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Citations for New England Biolabs :
2051 - 2100 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was isolated using the mRNA Isolation kit (NEB, S1550S). mRNA was fragmented using the NEBNext Magnesium RNA fragmentation module (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... Ribosomal RNA was removed using the rRNA Depletion kit (NEB) and multiplexed RNA-seq libraries were generated using the NEBNext Ultra Directional RNA library Prep Kit for Illumina (NEB) ...
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bioRxiv - Developmental Biology 2024Quote: ... using the Q5 site-directed mutagenesis kit (New England Biolabs). These substitutions did not alter protein sequences ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were extracted using Monarch Plasmid Miniprep kit (NEB) and precipitated by cold ethanol ...
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bioRxiv - Neuroscience 2024Quote: ... with NEB Next Library Quant Kit for Illumina (NEB, E7630L) and KAPA Library Quantification Kit (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... and purified using Monarch® RNA Cleanup Kit (NEB, T2040). An RNA/DNA mix was obtained by retrotranscribing 200pmol of RNA + 200pmol of the Reserve primer (5’-TTGTCGCACGTTCGGTGTCG-3’ ...
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bioRxiv - Microbiology 2024Quote: Experiments with PURExpress in vitro protein synthesis kit (NEB, E6800) were performed as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and purified with the Monarch RNA clean-up kit (NEB). cDNA was prepared from 1 μg of RNA ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using NEBuilder HiFi DNA assembly kit (NEB). DNA was quantified on a Nanodrop Lite or Qubit ...
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bioRxiv - Neuroscience 2024Quote: ... and hybridized with NEBNext rRNA Depletion Kit v2 (NEB E7400) to substantially diminish rRNA from the samples ...
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bioRxiv - Molecular Biology 2024Quote: ... The NebNext® UltraTM II DNA library prep kit (NEB) for Illumina was used for sequencing library preparation following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to manufacturer’s instructions using the following kits: NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina® (New England BioLabs) and pooled in equimolar quantities ...
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bioRxiv - Molecular Biology 2022Quote: ... Ribosomal RNA was depleted of using the NEBNext® rRNA Depletion Kit and cDNA libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). RNA was barcoded using the NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... or a region in the N-terminal part (for TGDs) were PCR amplified (see Table S4 for primer sequences) from 3D7 or IT4 gDNA (Monarch gDNA Purification Kit, NEB or QIAGEN DNA extraction kit) and cloned into pSLI (Birnbaum et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction incubated with Exonuclease I (2U, add 1μl of a 10-fold dilution of NEB Exonuclease I) for 15 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: 3’ dephosphorylation was performed by incubating fragments with 10 U/uL T4 Polynucleotide Kinase (New England Biolabs M0201S) in the supplied buffer (NEB B0201S ...
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bioRxiv - Developmental Biology 2020Quote: ... 37.5 μl of 0.4 mM biotin-14-dATP and 10 μl of 5 U/ μl Klenow (NEB, M0210L) were added and mixed by pipetting ...
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bioRxiv - Genomics 2020Quote: ... 1 mM ATP and 10 mM DTT and with 1 μl (2 U/μl) of RNase H (NEB), 1 μl (3 U/μl ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysis was cleared by centrifugation at 16,100g for 10 min and 0.65 ml supernatant was mixed with 50μl Protein G beads (NEB, #37478S) pre-conjugated to U1-70K antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... RNA was denatured again and pyrophosphates were removed from the 5’-end by 10 U RppH (M0356S, NEB) in 1× NEB buffer 2 (10 mM Tris-HCl pH 7.9 ...
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bioRxiv - Molecular Biology 2019Quote: ... About 2-3 μg of source DNA plasmid (<10 kb) was added with NEBuffer 3.1 (NEB, cat# B7203S), DEPC ddH2O in a volume of 30 μl and incubated at 37 °C for >2 hour (h) ...
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bioRxiv - Genomics 2019Quote: ... 50 µL of NEB Buffer 3.1 and 20 µL of 10 U/µL DpnII restriction enzyme (NEB, R0147) were then added ...
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bioRxiv - Systems Biology 2019Quote: ... then flow in 2mL of wash buffer (10% ethylene carbonate in TBS with 2mM Vanadyl Ribonucleoside Complex (NEB)) ...
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bioRxiv - Microbiology 2020Quote: ... followed by 10 cycles of annealing at 54°C for 30 s) using Taq polymerase (New England Biolabs) according to the manufacturer’s instructions and the primers 5-CTTGACATCTCTGAGGCAAC-3 and 5-ATGCAGGGGCAAAGTCATTAG-3 ...
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bioRxiv - Microbiology 2021Quote: Each peptide was tested for its ability to be cleaved by recombinant furin (10 U/mL; NEB; P8077) in a buffer of 100 mM HEPES ...
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bioRxiv - Synthetic Biology 2021Quote: ... Each reaction consisted of 10 μl of Luna Universal Probe qPCR Master Mix (New England Biolabs, Ipswich, MA), 5 μl of standard or sample ...
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bioRxiv - Microbiology 2021Quote: ... 100ng of vector was digested for 1 hour at 37°C with 10 U of BamHI-HF (NEB) to create a single double-stranded cut or 10 U Nb.BtsI (NEB ...
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bioRxiv - Immunology 2021Quote: Purified gDNA samples were diluted 10-fold and in parallel into Cutsmart-buffered BamHI-HF (New England Biolabs) restriction digestions in the presence of 0.1% Pluronic F-68 (50μL final volume ...
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bioRxiv - Genomics 2020Quote: ... 0.2 μL 10 mM dNTPs (Kapa Biosystems, Woburn, MA, 0.1 μL Q5 Polymerase (New England Biolabs, Ipswich, MA), 0.5 μL forward primer (10 μM) ...
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bioRxiv - Biophysics 2021Quote: ... The medium was then exchanged for 150 μL 10 nM SNAP-Dy549 (SNAP-Surface 549, New England Biolabs) in complete medium and incubated for a further 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 50 μl of DNase I mixture (25 μl of 10× DNase I buffer, 18 μl of NEB DNase I ...
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bioRxiv - Cell Biology 2019Quote: ... cells were collected and incubated in fresh SM containing 10 µM SNAP-Cell Block (New England Biolabs S9106), for 30 min at 25°C ...
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bioRxiv - Biochemistry 2019Quote: ... the fluidic cell was incubated with 10 mg/ml bovine serum albumin (BSA, New England Biolabs, Ipswich, USA) to prevent non-specific surface binding ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 µg of total protein (9 µl of lysate) were treated with Endo Hf (New England Biolabs) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... UltraScript 2.0 (PB30.31-10, lot #PB130614-01-5, PCR Biosystems, Wayne PA) and WarmStart RTx (M0380L, lot #0061705, NEB) by each manufacturer’s protocol with minor modifications ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1.5 μL of 10 mM dNTPs mix,1.5 U of NEB Taq DNA polymerase (New England Biolabs, USA) and 10 pmol of each primer and 10-200 ng of DNA template ...
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bioRxiv - Evolutionary Biology 2020Quote: ... approximately 500 ng of total RNA was used for a 10 ul cDNA synthesis with ProtoScript II (NEB). 15 uM of the appropriate strand-specific primer (IDT ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products were treated with 10 units (0.5 μL) of DpnI restriction endonuclease (New England Biolabs Ltd., #R0176), incubated at 37°C for 30 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pooled duplex DNA (20 μL) was phosphorylated using T4 polynucleotide kinase (10 units; M0201S, New England Biolabs) in 10X T4 DNA ligase buffer (2 μL ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei from pools of at least 10 distal or proximal limbs were digested with DpnII (New England Biolabs) and ligated with T4 DNA ligase HC (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... A 10% fraction of the chromatin lysate was digested using 200 U of Taq I (New England Biolabs) at 60°C for 7 h ...
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bioRxiv - Molecular Biology 2021Quote: ... for 12 h at 4 °C with 10 μg of Factor Xa protease (New England Biolabs, Hitchin, UK) per 1 g of mitochondria ...
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bioRxiv - Microbiology 2020Quote: ... at final volume 20 μL with 10 μL of Luna Universal qPCR Master Mix (New England Biolabs, Germany). The concentration of the primers varied from 0.2-0.5 μM (for further details regarding genes ...
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bioRxiv - Molecular Biology 2020Quote: ... and crosslinks were reversed with 10% SDS and 0.6 mg/ml Proteinase K (New England Biolabs, Ipswich, MA) overnight with shaking at 65 °C ...
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bioRxiv - Genetics 2019Quote: ... and 2.0 units of the CspCI enzyme ((N)10-11CAA(N)5GTGG(N)12-13) (New England Biolabs). The library preparation was carried out according to the protocol proposed by Osorio-Guarín et al ...
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bioRxiv - Neuroscience 2021Quote: ... Phosphatase treated samples were enzymatically treated for 3 n at 37°C shaking (1.25 μl 10 μM MnCl2, 1.25 μl 20X NEB buffer ...
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bioRxiv - Genomics 2022Quote: ... 13.1 μL Murine RNAse inhibitor, 5.25 μL Tartrazine (10 mg/mL, Carl Roth) and 13.2 μL T4 DNA ligase (New England Biolabs). 4 μL of the master mix were added to the Ligation Barcoding Plate #1 or #2 (see above ...
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bioRxiv - Immunology 2022Quote: ... The nuclease-digested RNA was then dephosphorylated by adding 10 units/µg of Quick CIP enzyme (NEB, M0525) and incubating at 37 °C for 90 min (Kellner et al. ...
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bioRxiv - Microbiology 2022Quote: ... 1-20 μg of the Env protein was mixed with 1 μl of Glycoprotein Denaturing Buffer (NEB, 10×) and H2O (if necessary ...
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bioRxiv - Molecular Biology 2023Quote: ... and decapped with 10 U of T4 PNK +/− 40 nmol of ATP and 5 U of RppH (NEB). After each step ...