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Citations for New England Biolabs :
2001 - 2050 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared with NEBNext Multiplex small RNA library prep Kit (NEB#7300) using the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared with NEBNext Ultra II DNA library prep kit (E7645S, NEB), size-selected for 150 bp insert ...
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bioRxiv - Molecular Biology 2023Quote: ... The genomic DNA was extracted using Monarch Genomic DNA purification kit (NEB T3010). Two rounds of amplification for gRNA from the genomic DNA were performed ...
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bioRxiv - Molecular Biology 2023Quote: ... the genomic DNA was extracted using Monarch Genomic DNA purification kit (NEB T3010) followed by PCR amplification of the gRNA cassette using primers compatible with Illumina sequencing and NEB Q5 Hot Start High-Fidelity master mix (NEB M0494 ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were prepared with the NEBNext RNA Sample Preparation kit (New England BioLabs) according to the manufacturer’s protocol ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... were inserted into the digested vector using a HiFi DNA assembly kit (NEB). Alternatively ...
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bioRxiv - Genomics 2023Quote: ... Library concentration was quantified using NEBNext Library Quant Kit for Illumina (NEB E7630). Pooled libraries were sequenced using NextSeq500 in 75 bp paired-end mode ...
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bioRxiv - Genomics 2023Quote: ... Library concentration was quantified using NEBNext Library Quant Kit for Illumina (NEB E7630). Pooled libraries were sequenced using NextSeq500 in 75 bp paired-end mode ...
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bioRxiv - Bioengineering 2022Quote: ... and assembled into the pTlpA39-Wasabi vector (Addgene #86116)[46] using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, GmbH). The mCherry gene was inserted downstream of the PtlpA promoter by replacing the mWasabi reporter gene ...
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bioRxiv - Biochemistry 2023Quote: ... The points mutations were inserted using the Q5 mutagenesis kit (New England Biolabs) and specific primers ...
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bioRxiv - Biochemistry 2023Quote: ... was produced by QuikChange site-directed mutagenesis (NEB, Phusion High-Fidelity PCR Kit) followed by KLD (Kinase ...
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bioRxiv - Biochemistry 2023Quote: ... was generated using site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB E0554S) of the SARS-CoV-2 Positive Control (N gene ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was first circularized by a T4 RNA Ligase 1 Kit (M0204. NEB) and then purified by TRIzol reagent followed by isopropanol precipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... After quantification by qPCR using the NEBnext library quant kit for Illumina (NEB), pooled libraries were sequenced on an Illumina HiSeq 2500 platform using a 50 bp single read on a high output standard v4 flow cell with a 10–20% PhiX spike-in ...
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bioRxiv - Cancer Biology 2022Quote: ... DHFR control plasmid from PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used as backbone for subsequent ligations ...
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bioRxiv - Genomics 2022Quote: ... The resulting fragments were purified using the Monarch PCR & DNA Cleanup Kit (NEB). Libraries were constructed using Illumina Nextera Dual Indexing ...
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bioRxiv - Genomics 2022Quote: ... the original ORI was excised using a Site Directed Mutagenesis kit (NEB E0554S). The vector was transformed (NEB C3040H ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries was constructed using NEBnext Ultra II DNA library prep kit (NEB) following the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and mutations were introduced using the Q5 Site-Directed Mutagenesis Kit (NEB # E0554S) or were synthesized by Genscript ...
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bioRxiv - Molecular Biology 2023Quote: ... dsRNA was generated by in vitro transcription using the HiScribe T7 kit (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Secondary PCRs were purified with the Monarch PCR and DNA Cleanup Kit (NEB), ligated into the pCR2.1-TOPO TA cloning vector (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... double strand cDNA was synthesized using NEBNext mRNA second strand synthesis kit (NEB), double strand DNA was washed with AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Plant Biology 2023Quote: ... Library was prepared using the NEBNext Ultra Directional RNA Library Prep Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... rRNA depletion was conducted using NEBNext rRNA Depletion Kit (Bacteria, New England Biolabs) according to the manufacturer’s recommended protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was purified by Monarch® RNA Cleanup Kit (New England Biolabs). Binding of ribosomes to leaderless mRNA was tested by assembling 10-500 fmol of 70S ribosomes with 10 fmol of NAD/ ppp-leaderless mRNA in translation buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... and the NEBNext Ultra II directional RNA library Prep kit (NEB, Cat#E7785S) for Illumina were used for index library prep following NEB #E7760S/L ...
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bioRxiv - Bioengineering 2023Quote: The Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs, USA) was used ...
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bioRxiv - Bioengineering 2023Quote: ... and the linearized plasmid was re-ligated with a Quick Ligase Kit (NEB) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearized DNAs were purified using Monarch DNA cleanup kit (NEB, United States) and the capped RNA transcripts were synthesized using the SP6 mMessage mMachine kit (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing libraries were prepared using NEB Next Ultra II kit (New England Biolabs). The resulting DNA quantity and size distribution was assessed by Qubit instrument (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Removal of this codon by site-directed mutagenesis (Q5 kit, New England Biolabs) yielded the WIL-V-Chis ...
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bioRxiv - Plant Biology 2023Quote: ... followed by purification with the Monarch RNA Clean Up Kit (NEB, Frankfurt, Germany). gDNA removal was assessed by PCR on chloroplast DNA followed by agarose gel electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... ribosome footprints were purified using the Monarch RNA cleanup kit (NEB cat# T2030) following the modified protocol for small RNA.
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bioRxiv - Biochemistry 2023Quote: ... The deletion was done by Gibson assembly using the Gibson Assembly Kit (NEB). The restriction enzyme ClaI (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... and plasmid DNA extracted using the Monarch Plasmid Miniprep Kit (New England Biolabs), and Sanger sequencing used to confirm correct insertion of coding sequences at the Ramaciotti Centre for Genomics (UNSW Sydney) ...
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bioRxiv - Biochemistry 2023Quote: Cas12a mutants were cloned using Q5 polymerase and KLD kit (New England Biolabs) and purified the same way as WT AsCas12a.
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bioRxiv - Synthetic Biology 2023Quote: ... and plasmids were extracted using the Monarch Plasmid Miniprep Kit (New England Biolabs). Both pEndo and pTarget were transformed into E ...
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bioRxiv - Molecular Biology 2023Quote: ... The libraries were then purified using the Monarch PCR & DNA Cleanup Kit (NEB), using a 5:1 ratio of Binding Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the plasmid above was modified using the Q5 Site-Directed Mutagenesis Kit (NEB) along with the following primers ...
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bioRxiv - Genomics 2023Quote: ... The NEB UltraII kit (New England Biolabs, Ipswich, MA, USA, cat. no. E7646A) was used for end-repair and A-tailing ...
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bioRxiv - Synthetic Biology 2023Quote: ... for large rearrangements and Q5® Site-Directed Mutagenesis Kit (New England BioLabs) for small modifications i ...
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bioRxiv - Genetics 2023Quote: ... and libraries prepared using NEB Ultra II DNA Library Prep Kit (NEB, #E7645S) and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 4 ...
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bioRxiv - Plant Biology 2023Quote: ... Reactions were purified using a Monarch PCR & DNA Cleanup Kit (New England Biolabs) and sent to Source BioScience ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: ... RNA was extracted using TRIzol and column purification (NEB Monarch RNA cleanup kit), and samples were then individually diluted in NEBuffer 2 (NEB ...
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bioRxiv - Genomics 2023Quote: ... rRNA depletion was performed using the NEBNext® rRNA Depletion Kit (Bacteria) (NEB) and the sequencing libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The resulting DNA fragments were cloned using the Gibson Assembly Cloning Kit (NEB) into plasmid backbone pUN4 to obtain pUN-OXA48WT ...
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bioRxiv - Immunology 2023Quote: ... and NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB; Ipswich, USA). RNA-Seq was then performed at a depth of 50M reads/sample on an Illumina NovaSeq 6000 S4 instrument of 100 base-pair long paired-end sequence reads (excluding adapter/index sequences) ...
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bioRxiv - Cell Biology 2023Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB 7760) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Plasmid DNA was purified using the Monarch Plasmid DNA Miniprep Kit (NEB, #T1010), and subjected to diagnostic restriction digest and subsequent gel electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... Double-stranded RNA was purified using Monarch RNA Cleanup Kit (50 µg; NEB) and eluted in 30 μl of nuclease-free water ...