Labshake search
Citations for New England Biolabs :
151 - 200 of 1314 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: MukF Flag-tagged fragments were expressed from pET 21 plasmids in C3013I cells (NEB).The Flag-tagged MukE and MuF were at the C-terminus ...
-
bioRxiv - Cell Biology 2021Quote: ... MBP-tagged fragments were purified by gravity flow through amylose resin (New England Biolabs) and step elution in maltose ...
-
bioRxiv - Biochemistry 2023Quote: ... containing a His6-tagged Ulp1 gene was transformed into T7 Express competent cells (NEB) and grown in LB medium supplemented with ampicillin (100 μg/mL ...
-
bioRxiv - Biochemistry 2024Quote: ... His6-tagged GATE16 was generated via Q5 site-directed mutagenesis PCR (New England Biolabs), replacing the GST tag with the sequence MHHHHHHGS ...
-
bioRxiv - Bioengineering 2024Quote: ... the Myc-tagged MAGT sequences were amplified by PCR with Q5 DNA polymerase (NEB) and primers listed in Supplementary Data 3 ...
-
bioRxiv - Plant Biology 2021Quote: ... The glutathione S-transferase (GST) tag was cleaved using Factor Xa Protease (New England Biolabs, Ipswich, MA, USA) in accordance with the manufacturer’s instruction.
-
bioRxiv - Biophysics 2023Quote: ... assembled with GST tag from pGEX6P plasmid and inserted into pACEBac1 vector via HIFI DNA assembly kit (NEB). The full length KIF5B in pACEBac1 was replaced with a C-terminal TEV-Twin-Strep-tag via HIFI DNA assembly kit (NEB) ...
-
bioRxiv - Plant Biology 2023Quote: ... AtPAXXL228E fused to hexa-histidine followed by a GST tag (pGAT3-atpaxxL228E) was expressed in BL21(DE3) (NEB) and purified using a 5 ml GSTrap HP column (Cytiva) ...
-
bioRxiv - Biophysics 2024Quote: Full-length human tau protein with a 6x His-tag was cloned into the pRK172 vector and expressed in LEMO21 cells (NEB #C2528J). Cells were cultured in TB media at 37 °C until the OD600 reached 0.6 ...
-
bioRxiv - Immunology 2021Quote: Purified recombinant CV3-25 IgG was mixed with LysC (NEB) at a ratio of 1μg LysC per 10mg of IgG and incubated at 37°C for 18h with nutation ...
-
bioRxiv - Developmental Biology 2020Quote: ... Kinase assays were performed using recombinant murine c-Abl (NEB). The amounts of GST-fusion protein to add to the reaction were calibrated using western blots of the purification products with mouse anti-NICD (1:1000 ...
-
bioRxiv - Cell Biology 2022Quote: ... PCR-amplified recombinant DNA was treated with DpnI (NEB Biolabs) for 3 h ...
-
bioRxiv - Cell Biology 2022Quote: ... PCR-amplified recombinant DNA was treated with DpnI (NEB Biolabs) for 3 h ...
-
bioRxiv - Biophysics 2021Quote: ... Amplified fragments were cloned into pET (His6) and pGEX (GST) based vectors using Gibson cloning method (NEB builder, E5520S). Plasmids used in this study is listed in Table S1.
-
bioRxiv - Biochemistry 2020Quote: pGEX6p2-GST-GβL was cloned from pRK5-HA-GβL into pGEX6p2-GST and was transformed into BL21 (DE3) cells (New England Biolabs) and purified using Glutathione Sepharose beads (45000139 ...
-
bioRxiv - Biochemistry 2021Quote: C-terminally (His)6-tagged LukE was expressed in BL21 (DE3) Escherichia coli cells (NEB). Transformed cells were grown at 37 °C in Terrific broth supplemented with 100 μg/mL ampicillin to a density of OD600 = 0.6 ...
-
bioRxiv - Immunology 2020Quote: ... and dephosphorylated using recombinant shrimp alkaline phosphatase (New England BioLabs M0371L). Annealed and phosphorylated oligonucleotides were cloned into linearized and dephosphorylated BPK1520_puroR using T4 DNA Ligase (New England BioLabs M0202L ...
-
bioRxiv - Immunology 2021Quote: ... A similar dilution series of recombinant histone H3 (New England Biolabs) was prepared starting at 1 µg/ml protein ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 5 mM DTT with 1.5 µM recombinant H3 (New England Biolabs), vehicle or 500 nM recombinant enzyme ...
-
bioRxiv - Biochemistry 2024Quote: ... Protease Inhibitors) with recombinant PKA kinase (2500 U/mg, NEB-P600S) and 1 mM ATP overnight at 30°C and 250 rpm ...
-
bioRxiv - Biochemistry 2022Quote: ... Recombinant bacmids were created by transformation into DH10Bac competent cells (NEB), screening on XGAL plates ...
-
bioRxiv - Genetics 2021Quote: ... FAN1 variants were introduced in the pFASTBAC1-GST-FAN1-FLAG-His plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Confirmed by DNA sequencing ...
-
bioRxiv - Cell Biology 2021Quote: ... the protein mix was incubated for 1 h at 4° C in 150 µL pull-down buffer supplemented with 15 µL glutathione agarose resin for GST pull-downs or amylose resin (New England Biolabs) for MBP pull-downs ...
-
bioRxiv - Cell Biology 2021Quote: ... and pGEX-GST-p130593-790 R680A L682A were generated by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... and ligated to linearized vector pGex-6p-1 (GST) or pET28-SUMO (His) also cut with the same restriction enzymes using T4 DNA ligase (NEB).
-
bioRxiv - Biophysics 2023Quote: ... and cloned into Sal I-digested GST vector pGEX-6P-1 (Cytiva) by NEBuilder HiFi DNA Assembly cloning (New England BioLabs). To express the GST fusion protein ...
-
bioRxiv - Microbiology 2023Quote: Epitope-tagged constructs using the GST tag or the 3X FLAG tag were generated using the HiFi DNA Assembly cloning kit (NEB). MntS was cloned into pGEX-6-1 and the GST-MntS fusion was subcloned into pKT25c such that the resulting plasmid lacked the T25 region ...
-
bioRxiv - Developmental Biology 2023Quote: ... Supernatants were separated from lysates and incubated with affinity beads at 4°C overnight (His beads: Ni-NTA from QIAGEN; GST beads: glutathione-agarose beads from ROTH; MBP beads: amylose beads from NEB). Protein-binding beads were collected by centrifugation and washed several times with washing buffer (His washing buffer ...
-
bioRxiv - Plant Biology 2023Quote: AtXRCC4 fused to a hexa-histidine followed by a GST tag (pGAT3-atxrcc4) was expressed in BL21(DE3) cells (NEB), The proteins were purified using GST sepharose (Cytiva) ...
-
bioRxiv - Cell Biology 2022Quote: All overexpression constructs with the internally tagged actins were generated by Gibson assembly (New England Biolabs). Briefly ...
-
bioRxiv - Cell Biology 2021Quote: ... PCR confirmation of endogenously tagged ATR cell lines was performed using NEB Taq DNA polymerase (NEB) as per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... PCR was performed with Nextera-tagged primers under standard Phusion or Q5 Polymerase (New England Biolabs). PCR primers used in this study can be found in Supplementary Table 3 ...
-
bioRxiv - Biochemistry 2022Quote: ... the refolded 6×His-tagged precursors were treated with enterokinase (New England Biolabs, Ipswich, MA, USA) to remove their N-terminal 6×His-tag according to our previous procedure [3,18] and purified by HPLC using an analytical C18 reverse-phase column (Zorbax 300SB-C18 ...
-
bioRxiv - Biochemistry 2022Quote: ... was cloned into pGex6P-1 in-frame with an N-terminal 3C-cleavable GST tag using HiFi assembly (New England Biolabs, USA).
-
bioRxiv - Biophysics 2024Quote: ... containing an N-terminal GST-mCherry tag and a nuclear localization sequence (NLS) was transformed into T7 Express cells (enhanced BL21 derivative, NEB C2566I) and grown to an OD of 0.4-0.7 at 37 °C ...
-
bioRxiv - Synthetic Biology 2021Quote: The recombinant CBX8-CD was expressed in BL21 (DE3) (New England Biolabs) Escherichia coli cells ...
-
bioRxiv - Biophysics 2021Quote: ... Recombinant M-MuLV reverse transcriptase from the ProtoScript® II kit (NEB) was used to synthesize first strand cDNA from the annealed primer-MS2 RNA mix ...
-
bioRxiv - Biochemistry 2022Quote: The recombinant Lili-Mips were treated with PNGase F (New England Biolabs) to remove the N-linked oligosaccharides ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant vectors were generated using HiFi DNA Assembly Cloning Kit (NEB E5520). During cloning procedures ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant vectors were constructed using HiFi DNA Assembly Cloning Kit (NEB E5520). electroporation (device) ...
-
bioRxiv - Biochemistry 2024Quote: 15 μg of recombinant LyLAP was deglycosylated using PNGase F (NEB, P0704) in either native or denaturing conditions ...
-
bioRxiv - Molecular Biology 2024Quote: ... PCR amplification was performed using recombinant Q5 DNA polymerase (New England Biolabs) and primers listed in Table S1 ...
-
bioRxiv - Developmental Biology 2021Quote: ... and amplified/tagged in two steps using NEBnext High-Fidelity 2x PCR master mix (New England Biolabs). Amplified DNA was purified twice with 1.8 volumes of NucleoMag NGS Clean-up and Size Select beads (Macherey Nagel) ...
-
bioRxiv - Biophysics 2020Quote: mNG-tagged ADRβ2 and EGFR were generating by cloning into the pSNAPf-ADRβ2 backbone (New England Biolabs). mNG was amplified by PCR from mNG-C1 and placed between the EcoRI and SbfI sites of pSNAPf-ADRβ2 (replacing the SNAP tag ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 mM CaCl2) at room temperature in the presence of recombinant enteropeptidase (NEB) at 13 U enzyme per mg TMPRSS2 zymogen ...
-
bioRxiv - Genomics 2024Quote: ... 200 µg/mL molecular biology grade recombinant albumin (rAlbumin) (New England Biolabs B9200S), 0.8 U/µL RiboLock RNase inhibitor (Thermo Fisher Scientific EO0384) ...
-
bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2 and 2 U of recombinant Escherichia coli RNase HI (NEB) were added ...
-
bioRxiv - Immunology 2024Quote: ... 1 µl of the recombinants was transformed into NEBstable competent cells (NEB, USA). Bacterial cells were spread on agarose plates ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The tagged pore variant plasmids were constructed by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB), where the variant encapsulin genes were individually amplified by PCR from the untagged pore variant plasmids ...
-
bioRxiv - Cell Biology 2022Quote: ... FLAG-tagged GPI-anchored PROM1- EX1 was generated by the DNA assembly method (#E2621, NEB, Ipswich, MA, USA). The GPI- anchor signal sequence from pCAG:GPI-GFP (#32601 ...