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Citations for New England Biolabs :
2101 - 2150 of 10000+ citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... anchored primer was used to create a complementary strand to the TdT extended products using 15 units of Klenow Fragment (3′→5′ exo-) (NEB) in 1× NEB2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Base editing sgRNA directed against the SF3B1 K700 locus was designed using BE-Designer28 and cloned into pLKO.5 Puro-2A-GFP between BsmBI (New England Biolabs) cut sites ...
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bioRxiv - Cancer Biology 2024Quote: ... the hU6-pegRNA2-polyT cassette was amplified from the pU6-pegRNA-gg-acceptor backbone and ligated into ngRNA1 pLKO.5 Puro-2A-RFP between EcoRI and XhoI (New England Biolabs) to create pLKO.5-K700E-ng1+pg2-Puro-2A-RFP ...
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bioRxiv - Biochemistry 2024Quote: ... and tRNAGln with 5 nt-long 5’ leader and same 24 nt-long 3’ trailer as tRNATyr (5–tRNAGln–24) – were transcribed in reactions containing 1x T7 RNA polymerase reaction buffer (NEB), 0.001% (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Adapter sequences were then removed by restriction digest (PCR reaction product, 1X rCutSmart NEB Buffer, 5 U EcoRV-HF (NEB)) at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Adapter sequences were then removed by restriction digest (PCR reaction product, 1X rCutSmart NEB Buffer, 5 U EcoRV-HF (NEB)) at 37°C for 1 hour ...
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bioRxiv - Genomics 2024Quote: ... dATP (3x 1.5 µL of 10 mM solutions) and 8 µL of 5 U/µl Klenow fragment of DNA polymerase I (New England Biolabs) and a 30-minute incubation at 37°C with rotation ...
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bioRxiv - Genomics 2024Quote: ... 120 µL 10x T4 DNA ligase buffer and 5 µL of 400 U/µL T4 DNA ligase (New England Biolabs) was added ...
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bioRxiv - Genetics 2024Quote: The ligation reaction was performed in a 1:5 plasmid to insert copy number ratio using T4 DNA ligase (NEB) at 16°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... according to manufacturer’s instructions using 100 nM RNA-specific reverse transcription primer followed by RNase H digest with 5 µL RNase H (NEB) at 37 °C for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... The radioactive probe was prepared from 40 pmoles of D072 primer (Table 1) and labeled at the 5’ end with 10 units of T4 polynucleotide kinase (New England Biolabs) and [γ-32P]-ATP (150 μCi) ...
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bioRxiv - Biochemistry 2024Quote: ... GoldenGate reactions were performed with 5 U of restriction enzyme and 200 U of T4 ligase in T4 ligase buffer (NEB) also containing 0.1 mg/ml BSA (NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each of the synthesized DNA fragments was amplified by PCR and inserted at the 5’ of the GFP gene in the pPha-NR vector (NovoPro Bioscience) using NEBuilder HiFi DNA Assembly (New England BioLabs). Five μg of the pPha-NR constructs were introduced into P ...
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bioRxiv - Plant Biology 2020Quote: ... respectively by Phusion® High-Fidelity PCR Kit (NEB) (Harashima & Schnittger ...
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bioRxiv - Plant Biology 2019Quote: ... and the Luna One-Step RT-qPCR Kit (NEB) in accordance with the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... After purified with Monarch RNA Cleanup Kit (NEB Biolabs), RNAs were polyadenylated with E ...
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bioRxiv - Cancer Biology 2021Quote: ... After purified with Monarch RNA Cleanup Kit (NEB Biolabs), RNAs were polyadenylated with E ...
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bioRxiv - Molecular Biology 2020Quote: ... and qPCR analysis by NEB Next Library Quant Kit for Illumina (New England Biolabs). All sequencing was performed using an Illumina MiSeq (Illumina ...
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bioRxiv - Cell Biology 2020Quote: Libraries were prepared using Next Ultra II kits (NEB) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Using the NEBuilder HiFi DNA assembly cloning kit (NEB), EBOV VP30 was inserted into a pLenti6.3 vector (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: PCR fragments were assembled using Gibson assembly kit (NEB) and verified by Sanger sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... and reverse transcribed using Lunascript kit (New England Biolabs). qPCR was performed on a Light Cycler 480 (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... purified with the Monarch PCR & DNA Cleanup Kit (NEB) and ligated with T4 DNA ligase (NEB) ...
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bioRxiv - Developmental Biology 2021Quote: ... Using Q5® Site-Directed Mutagenesis Kit (NEB, E0554), we generated mutations in each of the two most highly conserved Rbpj binding sites (m1 and m2 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... NEBNext® Microbiome DNA Enrichment Kit (New England BioLabs) was used for increasing the proportion of bacterial DNA (via the procedure of selective binding and disposing of methylated host DNA) ...
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bioRxiv - Developmental Biology 2021Quote: ... we used the Q5 Site Directed Mutagenesis kit (NEB) to generate the sqhT20A mutation in the pattB -sqhWT construct using oligos H334 and H335 (See Table 2) ...
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bioRxiv - Genomics 2020Quote: ... We used the NEBuilder HiFi DNA Assembly Kit (NEB) to assemble the purified inserts and the backbone of MPRA plasmid (previously digested with EcoRV) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and the Unique Dual Indexing Kit (New England Biolabs), with reagent volumes reduced to one-fifteenth of recommended volumes ...
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bioRxiv - Molecular Biology 2021Quote: ... with the NEBNext rRNA Depletion Kit (NEB, cat# E6310L) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, #E5520) was used according to user’s manual ...
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bioRxiv - Genomics 2020Quote: ... and NEB Quick DNA ligase kit (NEB cat. E6056S) by incubation at 16°C overnight without a heated lid ...
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bioRxiv - Molecular Biology 2021Quote: ... and ProtoScript II First Strand cDNA Synthesis Kit (NEB) with Oligo-dT primers ...
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bioRxiv - Microbiology 2019Quote: ... or Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Base triplets encoding S423 and T424 were changed to code for alanine or for aspartate and glutamate resulting in kaiC36803-AA and kaiC36803-DE genes ...
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Weak Membrane Interactions Allow Rheb to Activate mTORC1 Signaling Without Major Lysosome EnrichmentbioRxiv - Cell Biology 2019Quote: ... The Q5 Site-Directed Mutagenesis Kit (New England Biolabs) was used to generate GFP-RhebΔCAAX ...
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bioRxiv - Microbiology 2019Quote: ... and ligated using the Quick Ligation Kit (NEB, USA) to generate the targeting plasmid ...
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bioRxiv - Genomics 2021Quote: ... NEBNext multiplex oligos for Illumina Kit (New England Biolabs) was used in adapter ligation and PCR amplification steps ...
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bioRxiv - Genomics 2020Quote: ... using the NEBNext Ultra DNA Library kit (NEB #E7370) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... ProtoScript-II first-strand cDNA synthesis kit (NEB, USA), Brilliant-III ultrafast SYBR Green qPCR master mix (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... or a PURExpress bacterial IVT kit (New England Biolabs), following the manufacturer’s recommended protocol similar to that described previously45 ...
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bioRxiv - Microbiology 2021Quote: ... The WarmStart LAMP Kit (Cat# E1700S, New England BioLabs) was utilized using SARS-CoV-2 specific primers[43] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs) ...
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bioRxiv - Bioengineering 2021Quote: ... using NEBuilder HiFi DNA Assembly Cloning Kit from NEB. Synthetic DNA was purchased from IDT as gBlocks (Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2021Quote: A Q5 site-directed mutagenesis kit (NEB, Ipswitch, MA) was used to generate pcDNA3.1-p300-FLAG from the plasmid pcDNA3.1-p300-His6 obtained from Addgene (Catalog number 23252 ...
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bioRxiv - Biophysics 2021Quote: ... and generated using NEBNext DNA Library Prep Kit (NEB). Samples were indexed with unique barcodes using NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Neuroscience 2021Quote: ... we used the Q5 Site-Directed Mutagenesis Kit (NEB) and generated site-specific mutations in met cDNA ...
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bioRxiv - Neuroscience 2021Quote: Using NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520), human wild-type ...
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bioRxiv - Cancer Biology 2021Quote: ... a PCR-based Q5 site-directed mutagenesis kit (NEB) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... using the Ultra II FS Kit (New England Biolabs). Libraries were quantified using a BioAnalyzer (Agilent ...
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bioRxiv - Systems Biology 2021Quote: ... and then purified with the Monarch PCR kit (NEB). PCR amplicons were digested using MfeI-HF and SphI-HF (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... The NEBuilder HiFi DNA Assembly kit (New England Biolabs) was used to combine the two split EPG sections with N-Terminal HaloTag Plasmid PHTN (Promega ...
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bioRxiv - Systems Biology 2020Quote: ... the NEBNext Ultra II DNA Library prep kit (NEB) was used for libraries with size ranges from 200-400bp ...