Labshake search
Citations for New England Biolabs :
2001 - 2050 of 5199 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... equal amount of DNA (∼2 ng) was used as an input for NEB Ultra II DNA library prep kits (NEB #E7645). Number of cycles for amplification of adapter ligated libraries were estimated by the qPCR before final amplification to avoid any bias arising due to PCR amplification and indexing (NEB #E7350) ...
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bioRxiv - Biochemistry 2022Quote: ... the sample was diluted to 50 μL with ammonium bicarbonate buffer and incubated at 37 °C with 2 μL of PNGase F (New England Biolabs P0705S) diluted 1:100 in ammonium bicarbonate for an additional 7 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Dephosphorylation of CRL4B took place in a 100 μl reaction mixture containing 86 μl of CRL4B protein (at 0.8 mg/ml) with 2 μl λ-phosphatase (λ-PP) in the presence of 0.1 mM MnCl2 and 1x PMP reaction buffer (NEB). Untagged-CRL4 complexes (4A ...
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bioRxiv - Cell Biology 2022Quote: ... the sequence encoding amino acids 1-490 was amplified with NdeI and EcoRI overhangs and inserted into a modified backbone based on pSNAP-tag(T7)2 (NEB #N9181S) before a SNAPf-EGFP-6His tag (Budaitis et al. ...
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bioRxiv - Cell Biology 2022Quote: ... An IFT144 construct lacking the N-terminal β-propeller domain (residues 2–349 inclusive; IFT144ΔNFLAG) was made using the Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Genetics 2022Quote: ... 500 ng of purified PCR products (D2500, Gel Extraction Kit, OMEGA, USA) were denatured and reannealed in NEB buffer 2 (M0302S, NEB, USA): 95 ℃ ...
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bioRxiv - Microbiology 2022Quote: RNA-free PXO99A genomic DNAs (0.2 μg) were used to construct the DNA libraries using a NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The transformation vector pICU2:Cas9-dsRED containing the Cas9 gene from Streptococcus pyogenes expressed under the promoter of the Arabidopsis Incurvata 2 gene (ICU2, At5g67100) was combined with the gRNA combinations by Gibson assembly (NEB, USA). For RPB1 ...
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bioRxiv - Plant Biology 2022Quote: ... including the stop codon and (2) the CDS and native promoter region 1024 bp upstream using Phusion High Fidelity DNA polymerase (NEB, USA) and TA-ligated into the entry vector pCR8/GW/TOPO (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were next resuspended in 1X Thermo Pol Buffer and treated with 2 µL RNA 5’ Pyrophosphohydrolase (New England Biolabs M0356) at 37°C for 1 h to promote decapping of 5’ RNA ends ...
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bioRxiv - Biochemistry 2023Quote: ... The mutant and wild-type target RNA were subsequently amplified using either the NEB Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB E3019), Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB M3019 ...
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bioRxiv - Cell Biology 2022Quote: ... The mutant fragments were amplified by high-fidelity DNA polymerase 2 × Phanta Max Master Mix followed by DpnI (New England BioLabs; R0176S) digestion in 37°C for 1 hour to eliminate the templates ...
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bioRxiv - Genetics 2023Quote: ... PCR products were analyzed on 2% Agarose gels with 0.5 ng/L Ethidium bromide using a 1kb Plus DNA Ladder (New England BioLabs Cat # N3200S) for size reference ...
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bioRxiv - Genetics 2022Quote: ... and 2 μg of DNA was digested with 50 units of NlaIII and 5 μL CutSmart® Buffer (NEB, cat #R0125L), in a total volume of 50 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... All mRNAs were transcribed at 30°C for 2 hrs using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) and were co-transcriptionally capped (8:1 cap analog to GTP for ∼90% capping efficiency ...
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bioRxiv - Biochemistry 2023Quote: ... was used to linearize plasmid (10 µg) by incubating at 37 °C for a minimum of 2 hours in 1x CutSmart buffer (NEB, B7204S). Linearized plasmid was purified by extraction with an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were created using 1000ng of total RNA with the NEBNext Ultra 2 Directional RNA Library Kit following the Poly(A) mRNA Magnetic Isolation module (NEB #E7490). Libraries were sent to Novogene for Illumina Hi-Seq ...
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bioRxiv - Developmental Biology 2023Quote: ... The 2-kpb cbln12 promoter (Dohaku et al., 2019) and lTl-Kaede-pAS were subcloned to pT2ALR-Dest by NEBuilder (NEB, USA). To generate Tg(5xUAS-hsp70l:HA-skor2-P2A-mCherry ...
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bioRxiv - Plant Biology 2023Quote: ... and PEP444c were amplified using a cDNA template obtained from 2-week-old barley shoots and roots and Q5® High-Fidelity DNA Polymerase (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The cDNAs were diluted 1:10 and 2 μl of each used for subsequent PCR reactions with one unit of Taq polymerase (New England Biolabs, MA), 200uM dNTPs (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... 250 ng of RNA samples were digested at 37°C for 2 hours with Nucleoside Digestion Mix (New England Biolabs, M069S). Digested RNA samples were diluted to 100 µl with double-distilled water and filtered through 0.22 µm Millex Syringe Filters ...
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bioRxiv - Evolutionary Biology 2024Quote: ... ddRAD library preparation included an initial digestion of 300 ng of DNA in a 34 μL reaction (2 hours at 37 °C; 10 U each of SbfI and MspI, New England Biolabs Inc.). Standard Illumina adapters were ligated using 60 cycles of digestion at 37 °C (2 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... and Gas5 shRNAs (shRNA # 1= G5C1 and shRNA # 2= G5C2) were cloned by replacing the existing Luciferase shRNA (shRLuc) under a U6 promoter using BamHI (NEB #R0136L) and EcoRI restriction sites (Table 3) ...
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bioRxiv - Biochemistry 2024Quote: ... and ligated to pre-adenylated linkers (NI-810 to NI-815) containing 5 nt sample barcodes unique for each sample using truncated T4 RNA ligase 2 (K227Q) (NEB; M0351L). Ligated fragments were separated from free linkers on a 15% polyacrylamide TBE-Urea gel and then pooled and purified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... Final nuclear lysates were resuspended using 100 µl of resuspension solution (1x PBS, 2% BSA, 0.2U/µl RNase inhibitor; New England Biolabs, cat# M0314S). Hoechst staining was performed to assess the quality of isolated nuclei based on their morphology ...
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bioRxiv - Genomics 2024Quote: ... The reaction was purified using the Zymo DNA Clean & Concentrator kit and PCR-amplified with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L) and primers as defined in Corces et al ...
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bioRxiv - Zoology 2024Quote: ... The annealed dsRNAs (2.5 µg) were checked on 1.5% agarose gel by running them together with 2 µL of dsRNA ladder (NEB# N0363S, Germany) (Fig ...
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bioRxiv - Cell Biology 2024Quote: RNA encoding indicated HSP90B1 nascent chains were prepared from PCR amplified and purified DNA template containing a T7 promoter and carried out at 37°C for 2 hours using the HiScribe® T7 High Yield RNA Synthesis Kit (New England Biolabs) and purified using the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... with the genotype fhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10--Tet)2 [dcm] R(zgb-210::Tn10--Tet) endA1 Δ(mcrC-mrr)114::IS10(New Engalad Bioloabs, NEB) was used in this study ...
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bioRxiv - Microbiology 2024Quote: SARS-CoV-2 N (nucleocapsid) protein qPCR system (IDT, Iowa City, IA) and RSV N qRT-PCR (Luna, NEB, Ipswich, MA) were detected under one-step qRT-PCR on a QuantStudio 3 (Applied Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... plasmid pYT354 and around 2000 base pairs (2 kb) upstream and downstream the region of interest were PCR-amplified by Q5® High-Fidelity DNA Polymerase (NEB) using primers with 20 overlapping base pairs designed with Benchling ...
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bioRxiv - Genomics 2024Quote: ... 1.2 µl of linker oligonucleotide (Integrated DNA Technologies) were mixed with 2 µl 10x 5’DNA adenylation buffer (New England Biolabs, E2610L), 0.095 mM ATP ...
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bioRxiv - Synthetic Biology 2024Quote: Transcription templates for expressing gRNA variants and SARS-CoV-2 or CMV input RNA fragments were generated by PCR (Phusion high-fidelity PCR kit, NEB #E0553) of the gBlock or Ultramer template that included a T7 promoter and the gRNA or input RNA coding sequence ...
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bioRxiv - Molecular Biology 2024Quote: ... and for Figure 2 using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). Input DNA was also used as a control ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of 10 μM uniquely barcoded universal i5 and i7 primers (Supplementary Table 2) as well as 25 μL NEBNext Ultra II Q5 2x PCR Master mix (NEB, M0544L) were added to the 21 μL DNA and a short PCR was performed (72°C for 5 min ...
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bioRxiv - Microbiology 2024Quote: Tax1bp1 was amplified by PCR from murine cDNA using the primers named Tax1bp1 fwd and rev (Table 2) and inserted by ligation-independent cloning (NEBuilder Builder HiFi DNA Assembly Cloning Kit, NEB #E5520S) into pENTR1A no ccdB (w48-1 ...
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bioRxiv - Genomics 2024Quote: ... First we re-amplified cDNA by combining 20 ng of the remaining cDNA from channel 2 of each sample together with 50 µl of NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs, M0541S), 2 µl of 12 µM PCR Primer1 (5’-CTACACGACGCTCTTCCGATCT-3’) ...
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bioRxiv - Molecular Biology 2024Quote: ... the pHAGE lentiviral plasmid encoding SARS-CoV-2 N-LgBIT and N-SmBIT were generated by NEBuilder HiFi DNA Assembly (New England Biolabs E2621S). Oligonucleotide primers were obtained from Azenta Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... and COPG1 cDNA was PCR amplified using gene specific primers (Supplementary Table 2) and Q5® High-Fidelity DNA Polymerase (New England Biolabs). Gel purified (Macherey-Nagel ...
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bioRxiv - Genetics 2024Quote: ... We amplified the pgRNA cassette from the genomic DNA by performing three rounds of PCR using 500 ng of genomic DNA for each reaction and NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541S). We deep sequenced the purified libraries with paired-end sequencing ...
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bioRxiv - Genetics 2024Quote: ... we amplified the pgRNA cassette from the cloned plasmid library by three rounds of PCR with NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541S). The DNA sequences of oligos used for pgRNA library cloning are listed in Supplementary Table 3 ...
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bioRxiv - Developmental Biology 2024Quote: The coding sequence for the HaloTag was extracted by 2-step PCR amplification with Phusion High-Fidelity DNA Polymerase (NEB, M0530) from the pFC27A HaloTag CMV-neo Flexi vector (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... were then ligated to pre-annealed Nano-tRNAseq 5’ and 3’ RNA adapters at room temperature for 2 hours with 20% PEG PEG 8000 (NEB, B10048) using recombinant E ...
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bioRxiv - Cell Biology 2022Quote: ... annealed and phosphorylated sgRNA (1:10) and T4 Ligase reaction buffer (1:10, #B0202S, New England Biolabs) with 10 mM 10x Adenosine 5’-Triphosphate (#P0756S ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µl freshly made 1 M NH4HCO3 and calf intestinal alkaline phosphatase (1 U, New England Biolabs) were added ...
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bioRxiv - Molecular Biology 2020Quote: ... Proximity ligation was done under the following conditions: 1 unit/µl RNA ligase 1 (New England Biolabs), 1x RNA ligase buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA pH 8.0 (Roth) and 1:100 Proteinase K (New England Biolabs 20 mg/ml)) in a 2 ml Eppendorf tube for at least 24 hours at RT in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µl of 1 mM BG-(PEG)12-biotin (New England Biolabs; PEG linker available on request) was added to 200 µl of axonemes ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of each pFRT-TO-CLIP-UPF1 mutant vector was digested with 1 µL SpeI (NEB) and 1 µL HindIII-HF (NEB ...
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bioRxiv - Microbiology 2023Quote: ... A 1 ml aliquot of each phage was treated with 1 µl DNase (New England Biolabs, USA) at 37 ⁰C for 40 minutes ...