS mutants were generated using Q5® Site-Directed Mutagenesis Kit (NEB, E0554S). The successful clones were confirmed using DNA sequencing.
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bioRxiv - Molecular Biology 2021Quote: ... and subjected to DNA library preparation via the NEBNext Ultra II kit (NEB, E7645L). Libraries were pooled at equimolar concentrations and sequenced via an Illumina NextSeq 500 system using paired end 150 cycles ...
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bioRxiv - Microbiology 2021Quote: ... Mutants of the bGSDM genes were constructed using Q5 Site-directed Mutagenesis kit (NEB). The Runella bGSDM ...
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bioRxiv - Developmental Biology 2020Quote: ... Library concentration was determined by NEBNext Library Quant Kit for Illumina (New England Biolabs). Paired end sequencing (76 cycles ...
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bioRxiv - Microbiology 2022Quote: ... Connecting the DNA segments was performed using the NEBuilder HiFi DNA Assembly Kit (NEB) or Ligation high Ver.2 (TOYOBO) ...
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bioRxiv - Molecular Biology 2022Quote: ... the FLuc Control Template from the HiScribe T7 high yield RNA synthesis kit (NEB) was in vitro transcribed and purified as described the linearized pTXB1 vector.
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bioRxiv - Neuroscience 2022Quote: ... were produced by using in vitro transcription HiScribe™ T7 ARCA Kit (NEB, E2060S) following the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... the PCR product was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Bromodomain mutants were generated using the Q5 site-directed mutagenesis kit (New England Biolabs) and each mutation was confirmed through DNA sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... sequencing using NEBNext UltraII DNA library Prep Kit for Illumina (New England BioLabs, Inc) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and the digestion product was purified using Monarch PCR & DNA Cleanup Kit (NEB, T1030S). Following the preparation of both the backbone vector and insert ...