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Citations for New England Biolabs :
1901 - 1950 of 9843 citations for Swine IL 4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... mRNA synthesis reactions were cleaned using Monarch RNA Cleanup Kit (NEB: #T2040L) and visualized using agarose gel-electrophoresis ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were transcribed with T7 HiScribe kit (New England Biolabs) and transfected together with TrueCas9 protein v2 (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Point mutations were made using the Q5 Site-Directed Mutagenesis Kit (NEB) with mutagenic primers or mutagenic double-stranded DNA fragments (gBlocks™ ...
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bioRxiv - Cell Biology 2022Quote: ... and sgRNAs were purified with Monarch RNA Cleanup Kit (New England Biolabs). sgRNA sequences and PCR primers used in this study are listed in Supplementary Table 2.
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bioRxiv - Genomics 2022Quote: RNA extraction was performed using the Monarch Total RNA Miniprep Kit (NEB) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2022Quote: ... polyadenylated with the E.coli Poly (A) polymerase Kit (NEB, cat. no. M0276L), and purified with the RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were cleaned (Monarch PCR & DNA Cleanup Kit, NEB cat. T1030S) and Sanger-sequenced ...
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bioRxiv - Microbiology 2022Quote: ... Products were PCR cleaned up with Monarch PCR & DNA cleanup kit (NEB), ligated into pCR2.1-TOPO vector (Thermo fisher ...
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bioRxiv - Microbiology 2022Quote: ... or fragment insertion using the Gibson assembly Cloning kit (New England Biolabs) were employed according to the supplier’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Mutagenesis of complement templates was performed using the Q5 Mutagenesis kit (NEB) with primers referenced in the primer table S1.
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bioRxiv - Microbiology 2022Quote: ... reverse transcribed and amplified with OneTaq one-step RT-PCR kit (NEB) for verification by Sanger sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... The guide RNAs (gRNAs) were generated by EnGen sgRNA Synthesis Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... mRNA was isolated using the Magnetic mRNA Isolation Kit (New England Biolabs), followed by rRNA depletion using the RiboMinus Eukaryote System v2 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed according to NEBNext Small RNA Library Prep kit (NEB) protocol ...
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bioRxiv - Microbiology 2023Quote: ... All other plasmids were constructed using a HiFi DNA assembly kit (NEB). All plasmids were confirmed by Sanger sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting RNA was purified using the RNA cleanup kit (#T2040, NEB) and injected into 1-2 cell stage gcna/acrc maternal mutant embryos or into control WT embryos (1nl of the mRNA solution ...
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bioRxiv - Systems Biology 2022Quote: ... and 2μL of NEBridge Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602L), added in that order ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated using a Q5 site directed mutagenesis kit (New England Biolabs) according to the manufacturer’s instructions and expressed and purified as for VCB.
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bioRxiv - Molecular Biology 2022Quote: ... and purified with the Monarch Plasmid Miniprep Kit (T1010, New England Biolabs). Plasmids were digested with restriction enzymes to generate linearized templates for in vitro transcription ...
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bioRxiv - Microbiology 2022Quote: ... or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) or LightCycler® Multiplex RNA Virus Master (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... and Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, U.S.A.) protocol ...
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bioRxiv - Cell Biology 2022Quote: ... or homologous recombination based NEBuilder® HiFi DNA Assembly kit (NEB, MA). Site-directed mutagenesis was carried out using QuikChange QuikChange Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Plant Biology 2024Quote: ... Using NEBNext Ultra II kits for Illumina (New England BioLabs, Ipswich, MA), each pool was indexed with a unique NEBNext i7 adapter and an i5 adapter containing a degenerate barcode (for removal of PCR duplicates ...
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bioRxiv - Synthetic Biology 2024Quote: ... The HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs) was used for in vitro transcription according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs). Gel fragments were extracted using GeneJet Gel extraction kit (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid libraries are generated using Golden Gate Assembly Kits (NEB, BsaI-HFv2). The reactions undergo a cycling protocol of 30 alternating 5-minute 37°C and 16°C cycles followed by a final 60°C 5-minute hold ...
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bioRxiv - Immunology 2024Quote: ... PCR and Gibson Assembly® Cloning Kit (New England Biolabs, Ipswich, MA). The product was transformed in E ...
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bioRxiv - Genomics 2024Quote: ... using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA). These libraries were then sequenced to produce 150-bp paired-end reads using a NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Luna Universal One-Step RT-qPCR kit (New England Biolabs) with the following primers:
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bioRxiv - Microbiology 2024Quote: ... The reaction system was prepared according to the kit’s requirements (Yugong Biolabs) and fluorescence quantitative analysis was performed using the ABI 7500 PCR system (Applied Biosystems) ...
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bioRxiv - Zoology 2024Quote: ... The Monarch® RNA purification kit (New England Biolabs, Whitby, ON, Canada) was used to purify synthesized dsRNA following the manufacturer’s recommendation ...
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bioRxiv - Physiology 2024Quote: ... cDNA was synthesized using a cDNA Reverse Transcriptase Kit (New England Biolabs). TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... products were purified with the Monarch PCR DNA cleanup kit (NEB, Inc.) and analyzed by endpoint PCR ...
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bioRxiv - Microbiology 2023Quote: ... purification (Monarch PCR & DNA Cleanup Kit, New England Biolabs Cat. No. T1030), and ligation with T4 DNA ligase (New England Biolabs Cat ...
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bioRxiv - Biochemistry 2024Quote: ... Point mutations were generated using the Q5 Site-Directed Mutagenesis Kit (NEB). Primer sequences and plasmid construct information is listed in Supplementary Table 1.
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II kit (NEB E7645) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs E5520S). The fusion construct was then amplified by PCR and inserted into the multiple-cloning site of the lentiviral vector pLEX using the same NEBuilder cloning kit to generate pLEX-mNFL-GFP ...
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bioRxiv - Microbiology 2024Quote: ... they were purified using Monarch DNA Gel Extraction kit (New England Biolabs) and assembled by two-step PCR (supplementary methods ...
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bioRxiv - Microbiology 2024Quote: In vitro transcription/translation assays were conducted using the PURExpress kit (NEB) according to the manufacturer’s protocol with a 2-hour incubation at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was isolated using a Monarch Total RNA Miniprep Kit (NEB), RNA quantity and quality were determined with a NanoDrop OneC Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: dsRNA was generated by in vitro transcription (NEB T7 E2050s transcription kit) from PCR products generated by the following forward and reverse primers (separated by comma):
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bioRxiv - Biochemistry 2023Quote: ... PRIMPOL S255D mutant was generated using Q5 site-directed mutagenesis kit (NEB). The pCS2 NLS-mCherry-PRIMPOL WT or S255D vectors were used for transfection in U2OS PRIMPOL KO cells using TransIT (Mirus ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using the ProtoScript First Stand cDNA Synthesis Kit (NEB). The obtained cDNA was diluted 10-fold to 200 μL with nuclease-free water ...
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bioRxiv - Cell Biology 2024Quote: ... The NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA) was used for generating the RNA-Seq library for each sample ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using NEBNext small RNA sequencing kit (New England Biolabs) or NextFlex small RNA sequencing kit (Perkin Elmer ...
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bioRxiv - Molecular Biology 2023Quote: ... Inserts were acquired by PCR using KOD Hotstart Mastermix from NovaGen and purified from gel using Monarch DNA Gel Extraction kit from NEB. All cloning was performed in E ...
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bioRxiv - Synthetic Biology 2023Quote: ... cDNA was synthesized using a LunaScript® RT SuperMix Kit (NEB E3010), and real time PCR was run on a Bio-Rad CFX96 Touch Real-Time PCR Detection System using an iTaq Universal SYBR Green Supermix (BioRad #1725121) ...
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bioRxiv - Developmental Biology 2024Quote: ... IVT sgRNA products were purified Monarch PCR and DNA Cleanup Kit (NEB) and quantified ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA was purified with the NEB mRNA isolation kit (New England Biolabs). Extracted RNA was treated with DNase I (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... We used a magnetic mRNA isolation kit (New England BioLabs, Ipswich, MA) to enrich samples for mRNA before proceeding to library preparation ...