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Citations for New England Biolabs :
1901 - 1950 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and Gibson assembly with NEBuilder HiFi DNA Assembly Cloning kit (New England Biolabs) into pFastBac vector (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... and then reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... followed by ribosomal RNA (rRNA) depletion using NEBNext rRNA Depletion Kit (E6310, NEB). The ribosomal-depleted RNA was used to make the RNA-seq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7760 ...
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bioRxiv - Plant Biology 2022Quote: ... and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was performed using NovaSeq 6000 (Illumina ...
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bioRxiv - Neuroscience 2022Quote: ... Single guide RNA (sgRNA) was synthesized using EnGen sgRNA Synthesis Kit (NEB, E3322) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: cDNA was prepared using LunaScript® RT SuperMix Kit (New England Biolabs, #E3010L), together with no-RT control reaction and no template controls ...
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bioRxiv - Cell Biology 2022Quote: ... Sequencing libraries were prepared using the NEBNext® Ultra II kit (NEB, E7645S) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was then purified with Monarch PCR & DNA Cleanup Kit (New England BioLabs). Libraries were prepared using Q5 High-Fidelity 2X Master Mix NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... Amplified libraries were purified with Monarch PCR & DNA Cleanup Kit (New England BioLabs) and sequences Illumina NextSeq 2000 system at Boston University Microarray and Sequencing core ...
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bioRxiv - Developmental Biology 2024Quote: ... The Tg(UAS:GFP-P2A-ppp3ccacA) has been generated by ligation (KLD kit, NEB) of PCR fragments GFP-P2A-ppp3ccacA (IM459/IM460 ...
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bioRxiv - Biochemistry 2024Quote: ... Fragments were ligated together with NEBuilder HiFi DNA Assembly Kit (New England Biolabs). Then Escherichia coli DH5α competent cells were transformed with the ligation mixtures and the cells containing assembled plasmid were selected on an agar medium supplemented with 100 mg/l ampicillin ...
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bioRxiv - Bioengineering 2024Quote: ... The mRNA was purified using the Monarch RNA Cleanup Kit (NEB, MA, UK). Followed reagents were used for IL-15 superagonist characterization ...
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bioRxiv - Bioengineering 2024Quote: ... Transcription reactions were purified using the Monarch RNA cleanup kit (New England Biolabs). mRNA concentration was quantified by nanodrop spectrophotometer ...
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bioRxiv - Bioengineering 2024Quote: ... Synthesized mRNA was purified using the Monarch RNA cleanup kit (New England Biolabs) and stored at −80°C ...
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bioRxiv - Cell Biology 2024Quote: ... These fragments were assembled using a NEBuilder® HiFi DNA Assembly kit (NEB) to create a pET24a-anti-ALFA-std expression vector ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were made using the Small RNA library preparation kit NEBNext (NEB) Small RNA library prep set according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using NEBNext Ultra II Library Prep Kit for Illumina (#E7645, New England Biolabs). The sub-libraries were amplified and barcoded using a ProFlex PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... The I304N mutant FMRP was generated using Q5 Site-Directed Mutagenesis Kit (NEB). G3BP1-GFP plasmid was a gift from Jeff Chao (Addgene plasmid #119950) ...
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bioRxiv - Genetics 2023Quote: ... and their concentration was determined using the NEBNext Lib Quant Kit (E7630, NEB). The libraries were sequenced on an Illumina NextSeq 550 ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was then extracted using the Monarch gDNA Purification Kit (NEB T3010S) following the standard protocol but with elution using 50 µL 25 mM K3BO3 at pH 7.0 ...
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bioRxiv - Cell Biology 2024Quote: Libraries were generated using the NEBNext Ultra II DNA Library Prep kit (NEB). For transcription factors (BMI1 and RING1B) ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR products were gel-purified with Monarch DNA Gel Extraction Kit (NEB) and used for the ligation reactions ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplicons were purified using the Monarch Gel Extraction Kit (New England Biolabs, T1030) before sequencing using an Illumina MiSeq ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA extraction was performed using the Monarch total RNA Miniprep Kit (NEB, T2010S) following the manufacture recommendations and RNA samples were stored at −80°C until use.
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bioRxiv - Microbiology 2023Quote: ... and then an assembly reaction using the NEBuilder HiFi DNA Assembly Kit (NEB) was performed to insert the Ultramers into the linearized vector and re-circularize ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... Libraries were prepared using the NEBNext® Enzymatic Methyl-seq Kit (NEB, #M7634) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and mutant histone H2A-K119C was generated using the Q5 mutagenesis kit (NEB). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... before library construction using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB). The libraries were sequenced on an Illumina NoveSeq 6000-PE150 ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (NEB) followed by Illumina 150 Paired-End sequencing in biological replicates ...
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bioRxiv - Plant Biology 2024Quote: ... Luciferase RNA was then purified using Monarch RNA cleanup kit (New England Biolabs) according to Manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Resulting mRNA products were purified using Monarch RNA Cleanup Kit (New England BioLabs), visualized on 1% TBE gel ...
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bioRxiv - Evolutionary Biology 2024Quote: ... intermedium cells using the Monarch® Total RNA Miniprep Kit (New England Biolabs). For the nutrient medium condition ...
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bioRxiv - Genomics 2024Quote: ... Total RNA was extracted with the RNA Miniprep Kit (New England Biolabs, #T2010) according to the manufacturer’s instructions and used for standard strand-specific Illumina library prep ...
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bioRxiv - Genetics 2024Quote: ... Mutant constructs were constructed using a Q5 site-directed mutagenesis kit from NEB. To introduce specific mutations into the constructs ...
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bioRxiv - Genomics 2024Quote: ... the DNA fragments were purified using the Monarch PCR & DNA Cleanup Kit (NEB). Libraries were constructed using Illumina Nextera Dual Indexing ...
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bioRxiv - Genomics 2024Quote: ... using the Monarch DNA purification kit (New England Biolabs, Inc., Rowley, MA, USA) to extract genomic DNA from the original frozen whole blood samples ...
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bioRxiv - Immunology 2024Quote: ... libraries were prepared using NEBNext Ultra II Library Prep Kit for Illumina (NEB), size selected (150-500 bp) ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized using the ProtoScriptII First Strand cDNA Synthesis Kit (NEB; E6560S), following the manufacturer’s protocol with the Random Primer Mix and the RNA denaturation step ...
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bioRxiv - Cell Biology 2024Quote: ... and A549 Chk2-/- cells using the Monarch® Genomic DNA Purification Kit (NEB). Prior to Sanger sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760) was then used to prepare libraries for paired-end (2x150bp ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using the Monarch PCR & DNA Cleanup Kit (NEB) and sent for Sanger sequencing (GENEWIZ).
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bioRxiv - Microbiology 2023Quote: ... and cDNA synthesis performed using a LunaScript RT SuperMix kit (New England Biolabs). RT-qPCR was performed on the resulting cDNA using a LightCycler 96 Real-Time PCR system (Roche ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were cleaned using the Monarch PCR & DNA cleanup kit (NEB, T1030L). VSG sequences were determined by amplicon nanopore sequencing performed by Plasmidsaurus using Oxford Nanopore Technology with custom analysis and annotation or Sanger sequencing with the sequencing primer (5’-AGAGAATACTAAGCTAGTTGGC-3’ ...
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bioRxiv - Immunology 2024Quote: ... plasmid and inserted with NEBuilder HiFi Assembly cloning kit (#E5520S, New England Biolabs).
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bioRxiv - Biophysics 2023Quote: ... DNA was purified using Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... RNA was purified using Monarch RNA Clean-up Kit (50 μg) (NEB, T2040S).
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bioRxiv - Genomics 2024Quote: ... the facility used the Ultra II DNA kit for Illumina (New England Biolabs), which included an enzymatic shearing as a first step ...
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bioRxiv - Biochemistry 2023Quote: ... the plasmid above was modified using the Q5 Site-Directed Mutagenesis Kit (NEB) along with the following primers ...
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bioRxiv - Genetics 2023Quote: ... and mRNA was transcribed using the HiScribe T7 high yield RNA kit (NEB) using N1-methylpseudouridine (Trilink ...
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bioRxiv - Cell Biology 2023Quote: ... using the NEBNext UltraTM RNA Library Prep Kit for Illumina® (NEB, USA), with enrichment for mRNA using oligo(dT)-magnetic beads ...