Labshake search
Citations for New England Biolabs :
151 - 200 of 9806 citations for Rat Heat Shock Protein Beta 6 HSP20 HSPB6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were recovered in 1 ml NEB® 10-beta/Stable Outgrowth Medium (NEB, B9035) at 37 °C for 1 hour ...
-
bioRxiv - Immunology 2020Quote: ... transferred to 42°C and digested by immediate addition of 2ul of beta-agaraseI (NEB) per 300ul of melted gel and incubation at 42°C for 1h ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... transformations that involved the CRE library were performed using electroporation (NEB 10-beta electrocompetent E.coli), in all other cloning steps chemically competent E ...
-
bioRxiv - Biochemistry 2024Quote: ... Purified products were again transformed into either NEB 10-beta (New England Biolabs, Ipswich, MA) or SIG10 (Sigma-Aldrich ...
-
bioRxiv - Synthetic Biology 2023Quote: ... according to manufacturer’s protocols and transforming chemically competent NEB 10-beta cells (NEB CN# C3019), where colony PCR and Sanger sequencing were again used to determine if the clone was correct.
-
bioRxiv - Molecular Biology 2024Quote: ... The cosmid-I95 plasmid was amplified in a NEB 10-beta (New England Biolabs, C3019H), and the DNA was purified using a QIAfilter Plasmid Midi Kit (Qiagen ...
-
bioRxiv - Molecular Biology 2019Quote: ... Heat mediated antigen retrieval pretreatment using 10 mM Sodium Citrate buffer (Vectors Biolabs) were used ...
-
bioRxiv - Molecular Biology 2024Quote: ... 6 and 4 μL of 6× Gel Loading Dye (B7025S, New England Biolabs) and 1.5 and 1 μL of 2.5 mg/mL EtBr were added ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
-
bioRxiv - Neuroscience 2021Quote: ... The polymerase chain reaction (PCR) was performed on a genomic rat DNA template using a Taq PCR Kit (New England Biolabs), and the subsequent PCR product was purified using a Qiagen PCR Purification Kit (Life Technologies ...
-
bioRxiv - Genomics 2021Quote: ... or by ribodepletion with NEBNext Human/Mouse/Rat rRNA Depletion kit followed by library construction using the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Illumina 2×151 paired end reads were generated either on the HiSeq 4000 or NovaSeq 6000 sequencing platforms (Illumina) ...
-
bioRxiv - Genomics 2021Quote: ... 500ng input RNA was treated using the NEBNext Human/Mouse/Rat rRNA Depletion kit and libraries were prepared following the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Paired end 2×151 bp reads were produced using the HiSeq 4000 platform (Illumina) ...
-
bioRxiv - Cancer Biology 2022Quote: ... rRNA depletion was performed using a NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and RNA was purified using Agencourt RNAClean XP Beads (New England Biolabs). Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
-
bioRxiv - Microbiology 2022Quote: ... the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used as described in the instructions with minor modifications ...
-
bioRxiv - Microbiology 2023Quote: ... the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used as described previously (31) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and the ligation mix was directly used to transform 10-beta competent Escherichia coli cells (NEB), to amplify pSG01 ...
-
bioRxiv - Molecular Biology 2024Quote: Escherichia coli strains used in this study were NEB 10-beta competent cells (New England Biolabs, Ipswich ...
-
bioRxiv - Microbiology 2022Quote: ... These primer pairs were assembled by heat-denaturation and annealing in Buffer 3.1 (NEB), then cloned into BbsI-linearized pX458 (62 ...
-
bioRxiv - Microbiology 2021Quote: ... purple (6×) (New England Biolabs). Samples were loaded into a 4-15% precast polyacrylamide gel ...
-
bioRxiv - Molecular Biology 2020Quote: ... Random Primer 6 (NEB, #S1230S), RNasin Ribonuclease inhibitor (Promega ...
-
bioRxiv - Microbiology 2024Quote: ... 6 U DNase-I (NEB) and 3 µL RNase A (NEB ...
-
bioRxiv - Genomics 2024Quote: ... and random primer 6 (NEB). The NEBNext Ultra II Non Directional RNA Second Strand Synthesis Module was subsequently used to convert single-stranded cDNA to double-stranded cDNA ...
-
bioRxiv - Synthetic Biology 2023Quote: The cell-free protein synthesis (CFPS) reactions were carried out using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs Inc) in 384-well plates (Thermofisher NUNC ...
-
bioRxiv - Microbiology 2023Quote: RdnE proteins were produced using the New England Biolabs PURExpress In Vitro Protein Synthesis Kit (New England BioLabs Inc., Ipswich MA). Template DNA contained the rdnE gene and required elements specified by the PURExpress kit ...
-
bioRxiv - Cell Biology 2023Quote: ... a PCR fragment containing the complete Nv-osk coding sequence was synthesized for protein expression with PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs) using manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2020Quote: ... rRNA depletion and library preparation for sequencing was completed with the NEBNext protocol (‘Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
-
bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared according to the protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) ...
-
bioRxiv - Immunology 2023Quote: ... Ribosomal RNA from 1000 ng total extracted RNA was depleted using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat; New England Biolabs Inc.). The remaining RNA was used to produce the sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc. ...
-
bioRxiv - Genomics 2022Quote: ... The mixture electroporated into 100 μL of 10-beta E.coli (NEB, C3020K, 2kV, 200 ohm, 25 μF) in order to achieve a transformation efficiency equal to 300-times the number of unique oligos sequences (target CFU ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Commercial competent cells were used for the K-12 DH10B strain (NEB® 10-beta high efficiency). Transformation of B REL606 and B REL607 was achieved via the chemical competence method ...
-
bioRxiv - Microbiology 2019Quote: 2–3 μL of recovered DNA was electroporated into NEB® 10-beta Electrocompetent cells (C320K, NEB) and plated on chloramphenicol LB plates ...
-
bioRxiv - Biochemistry 2024Quote: ... The resulting plasmid products were transformed into NEB 10-beta electrocompetent cells (New England Biolabs, Ipswich, MA) and purified using a ZymoPURE Midiprep Kit (Zymo Research ...
-
bioRxiv - Biochemistry 2024Quote: ... Nicking mutagenesis plasmid products were transformed into NEB 10-beta electrocompetent cells (New England Biolabs, Ipswich, MA) and purified using a ZymoPURE Midiprep Kit (Zymo Research ...
-
bioRxiv - Cancer Biology 2022Quote: ... DHFR control plasmid from PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used as backbone for subsequent ligations ...
-
bioRxiv - Immunology 2022Quote: ... U-labeled second-stranded DNAs were treated with heat-labile UDG enzyme (New England Biolabs), and ligated products were amplified with PCR by the following conditions ...
-
bioRxiv - Biophysics 2023Quote: ... followed by heat inactivation for 20 min at 80°C (Quick CIP, New England Biolabs). We added the 5’-phospho group on the synthetic parS fragment by adding a T4 kinase for 30 min at 37°C and heat-inactivated 20 min at 65°C in 1x PNK buffer supplemented with 1 mM ATP (T4 PNK ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 6 cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with 9 amplification cycles ...
-
bioRxiv - Biochemistry 2019Quote: 5’-Phosphorylated linker (Oligonucleotide K, Supplementary Table 6) was adenylated using a 5’ DNA Adenylation Kit (New England Biolabs) at 20x scale and purified by TRIzol extraction as previously described56 ...
-
bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
-
bioRxiv - Molecular Biology 2023Quote: ... 15 µL 100 µM dNTPs, 0.3 µL 50 mg/mL BSA, 6 µL blunting enzyme mix from quick blunting kit, NEB). Prior to ligation ...
-
bioRxiv - Molecular Biology 2021Quote: DNAse-treated RNA with high RIN value was used to deplete ribosomal RNA using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6350) as per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... ribosomal RNA depletion was carried on the extracted RNA using Nebnext rRNA depletion kit (Human/mouse/rat) (New England BioLabs. In, USA). Subsequently ...
-
bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared from RNA samples (150ng) using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB Cat# E7405) in conjunction with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB Cat# E7765) ...
-
bioRxiv - Biochemistry 2023Quote: ... An mRNA transcript library for Illumina sequencing was created using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs, Ipswich, MA). A NextSeq 500 sequencer (Illumina ...
-
bioRxiv - Immunology 2023Quote: ... The other pCMVtag2B vectors containing different truncated versions of rat ITCH (Fig 2F) were generated via Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Cat#E0554S) by circularizing the PCR products amplified with the primers listed in Table S2 ...
-
bioRxiv - Neuroscience 2024Quote: ... 6 μl of 10 mM MnCl and 6 μl (=2400 units) of Lambda phosphatase (#P0753, NEB). Dephosphorylation was carried out at 30°C for 30 minutes and stopped by addition of Roti Load buffer (#K929.1 ...
-
bioRxiv - Genomics 2023Quote: ... The recombination products were then electroporated into electrocompetent cells (NEB 10-beta, New England BioLabs, cat. No. C3020K) and plated onto Carbenicillin plates ...
-
bioRxiv - Systems Biology 2023Quote: ... The concentrated ligated products were then transformed into NEB 10-beta Electrocompetent E.coli (New England Biolabs, Ipswitch, MA). Three transformations were performed to increase the yield ...
-
bioRxiv - Molecular Biology 2021Quote: ... Mutant protein expression vectors were generated using the Q5 Mutagenesis Kit (New England BioLabs) and proteins overexpressed and purified the same as wild-type.
-
bioRxiv - Biochemistry 2023Quote: ... Assays were set-up using PURExpress in vitro protein synthesis kit (New England Biolabs) with a cloned full-length DHFR template ...