Labshake search
Citations for New England Biolabs :
2051 - 2100 of 10000+ citations for Mouse Acrosomal Protein SP 10 ACRV1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... The library of each sample was prepared separately with the NEBNext® Ultra (tm) II FS DNA Library Preparation Kit and the Unique Dual Indexing kit (New England Biolabs), with reagent volumes reduced to one-fifteenth of that advised by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... was then gel purified using the Qiagen MinElute Gel Extraction Kit (28604) and subjected to Gibson assembly via the New England Biosciences HiFi DNA Assembly Cloning Kit (NEB: E5520S) to insert the amplified region into the hSyn-eGFP vector following its linearization with BamHI (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were qualified on Agilent 2100 Bioanalyzer using High Sensitivity DNA kit and quantified by qPCR using NEBNext Library Quant kit for Illumina (NEB, E7630). Equal molarity of each library were pooled and subjected to sequencing on Illumina HiSeq 4000 and NovaSeq 6000 platforms at the McGill University and Génome Québec Innovation Centre to generate 50 bp paired-end reads.
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bioRxiv - Pathology 2021Quote: ... The kit used for library construction was the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Total RNA extracted from the samples was subject to mRNA enrichment using poly-T oligo-attached magnetic beads ...
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bioRxiv - Genomics 2021Quote: ... We individually indexed samples to be pooled within the same sequencing lane during the PCR enrichment step using NEBNext® Multiplex Oligos for Illumina® (single-indexed with E7335 and E7500 kits, or dual-indexed with E6440 kit, New England Biolabs). We performed in-solution hybridization and target enrichment using our 12,049 tiled RNA probes ...
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bioRxiv - Genetics 2021Quote: ... Vector components were purified using the Monarch® DNA Gel Extraction Kit or the Monarch® PCR & DNA Cleanup Kit (NEB) and assemblies were performed using NEBuilder® HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: RNA-seq libraries for RIP-TRIBE and polysome were prepared in triplicates from 150 ng of total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with the NEBnext rRNA depletion Kit (NEB #E6310L). cDNA libraries were sequenced on an Illumina NovaSeq6000 using S-prime or S1 flow cells with paired-end 150-bp reads ...
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bioRxiv - Biochemistry 2022Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s instructions (New England Biolabs, MA, USA). Sequencing experiments were performed with NextSeq 500 (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s instructions (New England Biolabs, MA, USA). Sequencing experiments were performed using NextSeq 500 (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... Library quality was checked by Agilent BioAnalyzer 2100 using the High Sensitivity DNA kit (Part number 5067-4626) and libraries were quantified using the Library Quant Kit for Illumina (NEB #7630). Libraries were then sequenced using a NextSeq500 platform (75-base-pair (bp ...
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bioRxiv - Molecular Biology 2022Quote: ... Library quality was checked by Agilent BioAnalyzer 2100 using the DNA 1000 kit (Part number 5067-1504) and libraries were quantified using the Library Quant Kit for Illumina (NEB #7630). Libraries were then sequenced using a NextSeq500 platform (75-base-pair (bp ...
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bioRxiv - Biochemistry 2023Quote: ... loci were amplified using the NGSgo®-MX6-1 kit (GenDx) and multiplexed sequencing libraries were prepared using the Ultra™ II FS DNA Library Prep Kit for Illumina (NEB). Libraries were sequenced on a NovaSeq 6000 system (Illumina ...
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bioRxiv - Immunology 2023Quote: ... These amplicons were used as templates for in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap® Reagent AG kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription into cDNA was performed using a cDNA synthesis kit (ProtoScript First Strand cDNA Synthesis Kit, New England BioLabs, Ipswich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s protocols (New England Biolabs, MA, USA), as described previously58 ...
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bioRxiv - Molecular Biology 2023Quote: Libraries were qualified on Agilent 2100 Bioanalyzer using High Sensitivity DNA Kit and quantified by qPCR using NEBNext Library Quant Kit for Illumina (NEB, E7630). Equal molarity of each library was pooled and subjected to sequencing on Illumina NovaSeq 6000 platform at the McGill University and Génome Québec Innovation Centre to generate 100 bp paired-end reads.
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bioRxiv - Biophysics 2023Quote: ... Vector DNA was linearized with NheI or MluI and cRNA synthesized in vitro using the SP6 or T7 AmpliCap Max High Yield Message Maker Kit (Cellscript, USA) or HiScribe® T7 ARCA mRNA Kit (New England Biolabs) and stored at −20°C (for frequent use ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized with RevertAid First Strand cDNA Synthesis Kit (ThermoScientific) reverse transcription kit and PCR was carried out using OneTaq® DNA Polymerase reagents (NEB) using conditions previously detailed and oligonucleotides described in Suppl ...
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bioRxiv - Cell Biology 2024Quote: ... samples were run on a 1% agarose gel and areas corresponding to the expected size were excised and purified from the gel using a gel extraction kit (Monarch DNA Gel Extraction Kit, NEB, USA). This PCR product was used for a second round of amplification followed by gel purification ...
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bioRxiv - Developmental Biology 2020Quote: A Fragment Analyzer™ Automated CE System (Analysis Kit: cat.: DNF-474-0500) was used to assess the fragment distributions and a Library Quant Kit for Illumina (NEB, cat.: E7630L) was used to measure molar concentrations ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg/ml glycogen) and DNA purification was carried out with the PCR cleanup kit (NEB, Monarch PCR & DNA Cleanup Kit, T1030). About 10ng of the purified CUT&RUN DNA was used for preparation of multiplexed libraries with the NEB Ultra II DNA Library Prep Kit per manufacturer’s instruction (NEB #E7103) ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-seq libraries were synthesized using an NEB Next Ultra RNA Library Prep Kit for Illumina and an NEB Next Adaptor for Illumina Kit (New England Biolabs, MA, USA). Paired-end sequencing was performed using the HiSeqXten (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA sequencing libraries were prepared from 100 ng of input RNA using the non-directional kit NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB, #E7775) with the NEBNext Poly(A ...
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bioRxiv - Microbiology 2022Quote: Site directed mutagenesis was carried out using Q5® Site-Directed Mutagenesis Kit using manufacturer’s protocol (source of kit, NEB or else) using primers carrying the altered codon were designed using the online tool OligoCalc ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were prepared according to the instructions of the Kit “NEBNext Ultra Directional RNA Library Prep kit for Illumina” (E7760L, New England Biolabs, Ipswich, MA), following the protocol “Poly(A ...
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bioRxiv - Immunology 2023Quote: The total RNA of macrophage groups was isolated with a Nucleo-Spin RNA kit (Macharey Nagel, Germany) or Monarch Total RNA Miniprep Kit (New England Biolabs, Massachusetts, ABD). The purity and quantity of RNA were evaluated by a Nanodrop spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated using a genomic DNA extraction kit (Monarch Genomic DNA Purification Kit, New England Biolabs, Frankfurt am Main, Germany) and the PCR was optimized to yield a single amplicon ...
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bioRxiv - Microbiology 2023Quote: ... Stranded RNA-seq libraries were constructed using NEBNext poly(A) mRNA isolation kit along with the SWIFT RNA Library Kit (NEB, MA, USA) according to the established protocols at Laboratory for Molecular Biology and Cytometry Research (LMBCR ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was purified using the QIAquick PCR purification kit and it was prepared for Illumina sequencing using the NEBNext Ultra II A Library Prep Kit (New England Biolabs; Cat# E7645L).
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... 10 ng of genomic DNA was amplified using LongAmp Taq 2X Master Mix (New England BioLabs) and the provided barcoded 16S primers ...
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bioRxiv - Cell Biology 2019Quote: ... We incubated cells in complete medium containing 10 μM of SNAP-Cell Block (New England Biolabs) to block all available pre-existing SNAP-tag (Quench) ...
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bioRxiv - Genetics 2021Quote: ... we digested 10 μg of each vector with AvrII (#ER1561; Thermo Fischer) and XcmI (#R0533; NEB) and performed a gel-purification ...
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bioRxiv - Genomics 2020Quote: ... 1 μL of 10 mM dATP and 15 units of Klenow 3’ → 5’ exo- (NEB M0212L) was added to blunted ends and incubated at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The 5’-end of dephosphorylated mRNAs were then labeled using 10 units of T4 PNK (NEB) and 0.5 µl [γ-32P] ATP (6000 Ci/mmol ...
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bioRxiv - Molecular Biology 2020Quote: 10 μg of genomic DNA was digested overnight with 20 units of AluI and MboI (NEB) and then precipitated with isopropanol ...
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bioRxiv - Genomics 2022Quote: ... 10 μl 2X Quick T4 ligase buffer and 2 μl Quick T4 DNA ligase (NEB, M2200L) were added to the reaction and incubate at 37 °C for overnight ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification (8-9 cycles) was performed with 10 μL Fusion HF Buffer (New England Biolabs), 3.125 μL 10uM TruSeq Primer 1 ...
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bioRxiv - Genomics 2020Quote: The polyadenylation product was mixed with 0.5 μL 10 mM dNTPs (New England Biolabs, Cat. # N0447L), 1 μL reverse transcription primers (25 ng/μL ...
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bioRxiv - Genetics 2019Quote: ... The reannealed amplicon was then digested with 10 units of T7 Endonuclease I (New England Biolabs) at 37 °C for 90min ...
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bioRxiv - Neuroscience 2019Quote: ... and then mounted using 10 μl ProLong Gold Antifade Reagent containing DAPI (9071S, New England Biolabs). The slides were imaged when the mounting medium was fully cured >12 h.
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bioRxiv - Molecular Biology 2019Quote: ... 10 mM CaCl2 and 1x PIC) containing 2U/μl of micrococcal nuclease (New England BioLabs, M0247S) and incubating on ice for 20 min and then 37°C for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μl reaction mixture composed by 10 μl of Phusion High-Fidelity Master Mix 2X (NEB), 2 μl of Betaine 5M (Sigma-Aldrich) ...
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bioRxiv - Physiology 2021Quote: ... RNA 3’ end dephosphorylation reaction consisted of T4 PNK (20 U/10 μL sample, NEB M0201S), SuperaseIn in 1X T4 PNK buffer without ATP for 60 min at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 µl of PCR product was then mixed with 1.5 µl 10X NEBuffer 2 (B7002S, NEB) and 1.5 µl of Nuclease-free water ...
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bioRxiv - Genomics 2021Quote: ... DNA samples were labeled with 10 μL of a cocktail of repair enzymes (PreCR mix, NEB), fragmented ...
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bioRxiv - Genomics 2020Quote: ... using 10 units each of MluCI and NlaIII restriction enzymes (New England Biolabs, Beverly MA, USA), NEB CutSmart buffer ...
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bioRxiv - Bioengineering 2020Quote: ... The post-transcription reaction mixture was incubated with 10 units of DNase I (New England Biolabs) at 37°C for 1 hour to remove the gblock template then purified using a RNA spin column (Zymo Research) ...
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bioRxiv - Biochemistry 2021Quote: ... RCA products were debranched using 10 units/ µg DNA of T7 Endonuclease I (New England Biolabs) for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of crude mitochondria were mixed with 1/10 volume gel loading dye (7025, NEB) and subjected to Semi-Denaturing Detergent Agarose Gel Electrophoresis (SDD-AGE) ...
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bioRxiv - Microbiology 2020Quote: ... We incubated 10 pmol gRNA with 5 pmol SauCas9 (New England Biolabs [NEB], Ipswich, MA, USA) at 25 °C for 10 min ...