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Citations for New England Biolabs :
151 - 200 of 8877 citations for Mouse S formylglutathione hydrolase Esd ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... NEBNext Universal PCR primer for Illumina (5’-AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC GCT CTT CCG ATC-s-T-3’) and NEBNext Index primer for Illumina (NEB, index# 9-13 for five libraries in each repeat) ...
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bioRxiv - Physiology 2020Quote: ... and the Esp3I fragment of the pPVxRF3 vector (a gift from S. Kondo) containing Venus and 3xP3-dsRed-Express2 using NEBuilder HiFi DNA Assembly Master Mix (NEB). The combined fragment was cloned into pBluescript ...
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bioRxiv - Genomics 2021Quote: ... Methylation reactions were performed in a 100uL reaction containing 1X CutSmart Buffer and 1mM S-adenosyl-methionine (SAM, New England Biolabs) and incubated at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Either the wild-type or the mutant version of the PRNP 3’U R were inserted into the digested pmirGLO vector by performing a Gibson Assembly following manufacturer`s instructions (NEB). After transformation of the cloned vectors and purification of the DNA using EndoFree Plasmid Maxi Kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... The D614G S-protein was generated by introducing the mutation into the Wuhan reference strain via Q5 Site-directed mutagenesis (NEB). Other individual mutations were subsequently introduced into the D614G S by the same process ...
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bioRxiv - Biochemistry 2020Quote: Deglycosylation of S protein from the lysate of HEK293 cells transfected with pTwist-EF1a-nCoV-2019-S-2xStrep plasmid was performed as described previously [35] using PNGase F (New England Biolabs).
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bioRxiv - Microbiology 2021Quote: ... which were incubated with 1 μg/ml full-length S protein with His tag that had been pretreated with or without FXa (P8010L, NEB) for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... In vitro Cas9 cleavage assay was carried out using purified IVT product and Cas9 protein (Cas9 Nuclease, S. pyogenes, New England BioLabs) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Capillary tubes (0.025⍰mm inner diameter) were filled with either 0.5⍰mM hemin or 0.5⍰mM S-adenosylmethionine (SAM) (New England Biolabs, Ipswich, MA) in the motility buffer and sealed with vacuum silicone grease (Dow Corning ...
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bioRxiv - Biochemistry 2022Quote: ... accordingly to manufacturer’s instructions and 1 μg of RNA was reverse-transcribed into cDNA using M-MuLV reverse transcriptase (New England Biolabs) and random hexamers ...
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bioRxiv - Molecular Biology 2021Quote: ... 95°C/15 s and annealing/extension: 65°C/5 min) of PCR using Q5 high-fidelity DNA polymerase (NEB). Amplicons were purified using 1× AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Genomics 2020Quote: ... Reactions were performed in a 200uL reaction with 1X CutSmart Buffer and 1mM S-adenosyl-methionine (SAM, New England Biolabs) and incubated with 2.5uL enzyme at 37°C for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... Methylation reactions were performed in a 100 uL reaction with Methylation Reaction buffer (1X CutSmart Buffer,1 mM S-adenosyl-methionine (SAM, New England Biolabs)) and incubated with 2.5 uL EcoGII at 37°C for 30 minutes ...
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The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase-2 complexbioRxiv - Microbiology 2019Quote: ... of the target gene(s) was amplified and inserted into pKNG101 using a three fragment Gibson assembly reaction (HiFi Assembly, NEB). For the insertion of a deca-His encoding sequence into hyfG ...
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bioRxiv - Physiology 2021Quote: ... and the Esp3I fragment of the pPVxRF3 vector (a gift from S. Kondo) containing Venus and 3xP3-dsRed-Express2 using NEBuilder HiFi DNA Assembly Master Mix (NEB). The combined fragment was cloned into pBluescript ...
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bioRxiv - Genomics 2021Quote: ... 95°C/15 s and annealing/extension: 65°C/5 min) of PCR using Q5 high-fidelity DNA polymerase (NEB). Amplicons were purified using 1× AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Genomics 2022Quote: ... Dam activity was induced by resuspension in 100 μL DigWash supplemented with 80 μM S-adenosyl-methionine (SAM) (NEB, B9003S) and incubated at 37 °C for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: 200 nM of isolated smgRNA were injected in a pre-incubating solution of 200 nM Cas9 (S. pyogenes, NEB, M0386), 120 nM fluorescent beacon and 1 U.μL-1 murine RNase Inhibitor (NEB M0314 ...
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... by incubating the DNA construct (1 μg) with 4 units of SssI methylase and 160 μM S-adenosylmethionine (New England Biolabs) for 4 h at 37°C or mock-methylating by incubating in the absence of S-adenosylmethionine ...
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bioRxiv - Microbiology 2023Quote: ... Mutagenic PCR constructs were generated by amplifying the upstream and downstream DNA fragments flanking the gene(s) of interest followed by fusion of these fragments with the Janus Cassette using a HiFi assembly master mix (NEB). Transformation of TIGR4 with the mutagenic construct (100ng/ml ...
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bioRxiv - Plant Biology 2023Quote: ... At least 50 μg of total RNA was randomly fragmented for 125 s at 90°C to approximately 200 nt using the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs). Fragmented RNA was precipitated with 4 μl of GlycoBlue (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... concisus for 2 h at 37°C in presence of 0.4 mM S-Adenosylmethionine (SAM, New England Biolabs, Ipswich, MA). After methylation ...
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bioRxiv - Biochemistry 2023Quote: ... The same construct containing a blasticidin-S deaminase (BSD) gene in place of PAC was generated by Gibson assembly (NEB) using primers ZJ1-ZJ4 (Table S2) ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-MBP (NEB, E8032). Peroxidase and Alexa-fluor conjugated secondary antibodies were purchased from Jackson Laboratories and ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-myc (New England Biolabs), rabbit polyclonal anti- GFP (gift from Oliver Gruss ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse α-MBP (NEB, 1:10000); mouse α-Pol II CTD clone 8WG16 (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixed RNA was depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) and fragmented using NEBNext® Magnesium RNA Fragmentation Module (NEB, cat. no. E6150S) for 5 min ...
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bioRxiv - Genetics 2020Quote: ... During the experiment a fresh aliquot with 46 ng/μl sgRNA and 3.13 μM commercial Cas9 enzyme (Cas9 Nuclease, S. pyogenes, 20 μM; NEB, Ipswich, USA) was used for each day and stored on ice.
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bioRxiv - Microbiology 2019Quote: ... Typhimurium NCTC 12023 and the vector including the Tet-on system aph tetR PtetA present on p4392 occurred using oligonucleotides as listed in Table S 1 and purified by PCR purification (NEB Monarch). The PCR product encoding for sadBA and the PCR product from vector p4392 were assembled by Gibson assembly according to manufactureŕs protocol (NEB Monarch) ...
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bioRxiv - Biochemistry 2020Quote: ... UK) for the presence of the correct mutation(s) and the cassette for integration digested out with AscI (NEB, Hitchin, UK) prior to transfection.
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bioRxiv - Molecular Biology 2021Quote: ... The G1 and S/G2 population were sorted using FACSAria II cell sorter (Becton Dickinson) in PBS supplemented with RNase inhibitor (NEB, M0314L). Sorted samples were processed for scRNA-seq or cDNA-seq.
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bioRxiv - Genomics 2023Quote: Nuclei were resuspended in 200 uL of Methylation Reaction Buffer (Buffer M containing 1mM S-adenosylmethionine (SAM)) (New England BioLabs B9003S). 10uL high-concentration EcoGII was added per 1e6 nuclei and the nuclei suspension was incubated at 37°C for 30 minutes ...
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bioRxiv - Genomics 2024Quote: ... The genomic DNA was incubated in a mix containing S-Adenosyl methionine (SAM) along with the DNA adenine Methyltransferase (dam, NEB, USA) and its corresponding buffer for 4 hours at 37°C ...
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bioRxiv - Genomics 2019Quote: ... Approximately 20 ng of each DNA sample was digested with the restriction enzyme EcoRI (New England Biolabs, Ipswich, MA, U. S. A.) at 37°C for 2 hours and quality was evaluated on 1%agarose by gel electrophoresis ...
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bioRxiv - Immunology 2020Quote: ... with Poly(A) mRNA Magnetic Isolation Module (CAT#E7490S) and index PCR primers (CAT #s E7335, E7500) (New England Biolabs; Ipswich MA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... PolyA mRNA was enriched using the protocol of NEBNext ® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs #7490 S, USA). RNA sequencing libraries were then prepared using Hieff NGS® Ultima Dual-mode mRNA Library Prep Kit (Yeasen ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse monoclonal anti-MBP (1:2000, NEB), rabbit polyclonal anti-Spo11 (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: ... MBP (mouse, 1:30,000, New England Biolabs), FLAG (mouse ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared in the Duke Center for Genomic and Computational Biology’s Sequencing and Genomic Technologies Core Facility using the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module (NEB, catalog no. E6421) and SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-MBP (New England Biolabs, RRID:AB_ 1559738), used at 1:5000 ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse anti-PPARα (1:25, Arigo Biolabs ARG55240). Alexa Fluor conjugated secondary antibodies (ThermoFisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was mixed with Reaction Mix 1 (9:1 of 12 uM NEB RT random 6N-S to 12 uM oligo-dT(20), 12uM dNTPs ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... anti-MBP mouse 1/80,000 (E8032S, New England Biolabs). Western Blots were quantified using Fiji ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-myc (NEB/Cell Signaling, 2276; 1:1500) o/n at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary mouse antibodies against MBP (NEB E8032L, 1:5000) and RAD51 (Novus Biologicals 14b4 ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse monoclonal anti Pan-Keratin (clone C11, NEB, 4545S). Primary antibodies were incubated with sections overnight at 4°C except for anti-CD3 ...
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bioRxiv - Genomics 2022Quote: Ribosomal RNA depletion kit (rRNA Depletion Kit, E6310L, NEB)
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with mouse anti-MBP (NEB, 1:500), followed by washing and subsequent incubation with goat anti-mouse IgG ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were stained with mouse anti-MBP (NEB, 1:500), followed by washing and subsequent incubation with goat anti-mouse IgG ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse α-myc (New England Biolabs, clone 9B11, 1:2,000), rabbit α-pol-I largest subunit 15 (1:100 ...