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Citations for New England Biolabs :
2151 - 2200 of 10000+ citations for Human Nucleoredoxin like protein 1 NXNL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Transcripts were purified with a Monarch RNA purification kit (NEB) and quantified with a Qubit™ RNA broad-range assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Using ProtoScript First Strand cDNA Synthesis Kit (New England BioLabs), RNA is denatured and cDNA was synthesized ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Luna Universal One-Step RT-qPCR Kit (NEB), and then analyzed with QuantStudio Design & Analysis Software v.1.5.1 ...
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bioRxiv - Biochemistry 2024Quote: ... using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs), or by nicking mutagenesis32,60 ...
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bioRxiv - Microbiology 2023Quote: ... and Luna Universal qPCR Master Mix kit (New England Biolabs). Reactions were performed as technical replicates within each of three biological replicates ...
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bioRxiv - Microbiology 2024Quote: ... Isolation was performed using Monarch Plasmid Miniprep Kit (NEB, UK).
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bioRxiv - Developmental Biology 2024Quote: The PCR-based Q5 Site-Directed Mutagenesis Kit (NEB, #E0554S) was used to perform truncation (constructs L-P ...
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bioRxiv - Microbiology 2024Quote: ... coli strain carrying the plasmid (Monarch Mini-Prep kit, NEB) and transformed it into electrocompetent K ...
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bioRxiv - Genetics 2023Quote: ... and concentration determined using NEBNext Lib Quant Kit (E7630, NEB). Libraries were sequenced with an Illumina NextSeq550 with 42bp per read (total of 84bp ...
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bioRxiv - Bioengineering 2024Quote: ... The LunaScript Primer-Free RT Master Mix Kit (NEB E3025S) was used to generate cDNA per manufacturer protocol ...
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bioRxiv - Biophysics 2024Quote: ... using the NEBuilder HiFi DNA Assembly kit (New England Biolabs); by strictly following the protocols recommended by the manufacturers ...
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bioRxiv - Biophysics 2024Quote: ... and R88 using the Q5 site-directed mutagenesis kit (NEB). Giant spheroplasts were generated as described previously (Martinac et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNA was purified using the MonarchR RNA Cleanup Kit (NEB). The integrity of the purified RNA was determined by running RNA in a 1% agarose gel ...
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bioRxiv - Molecular Biology 2024Quote: ... NEBuilder® HiFi DNA Assembly Kit (catalog no. E2621L, NEB) was used to clone the sequence of interest into plasmid backbone.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). We verified the sequences of plasmid DNAs purified from transformed E ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were isolated using Monarch Plasmid Miniprep kit from NEB and linearized using restriction enzyme by NEB as per manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... The HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) was used to transcribe the template DNA per the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... purified using the Monarch® DNA gel extraction kit (NEB) and subjected to Sanger Sequencing for ORF sequence-verification prior to codon-optimization at Genscript and subcloning ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to manufacturer’s instructions using the following kits: NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina® (New England BioLabs) and pooled in equimolar quantities ...
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bioRxiv - Molecular Biology 2022Quote: ... Ribosomal RNA was depleted of using the NEBNext® rRNA Depletion Kit and cDNA libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). RNA was barcoded using the NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 x concentrated Phusion HF buffer and 1 U Phusion DNA Polymerase (New England Biolabs). The PCR reaction was performed as follows ...
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bioRxiv - Biochemistry 2021Quote: ... 1 ng/μl of template DNA and 1 U of Phusion polymerase (New England Biolabs). The mutant PCR fragments were then cloned into pSpurMS2 (a gift from Dr ...
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bioRxiv - Genetics 2023Quote: ... PCR products were visualized on 1% agarose gels with a 1 kb plus ladder (NEB).
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bioRxiv - Biochemistry 2023Quote: ... with 1 μg RNA and 1 unit RNase Inhibitor (New England Biolabs Inc., Massachusetts, USA), 5 μg methyltransferase or no enzyme ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM EDTA] plus 1 µL 20 µg/µL lambda phage DNA (NEB, cat # N3011S) and was separated on a 6% polyacrylamide / 0.5x TBE / 1.5 mm gel for 2 hr at 110 V in a Novex electrophoresis system ...
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bioRxiv - Biochemistry 2023Quote: ... Desalted peptides were dissolved in 1 ml 1 × CutSmart buffer (pH 8, New England BioLabs) and incubated with 50 units of alkaline phosphatase (calf intestinal ...
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bioRxiv - Microbiology 2024Quote: ... the ExoI reaction mixture containing 1 x ExoI buffer and 1 U/μL ExoI (NEB) enzyme was prepared ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× NEBuffer 3.1 (New England Biolabs) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× NEBuffer 3.1 (New England Biolabs) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg μl-1 bovine serum albumin (New England Biolabs® Inc., Ipswich, MA, USA), 0.2 μM of each primer and 2 μl of template DNA ...
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bioRxiv - Molecular Biology 2020Quote: Cleavage of the MBP tag from NlGr7 was completed according to manual instructions of the pMAL protein fusion and purification system (NEB, Inc, USA). The tag (MBP ...
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bioRxiv - Molecular Biology 2020Quote: ... the desired sequences pertaining to the truncated forms of the N protein were subcloned from the synthetic gene using polymerase chain reaction (Phusion polymerase, New England Biolabs, Hertfordshire, UK). All genes were cloned into the modified pet28 vector and gene sequences were confirmed by Sanger Sequencing.
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bioRxiv - Bioengineering 2020Quote: ... protease mutant S219V [24] was inserted at the 3’ end of gene encoding maltose binding protein (MBP) in pMAL-c5E vector (New England Biolabs, MA, USA) to construct pMAL-TEV vector ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μl of the gRNA mixture was mixed with Cas9-NLS protein (final concentration of 5 μM. New England Biolabs, Ipswich, USA), 2M KCl (final concentration of 300mM) ...
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bioRxiv - Microbiology 2021Quote: ... 39 μl of each lysate was combined with 5 μl of 10X NEB buffer for Protein MetalloPhosphatases (New England Biolabs, Ipswich, MA), 5 μl of 10 mM MnCl2 and 1-2 μl (400-800 units ...
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bioRxiv - Molecular Biology 2022Quote: ... deglycosylation treatment (Dglyco) consisted of sample incubation for 3 hours at 37°C with 20 nL of Protein Deglycosylation Mix II (NEB, cat. P6044S) per μL of sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins (except from cells expressing eGFP and cytoplasmically tagged EGFR) were further treated with 250 U of PNGase F (NEB, Cat# P0704) for 1 hour at 37 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or four (white shavenbaby experimental) sgRNA targeting the first or second exon (100 ng/μl) and CAS9 protein (EnGen Spy Cas9 NLS from NEB, Catalog # M0646M). The distribution of the major trichome height for the white control was normal (Shapiro-Wilk normality test W = 0.9637 ...
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bioRxiv - Microbiology 2024Quote: ... 39 μl of each benzonase-treated lysate was combined with 5 μl of 10X NEB buffer for Protein MetalloPhosphatases (New England Biolabs, Ipswich, MA), 5 μl of 10 mM MnCl2 and 1 μl (400 units ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA was isolated with Oligo-dT Beads from NEB and RNAseq libraries were constructed with the Directional Kit from NEB. Sequencing was performed at IPS2 Paris ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was set up using a commercial kit (PURExpress NEB) according to user guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... The NEBNext ultra II RNA library prep kit (New England Biolabs) was used to generate poly(A)+ libraries ...
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bioRxiv - Microbiology 2020Quote: ... the overhanging ends were blunted using a blunting enzyme kit (NEB) and an A-tail was added with Taq DNA polymerase (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... using the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Allelic replacement of the hlyA open reading frame by this constructs ...
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bioRxiv - Developmental Biology 2021Quote: ... Mutagenic primers and the Q5 site-directed mutagenesis kit (NEB E0554S) were used to generate the BD-Chinmonon-stop and the CG11180/Chigno-sim* mutant ...
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bioRxiv - Cell Biology 2019Quote: ... The NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) was used to generate TruSeq-barcoded RNA-Seq libraries ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs) and the online tool NEBasechanger to design the mutagenesis primers ...
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bioRxiv - Microbiology 2020Quote: ... The ChiZ overexpression construct was engineered using the NEBuilder kit (NEB), according to the manufacturer’s instructions with primers PA311 + PA312 and LW043 + LW044 and the pBRplac-lacI derivative ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA fragment ends were repaired using Quick Blunting™ kit (NEB), purified with QIAquick column (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ChIP-seq libraries were made following the NEBnext UltraII kit (NEB) and associated protocol ...