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Citations for New England Biolabs :
151 - 200 of 1985 citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... and 0.12 µM SiR-SNAP-Tag ligand (NEB, S9102S) for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal anti-SNAP-tag (New England BioLabs, P9310S), mouse monoclonal anti-HSP90 (BD Transduction Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... anti-SNAP-tag antibody (New England Biolabs, Ref. P9310S), anti-clathrin heavy chain antibody (BD Bioscience ...
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bioRxiv - Neuroscience 2020Quote: ... The DNA amplified fragments (Table S1) were digested with XhoI and NheI restriction enzymes (NEB). Expression of the resulting genes led to fusion proteins containing a His6 tag and a TEV Niα protease cleavage site ...
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bioRxiv - Biochemistry 2021Quote: RNA substrates (listed in Table S1) were either 5’ labelled by T4 polynucleotide kinase (NEB) and 5’ 32P-γ −ATP ...
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bioRxiv - Plant Biology 2021Quote: ... restriction enzyme analysis (Supplemental Table S1) was performed following the manufacturer’s recommendations (NEB, Bethesda, USA). The final PCR products were analyzed via 1.5% (m/v ...
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bioRxiv - Genomics 2022Quote: ... we spike in 2.5 µL of 3M U/mL T7 DNA ligase (NEB M0318L) and continue incubating for 1 hour at 25C ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragment encoding SNAP-tag was amplified from the pSNAP-tag (m) vector (a gift from New England Biolabs & Ana Egana, Addgene #101135), and then inserted into the pDisplay vector (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Fusions to the SNAP-tag (New England Biolabs, Beverly, MA) were expressed from plasmid pAGT-Xpress ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... SNAP-tag® ligand Blue 430 (New England Biolabs, S9109S) was applied for 30 min at a final concentration of 2 µM ...
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bioRxiv - Biophysics 2021Quote: ... The His6-MBP tag was recaptured using amylose resin (NEB). The flow-through was loaded onto a Q-HP anion exchange column (Cytiva ...
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bioRxiv - Systems Biology 2022Quote: ... The SNAP tag insert was derived from pSNAPf (NEB, N9183S) and the FRB-Fis1 insert was derived from pC4-RhE-FRB-Fis1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... SNAP-tag comes from pSNAPf-ADRβ2 control plasmid (NEB, #N9184). SNAP-CB2-TwinStrep-1D4 ...
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bioRxiv - Molecular Biology 2023Quote: ... for Strep-tag II pull-downs and Amylose resin (NEB) for MBP pull-downs) ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... the MPB tag was cleaved overnight using Factor Xa (NEB) in dialysis buffer and loaded again on an MBP-Trap HP column ...
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bioRxiv - Cell Biology 2024Quote: ... pSNAPf vector containing SNAP-tag was purchased from NEB (N9183S) and optimised to Drosophila codon suing Genewiz (USA ...
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bioRxiv - Biochemistry 2021Quote: The Bam35 genomic DNA was used to amplify the gene 2 flanked by KpnI and BamHI sites by PCR with B35SSB_FW_KpnI and B35sSB_RV_BamHI primers (Table S1) and Vent DNA Polymerase (New England Biolabs). The digested PCR product was cloned into a pET-52b(+ ...
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bioRxiv - Physiology 2022Quote: ... sense and anti-sense oligo DNAs (IDT) (Table S1) were phosphorylation using T4 Polynucleotide Kinase (NEB) at 37 °C for 30 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... we use Western Blot to detect spike protein with/without treatment of PNGase F (NEB). 100 ng particles were incubated with Glycoprotein Denaturing Buffer at 98°C for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... plates were immunostained using a monoclonal antibody against SARS-CoV2 nucleoprotein (Creative-Biolabs; NP1C7C7) at a dilution of 1:1000 followed by 1:5000 anti-mouse IgG monoclonal antibody and was developed using KPL TrueBlue peroxidase substrate for 10 minutes (Seracare ...
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bioRxiv - Microbiology 2022Quote: ... pCAGGS.SARS-CoV-2_SΔ19_fpl_mNG2(11)_opt was generated through gibson assembly (NEBuilder, New England Biolabs) of a pCAGGS vector backbone cleaved using EcoRV-HF and HindIII-HF (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: pCAGGS.SARS-CoV-2_SΔ19_fpl_mNG2(11)_opt was generated using NEBuilder DNA assembly (New England Biolabs) of a pCAGGS vector backbone cleaved using EcoRV-HF and HindIII-HF (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... and tRNA-Gly-CCC (Table S1) were radioactively labeled with gamma-32P-ATP using T4 PNK (NEB). Pre-hybridization and hybridization were performed in OligoHyb Buffer (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
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bioRxiv - Plant Biology 2021Quote: ... digested alongside pYL156 with restriction enzymes as listed in Supplementary Table S1 (enzymes from New England Biolabs), and ligated with Promega T4 DNA ligase (Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
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bioRxiv - Biophysics 2022Quote: ... and degradation tags were amplified by PCR with Q5 polymerase (NEB), gel extracted (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... The SNAP-tag sequence was amplified using pSNAPf vector (#N9183, NEB) and inserted into the AscI and KpnI sites after UBQ10 promoter in the UBQ10pro-p4p1r entry vector ...
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bioRxiv - Systems Biology 2022Quote: ... BCKDK was purified by its MBP tag on amylose resin (NEB).
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bioRxiv - Cell Biology 2022Quote: ... the GST-6xHis-tag was removed by TEV protease (#P8112S, NEB) and dArc1 and dArc2 proteins were further purified by size exclusion chromatography using Superdex S200 16/600 (GE Healthcare Life Sciences).
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bioRxiv - Cell Biology 2022Quote: ... EGFP was replaced by a ybbR-tag using Q5 mutagenesis (NEB #). For kif5b ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Original SNAP tag and Nanoluciferase sequences were from NEB (Hitchen UK) and Promega (Southampton UK ...
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bioRxiv - Biophysics 2022Quote: ... Three are commercially available orthogonal self-labeling approaches: SNAP-tag (NEB), CLIP-tag (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... and CLIP-tag ligands (CLIP surface 647 - BC 647 [NEB, S9234S]) at final concentrations of 1 μM in 0.3% PBT ...
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bioRxiv - Biophysics 2019Quote: ... 27P-XhoI-A and XbaI-A oligonucleotides (Table S1) were biotin or digoxigenin tailed using Terminal Transferase (NEB) and either BIO-dUTP or DIG-dUTP ...
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bioRxiv - Plant Biology 2020Quote: ... Table S1) were used to amplify GmSALT3 cDNA with Phusion® High-Fidelity DNA Polymerase (New England Biolabs) using 35 cycles (98 °C 30 s ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... using the primers outlined in Table S1 and Q5 Hot-start high fidelity polymerase (New England Biolabs M0494S). PCR products were extracted from standard agarose gels using the QIAquick Gel Extraction Kit (Qiagen 28704 ...
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bioRxiv - Developmental Biology 2023Quote: Plasmids (otxG:Tet-ON 3G; TRE3Gs:Cas9; TRE3Gs:Kaede; U6:sg2; Table S1) were built by Gibson cloning (NEB, Cat# E2611) following standard protocols ...
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bioRxiv - Biochemistry 2020Quote: ... Tag introduction and mutagenesis was achieved through Gibson assembly (New England Biolabs) to generate SMC1-His ...
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bioRxiv - Microbiology 2022Quote: ... Affinity tags were introduced by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) into the expression vectors encoding Rae1 and Nup98 using primers in Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... Then the His-tag was cleaved by incubation with TEV protease (NEB) for 18 h at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... and the His6-MBP tag was subsequently recaptured using amylose resin (NEB). The flow-through was loaded onto a Q-HP anion exchange column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... and subsequently the 6xHIS tag was cleaved using the TEV Protease (NEB) by adding TEV enzyme assuming all protein isolate was 6xHIS tagged based on the activity of the TEV protease ...
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bioRxiv - Biochemistry 2023Quote: ... and SNAP tags were stained with SNAP-Cell® 505-Star (NEB). HaloTag® Ligands TMRDirect were added to medium to a final concentration of 50nM and incubated overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... The 3XFLAG tag was replaced by Q5® site-directed mutagenesis (NEB) with either a Myc-tag or Strep-tag®II ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins with MBP-tag were loaded onto amylose resin (New England Biolabs) after elution from Ni-NTA ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs). Regularly ...
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bioRxiv - Biophysics 2020Quote: The transient transfection of HEK293 cells was performed67 using the TransFectin Lipid Reagent (Bio-Rad) (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... HEK293 libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat. #E7770) or NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (#E7760) ...
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bioRxiv - Immunology 2021Quote: ... The kRSV-DB1-QUAD plasmid and spike insert were digested with the enzymes AatII and SalI (NEB) and ligated with T4 DNA ligase (NEB ...