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Citations for New England Biolabs :
1901 - 1950 of 3340 citations for 7 Decyn 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and ligated into pLKO.1 vectors using T4 DNA ligase and manufacturer protocols (New England Biolabs). DNA was transformed into Stbl3 bacteria for plasmid preparation (Thermo Fisher).
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bioRxiv - Cell Biology 2021Quote: ... Receptors were surface labelled by addition of Snap-Cell 647 SiR (1:1000, New England Biolabs) to the media for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of crude mitochondria were mixed with 1/10 volume gel loading dye (7025, NEB) and subjected to Semi-Denaturing Detergent Agarose Gel Electrophoresis (SDD-AGE) ...
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bioRxiv - Microbiology 2020Quote: ... the supernatant was collected and mixed with 1 ml of amylose agarose resin (New England Biolabs) pre-equilibrated with buffer A per 50 ml of cell homogenate ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL xonuclease I and 2.44 μL Antarctic Phosphatase buffer (New England BioLabs GmbH, Frankfurt, Germany) to 20 μL of the pooled PCR product ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM ATP and 0.85 U of T4 DNA ligase (67 U/μL) (New England BioLabs). Ligation was performed at 37°C for 2 hours and 16°C for 16 hours ...
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bioRxiv - Genomics 2020Quote: ... each eluted insert was mixed with 50ng pDXinit-PAC in a molar ratio of 1:5 (vector:insert) in 10μl reactions and digested with 0.5μl SapI (NEB) for 1h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then treated 10 min with 1 U DNase I (New England BioLabs, Ipswich, MA) at 37°C ...
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bioRxiv - Microbiology 2021Quote: One microgram of full-length S protein was treated with 1 μg of FXa (P8010L, NEB), furin (P8077S ...
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bioRxiv - Microbiology 2021Quote: ... The ATP hydrolysis reaction was performed by incubating 0.3 µM HelR with 1 mM ATP (NEB), in a buffer containing 40 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... In case of any potential overlap we treated our PCR reactions with 1 µL DpnI (NEB) to eliminate the donor plasmid ...
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bioRxiv - Microbiology 2021Quote: ... For reverse transcription (RT) 1 µg RNA was digested with 2 U of DNase I (NEB). After heat inactivation of the DNase at 70 °C for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... Annealed circles were ligated with the addition of 1 μL of T4 DNA ligase (NEB M0202S) at room temperature for ∼4 hours ...
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bioRxiv - Cell Biology 2022Quote: ... for 30 min at room temperature and sequentially incubated with 1 µM Alexa647-SNAP-dye (NEB) and 1 mM DTT in PBS for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... One microgram (1 μg) total RNA was used as an input for mRNA isolation (NEB E7490S), followed by library preparation using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB E7760) ...
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bioRxiv - Cancer Biology 2022Quote: ... Reaction was supplemented with 1 mM dATP and 0.1 mM dCTP and 0.1 mM dGTP (NEB) and performed as follows in a thermocycler ...
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bioRxiv - Bioengineering 2022Quote: ... 1x RNA polymerase buffer and ∼1 U/µl of T7 RNA polymerase (New England Biolabs, Inc.). The samples were incubated at 37 °C for 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... cleared lysate was incubated without or with PNGase F (NEB, 1 μl per 40 μl lysate) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... PV stock solution (1 mM) was prepared by adding 10 μl of 100 mM vanadate (NEB) and 50 μl of 100 mM hydrogen peroxide (diluted from a 30 % stock in 20 mM HEPES ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Recovered footprints were dephosphorylated in a 15 µL reaction mixture (1 µL T4 PNK (NEB, M0201S), 10X T4 PNK buffer ...
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bioRxiv - Microbiology 2021Quote: ... immediately rescued in 1 ml of 37°C SOC outgrowth medium (New England Biolabs, cat#B9020S) and incubated with shaking at 37°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL Exonuclease I and 2.44 μL Antarctic Phosphatase buffer (New England BioLabs GmbH, Frankfurt, Germany) to 20 μl of the pooled PCR product ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 uL of cDNA was used in a 20 uL reaction with Phusion polymerase (NEB, M03530). The following cycling conditions were used for PCR prior to agarose gel electrophoresis ...
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bioRxiv - Systems Biology 2022Quote: ... for 48 hours and MtTether-SNAP was stained with 1:2000 SNAP-647 SiR (NEB, s9102) for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: ... cells were suspended in 1 ml of super optimal broth with catabolite repression (S.O.C. medium) (NEB) and incubated at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... and indexed common primers (Vazyme, Cat.No.TD202) in 1 x Q5 High-Fidelity Master Mix (NEB, Cat.No.M0492). Then index PCR was performed as follow ...
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bioRxiv - Cell Biology 2019Quote: ... 40 nM down primer and 5 μM dNTP in 17 μl 1 x CutSmart buffer (NEB). The RNA and primers were incubated at a temperature gradient ...
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bioRxiv - Cell Biology 2020Quote: ... gel purified and ligated with pENTR (w158-1)-backbone by Quick Ligation kit (New England Biolabs) to create pENTR-GRET ...
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bioRxiv - Synthetic Biology 2020Quote: ... and immediately adding 1 U of β-agarase I per 100 ul of molten gel (NEB). The mixture was incubated at 42°C for 60 min to release DNA bound in the agarose matrix ...
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bioRxiv - Cancer Biology 2020Quote: shRNA was cloned into pLKO.1 vector using restriction enzyme sites AgeI (NEB, Cat. No. R3552) and EcoRI (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units ...
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bioRxiv - Molecular Biology 2021Quote: ... This was followed by the addition of 1 µl 10x T4 RNA ligase reaction buffer (NEB), 1 µl NEB T4 RNA Ligase2 ...
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bioRxiv - Microbiology 2020Quote: ... Biotin-labeled RNP lysates were supplemented with streptavidin beads (1 mL per replicate, New England Biolabs) and captured by rotating at room temperature overnight ...
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bioRxiv - Cell Biology 2020Quote: β1-tubulin wild type construct was generated by the gibson assembly (HiFi Kit, New England Biolabs) of a TubB1 sequence fragment synthesised as a gBlock by Integrated DNA Technologies (IDT ...
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bioRxiv - Biochemistry 2020Quote: ... The polymerase chain reaction (PCR) included 1-unit Phusion High-Fidelity DNA Polymerase (New England Biolabs), 1x Phusion buffer ...
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bioRxiv - Genomics 2021Quote: ... 25 ng of cDNA was digested with 0.5 µl 1:40 diluted RNaseH (New England Biolabs) for 20 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10uM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1μL 10uM NEBNext Universal PCR Primer in 20 μL total volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10uM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1μL 10uM NEBNext Universal PCR Primer in 20 μL total volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10uM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1μL 10uM NEBNext Universal PCR Primer in 20 μL total volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10uM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1μL 10uM NEBNext Universal PCR Primer in 20 μL total volume ...
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bioRxiv - Genomics 2021Quote: ... ribosomal RNA depletion was performed using 1 μg RNA using NEBNext rRNA Depletion kit (NEB#6310). Concentration of final libraries was measured using Qubit 2.0 Fluorometer in combination with Qubit dsDNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Ligation was performed by adding 1 unit of T4 RNA Ligase 2 enzyme (New England Biolabs) in 10 μl of 1X reaction buffer and incubating the reaction at 37°C for 60 min ...
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bioRxiv - Biochemistry 2021Quote: Native glycoproteins (10 μg) were digested with 1 U of IMPa (O-Glycoprotease, New England Biolabs) in 50 μL 20 mM Tris-HCl ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Desialylation of ACE2-wt-Fc was performed with 2500 U mL-1 neuraminidase (New England Biolabs) in 50 mM sodium citrate (pH 5.0 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were labelled with BG-S-S-649 (1 μM, a gift from New England Biolabs). After washing ...
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bioRxiv - Biochemistry 2020Quote: ... Annealed circles were ligated with the addition of 1 μL of T4 DNA ligase (NEB M0202S) at room temperature for ~4 hours ...
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bioRxiv - Genetics 2021Quote: ... for 1 hour at 37°C into pPB_U6::gRNA_EF1a::BFP-Puro digested with BlpI (NEB #R0585S) and BstXI (NEB #R0113S) ...
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bioRxiv - Neuroscience 2020Quote: ... After overnight incubation at 4°C in PBS-Perm with rabbit anti-GFP (1:1000, Biolabs) and mouse anti-MAP2 (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... Sonicated salmon sperm DNA was then diluted to 1 mg/ml in Terminal Transferase buffer (NEB) with 0.25 mM CoCl2 ...
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bioRxiv - Biophysics 2021Quote: ... The cells were then incubated with the SNAP substrate (Alexa 647 – benzylguanine, 1 µM, NEB#S9136S) in 0.5% BSA + 1mM DTT at room temperature for 2 hours ...